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PubMed1997

Allergenic proteins in different brands of latex and synthetic medical examination gloves.

The steady increase in anaphylactic reactions to latex medical gloves has raised increasing awareness in the medical community. Even gloves claimed to be hypo-allergenic still may contain substantial amounts of IgE-binding proteins. We have studied non-powdered latex and synthetic examination glove extracts for their allergenicity using the immunoblot technique. Protein levels varied considerably among glove extracts and the amount did not always correlate with the presence of allergenic proteins. IgE binding proteins were found in 2 of the 7 powder-free latex glove brands. Synthetic glove extracts did not contain allergens. The study demonstrates that immunoblot analysis is a useful technique in order to select gloves with a minimal risk to raise IgE antibodies.

Allergens↗

PubMed1994

The effect of surgical glove powder on proliferation of human skin fibroblast and monocyte/macrophage.

The effect of surgical glove powders (Biosorb, Keoflo, and CaCO3) and Hydrocote (powder-free lubricating agent, Biogel) was examined on human skin fibroblasts and monocyte/macrophage cell lines (U937 and HL-60). Glove powders (0.1-100 micrograms/ml) in the presence of 10% fetal bovine serum (FBS) had no significant effect on the rate of 3H-thymidine uptake and proliferation of these cells after 48 h and 7 days of exposure, respectively. However, they inhibited HL-60 growth after 10 days, and Biosorb and CaCO3 inhibited U937 after 10-21 days of exposure compared with control. In the presence of low serum (0.5%), Biosorb, but not Keoflo, CaCO3, and Hydrocote, inhibited HL-60 cells after the third day of exposure (p < 0.05), whereas they were without any effect on U937 cells. Further incubation resulted in a significant decrease in cell density in all treatments, as well as controls, because of cell death. In the presence of 2% serum, glove powder-treated HL-60 significantly increased in cell numbers during the first 3 days, and the cells became stationary thereafter, whereas Keoflo and CaCO3-stimulated U937 reached a maximal by 9 days of treatment. Coculturing of fibroblasts directly with macrophages (0.4-5 x 10(5) cells per dish) or incubation with macrophage culture-conditioned media (CCM) stimulated quiescent fibroblast growth equal to that induced by 10% and 0.5% serum, respectively (p < 0.05). However, incubation of fibroblasts with glove powder-treated HL-60 CCM (except CaCO3) inhibited (p < 0.05) and CCM from Biosorb-treated U937 stimulated (p < 0.05) fibroblast proliferation. The CCM from glove powder-treated HL-60 and U937 did not have any significant effect on the rate of 3H-thymidine incorporation into fibroblasts compared with controls. The present observations suggest that glove powder action on fibroblast and macrophage growth in vitro depends on both the serum concentration of the culture medium and the length of exposure. The results imply that glove powders may have an adverse effect in vivo by directly influencing the biologic activity of macrophages, as well as other cell types, leading to alterations in the early phases of wound healing.

Calcium Carbonate↗

PubMedDate not supplied

Use of nonsterile gloves for routine noninvasive procedures in thermally injured patients.

A total of 26 boxes of gloves were analyzed to determine if using nonsterile gloves for routine noninvasive procedures was sufficient for thermally injured patients and if the risk of infection increased. All of the study boxes had some organism present on or in the used box; the most common type found was Staphylococcus aureus. Eleven of the 13 subjects (85%) had specific antibiotic-resistant strains of S. aureus present on cultures obtained from open wounds. Seven (64%) of these corresponded to the glove boxes assigned to that patient. The remaining four boxes of gloves had no S. aureus present. In all of the boxes of gloves that had positive S. aureus cultures, 100% of the resistant strains occurred after it was first cultured from the patient. As a result, nonsterile gloves can be used safely for routine non-invasive procedures in the thermally injured patient. It is imperative to avoid using a common box of gloves for two or more patients to prevent the transfer of organisms between patients.

Bandages↗

PubMedDate not supplied

A laboratory evaluation of standard leakage tests for surgical & examination gloves.

Analysis of leakage tests for surgical and examination gloves is reported. Six widely applicable tests were evaluated, using samples from 16 glove manufacturers. Various techniques for inducing sub-millimeter holes were developed and employed for creating test holes in a wide variety of gloves. The prescribed tests were performed and the results compared to calculations of expected sensitivities. In general, there was reasonable agreement between calculation and measurement (within about a factor of 2), although there were some exceptions. Minimum detectable hole sizes, before the holes were stretched by the tests, were generally in the range of 25-100 microns in diameter. Theoretical predictions of minimum detectable hole sizes for the various tests ranged from about 100-200 microns (in the stretched condition). Because of inherent limitations of the test protocols, it is clear that much larger holes can go undetected under certain circumstances, especially in examination gloves. Some test procedures had important procedural ambiguities; some tests were patently impossible to perform. All tests had inherent limitations resulting in sensitivities that varied from glove to glove or that were quite nonuniform over the surface of the glove being tested or both.

