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At least 397 records · Page 22Linked to original sources
PubMed2004

Spatio-temporal dynamics of the egg-laying-inducing peptides during an egg-laying cycle: a semiquantitative matrix-assisted laser desorption/ionization mass spectrometry approach.

The activity-dependent release of peptides from the neuro-endocrine caudodorsal cell (CDC) system of the freshwater snail Lymnaea stagnalis regulates egg laying and related behaviors. In this study, we optimized a mass spectrometry-based approach to study the spatio-temporal dynamics of peptides that are largely derived from the CDC hormone precursor during an egg-laying cycle and a CDC discharge in vitro. Semi-quantitative peptide mass profiling using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) indicated a massive depletion of peptides from the neurohemal area in the cerebral commissure (COM) during egg laying and the existence of a reserve pool of peptides in the CDC somata that were transported to the COM to restore peptide levels. The depletion of CDC peptides from the COM was correlated to their release during an induced electrical discharge in vitro. Moreover, MALDI-MS of the releasate revealed extensive truncation of the carboxyl terminal peptide. Finally, two novel peptides of 1788 and 5895 Da, not encoded by the CDC hormone precursor, also exhibited temporal quantitative changes similar to those of CDC peptides. Sequencing of the peptide of 1788 Da by tandem mass spectrometry yielded the novel sequence HF(FH)FYGPYDVFQRDVamide. Together, this implicates a more complex set of CDC peptides for the regulation of egg laying than previously anticipated.

Amino Acid Sequence↗
PubMed1997

Prevalence of Toxocara spp. eggs in sandpits of parks in Osaka city, Japan, with notes on the prevention of egg contamination by fence construction.

Toxocara spp. eggs were detected from 30 (75%) out of 40 sandpits of parks, in Osaka city. This prevalence was higher compared to those reported other areas of Japan. Since we examined a large quantity of sample, this could have resulted to higher prevalence. The number of eggs recovered decreased following fence construction around sandpits, but it did not sufficiently prevent the contamination of eggs. Improvement of fence design and education of sandpit users are necessary when contemplating fence construction around sandpits as a measure against contamination with Toxocara spp. eggs.

Animals↗
PubMed1983

Induction of collagen synthesis in cultured human fibroblasts by live Schistosoma mansoni eggs and soluble egg antigens (SEA).

There is a dearth of knowledge on the tissue fibrosis that contributes to the pathology of schistosomiasis mansoni. The present study was designed to test the direct effect of live schistosome eggs and soluble egg antigens (SEA) on cultured normal human fibroblasts. Coincubation for 3 days of fibroblast monolayers with 100-500 live eggs/ml medium or equivalent amounts of SEA caused enhanced incorporation of labelled proline. The newly synthesized polypeptides were sensitive to purified collagenase enzyme activity. Collagen synthesis was also verified by measuring increased hydroxyproline content in fibroblasts. Whereas low numbers of eggs stimulated fibroblast activity, 500 eggs/ml medium caused a disarray in the arrangement of cells, with cytoplasmic granulation, cell detachment and death. These results indicate that fibroblast stimulation and collagen synthesis may also be triggered by the direct action of egg secretions on tissue fibroblasts.

Animals↗
PubMed1975

[Variability of the eggs of the local silkworm (Bombyx mori 1.) according to the capacity for thermal parthenogenesis and heat resistance. Report 3. Causes and characteristics of the variability of egg batches according to heat resistance].

The variability of eggs from different batches was studied by the resistance against the sublethal temperature (48 degrees) at the early stages of embryogenesis. The variability of heat resistance at three developmental stages markedly differing by the organization of the embryo was shown to be, to a great extent, conditioned hereditarily and depend as well on the environmental conditions. On the basis of positive correlation between the heat resistance of eggs from the same parents but at different developmental stages, a conclusion is drawn that the variability of heat resistance of the egg cellular proteins is one of the main causes of the variability of egg batches by heat resistance.

Animals↗
PubMed1985

Ultrastructure of eggs of Ascaris lumbricoides Linnaeus, 1758. I. Egg-shells.

