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PubMed2003

Susceptibility to IVM in a field strain of Haemonchus contortus subjected to four treatments in a closed sheep-goat flock in Kenya.

Susceptibility to IVM (IVM) of "strain A" Haemonchus contortus which had been exposed to IVM four times over a 2-year period was compared to IVM susceptibility of "strain C" H. contortus which had no prior field exposure to IVM, by in vivo and in vitro methods. In vivo, the percentage reduction in faecal egg counts (FEC) and the total worm counts (TWC) were compared between control animals (lambs and kids) and animals treated with low dose IVM (20 microg/kg). In vitro susceptibility to IVM was evaluated by larval migration inhibition (LMI) after the two strains of H. contortus were exposed to different concentrations of IVM. The dose response, measured as the proportion of larvae inhibited from migrating, was used to estimate LD(50). Although differences in response to IVM in the in vivo determinations were not significant, "strain A" H. contortus had a significantly higher LD(50) than "strain C" in the LMI assay. Coincident with the conduct of the in vivo experiment, it was observed that "strain A" H. contortus established and survived better than "strain C" in the control lambs.

Animals↗
PubMed2003

Amphidial structure of ivermectin-resistant and susceptible laboratory and field strains of Haemonchus contortus.

The development of anthelmintic resistance by nematode parasites is a growing problem for veterinarians, pet owners, and producers. The intensive use of the macrocyclic lactones for the treatment of a variety of parasitic diseases has hastened the development of resistance to this family of parasiticides. As a result, resistance to ivermectin, moxidectin, nemadectin, and doramectin by Haemonchus contortus has been documented throughout the world. Sensory neurons located in the cephalic end of nematodes are in close contact with the external environment. Through these neurons, important chemical and thermal cues are gathered by the parasite. Examination of serial electron micrographs of ivermectin-susceptible and ivermectin-resistant H. contortus allows for comparison of neuronal structure, arrangement of neurons within the amphidial channel, and distance of the tip of the dendritic processes to the amphidial pore. The latter of these characteristics provides a useful means by which to compare the association between the neurons and the external environment of the worm. Comparison of parental laboratory strains of ivermectin-susceptible strains of H. contortus with related selected, ivermectin-resistant strains and with a wild-type ivermectin-susceptible field strain of H. contortus from Louisiana reveal that the ivermectin-resistant worms examined have markedly shorter sensory cilia than their ivermectin-susceptible parental counterparts. Additionally, the amphidial neurons of ivermectin-resistant worms are characterized by generalized degeneration and loss of detail, whereas other neurons outside of the channels, such as the labial and cephalic neurons, are normal in structure. These findings raise a number of questions regarding the relationship between amphidial structure and ivermectin resistance as well as the role of amphids as a means of entry for ivermectin. While shortened amphidial sensilla are associated with ivermectin resistance, it remains unclear if such a structural modification facilitates survival of nematodes exposed to macrocyclic lactones.

Animals↗
PubMed2003

Effects of condensed tannins on goats experimentally infected with Haemonchus contortus.

Although the use of tanniferous plants or condensed tannins as an alternative to anthelmintics to control gastrointestinal nematodes has been largely documented in sheep, studies remain scarce in goats. The objective of this study was therefore to assess the possible impact of condensed tannins in goats infected with adult Haemonchus contortus. Two groups of cull goats were experimentally infected with 10.000 L3 of H. contortus. After 4 weeks, quebracho extracts, representing 5% of the diet DM, were administered for 8 days to one of the two groups. Goats of the second group remained as controls. One week after the end of quebracho administration, the goats were euthanised. Individual egg excretion and pathophysiological parameters were measured weekly during the study. At the end of the study, worm counts were assessed and histological samples from the abomasa were taken to count the numbers of mucosal mast cells, globule leukocytes and eosinophils. The administration of tannins was associated with a significant decrease in egg excretion, which persisted until the end of experiment. This reduction was not associated with any difference in worm number but with a significant decrease in female fecundity. No significant changes in the mucosal density of the three inflammatory cell types were detected between the two groups. These results indicate that the major consequence of tannin consumption in goats is a reduction in worm fecundity and egg output, which does not seem related to significant changes in the local mucosal response.