Equipment Design↗

PubMedDate not supplied

Comparison of positive pressure gloves on hand function in adults with burns.

The purpose of this study was to analyze the impact of a standard, custom-made pressure glove vs The NewYork-Presbyterian Dexterity Glove (NYPDG) with silon application on the palmer surface on functional hand use of burn survivors. A standard, custom-made pressure glove and NYPDG were given to 18 participants in a randomized order. Subjects wore each glove for 7 to 10 days during all activities of daily living (ADL). Variables such as hand function, difficulty of fine and gross motor ADL, and participant glove preference were assessed with each glove condition. Data collection of the second glove took place 7 to 10 days later incorporating a quasiexperimental, repeated measure design. A crossover design was used to analyze the data. The NYPDG demonstrated significantly better results in all of the four outcome categories measured: time to complete the Jebsen, the Jebsen Likert scale, fine motor ADL, and gross motor ADL. This study demonstrated that functional tasks took less time to complete and were more easily performed when using the NYPDG.

Activities of Daily Living↗

PubMed2005

Surgical glove-associated diffuse lamellar keratitis.

PURPOSE: To demonstrate a potential cause of a prolonged epidemic of diffuse lamellar keratitis (DLK). METHOD: This retrospective review analyzed an epidemic of diffuse lamellar keratitis over a 3-year period in a single surgery center. Altering the brand of surgical gloves used during surgery was associated with an elimination of the DLK epidemic. Optical microscopy, scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), and Fourier transform infrared (FTIR) spectroscopy were performed on both brands of surgical gloves to allow for comparisons and determine possible surface contaminants responsible for the DLK outbreaks. RESULTS: The incidence of DLK during the epidemic ranged from 2% to 38% on a quarterly basis for the 3-year period. A change in the brand of surgical gloves resulted in a cessation of DLK. Surface analysis of both brands of gloves revealed extensive silicone oil contamination on the internal and external surfaces of the DLK-associated gloves and insignificant amounts of silicone oil on the external surface of the DLK-free gloves. CONCLUSION: Silicone oil contamination of surgical gloves appears to be associated with epidemic DLK.

Corneal Stroma↗

PubMed2006

Formaldehyde in reusable protective gloves.

Due to the clinical findings in a single patient's case, formaldehyde was suspected to be present in clinically relevant levels in reusable protective gloves. Therefore, 9 types of gloves were investigated with the semi-quantitative chromotropic acid method. It was found that 6/9 gloves emitted some formaldehyde and that 4/9 gloves emitted > or =40 microg of formaldehyde. Most of the formaldehyde was found on the inside of the gloves. To get an indication of the clinical relevance, a comparison with a protective cream declared to contain the formaldehyde-releasing agent diazolidinyl urea was performed by comparing areas of gloves with areas of cream layers with thickness 1-2 mg/cm(2). It was found that the amounts of formaldehyde emitted from the gloves might be in the same range as emitted from a layer of cream.

Adult↗

PubMed1989

Capacitance effect of rubber gloves on electric pulp testers.

Electric pulp testers (EPTs) are widely used to assess tooth pulp vitality. With many unipolar EPTs the electrical circuit is completed through the operator. Since dentists now routinely wear rubber gloves, these might be expected to provide electrical insulation, and therefore to break the circuit. The objective of this investigation was to define the electrical effect of wearing rubber gloves. Two battery-powered unipolar EPTs were examined using a digital storage oscilloscope with an input impedance of 1 M omega. The probe tip was connected directly to the positive input lead of the oscilloscope. Three conditions were tested: (i) with the conductive handle connected directly to the negative input lead; (ii) with the ungloved operator holding the negative input lead in one hand and the conductive handle of the EPT in the other; and (iii) holding the conductive handle in a gloved hand. While the two EPTs produced different patterns of voltage spike, for each there was no difference between conditions (i) and (ii). However, when the conductive handle was held in a gloved hand (condition iii), there was a reduction in peak negative voltage and a change in wave form with positive overshoot. The alteration in wave form could be reproduced by substitution of the operator's gloved hand with a capacitance of 47 pF. These results support the hypothesis that a rubber glove acts partly as a capacitor in series with the electric pulp tester, and will alter the performance of EPTs unless the glove is bypassed electrically.

Dental Pulp Test↗

PubMed1992

Effectiveness of handwashing agents in eliminating Staphylococcus aureus from gloved hands.