Under the light microscope the chitin-protein layer of egg-shells in ascarids appears to be a regular, hyaline and nonstructural layer of 1.5 to 2.00 microns in thickness. The outer uterine layer is usually removed during the preparation. The lipid (ascaroside) layer covers the inner surface of the chitinous layer and seems to be irregularly undulated and regularly thick over the whole surface, with the thickness up to 1 micron. In electron micrographs the fibrous structure of the lipid layer is not evident as a rule. This is probably due to washing the lipids away from this layer during the dehydration of deeper layers of egg-shells that are imperfectly fixed with glutaraldehyde. A very low permeability of the egg-shells is typical of geohelminth eggs. The layer lipid shows a distinct lamellate structure only after a prolonged fixation with osmium at higher temperature. This is supported by the studies using the method of freeze-fracturing.

Animals↗
PubMed2005

Simultaneous determination of nine fluoroquinolones in egg white and egg yolk by liquid chromatography with fluorescence detection.

A liquid chromatographic (LC) method with fluorescence detection was developed for determination of nine fluoroquinolones (FQs) in egg white and yolk. Egg white samples were deproteinized with acidified ethanol (egg yolk samples with acetonitrile and acidified ethanol), followed by defatting with hexane once (white) or twice (yolk), and extracting FQs into acetonitrile. After acetonitrile was evaporated, the residue was dissolved in mobile phase, and FQs were detected in LC with a fluorescence detector. Recoveries for nine FQs from white and yolk were 74.7-85.6%, 79.1-91.2%, respectively, with excellent relative standard deviations. The limits of quantification were 5-20 ngg(-1).

Animals↗
PubMed1987

Glycerolation and thawing effects on bull spermatozoa frozen in detergent-treated egg yolk and whole egg extenders.

Bull sperm in Experiment 1 were added to a standard egg yolk-Tris extender containing 0, .25, .5, 1.0, 1.5, and 2.0% (vol/vol) of the detergent mixture, sodium and triethanolamine lauryl sulfate. Glycerol was added in one step to the initial extender or in three steps after cooling semen to 4 degrees C. The extended semen was packaged in .5-ml French straws and frozen over static nitrogen vapor. Thawing was at 4, 30, and 60 degrees C. There was little difference due to the method of adding glycerol provided detergent was present. Percentages of motile sperm after freezing in the six concentrations of detergent, in ascending order, were 31, 45, 52, 51, 48, and 36. Percentages of motile sperm following thawing at 4, 30, and 60 degrees C were 36.5, 46.0, and 48.6. Acrosome retention also was better preserved with the higher thaw temperatures. A second experiment, similar in design to the first one, was conducted with whole egg-Tris extender. The best results were in whole egg-Tris containing .125% detergent where post-thaw motile sperm of 70.4% greatly exceeded 30.7% without sodium and triethanolamine lauryl sulfate. Fertility of sperm frozen in yolk-Tris-detergent was equivalent to whole milk used as a control.

Animals↗
PubMed1983

The relationship between body weight loss during an induced molt and postmolt egg production, egg weight, and shell quality in caged layers.

Two trials were conducted to determine the relationship between percent body weight loss (% BWL) during an induced molt and postmolt performance. The molt was induced by fasting and photoperiod manipulation. Four groups with 24, 27, 31, or 35% BWL (Trial 1) or 20, 25, 30, or 35% BWL (Trial 2) were utilized in each trial. Performance was evaluated on the basis of 24 weeks of egg production and determinations of egg weight (EW), specific gravity (SG), and shell weight (SW) conducted during Weeks 8 (Trial 2 only), 18, and 24 following the removal of feed. The 27 and 31% BWL groups of Trial 1 and the 30 and 35% BWL groups of Trial 2 produced significantly more eggs per hen housed than the remaining treatments in their respective trial. Differences in SG and SW at the end of the trial were most pronounced in Trial 1 where the 27 and 21% BWL groups exhibited significantly greater SG and SW at Week 24 than did the 24 or 35% BWL groups. No significant differences occurred for SG at Week 24 of Trial 2, but the 35% BWL group exhibited significantly improved SW when compared to the 25% BWL group with the remaining groups intermediate. The EW was greatest for the 31% BWL and least for the 35% BWL group at 24 weeks in Trial 1 but did not differ in Trial 2. It was concluded that a body weight loss of approximately 27 to 31% produced optimum postmolt performance.

