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Flea (Siphonaptera:Ceratophyllidae, hystrichopsyllidae) and tick (Acarina:Ixodidae) control on wood rats using host-targeted liquid permethrin in bait tubes.

The efficacy of a liquid permethrin-treated bait tube controlling fleas and ticks on Mexican wood rats, Neotoma mexicana Baird, was evaluated during a 1-yr study in north-central Colorado. Results indicated that the bait tubes were effective for reducing flea and tick infestations on wood rats. The effects of treatment persisted throughout the study, despite the fact that bait tubes were replenished with bait and permethrin only during the first 4 mo (4 replenishments). Our results suggest that these bait tubes provide an effective, economical, and environmentally acceptable means of controlling vectors of flea or tick-borne diseases, although slight modifications of the basic bait tube design might be required to maintain the effectiveness of the tube under different ecological conditions.

Animals↗

Fleas in French Guiana (Insecta: Siphonaptera).

Based on a collection of approximately 760 fleas, of which 520 were collected in 1994-1995 during scientific studies at the Petit Saut dam site in French Guiana, 12 species and subspecies are discussed. Two taxa, Rhopalopsyllus garbei Guimarães, 1940 and Adoratopsylla intermedia copha (Jordan, 1926), are new records for French Guiana. The fleas collected during the 1994-1995 study were obtained from 35 species of mammals (3,484 specimens); of which, 21 (60%) species were negative for fleas. The mammals were captured primarily by live-trapping during a capture-release study along a portion of the Sinnamary River and its tributaries up to a distance of 70 km upstream from the Petit Saut dam site.

Animals↗

Bayesian population dynamics of interacting species: great gerbils and fleas in Kazakhstan.

We propose a discrete-time Bayesian hierarchical model for the population dynamics of the great gerbil-flea ecological system. The model accounts for the sampling variability arising from data originally collected for other purposes. The prior for the unknown population densities incorporates specific biological hypotheses regarding the interacting dynamics of the two species, as well as their life cycles, where density-dependent effects are included. Posterior estimates are obtained via Markov chain Monte Carlo. The variance of the observed density estimates is a quadratic function of the unknown density. Our study indicates the presence of a density-dependent growth rate for the gerbil population. For the flea population there is clear evidence of density-dependent over-summer net growth, which is dependent on the flea-to-gerbil ratio at the beginning of the reproductive summer. Over-winter net growth is favored by high density. We estimate that on average 35% of the gerbil population survives the winter. Our study shows that hierarchical Bayesian models can be useful in extracting ecobiological information from observational data.

Animals↗

Indicators of sexual maturation and regression in the female cat flea, Ctenocephalides felis.

Differences in the salivary glands, mesenteron epithelium and reproductive organs of female cat fleas, Ctenocephalides felis Bouché (Siphonaptera: Pulicidae), are related to the degree of reproductive maturation or regression. Contrary to previous ideas, blue bodies in the ovarioles are degenerate oocyte nuclei and their presence denotes failure of ripening oocytes to reach full maturity. A distinction between true corpora lutea and pseudo-corpora lutea is established, the presence of the former indicates successful oviposition, and of the latter, failure to complete maturation of eggs. Accurate indicators of sexual maturation and reproductive success are of potential value in assessing relative suitability of various hosts for a given flea species and therefore in assessing the degree of host specificity among fleas.

Animals↗

Some toxicity aspects of dichlorvos flea collars in cats.

The effects of the application of a single dichlorvos flea collar containing 500 mg of the insecticide were observed in 23 growing cats for periods of from 1 to 15 weeks. No overt signs of toxicity occurred and weight gain in young growing animals was not significantly affected. Whole blood cholinesterase was reduced within the first week of application of the collar and remained below pretreatment levels for 5 to 8 weeks before returning to normal presumably as the insecticide concentration in the resin was progressively reduced. Signs of systemic organophosphate toxicity did occur within 1 week of the application of three collars to each of two cats kept in the same cage. This suggested that the likelihood of toxicity occurring in cats following the use of dichlorvos flea collars depended mainly on the degree of exposure to the insecticide. Considering recent reports in the literature it was proposed that the environmental conditions of temperature (11 degrees to 23 degrees C) and relative humidity (50 to 65%) under which the experiments were carried out would have promoted a relatively low atmospheric concentration of dichlorvos in the near environment of the test animals and hence generally prevented occurrence of clinical signs. Under most conditions of keeping domestic cats in Australia the use of a single dischlorvos flea collar should therefore be without any untoward effect although some initial and transient inhibition of whole blood and nervous tissue cholinesterases would be expected.