Abomasum↗
PubMed2003

Effects of Acacia nilotica and Acacia karoo diets on Haemonchus contortus infection in goats.

This study was carried out to determine the effects of Acacia karoo and Acacia nilotica diets on Haemonchus contortus infections in goats. Twenty-four Boer goats of mixed sex (live weight 17-22 kg) were randomly assigned to four treatment groups, namely: A. nilotica (AN) group, A. karoo (AK) group, control infected with H. contortus (HC) group and the non-infected control (NHC) group. Animals in the AN, AK and HC groups were orally infected with a single dose of 3000 HC third stage larvae. The AN and AK groups had dried leaves of AN and AK, respectively, included in their basal diet at a rate of 40% dry matter (DM) while the HC and NHC groups had the basal diet throughout the experiment. All animals received a total feed allowance of 500 g DM per day and Katambora Rhodes grass hay ad libitum for roughage. Parameters measured included faecal egg counts and live weight. At the end of the experiment, three animals from each group were slaughtered and abomasal worm burdens were determined. A significant decrease in the faecal egg counts was recorded in animals in the AK group (P<0.05) relative to those in the AN and HC groups. The worm burdens were reduced by 34% in the AK group (P<0.05) and by 10% in the AN group (non-significant) relative to the infected control group. The study indicates that the difference in the effects of the two forages on HC infections may be related to type and concentration of tannins.

Acacia↗
PubMed2003

The anthelmintic efficacy of the plant, Albizia anthelmintica, against the nematode parasites Haemonchus contortus of sheep and Heligmosomoides polygyrus of mice.

Albizia anthelmintica Brong., belongs to the plant family Mimosaceae. The plant is widely used in East Africa by poor smallholder farmers and pastoralists to treat their livestock against internal parasites. The anthelmintic effects of water extracts from the bark of A. anthelmintica, obtained from three different geographic areas in Kenya and using different methods of preparation, were tested at different doses in sheep and mice infected with the nematode parasites Haemonchus contortus and Heligmosomoides polygyrus, respectively. Lambs were infected with 3000 infective larvae of H. contortus and treated with the plant preparations 28 days later, while mice were infected with 200 infective larvae of H. polygyrus and treated 18 days later. Proximate analysis established high levels of crude proteins in A. anthelmintica bark. Two sheep out of the 45 treated with the plant preparations suffered from transient bloat, which was relieved by dosing with a surfactant. Significant reductions in faecal egg counts were observed in lambs treated with A. anthelmintica in two of the three experiments undertaken, but the efficacy levels achieved were well below the 70% reduction required. Similar values of packed red cell volume and live weight gain were observed for treated and control lambs. There was no overall significant effect of treatment with A. anthelmintica on faecal egg and total worm counts in mice. A dose rate of 1000 mg/kg bodyweight of A. anthelmintica preparation resulted in death of all mice. The results show that A. anthelmintica at the doses and preparations used is not efficacious against H. contortus in sheep or against H. polygyrus in mice.

Albizzia↗
PubMed2003

Comparison of the resistance to sympatric and allopatric isolates of Haemonchus contortus of Black Belly sheep in Guadeloupe (FWI) and of INRA 401 sheep in France.