Animals↗
PubMed2002

Comparative use of faecal egg count reduction test, egg hatch assay and beta-tubulin codon 200 genotyping in small strongyles (cyathostominae) before and after benzimidazole treatment.

A survey on benzimidazole (BZ) resistance in small strongyles was performed on three farms in the tenth region in Chile. Samples from a total of 100 horses were tested using the faecal egg count reduction test (FECRT), the egg hatch assay (EHA) and an allele-specific PCR for the detection of beta-tubulin isotype 1 genes coding for phenylalanine (phe) or tyrosine (tyr) at codon 200. In the past, BZ-type drugs have been used within anthelmintic campaigns on all the three farms. This has predictably led to a high degree of BZ resistance at the Valdivia and Riñihue farms and to a lesser degree at the Frutillar farm, as demonstrated by all the three tests. The FECRT indicated resistance in every farm by faecal egg count reductions (FECR) of 27% (S.D. +/- 33), 26.5% (S.D. +/- 26.9) and 83.9% (S.D. +/- 22.8) for the Valdivia, Riñihue and Frutillar farms, respectively. With the EHA, the following mean LD(50) values were found before and after treatment with fenbendazole (FBZ): 0.093, 0.141 and 0.066 microg TBZ/ml and 0.149, 0.158 and 0.091 microg TBZ/ml, respectively, for the Valdivia, Riñihue and Frutillar samples. The corresponding LD(96) values were 0.222, 0.263 and 0.188 microg TBZ/ml before treatment and 0.316, 0.322 and 0.221 microg TBZ/ml after treatment, indicating BZ resistance in all the cases. Genotyping was performed on more than 1700 single larvae, at least 10 per faecal sample, for 98 pre- and 66 post-treatment samples. Despite a general trend toward higher percentages of phe/tyr and tyr/tyr individuals following treatment, no statistically significant difference was found between these two and the phe/phe genotype percentages. However, a significantly negative correlation was detected between the LD(50) values and the phe/phe percentages and there was a positive correlation between the FECRT results and the phe/phe percentages. Thus, there seems to be a difference in the significance of the codon 200 polymorphism in the mechanisms of BZ resistance in small strongyles of the horse and sheep trichostrongyles.

Animals↗
PubMed2003

Effects of F-strain Mycoplasma gallisepticum inoculation at twelve weeks of age on egg yolk composition in commercial egg laying hens.

In two trials, the effects of F-strain Mycoplasma gallisepticum (FMG) on the contents of egg yolks from commercial Single Comb White Leghorn laying hens were investigated over a production cycle. Ten hens were assigned to each of 8 (trial 1) or 16 (trial 2) negative pressure fiberglass biological isolation units. Birds in half of the total units served as sham-inoculated controls, and those in the other half were inoculated with FMG at 12 wk of age. Eggs were collected and yolks were harvested at various times during the prepeak, peak, and postpeak periods of both trials for constituent analysis. Yolk constituents analyzed in these trials included moisture, total lipids, cholesterol, triglycerides, phospholipids, and fatty acids. In both trials, total yolk lipid at 22 wk of age was significantly decreased in birds inoculated with FMG. In trial 1, yolk cholesterol at 28 wk was significantly decreased in FMG-inoculated birds. Yolk linoleic acid in trial 1 and yolk stearic and arachidonic acids in trial 2 were significantly increased in FMG-inoculated birds compared to FMG-free birds. In trial 2, yolk myristic, palmitoleic, and oleic acid percentages were significantly decreased in FMG-inoculated birds compared to FMG-free birds. These data suggest that alterations in egg production in commercial layers in response to an FMG infection at 12 wk of age are associated with changes in yolk composition.