Animals↗

Role of the Yersinia pestis hemin storage (hms) locus in the transmission of plague by fleas.

Yersinia pestis, the cause of bubonic plague, is transmitted by the bites of infected fleas. Biological transmission of plague depends on blockage of the foregut of the flea by a mass of plague bacilli. Blockage was found to be dependent on the hemin storage (hms) locus. Yersinia pestis hms mutants established long-term infection of the flea's midgut but failed to colonize the proventriculus, the site in the foregut where blockage normally develops. Thus, the hms locus markedly alters the course of Y. pestis infection in its insect vector, leading to a change in blood-feeding behavior and to efficient transmission of plague.

Animals↗

Identification of gmhA, a Yersinia pestis gene required for flea blockage, by using a Caenorhabditis elegans biofilm system.

Yersinia pestis, the cause of bubonic plague, blocks feeding by its vector, the flea. Recent evidence indicates that blockage is mediated by an in vivo biofilm. Y. pestis and the closely related Yersinia pseudotuberculosis also make biofilms on the cuticle of the nematode Caenorhabditis elegans, which block this laboratory animal's feeding. Random screening of Y. pseudotuberculosis transposon insertion mutants with a C. elegans biofilm assay identified gmhA as a gene required for normal biofilms. gmhA encodes phosphoheptose isomerase, an enzyme required for synthesis of heptose, a conserved component of lipopolysaccharide and lipooligosaccharide. A Y. pestis gmhA mutant was constructed and was severely defective for C. elegans biofilm formation and for flea blockage but only moderately defective in an in vitro biofilm assay. These results validate use of the C. elegans biofilm system to identify genes and pathways involved in Y. pestis flea blockage.

Animals↗

Characterization of spotted fever group rickettsiae in flea and tick specimens from northern Peru.

Evidence of spotted fever group (SFG) rickettsiae was obtained from flea pools and individual ticks collected at three sites in northwestern Peru within the focus of an outbreak of febrile disease in humans attributed, in part, to SFG rickettsia infections. Molecular identification of the etiologic agents from these samples was determined after partial sequencing of the 17-kDa common antigen gene (htrA) as well as pairwise nucleotide sequence homology with one or more of the following genes: gltA, ompA, and ompB. Amplification and sequencing of portions of the htrA and ompA genes in pooled samples (2 of 59) taken from fleas identified the pathogen Rickettsia felis. Four tick samples yielded molecular evidence of SFG rickettsiae. Fragments of the ompA (540-bp) and ompB (2,484-bp) genes were amplified from a single Amblyomma maculatum tick (tick 124) and an Ixodes boliviensis tick (tick 163). The phylogenetic relationships between the rickettsiae in these samples and other rickettsiae were determined after comparison of their ompB sequences by the neighbor-joining method. The dendrograms generated showed that the isolates exhibited close homology (97%) to R. aeschlimannii and R. rhipicephali. Significant bootstrap values supported clustering adjacent to this nodule of the SFG rickettsiae. While the agents identified in the flea and tick samples have not been linked to human cases in the area, these results demonstrate for the first time that at least two SFG rickettsia agents were circulating in northern Peru at the time of the outbreak. Furthermore, molecular analysis of sequences derived from the two separate species of hard ticks identified a possibly novel member of the SFG rickettsiae.

Animals↗

Evaluation of flea control strategies using fipronil on cats in a controlled simulated home environment.

Three groups of six cats were kept in similar carpeted pens in which a self-replicating population of Ctenocephalides felis had been established. One group was left untreated, but the other groups were treated every 28th day with 0.5 ml of a 10 per cent fipronil spot-on formulation, and the cats in one of the treated groups also wore a methoprene collar. No fleas were found on any of the treated cats, either during the first 13 weeks of the study, when heavy flea burdens were developing in the control pen, or over the next 11 weeks when a declining number of fleas was present on the control group.

Animals↗

[Ectoparasites in rodents of the urban region of Belo Horizonte, MG. III. Fleas, Anoplura and Acari indices in Rattus norvegicus norvegicus].