The resistance of a tropical breed of sheep towards the nematode parasite Haemonchus contortus (Hc) was compared to that of a European breed after artificial infection with the parasite following a 2 x 2 x 2 experimental design: two isolates (Hc Gua from Guadeloupe versus Hc Fra from France), two breeds each reared in their native habitats (Black Belly (BB) in Guadeloupe, FWI, INRA 401 reared in France), and two groups of lambs with differing infection status (one naive, i.e. infected for the first time during the experiment, and one primed, i.e. which had previously received doses of 5000 L3 on Days -35 and -32 before the infection during this experiment). Both groups were composed of 10 male lambs, which were 20 weeks of age when they were given a dose of 10,000 L3 of the relevant isolate on Day 0. Resistance was assessed by regular fecal egg counts (FECs) and blood samples, and worm counts were processed on Day 35 p.i. The FEC was lower in the BB than in INRA 401 lambs, lower in the primed than naive groups, and lower in Hc Fra than in Hc Gua. The eosinophil count remained unchanged in the naive INRA 401 groups, but increased in all the other groups. The hematocrit fell after infection, but the live weight was not affected by the infection. Worm burdens were 1868 and 5237 in the naive BB and INRA 401 groups, respectively, and 596 and 4835 in the primed BB and INRA 401 groups. The daily production of eggs per Hc Gua female worm was 1.5-fold that of Hc Fra. The number of worms had no effect on worm length or Hc in utero egg count in the BB sheep, but a positive and significant correlation between these two traits was seen in the INRA 401 lambs. These findings are indicative of innate resistance, and confirmed the high acquired resistance of the BB sheep to both sympatric and allopatric isolates of Hc. The regulation of worm population and fecundity differed in the BB and INRA 401 sheep.

Animals↗
PubMed1996

Bovine humoral immune responses to Haemonchus placei excretory secretory antigens.

The immune response of calves against excretory secretory (ES) products of adult Haemonchus placei was studied. The ES products of adult H. placei comprise at least ten polypeptides with molecular weights ranging from 10 to over 100 kDa. A primary infection of calves with H. placei did not result in a marked elevation of IgG serum antibodies reactive to ES antigens as demonstrated by ELISA. Immunoblotting experiments revealed that sera of primary infected calves recognised a 50 kDa antigen in most cases. An increase in the level of serum IgG antibodies was observed after trickle infections. The secondary immune response coincided with the recognition of a group of three ES antigens of around 50-55 kDa and one protein with a molecular weight of 24 kDa.

Animals↗
PubMed1996

Adaptation to arid environment: Haemonchus longistipes in dromedaries of Saharo-Sahelian areas of Mauritania.

The adaptations of the trichostrongylid nematode Haemonchus longistipes of dromedaries (Camelus dromedarius) to the harsh environment of Saharo-Sahelian climate were assessed by means of (i) an epidemiological survey of dromedary infection in the south-west of Mauritania, (ii) an estimate over a 3 year period of parasite distribution within the host population and of prolificacy of H. longistipes females recovered from natural populations of infected dromedaries sampled at Nouakchott's slaughterhouse, and (iii) experimental infections of young dromedaries during three different periods of the year (end of the rainy season, middle and end of the dry season). Egg excretions (estimated by faecal egg counts), infective larvae derived from eggs as well as female prolificacy showed a marked seasonal pattern: high values in the rainy season and very low values in the dry season (especially March and April). Female prolificacy differed slightly between morphotypes: the knobbed type excreting over a longer period than the linguiform and smooth types. Following experimental infections in young dromedaries, arrest of larval development took place irrespective of the period. The survival strategy of H. longistipes in the dry season was based only on arrested larval development. Patent infections occurred from July to October, i.e. during the rainy season and was facilitated by the conjunction of high prevalence and intensity of adult worm burdens associated with high female prolificacy.

Adaptation, Physiological↗
PubMed1997

Genetic resistance of three genotypes of goats to experimental infection with Haemonchus contortus.