Aging↗
PubMed2004

The combined effects of dietary lysine and total sulfur amino acid level on egg production parameters and egg components in Dekalb Delta laying hens.

Dekalb Delta hens were randomly assigned to one of eight dietary treatment groups. Two intakes of lysine (860 and 959 mg/hen per day) and 4 intakes of TSAA (635, 689, 811, 877 mg/hen per day) were combined in a 2 x 4 factorial treatment arrangement and fed from 20 to 43 wks of age. A phase feeding regimen was implemented at 43 wk with lysine intake lowered to 715 or 816 mg/hen per day and TSAA to 578, 607, 699, or 779 mg/hen per day. Cage was the experimental unit (5 hens/cage), and dietary treatments were replicated 8 times. Egg production (EP) and feed consumption were not affected by dietary treatments. Feed efficiency improved linearly by increasing TSAA intake during phase I only. Hen weight gain was improved (P < or = 0.03) by increased dietary lysine (94.2 vs. 135.2 g weight gain/hen). During phase I, hen weight gain was affected quadratically (P < or = 0.02) by TSAA. Increasing TSAA intake up to 689 mg/hen per day increased hen weight gain, but gain decreased at the highest intake. Egg weights (EW) increased (P < or = 0.02) from 59.02 to 60.21 g with increased lysine intake. Increasing lysine intake increased wet and dry albumen percentage, whereas dry yolk percentage decreased with increasing lysine. Total sulfur amino acid intake affected wet yolk, dry yolk, and solids in a quadratic trend, with hens fed 811 and 699 mg/d producing eggs with the greatest yolk solids. Wet and dry shell percentages were not affected by lysine or TSAA, and specific gravity decreased linearly during phase II and overall, with increased dietary TSAA. In conclusion, the dietary lysine at 959 and 816 mg/hen per day for phases I and II, respectively, optimized EW and feed efficiency. Because EP was not affected by dietary lysine, the dietary level for optimizing EP is closer to 860 and 715 mg/hen per day for phases I and II, respectively. Dietary TSAA level for maximum EP and feed efficiency was near 811 and 699 mg/hen per day but for EW may be closer to 877 and 779 mg/hen per day for phases I and II, respectively.

Amino Acids, Sulfur↗
PubMed2005

Comparative clinical and ultrasound study of egg-negative and egg-positive individuals from Schistosoma mansoni low morbidity endemic areas, and hospitalized patients with hepatosplenic disease.

Two hundred and twenty three subjects from a Schistosoma mansoni low morbidity endemic area and nine hospitalized hepatosplenic patients were submitted to stool test and clinical examination and abdomen ultrasound assessments. According to stool examination and ultrasound results, they were grouped as follows: G1 -- 63 Schistosoma mansoni egg-negative individuals; G2 -- 141 egg-positive patients and without evidence of periportal fibrosis; G3 -- 19 egg-positive patients with periportal echogenicity (3-6 mm); and G4 -- 9 hepatosplenic patients with periportal echogenicity (> 6 mm). Hepatomegaly detected by physical examination of the abdomen evaluated in the midclavicular line was verified in G1, G2 and G3, respectively, in 11.1, 12.1 and 26.3%. In G1, G2 and G3, periportal thickening occurred only in schistosomal patients (8.5%). Mild pathological alterations in patients that cannot yet be detected by clinical examination were detectable in the liver by ultrasound and can be due to fibrosis. The degree of mild periportal fibrosis was diminished in 57.9% of patients 12 months after treatment of schistosomiasis with oxamniquine. At ultrasonography, the mean liver left lobe measurement of G3 was larger than that of G1, and that of G4 larger than that of G1 and G2. The mean size of the spleen of G4 was significantly larger than that of the other three groups, and that of G3 larger than that of G1 and G2.

Adolescent↗
PubMedDate not supplied

Egg white-specific IgE and IgG subclass antibodies and their associations with clinical egg hypersensitivity.