The recent emphasis on gloving has resulted in accounts of healthcare workers washing gloves instead of changing them. This study evaluated the efficacy of soap and three germicidal agents in decontaminating latex and vinyl medical gloves that were experimentally contaminated with Staphylococcus aureus. Gloves were tested for perforations before and after the glove-washing procedure. Results of this study demonstrated that contamination was reduced from the glove surfaces. Although the routine washing of gloved hands cannot be recommended, it may be carried out under limited circumstances.

Disinfectants↗

PubMed1995

A comparative study of two types of latex surgical gloves in elective orthopaedic surgery.

Glove perforation during surgery represents a potential risk of infection for the surgeon. The authors postulated that thicker latex gloves may offer greater protection. The perforation rates for the Ansell Gammex glove and the thicker Baxter Triflex gloves were compared in single- and double-glove usage and no difference was found between the two brands, in either usage. The authors recommend double-gloving for all orthopaedic operations with regular glove changes during major procedures.

Equipment Failure↗

PubMed1999

The integrity of surgical gloves during gynaecological operations.

The study was designed to evaluate the integrity of all surgical gloves, worn as single-gloving, used in 100 consecutive gynaecological operations. Glove perforations were tested by a standardized hydrosufflation technique. Of the 326 pairs of gloves used, 46 were found to have perforations (an overall rate of 7.1%) and about 30% had more than 1 perforation. The rate of glove perforation was 19% among charge nursing sisters, 16% among surgeons and 13% among first assistants--these differences were not statistically significant. The duration of the operation and degree of operative difficulty strongly influenced the rate of perforation. We recommend that surgeons, charge sisters and first assistants should change their gloves after every hour of the operation and use double-gloving in anticipation of a difficult operation.

Equipment Failure↗

PubMed1994

Glove-related skin symptoms among operating theatre and dental care unit personnel (I). Interview investigation.

233 employees in hospital and dental care participated in an interview investigation on glove-related skin complaints. 37% (87/233) reported skin symptoms related to glove use. 2% (4/233) reported localized contact urticaria provoked by latex gloves, 10% (23/233) hand eczema and 24% (56/233) unclassifiable skin intolerance reactions from gloves. Another 2% (4/233) reported facial irritation from gloves. Glove-related skin symptoms were thus reported by more than 1/3 of the personnel and the reported glove-provoked contact urticaria constituted 5% (4/87) of the intolerance reactions.

Adolescent↗

PubMed1996

Effect of glove occlusion on human skin. (I). short-term experimental exposure.

UNLABELLED: Skin barrier function was studied after use of occlusive gloves on normal and compromised skin. 2 studies were performed (Study A and B), and the effects were evaluated by non-invasive methods. Participants in the studies were instructed to wear an occlusive glove on one hand, while the other hand served as control. The gloves used were hypoallergenic, non-latex. Study A: 20 volunteers wore a glove on normal skin 6 h/day for 3 days. Study B: 20 volunteers wore a glove on sodium lauryl sulfate(SLS)-compromised skin 6 h/day for 3 days. Skin barrier function was evaluated by measurement of transepidermal water loss (TEWL) (Evaporimeter), skin hydration by electrical capacitance (Corneometer) and inflammation was evaluated by erythema index (DermaSpectrometer). RESULTS: Study A. Glove occlusion on normal skin 6 h/day for 3 days caused no significant influence on the water barrier function. Study B: Glove occlusion on SLS-compromised skin for the same period of time had a significantly negative effect on the water barrier function. It is concluded that occlusion may be an additional factor in the pathogenesis of cumulative irritant contact dermatitis.

Adult↗

PubMed2006

High school and college baseball pitchers response and glove movements to line drives.

The timing of glove movements used by baseball pitchers to catch fast approaching balls (i.e., line drives) was examined in two tests to determine the responses and temporal characteristics of glove movements in high school and college baseball pitchers. Balls were projected toward the head of participants at 34.8 m.s-1 (78 mph) on average in an indoor test and at speeds approaching 58.1 m.s-1 (130 mph) in a field test. Pitchers caught over 80% and 15% of the projected balls in the indoor and field tests, respectively. Analyses of glove responses indicated that all pitchers could track the line drives and produce coordinated glove movements, which were initiated 160 ms (+/-47.8), on average, after the ball was launched. College pitchers made initial glove movements sooner than high school pitchers in the field test (p=0.012). In contrast, average glove velocity for pitchers increased from 1.33 (+/-0.61) to 3.45 (+/-0.86) m.s-1 across the tests, but did not differ between experience levels. Glove movement initiation and speed were unrelated, and pitchers utilized visual information throughout the ball's flight to catch balls that approached at speeds exceeding the estimated speeds in competitive situations.