A total of 46 weaned kids of three genotypes aged about 4-5 months were used to evaluate the effects of trickle infection with a sheep strain of Haemonchus contortus. A completely randomized 3 x 2 factorial design was used. Factors were genotype (Thai native (TN), 75% TN x 25% Anglo-Nubian (AN) and 50% TN x 50% AN) and parasite (control and infected). The animals were infected with 750 infective larvae (L3) of H. contortus three times a week for 3 weeks, with a total of 6750 larvae. The experiment lasted 9 weeks. Each week animals were weighed, faecal egg counts done and blood examined for haematological and biochemical variables. Twenty-seven kids were slaughtered at the end of experiment for worm recovery. Weight gain of infected animals was lower than those of uninfected controls (P < 0.05). The genotype 50% TN x 50% AN had higher growth rate than TN and 75% TN x 25% AN genotypes (P < 0.05). Eggs per gram of faeces (EPG) were significantly higher in 50% TN x 50% AN kids than in TN (P < 0.0005) and 75% TN x 25% AN (P < 0.0001) kids. There was a large variation in the EPG of individual animals within a genotype. The percent establishment of L3 was 8.2% in TN, 16.97% in 50% TN x 50% AN and 17.91% in 75% TN x 25% AN kids. TN kids had worm counts lower than 50% TN x 50% AN (P < 0.05) and 75% TN x 25% AN (P = 0.07) kids. Infection had a significant effect on packed cell volume (PCV), haemoglobin, total protein and albumin. The decrease in the level of these blood parameters was less in TN kids than in 50% TN x 50% AN and 75% TN x 25% AN kids. There was no significant difference between genotypes in the values of total and differential leucocyte counts and mean corpuscular volume (MCV). It can be concluded that TN goats are more resistant to H. contortus than 50% TN x 50% AN goats. However, under the present experimental conditions, the liveweight gain of 50% TN x 50% AN was higher than the TN goats.

Animals↗
PubMed1997

The in vitro uptake and incorporation of hemoglobin by adult Haemonchus-contortus.

The incorporation of radioactivity from [3H]leucine-labeled hemoglobin (Hb) into adult Haemonchus contortus proteins was investigated. Further, the role of previously described cysteine proteases present in intestinal tissue and excretory/secretory products of H. contortus was assessed in the breakdown of Hb. A cell lysate preparation (predominantly Hb) was obtained from reticulocytes metabolically labeled, in vitro, with [3H]leucine. Following 24-h incubation in the presence of [3H]Hb, adult H. contortus incorporated radioactivity. The presence of the protein synthesis inhibitor puromycin (200 micrograms ml-1) reduced incorporation by 72%, indicating that this process was dependent on protein synthesis. The specific cysteine protease inhibitor Z-phe-ala-FMK (PAF) at 0.1 mM had no effect on incorporation of radioactivity; however, the breakdown of Hbg in the culture medium was reduced by 50%. In contrast, PAF at 1.0 mM caused a 78% reduction in incorporated radioactivity. Parasite viability was also decreased by 1.0 mM PAF, and thus the reduction of incorporation of radioactivity may not be due to specific enzyme inhibition. The serine protease inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) at 1.0 mM caused a 40% reduction in incorporation of radioactivity; the aspartic protease inhibitor pepstatin (1 mM) was without effect. Adult H. contortus also incorporated radioactivity from [3H]leucine-labeled intact reticulocytes. This incorporation was inhibited-by 1.0 mM PAF and AEBSF in a manner similar to that for the cell lysate preparation. These data indicate that adult H. contortus degrade Hbg and incorporate the radioactivity into their macromolecules. The specific action of the endogenous cysteine protease in the digestion of Hbg could not be demonstrated unequivocally. However, the hypothesis that the secreted cysteine protease functions in extracorporeal digestion was supported.

Animals↗
PubMed1997

How long before resistance makes it impossible to control some field strains of Haemonchus contortus in South Africa with any of the modern anthelmintics?

