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PubMed1974

Evaluation of micro complement fixation tests for antibodies against group A streptococcal M and M-associated antigens in rabbit and human sera.

Variables involved in micro complement fixation (CF) tests for type-specific (TSM) and non-type-specific (NTSM) antibodies against preparations of streptococcal M protein were studied. Sera of rabbits immunized with purified M protein which contained high titers of anti-TSM and low titers of anti-NTSM antibodies reacted type specifically in the CF tests when relatively low concentrations of M protein were employed. In contrast to these artificially induced TSM antibody responses, the sera of rheumatic fever patients demonstrated elevated CF antibody titers (1:80 to 1:320) in the presence of both high and low concentrations of M antigen. The CF test cannot distinguish TSM and NTSM antibodies when the latter predominate. The CF test can be employed with confidence for the detection of NTSM antibodies, provided that the test sera are known to lack type-specific antibodies against the M protein used in the test.

Animals↗
PubMed1975

New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen.

A complement-fixation (C.F.) test for the human reovirus-like agent of infantile gastroenteritis has been developed using the serologically related Nebraska calf diarrhoea virus (N.C.D.V.) as antigen. Most infants and children who shed the agent in stools and/or who demonstrated serological (C.F.) evidence of infection with a reovirus-like-particle-positive human stool-filtrate C.F. antigen also demonstrated serological evidence of infection when a concentrated N.C.D.V. preparation was employed AS C.F. antigen. The N.C.D.V., which was previously shown to be related to the human reovirus-like agent, was found to be related antigenically to the epizootic diarrhoea of infant mice (E.D.I.M.) virus also. Studies on the prevalence of C.F. antibody in sera from infants and young children revealed a pattern of rapid acquisition of antibody to both the human reovirus-like agent and the N.C.D.V. as over 80 percent of these individuals possessed antibody to each agent by 36 months of age. A strong positive association was found in the results obtained with the two antigens. The ready availability of cell-culture grown N.C.D.V., and its ability to serve as a "substitute" C.F. antigen for the human reovirus-like agent, should enable the serodiagnosis of many cases of disease due to the human agent and facilitate seroepidemiological studies of such infections. In addition, the observation that a large proportion of individuals infected with the human reovirus-like agent develop serological evidence of infection not only to the human agent but to the calf agent as well may have important implications in the immunoprophylaxis of disease caused by the human reovirus-like agent.

Animals↗
PubMedDate not supplied

[Determination of antibody kinetics in Mediterranean spotted fever using the Weil-Felix agglutination test, complement fixation and indirect immunofluorescence].

The kinetics of antibodies in human cases of Mediterranean spotted fever was studied by applying Weil-Felix (WF) agglutination with Proteus OX antigens, complement fixation (CF) and indirect immunofluorescence (IF) tests with Rickettsia conorri antigen to 46 sera from 21 patients. The kinetics of specific (CF and IF) and nonspecific (WF) antibodies was similar. The percentage of the positive sera and geometric mean titers of antibodies reached the maximum after 14 days from the onset of illness.

Agglutination Tests↗