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At least 397 records · Page 22Linked to original sources
PubMed1981

Astrocytes of the cerebral cortex in hepatosplenic schistosomiasis mansoni and in liver cirrhosis. A morphological, quantitative and karyometric study.

A morphological, quantitative and karyometric study of astrocytes of the cerebral cortex in patients with liver cirrhosis, hepatosplenic schistosomiasis mansoni and controls is reported. Cell proliferation was commonly seen but there was no significant increase in number of astrocytes in either cirrhosis and schistosomiasis groups. A highly significant increase in astrocyte nuclear volume in cirrhosis and schistosomiasis in relation to controls was observed. The astrocyte average nuclear volume in the cirrhotics was also significantly increased in relation to the schistosomiasis group. From the present data and those reported by other investigators it may be concluded that under normal conditions the astrocyte population is continually reforming and it proliferates in liver cirrhosis and hepatosplenic schistosomiasis. It seems that the morphological, quantitative and karyometric astrocyte changes in schistosomiasis may be the result of the same factors as those previously described for liver cirrhosis and experimental portacaval shunt.

Astrocytes↗
PubMed1989

Evidence of an acquired increase in mitogenesis in streptozotocin-diabetic rats, apparently relating to some tissue factor.

We have previously reported that rats which have been suffering from streptozotocin-diabetes for 4 weeks show a supranormal mast cell mediated mitogenesis in mesenteric windows and in the skin; this late emerging, augmented mitogenic responsiveness appears, to be unaffected by insulin per se. To test whether this increased proliferogenic response is effected by some acquired quality within the tissue rather than a systemic factor in the blood, we studied mast cell mediated mitogenesis in organ-cultured intact mesenteric windows from rats with diabetes of 4 weeks' duration, using a biochemically-defined serum-free growth medium. Mast cells were activated by Compound 48/80 and their secretion was quantified biochemically in terms of histamine release. The mast cell-dependent mitogenic reaction in the predominant, morphologically discrete fibroblasts and mesothelial cells was quantified photometrically using Feulgen-absorption analysis of individual cell nuclei, and by determination of the mitotic index. Both types of target cell responded to a significantly greater degree mitogenically in diabetic compared with control tissue. This finding suggests that a considerable part of the increased mitogenic responsiveness previously observed in diabetic animals in vivo is causally related to some tissue-bound, i.e., cellular and/or extracellular factor(s) acquired during the course of the disease.

Animals↗
PubMed1985

Caryometry and nuclear ultrastructure of cultured human breast cancer cells (FAM) after oestradiol administration.

In a breast cancer cell line (FAM) isolated and characterized in our laboratory, we previously observed that oestradiol (E2) did not affect cell multiplication in vitro. In this work, we examined whether these cells bearing oestrogen and progesterone receptors, were oestrogen (E)-sensitive in spite of their lack of growth response. We observed that E2 administration resulted in an increase in protein synthesis characterized by enhancement of progesterone receptors, creatine-phosphokinase and plasminogen activator. Thus these FAm cells were true E-responsive target cells and we studied their nuclear size and ultrastructure to determine if E2 stimulation induced the same important changes as described in vivo. We observed no significant differences between control and E2-treated cells. In these breast cancer cells in vitro, E does not act on DNA-auto-reproduction but only on transcriptional activity. Thus, there is only a limited number of activated genes and no gross nuclear morphological changes. In these cultured oestrogen target cells, ultrastructural nuclear changes are not a marker of hormone action, unlike the situation in vivo where E also acts in triggering cell multiplication.

Breast Neoplasms↗
PubMed1976

Changes in size, DNA content, and nuclear ploidy of rat liver produced by the environmental microflora.

Effects of the environment microflora on rat liver were studied in the present investigation. Rats born under sterile conditions were bred either in a germfree or in a "SPF" environment. At 12 weeks of age the liver of the SPF rats was larger by 20% and contained more DNA than germfree rat liver. Volume analysis, by means of a particle counter, of isolated nuclei suggested that the increase of DNA was due to an increase of hepatic nuclear ploidy. The findings show that infections may influence liver size and ploidy. They indicate that the state of infection should be controlled in animal studies which concern the regulation of liver growth and ploidy or which use liver weight and DNA as reference standards.

Animals↗
PubMed1976

Classification of cells in vaginal smears with television microscopy.

Properties of 924 cells were measured using a television microscope. A program was written using measured properties discriminating between normal and malignant cells. An investigation of the number of free-lying whole cells and free-lying nuclei on a malignant smear is presented. It is shown that it is possible to get a rather accurate discrimination for single cells using television microscopy with only 10 grey-levels and measuring only density of nucleus, cytoplasm and size of nucleus. However, if classification of single cells should be applied to classify an entire smear, the accuracy in the classification of the single cells must be very high.

Cell Nucleus↗
PubMed1983

Discrimination of various epithelia by simple morphometric evaluation of the basal cell layer. A light microscopic analysis of pseudostratified, metaplastic and dysplastic nasal epithelium in nickel workers.

This study presents a simple morphometric method for objective classification of pseudostratified, various types of metaplastic, and dysplastic epithelium by evaluation of cellular features in the basal layer only. Fifty-four biopsy specimens were taken for diagnostic reasons from the nasal mucosa of nickel workers, and semithin toluidine-blue-stained sections were analysed. The most sensitive parameters in distinguishing between the various types of epithelium were: (i) the transverse nuclear diameter, (ii) the size of the nucleoli and (iii) the basal cell width expressed as an index weighted towards the cell profiles with the broadest attachment face on the basal lamina. A combination of these three parameters allows a clear separation between dysplastic, metaplastic and pseudostratified epithelium. The sequential increase in these parameters from pseudostratified epithelium through two histologically distinguishable stages of metaplasia (stratified cuboidal and mixed stratified cuboidal/stratified squamous epithelium) to fully developed squamous epithelium supports the concept that metaplasia develops gradually. The continuous increase in these parameters from metaplasia to dysplasia further suggests that metaplasia is a necessary step in the development of nasal epithelial dysplasia. This morphometric model appears especially useful in monitoring small sequential epithelial changes, and might also be used for evaluating other types of epithelia.

Biopsy↗
PubMed1982

Age-related alterations in the size of human hepatocytes. A study of mononuclear and binucleate cells.

Age-related alterations in the size of human hepatocytes (both mononuclear and binucleate forms), were studied in histological sections and in separated cells and nuclei using cytophotometrical and microspectrophotometrical methods. The following results were obtained: 1. The volume of nuclear DNA increased in proportion to nuclear size. The increase occurred in a group pattern reflecting nuclear polyploidization. 2. Cell size increased in proportion to nuclear size. Tetraploid cells (4C) were roughly two times greater than diploid cells (2C). 3. In most of the binucleate cells examined, the ploidy class of the two nuclei in a binucleate cell was observed to be equal. Heterogeneity of the ploidy class among the nuclei of a binucleate cell was present in less than 1% of total binucleate cells examined. The nuclear DNA volume of individual nuclei in binucleate cells appeared to be the same as that of mononuclear cells. 4. The cell size of binucleate cells corresponded with that of mononuclear cells whose ploidy class was the same as the sum of the ploidy classes of two nuclei of a binucleate cell. 5. The incidence of binucleate cells in the lobular periphery was about 4 to 6% in the third decade, and increased slightly with age up to 5 to 7% in the tenth decade. 6. The incidence of binucleate cells in the liver at different ages followed a similar pattern to that observed in mononuclear cells whose ploidy class was half of the sum of ploidy classes of the two nuclei of the binucleate cell.

Adult↗