This paper describes an exceptional spectrum of multiple anthelmintic resistance in two strains of Haemonchus contortus in South Africa, one from Howick in KwaZulu-Natal, and the other from Badplaas, in Mpumalanga. Apparently for the first time, a helminth strain is described with resistance to compounds from all five of the modern anthelmintic groups used for nematode control in sheep; also, two strains of H. contortus show resistance to the two substituted phenols, dinitrophenol and nitroxynil. Only closantel at 5 mg kg-1 of nine compounds tested appears to have undiminished efficacy against the Howick strain, but even in the case of closantel, the residual activity and minimal effective level need to be tested before it can be concluded that its efficiency is unaffected. The exceptional resistance of the Howick strain is demonstrated by the fact that sequential daily drenching of sheep infected with the strain, with levamisole at 18 mg kg-1, oxfendazole at 20 mg kg-1, levamisole at 20 mg kg-1 and a mixture of fenbendazole at 10 mg kg-1 plus trichlorfon at 132 mg kg-1 on the fourth day, failed to clear sheep of the infection. There are strong indications that side-resistance occurs between dinitrophenol and nitroxynil, on the one hand, and the salicylanilides, on the other, and it is suggested that, before long, strains of H. contortus will be found with high levels of resistance to all the currently available anthelmintics.

Animals↗
PubMed1997

Efficacy of closantel, albendazole and levamisole on an ivermectin resistant strain of Haemonchus contortus in sheep.

Following evidence of reduced efficacy of oral ivermectin in a field population of Haemonchus contortus on a sheep farm in Kenya, this strain of the parasite was submitted to a controlled anthelmintic test. One hundred and twenty worm-free lambs were randomly split into two groups of 60. Each lamb in the first group was infected with 5000 third stage larvae (L3) of the suspected resistant strain the remaining 60 lambs were each infected with 5000 (L3) of a H. contortus strain of known susceptibility to the major groups of anthelmintic compounds used in sheep. On day 28 post-infection, each group was subdivided according to egg counts and at random into six sub-groups of ten lambs, each of which was treated with closantel at 5.0 mg kg-1, albendazole at 5.0 mg kg-1, levamisole at 7.5 mg kg-1, oral ivermectin at 0.2 mg kg-1 or injectable (Inj.) ivermectin at 0.2 mg kg-1, or was left as an untreated control. At slaughter, 10 days later, all the anthelmintics had resulted in reduced worm burdens in animals infected with the susceptible strain (albendazole 99.0%, levamisole 99.5%, closantel, Inj. and oral ivermectin 100%). By contrast, in the lambs infected with the suspected resistant strain, closantel was 100% effective, but inj. ivermectin, oral ivermectin, albendazole and levamisole reduced worm counts by 47.6%, 24.2%, 38.5% and 41.4%, respectively. Anthelmintic resistance to the other chemical groups had been confirmed previously on this farm and although benzimidazoles and levamisole had not been used for the last 4 years, this strain was still highly resistant to albendazole and levamisole; closantel remained as the only drug evaluated which was still effective against this population of H. contortus.

Administration, Oral↗
PubMed1998

Epidemiology of gastrointestinal nematode parasitism in Suffolk and Gulf Coast Native sheep with special emphasis on relative susceptibility to Haemonchus contortus infection.