The total and specific indices of fleas, lice and mites were determined for ectoparasites on Rattus norvegicus norvegicus capture in urban areas of Belo Horizonte, Minas state, Brazil, from June 1980 to September 1982. In view of the limiting or critical values attributed to flea indices above all the "cheopis" index, proposed by several authors as a complementary measure for bubonic plague surveillance, the community of Belo Horizonte would have been exposed to this infection. The annual total indices ranged from 0.3 to 2.4 and the prevalent flea was Xenopsylla cheopis (99.2%), with the highest indices coinciding with the late dry-cool season. On two occasions, in this period, the community would have remained highly exposed to infection, since the index-limits were superseded: 8.8 (October 1980) and 6.2 (September 1982). It is suggested that preventive measures, such as protection against rat insecticide treatment may be efficiently applied in the late dry-cool season, or previous to the rainy season, before the elimination of rats. Reports on indices of lice and mites are important as they may establish index-limits for certain infections exclusive to rodents.

Animals↗

Population densities of corn flea beetle (Coleoptera: Chrysomelidae) and incidence of Stewart's wilt in sweet corn.

To quantify populations of the corn flea beetle, Chaetocnema pulicaria Melsheimer (Coleoptera: Chrysomelidae), and refine estimates of a threshold for its control to prevent Stewart's wilt caused by Erwinia stewartii, sequential plantings of 'Jubilee' sweet corn were made at 2-wk intervals from April or May through August or September 2001 and 2002 at four locations from southern to northern Illinois: Simpson, Brownstown, Champaign, and Mendota. Densities of C. pulicaria and incidence of Stewart's wilt were monitored weekly. At Mendota, where C. pulicaria populations were decimated by cold temperatures during winter 2000-2001, densities reached 33.3 beetles per 15-cm yellow sticky trap per day by September 2002, after a mild 2001-2002 winter. Maximum incidence of Stewart's wilt in single plots at Simpson, Brownstown, Champaign, and Mendota was 22, 36, 39, and 2%, respectively, in 2001, and 33, 47, 99, and 87%, respectively, in 2002. In 24 plots where beetle densities were < or =2 per trap per day, Stewart's wilt incidence was <5% in 20 plots. We propose that two corn flea beetles per trap per day be used as a threshold for insecticide application to seedlings to control C. pulicaria and minimize subsequent incidence of Stewart's wilt in processing sweet corn. Enzyme-linked immunosorbent assays indicated that E. stewartii incidence in C. pulicaria peaked at 67, 62, and 54%, respectively, at Simpson, Brownstown, and Champaign, in 2001, and at 71, 76, and 60%, respectively, in 2002. Further studies might allow the use of areawide or field-specific estimates of E. stewartii incidence in corn flea beetles for adjusting management decisions.

Animals↗

Topical application as a method for comparing the effectiveness of insecticides against cat flea (Siphonaptera: Pulicidae).

A topical (micro-droplet) bioassay is described for the cat flea, Ctenocephalides felis (Bouche). Key parameters are the short carbon dioxide anesthetization and the use of 0.1 ml of acetone per flea. The method was used to compare the effectiveness of 13 insecticides. LD95 values in nanograms per flea were nitenpyram 0.68, fipronil 0.69, deltamethrin 0.70, imidacloprid 0.81, cypermethrin 5.4, fenthion 8.0, diazinon 12, permethrin 19, malathion 29, bendiocarb 170, DDT 710, propoxur 1,300, carbaryl >10,000. Experiments repeated 2-5 yr later, with five of the chemicals, in a different facility, showed only small shifts in potency (0.38- to twofold of the original LD50 values).

Animals↗

Respiratory gas exchange in the flea Xenopsylla conformis (Siphonaptera: Pulicidae).

Respiratory gas exchange was measured in various developmental stages of the flea Xenopsylla conformis mycerini (Rothschild, 1904) originating from the central Negev in Israel. Gas exchange in fleas was measured using a flow-through respirometry system that monitored CO2 emission. Lowest metabolic rates were found in the cocooned stage, which included prepupae, early stage pupae, late stage pupae, and preemerged adults. Newly emerged adults and feeding larvae had metabolic rates 2.5-3.0 times greater than those for the cocooned stages. Highest rates of gas exchange were found in feeding fleas. The low metabolic rates of the cocooned stages is thought to contribute to their ability to survive for longer periods than free-living larvae and adults.

Animals↗

Comparative evaluation of juvenoids for control of cat fleas (Siphonaptera: Pulicidae) in topsoil.