Adolescent↗

PubMed2002

PCR-based method for detecting viral penetration of medical exam gloves.

The test approved by the U.S. Food and Drug Administration for assessment of the barrier quality of medical exam gloves includes visual inspection and a water leak test. Neither method tests directly the ability of gloves to prevent penetration by microorganisms. Methods that use microorganisms (viruses and bacteria) to test gloves have been developed but require classical culturing of the organism to detect it. We have developed a PCR assay for bacteriophage phiX174 that allows the rapid detection of penetration of gloves by this virus. The method is suitable for use with both latex and synthetic gloves. The presence of glove powder on either latex or synthetic gloves had no effect on the ability of the PCR assay to detect bacteriophage DNA. The assay is rapid, sensitive, and inexpensive; requires only small sample volumes; and can be automated.

Bacteriophage phi X 174↗

PubMed2004

Non-touch suturing technique fails to reduce glove puncture rates in an accident and emergency department.

OBJECTIVE: To assess the impact of introducing a safer non-touch suturing technique into an inner city emergency department. METHODS: The rate of glove perforation, measured by electrical conductance, was used as a marker. Gloves (Bodyguards) used in suturing were collected over a two month period. Two half day suture workshops were then conducted in the emergency department and gloves were collected for a further two months. All doctors (14) were included in the study regardless of grade. Gloves were tested for perforation by electrical conductance. RESULTS: There were 107 gloves and 19 perforations in the first group, 133 with 28 perforations in the second (p = 0.52), and one perforation in 100 control gloves. Most doctors reported inadvertent needlestick handling. CONCLUSION: Two half day suturing workshops are not enough to reduce glove perforations from suturing in the emergency department. Difficulty with compliance suggests that safe suturing practice must be taught before doctors develop bad habits that are difficult to change.

Education, Medical, Continuing↗

PubMed1992

Frequency of glove puncture in the post mortem room.

The post mortem room is a potential source of danger from contamination caused by glove punctures. The objective of this study was to assess the incidence of hand injury and hand contamination during post mortem examinations. A prospective study of injury and glove puncture rate was conducted during post mortem examinations performed in 1990 on adults in Northwick Park Hospital mortuary. Four pathologists and two assistants performed a total of 44 post mortem examinations on adults. All participants completed a questionnaire after each examination, recording the total number of gloves used and the number of hand injuries and glove perforations sustained. Just over 8.3% of gloves were punctured; 31.8% of these punctures went unnoticed. Evisceration was the procedure most likely to result in hand contamination. The study highlights a significant risk which could be reduced by more care, frequent glove changes, and hand washing during post mortem examinations. In particular, gloves should be changed and hand washed when evisceration has been completed.

Autopsy↗

PubMed2004

Evaluation of the protective effectiveness of gloves from occupational exposure to 2-methoxyethanol using the biomarkers of 2-methoxyacetic acid levels in the urine and plasma.

AIMS: To evaluate the protective effectiveness of gloves from occupational exposure to 2-methoxyethanol (2-ME); and to examine the association of 2-methoxyacetic acid (MAA) in urine and plasma collected simultaneously from low 2-ME exposure and high 2-ME exposure workers in a semiconductor copper laminate circuit board manufacturing plant. METHODS: Eight hour time weighted breathing zone monitoring was performed to verify the 2-ME exposure classification between workers in regular and special operations. Urine and plasma samples were simultaneously collected from 74 exposed and 80 non-exposed workers. MAA concentrations in the urine (UMAA) and plasma (PMAA) were measured using previously published methods. Three types of gloves worn by workers (cotton, rubber, and no gloves) were recorded by direct observations in the workplace and validated by person-to-person interview. Protective effectiveness indices (PEI) were used to evaluate the glove effectiveness. RESULTS: There was no detectable 2-ME/MAA in the air, or in urine and plasma samples in non-exposed workers. The average UMAA and PMAA in special operations were 72.63 mg/g Cr. and 29.72 mg/l, significantly higher than values in regular operations (5.44 mg/g Cr. and 2.58 mg/l, respectively). PMAA showed satisfactory correlation to UMAA in all participants from both regular and special operations. The rubber gloves provided significant reduction in 2-ME uptake, whereas cotton gloves provided little protection with fluctuating effectiveness, based on PEI estimates. CONCLUSIONS: PMAA, similar to UMAA, could serve as a specific biomarker for 2-ME exposure. Wearing impermeable rubber gloves during high risk tasks can reduce major 2-ME exposure. Other improvements, including engineering control, should be provided to diminish worker exposure to 2-ME in occupational environments.

Acetates↗