An eight-year study was conducted to define the epidemiology of gastrointestinal nematode infection in Suffolk and Gulf Coast Native (Native) breeds of sheep, and to determine if the Native sheep is more resistant to infection. For the initial three years, each breed grazed separate pastures where anthelmintic treatments were administered to individual animals on a salvage basis. For the last five years, both breeds grazed concurrently; anthelmintic treatments were administered to individual animals on a salvage basis for the first three years, and to all animals, when treatment criteria were met, for the last two years. The fecal egg count (FEC) and blood packed cell volume (PCV) were monitored, and tracer lamb nematode burdens were determined. Overall, FEC for both breeds increased in the spring (periparturient rise) for most years and in the summer for all years. Under separate grazing conditions, Native ewes and lambs had consistently lower infection levels than Suffolk ewes and lambs. During the haemonchosis season (June-September) each year, Suffolk ewe and lamb PCV decreased, and Native ewe and lamb PCV remained relatively stable. The salvage treatment protocol resulted in 27 treatments for Suffolk and one for Native ewes; similarly for lambs, 13 for Suffolk and zero for Native. Tracer lambs grazed with their respective breed, and the FEC and mean total nematode burden corresponded with the pattern of infection for their respective breed. The predominant nematodes found in Suffolk and Native tracer lambs were Haemonchus contortus and Trichostrongylus spp., respectively. Under concurrent grazing conditions, the same seasonal repeatable pattern of infection was present and was exhibited by both breeds, with the Native ewes and lambs being consistently and significantly (p < or = 0.05) lower for FEC and higher for PCV. The salvage treatment protocol resulted in 57 and zero treatments for Suffolk and Native ewes, respectively; for lambs, 46 and 11. Tracer lamb nematode burdens again corresponded to their respective breed pattern of infection, with H. contortus and Trichostrongylus spp. being predominant in Suffolk and Native lambs, respectively. Data from all tracer lambs showed a relatively low level of hypobiosis (H. contortus only), and, although there was no consistent hypobiosis season, the tendency was for a higher level to occur in the fall. These results showed that the classic repeatable seasonal pattern of gastrointestinal nematode infection occurred in both breeds of sheep, and that Native sheep were more resistant to infection (specifically H. contortus) than Suffolk sheep.

Animal Feed↗
PubMed1997

The in vitro uptake of albumin by adult Haemonchus contortus is altered by extracorporeal digestion.

FITC (fluorescein isothiocyanate)-labeled albumin was used as a substrate to further characterize the digestion of host blood proteins by adult Haemonchus contortus. Isolated H. contortus intestine degraded FITC-albumin; degradation was completely inhibited by E-64, a specific inhibitor of cysteine proteases. The in vitro uptake and degradation of FITC-albumin by parasites was also evaluated. Uptake of fluorescence was demonstrated; greater than 50% of the fluorescence was associated with degraded FITC-albumin. However, both the uptake and degradation of FITC-albumin were reduced by about 85%. Additionally, when parasites were incubated in the presence of specific inhibitors of cysteine proteases, degradation of FITC-albumin was shown to occur rapidly in the media during in vitro incubation. This degradation was blocked completely by the presence of cysteine protease inhibitors. These results indicate that the rapid extracorporeal digestion of FITC-albumin (mediated by secreted cysteine proteases) produce relatively high concentrations of low molecular weight FITC-labeled fragments and alters the kinetics of uptake of fluorescence by the parasite. These fragments rather than FITC-albumin are rapidly taken up by the parasites. Thus in vitro demonstration of a definitive role for the cysteine proteases as intestinal digestive enzymes in H. contortus is compromised by the enzyme's extracorporeal activity. This extracorporeal cysteine protease activity may represent the enzyme's predominant activity.

Abomasum↗
PubMed1998

Response to challenge infection with Haemonchus contortus and Trichostrongylus colubriformis in dairy goats. Consequences on milk production.

The objective of the study was to examine the ability of dairy goats to develop a response to nematode parasites of the digestive tract after a previous contact with the worms. One hundred dairy goats were initially divided into 2 groups. One remained free of parasites (not previously infected: NPI). The second group was infected thrice at 50 days interval with a mixture of Haemonchus contortus and Trichostrongylus colubriformis (previously infected: PI). After this initial exposure to nematodes, the goats were drenched with fenbendazole to eliminate the worms and remained free of parasites for 2 months around kidding. One month after kidding, 24 lactating dairy goats from each initial group were challenged with a mixture of H. contortus and T. colubriformis and parasitological, pathophysiological and milk production parameters were measured fortnightly to assess the effects of challenge infection and the response of the goats. No difference in parasite egg excretion was found between group NPI and PI after challenge infection. Similarly, no difference in worm counts was detected in 5 culled goats from each group killed one month post challenge. In contrast, differences between both groups were detected when considering the pathophysiological parameters (packed cell volume, inorganic phosphate and pepsinogen concentrations) with consequences of infection being more severe in the previously infected animals. Moreover, the milk production was also depressed in the PI group when compared to the NPI one. These results indicate that the response developed by adult dairy goats after the challenge infection was unable to limit the worm populations but could have pathophysiological repercussions and consequences on production.