The data obtained so far indicate that 1) presence of high titer of IgE antibodies was related with symptoms of egg hypersensitivity, 2) it seems unlikely that increased IgG4 antibody levels against egg white is a cause of egg hypersensitivity, and one should pay much attention to IgG1 antibodies, instead, 3) it is possible that increased IgG4 may reduce the effect of complement-fixing antibodies like IgG1 and/or interfere the action of IgE antibodies.

Adult↗
PubMed2004

Egg quality assurance programs and egg-associated Salmonella enteritidis infections, United States.

A Salmonella enterica serovar Enteritidis epidemic in the United States began in 1978, spread to much of the country in the following decade, and began declining in 1996. We examined correlations between annual changes in S. Enteritidis incidence in humans and introductions of egg quality assurance programs (EQAPs) in some states to reduce S. Enteritidis contamination of eggs. Before EQAPs, 62% of the changes in S. Enteritidis incidence were higher than the baseline for each state. After EQAPs, 73%-84% of the changes were below the baseline. Regression analysis showed that a 1% increase in the number of eggs produced under an EQAP was associated with a 0.14% decrease in S. Enteritidis incidence (p < 0.05). These data indicate that EQAPs probably played a major role in reducing S. Enteritidis illness in these states.

Animals↗
PubMed1992

[Comparative study on the effectiveness of modified Kato's cellophane thick smear and Stoll's dilution egg counting technique for quantitative fecal examination of helminth eggs].

A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E.C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T. in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were 47.86 x 10(0.87) logM.C.T.S. in A. lumbricoides, 41.69 x 10(0.82) logM.C.T.S. in T. trichiura and 63.10 x 10(0.85) logM.C.T.S. in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.

Cellophane↗
PubMed1979

Effect of selection for high egg production in chickens on shedding of lymphoid leukosis virus and gs antigen into eggs.

Lymphoid leukosis virus (LLV) and group specific (gs) viral antigen were detected less frequently in albumen of eggs from two strains of Single Comb White Leghorns that had been selected for high egg production than in corresponding random-bred control strains, which represented the original base population. In a third selected strain, for which no comparable control strain was available, the frequency with which LLV and gs antigen were detected was similar to the other two selected strains. The greatest contrast was between selected Strain 1 and control Strain 5 in which the percentage of eggs with LLV in albumen was 1.4 and 21.4, respectively, and the percentage with gs antigen was 1.2 and 19.9. These differences between control and selected strains of chickens were not related to genetic cellular resistance to virus infection, because inoculation of chorioallantoic membranes with Rous sarcoma virus of subgroups A and B revealed that the proportion of birds resistant to subgroups A and B viruses was not greater in the selected strains than in the control strains.

Animals↗
PubMed1984

Physiological profile of caged layers during one production year, molt, and postmolt: egg production, egg shell quality, liver, femur, and blood parameters.

A longitudinal study of a flock of Single Comb White Leghorn pullets was initiated at 19 weeks of age (preproduction) and continued through a production year, a forced molt, and for 4 months of postmolt production. A representative sample of hens was obtained at 12-week intervals during the first year and at subsequent selected times. Liver lipid, femur weight, femur volume, femur density, egg weight, shell weight, percent shell, milligrams shell/square centimeter of shell surface area, serum calcium, serum phosphorus, and serum alkaline phosphatase were determined. Percent hen-day production peaked at 90% and then declined by .6 to .7% each week during the first production year. After molting, percent hen-day egg production peaked at 80% and declined .9% per week over the subsequent 20 weeks. Egg weight increased continually during the first production year. Shell weight was greatest immediately postmolt; thereafter it declined. Shell thickness was greatest at 31 weeks of age and declined throughout the first year. After molting, the shell thickness of 83-week-old hens was similar to values of hens about 37 weeks of age. Serum calcium and phosphorus of laying hens were influenced by age, feed intake and environmental temperature. The lowest values occurred during hot weather. Liver lipid was lowest in nonlaying hens (17 to 20%) and was approximately 42% of dry weight in laying hens. Femur density was greater in laying than nonlaying hens.

Alkaline Phosphatase↗