The efficacy of a photostable formulation of methoprene and two photostable juvenoids, fenoxycarb and pyriproxyfen, and their residual activity in inhibiting the emergence of adult cat fleas, Ctenocephalides felis (Bouché), was studied in topsoil. Nursery pots composed of clay, peat, and plastic, and wooden flats were used to hold soil samples. Treated soil samples were exposed to sunlight during the 63-d study period. Methoprene was as effective as fenoxycarb and pyriproxyfen against cat fleas for up to 42 d in clay, peat, and plastic pots at a concentration of 64.56 mg (AI)/m2 (6 mg [AI]/ft2), but its activity declined significantly thereafter. In contrast, fenoxycarb and pyriproxyfen showed strong residual activity for the entire 63 d. The activity of methoprene declined even more rapidly over time in wooden flats, while at the same concentrations the other two juvenoids showed significant residual activity for 63 d. Clay, peat, and plastic pots were therefore considered to be equally effective for evaluating the outdoor efficacy of juvenoids in comparison to the wooden flats. However, results obtained with wooden flats may be more realistic when testing residual activity of volatile chemicals such as methoprene. Fenoxycarb and pyriproxyfen showed strong efficacy and residual activity at concentrations of 8.07, 16.14, and 32.28 mg (AI)/m2, whereas methoprene did not cause a significant reduction of adult emergence at levels below 64.56 mg (AI)/m2. LC50 values for methoprene, fenoxycarb, and pyriproxyfen needed for preventing flea emergence when applied to topsoil were estimated to be 0.643, 0.031, and 0.028 ppm, respectively.

Animals↗

Imidacloprid treatment of marsupials for fleas (Pygiopsylla hoplia).

Two eastern barred bandicoots (Perameles gunnii), two eastern quells (Dasyunrus viverrinus), four fat-tailed dunnarts (Sminthopsis crassicaudata), a Leadbeater's possum (Gymnobelideus leadbeateri), a yellow-bellied glider (Petaurus australis), and a ring-tailed possum (Pseudocheirus peregrinus) were treated with imidacloprid (10.7 +/- 0.6 mg/kg topically, minimum dose 0.5 mg) after observation of fleas (Pygiopsylla hoplia) on animals and in their nest boxes. No live fleas or adverse reactions to imidacloprid were observed for 27 days following treatment. The ease of application, absence of toxicity, and long residual effect makes imidacloprid a useful drug for treating flea infestations in these marsupials.

Administration, Topical↗

A flea-associated Rickettsia pathogenic for humans.

A rickettsia named the ELB agent, or "Rickettsia felis," was identified by molecular biology techniques in American fleas in 1990 and later in four patients from Texas and Mexico. We attempted to isolate this rickettsia from infected fleas at various temperatures and conditions. A representative isolate of the ELB agent, the Marseille strain, was characterized and used to develop a microimmunofluorescence test that detected reactive antibodies in human sera. The ELB agent was isolated from 19 of 20 groups of polymerase chain reaction-proven infected fleas. The microimmunofluorescence results provided serologic evidence of infection by the ELB agent in four patients with fever and rash in France (2) and Brazil (2), supporting the pathogenic role of this rickettsia. Our successful isolation of this rickettsia makes it available for use in serologic tests to determine its clinical spectrum, prevalence, and distribution.

Animals↗

Fleas (Siphonaptera) Infesting Small Mammals From The Western Oriental Region.

Nine species of fleas representing 4 families were collected in 1972 form 14 species of small mammals: 2 hedgehogs, 1 shrew, 2 bats, 8 rodents and 1 mongoose. Most specimens were from 7 localities in Karachi District. Xenopsylla astis accounted for 78% of the 404 fleas collected. Collection data, especially flea-mammal associations and locality records, are presented and discussed.

Journal Article↗

Efficacy of orally administered powdered aloe juice (Aloe ferox) against ticks on cattle and ticks and fleas on dogs.

The efficacy of orally administered powdered aloe juice (Aloe ferox) was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus) decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg/kg body weight and those in another 25 mg/kg body weight of powdered aloe juice incorporated in game maintenance pellets, while the animals in the 3rd group received only pellets. Detached female ticks were collected daily and counted and the weights and the fertility of groups of 50 engorged female ticks collected from the animals were ascertained. The powdered aloe juice in the game maintenance pellets had no effect on the tick burdens of the calves or on the fertility of the ticks. Six dogs, in each of 2 groups, were treated daily for 15 consecutive days, commencing on Day -5 before the 1st tick infestation, with either 0.39 g or 0.74 g of powdered aloe juice, administered orally in gelatin capsules, while a 3rd group of 6 dogs served as untreated controls. All the dogs were challenged with Haemaphysalis leachi on Days 0 and + 7, and with Ctenocephalides felis on Days + 1 and + 8, and efficacy assessments were made 1 day after flea and 2 days after tick challenge, respectively. Treatment was not effective against ticks or fleas on the dogs.

Administration, Oral↗