Analysis of Variance↗
PubMed1998

A hemolytic factor from Haemonchus contortus alters erythrocyte morphology.

A hemolytic factor from adult Haemonchus contortus caused distinct morphological changes in the surface of sheep red blood cells (RBCs). After a 15 min exposure to the hemolytic factor, hemolysis was not detected in incubation media, but RBCs were spherical in shape with numerous surface projections compared to control cells that were smooth-surfaced biconcave disks. After 30 min, a time at which significant hemolysis occurred, echinocytes were formed, and after 90 min, cells were severely disrupted with many visible holes in membranes. No RBC ghosts were observed. RBCs from four other mammalian species were lysed by the H. contortus hemolytic factor. However, the rate of hemolysis varied with a relative order of sheep approximately rabbit>goat>pig>calf. The morphology of RBCs from all four species was significantly altered after 30 min incubation with the degree of morphological changes related to the degree of hemolysis. These results support the hypothesis that the hemolytic factor acts as a pore-forming agent, although a phospholipase or other enzyme might play a role in solubilization of cell membranes.

Animals↗
PubMed1999

Comparison of six in vitro tests in determining benzimidazole and levamisole resistance in Haemonchus contortus and Ostertagia circumcincta of sheep.

Six in vitro methods for the detection of anthelmintic resistance were compared using benzimidazole/levamisole-resistant Haemonchus contortus and benzimidazole/levamisole/ivermectin-resistant Ostertagia circumcincta as well as susceptible strains of both parasite species. The degree of resistance to thiabendazole and levamisole was compared by (1) an egg hatch assay, (2) an egg hatch paralysis assay, (3) a larval development assay, (4) a larval paralysis assay (5) a larval paralysis assay with physostigmine and (6) larval micromotility assay. The degree of resistance for each assay was expressed as resistance factor--RF. For the detection of thiabendazole and levamisole resistance, the larval development test was observed as the most sensitive to measure quantitatively a degree of resistance between susceptible and resistant strains. For this test the RF for thiabendazole and levamisole was 14.3 and >32.5, respectively in H. contortus strains and 21.1 and 3.5 in strains of O. circumcincta. Egg hatch assay was also found to be sensitive and accurate in determining of resistance to benzimidazole. For measurement of levamisole resistance the egg hatch paralysis assay and larval paralysis assay were found to be able to distinguish between strains, but some disadvantages of these techniques make it unsuitable for field detection of levamisole resistance. Other in vitro assays as larval paralysis assay with physostigmine and larval micromotility assay were also investigated. Significant differences in paralysis of the larvae were observed using larval paralysis assay.

Animals↗
PubMed1999

Serum antibody response of Castellana sheep to Haemonchus contortus infection and challenge: relationship to abomasal worm burdens.

Primary and secondary serum antibody responses to Haemonchus contortus were studied in Castellana sheep. Ten-month-old sheep were infected (200 L3/kg live weight (lw)) and challenged (400 L3/kg lw) or uninfected and equally challenged with H. contortus. Primary infections induced a partially protective response upon challenge, characterized by higher serum protein levels, longer prepatent periods, lower fecal egg counts, and significant reduction in the establishment rate of the parasite and abomasal adult and L4 worm burdens. The resistant status of the infected and challenged sheep was not clearly related either to the serum specific antibody levels (IgG: IgG1, IgG2; IgM; IgA) estimated by ELISA or to immunodetection patterns in the Western blots.

Abomasum↗