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Potential for escape of live boll weevils (Coleoptera: Curculionidae) into cottonseed, motes, and cleaned lint at the cotton gin.

Reintroductions of the boll weevil, Anthonomus grandis grandis Boheman, into areas of the United States where it has been eradicated or suppressed are very expensive to mitigate. There is concern that a cotton gin in an eradication zone may serve as a site of boll weevil reintroductions when processing cotton harvested in a neighboring infested zone. Similarly, there is a question whether weevil-free areas can safely import gin products, such as cottonseed and baled lint, from infested areas without risking an introduction. Many countries require fumigation of imported U.S. cotton bales to protect against boll weevil introductions, costing the U.S. cotton industry millions of dollars annually. In previously reported experiments, we quantified the potential for boll weevils to survive passage through precleaning machinery in the gin. In this study, we quantified survival potential of boll weevils passing through the gin stand and segregating into the cottonseed, mote, or lint fractions. We also examined boll weevil survival when passed with ginned lint through a lint cleaner. We present a flow chart of experimentally determined survival potentials of boll weevils passing through the various subprocesses of the gin, from which one can calculate the risk of a live boll weevil reaching any point in the process. Our data show that there is virtually no chance of a boll weevil being segregated alive into the cottonseed or of one surviving in the lint to approach the bale press. Therefore, quarantine or fumigation of cottonseed and cotton bales to guard against boll weevil introductions is unnecessary.

Agriculture↗

Season-long variation in expression of Cry1Ac gene and efficacy of Bacillus thuringiensis toxin in transgenic cotton against Helicoverpa armigera (Lepidoptera: Noctuidae).

The insertion of genes encoding insecticidal Cry1A delta-endotoxins from the bacterium Bacillus thuringiensis Berliner variety kurstaki (Bt) into varieties of cotton (Gossypium spp.) was undertaken to assist in the control of a range of lepidopteran pests. In Australia, where Helicoverpa armigera (Hübner) and Helicoverpa punctigera (Wallengren) are major pests, the level of control is useful, but not complete, because efficacy declines as the crop matures. Fluctuations in the efficacy of Bt cotton, to the extent that some insects survive, provide opportunities for H. armigera to develop resistance to the Bt toxin. Therefore, variations in the efficacy of Bt cotton need to be understood if we are to plan rational resistance management strategies to retard the rate of the development of resistance. We measured the changes in efficacy associated with plant development over the growing season, in the field and glasshouse. In addition, the levels of Cry1Ac protein toxin and cry1Ac RNA were determined. In this first demonstration of the relationship between these three factors, we found that the developmental decline in bioefficacy in field-grown plants was associated with reduced cry1Ac transcript levels and Bt toxin levels in postsquaring cotton. In addition, changes in plant chemistry associated with the maturation of the cotton plant were observed to contribute to changes in the efficacy of Bt toxin. Results from the field and glasshouse suggested that variations in efficacy within the growing season and between seasons also may be influenced by environmental factors.

Animals↗

DNA screening reveals pink bollworm resistance to Bt cotton remains rare after a decade of exposure.

Transgenic crops producing toxins from the bacterium Bacillus thuringiensis (Bt) kill insect pests and can reduce reliance on insecticide sprays. Although Bt cotton (Gossypium hirsutum L.) and Bt corn (Zea mays L.) covered 26 million ha worldwide in 2005, their success could be cut short by evolution of pest resistance. Monitoring the early phases of pest resistance to Bt crops is crucial, but it has been extremely difficult because bioassays usually cannot detect heterozygotes harboring one allele for resistance. We report here monitoring of resistance to Bt cotton with DNA-based screening, which detects single resistance alleles in heterozygotes. We used polymerase chain reaction primers that specifically amplify three mutant alleles of a cadherin gene linked with resistance to Bt cotton in pink bollworm, Pectinophora gossypiella (Saunders), a major pest. We screened DNA of 5,571 insects derived from 59 cotton fields in Arizona, California, and Texas during 2001-2005. No resistance alleles were detected despite a decade of exposure to Bt cotton. In conjunction with data from bioassays and field efficacy tests, the results reported here contradict predictions of rapid pest resistance to Bt crops.

Alleles↗

Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.).

Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.

2,4-Dichlorophenoxyacetic Acid↗

Probabilistic risk assessment of cotton pyrethroids: IV. Landscape-level exposure characterization.

Aquatic exposure arising from the use of pyrethroid insecticides on cotton in the United States was estimated as part of an extensive pyrethroid aquatic risk assessment. The exposure analysis was based on the standard U.S. Environmental Protection Agency tier II regulatory scenario for cotton, which assumes a 1-ha pond surrounded by 10 ha of treated crop, with high levels of runoff, erosion, and drift entering the pond. This regulatory scenario was modified to include simplified landscape-level information on the proximity of cotton to ponds derived from a remote sensing study. This scenario also accounts for the no-spray buffers between pyrethroid applications and surface waters mandated on all cotton pyrethroid labels (which differentiate applications made by air and by ground-based equipment), and for the percentage of cotton area that is treated with pyrethroids. Incorporation of these landscape-level factors into the analysis reduced the predicted aquatic exposure concentrations approximately 50- to 100-fold. Because many other conservative assumptions in the original tier II exposure analysis were not revised, the modified exposure predictions are still overestimates of true field exposure concentrations.

Animals↗

Broiler litter as a micronutrient source for cotton: concentrations in plant parts.

Analytically, poultry litter contains nearly all essential micronutrients but the extent of phytoavailability of these nutrients and whether cotton (Gossypium hirsutum L.) and other crop plants can receive adequate amounts of these nutrients from litter is not fully known. The objective of this research was to determine whether cotton receives sufficient amounts of Fe, Cu, Mn, and Zn from litter and estimate the efficiency of cotton in extracting these metal nutrients from litter in the absence of any other source of the micronutrients. The greenhouse research used plastic pots filled with approximately 11 kg of a 2:1 (v/v) sand to vermiculite growing mix. Cotton (cv. Stoneville 474) was grown in the pots fertilized with broiler litter at rates of 30, 60, 90, or 120 g pot(-1) in a factorial combination with four supplemental nutrient solution (NS) treatments. The nutrient solutions consisted of full Hoagland's nutrient solution (NS-full); a solution of the macronutrients N, P, K, Ca, and Mg (NS-macro); a solution of the micronutrients Fe, Zn, Mn, Cu, B, and Mo (NS-micro); and water (NS-none). Based on tissue nutrient analysis, a one-time broiler litter application supplied adequate amounts of Fe, Cu, and Mn to bring the concentration of these nutrients in upper leaves within published sufficiency ranges. Zinc, with <17 mg kg(-1) concentration in the upper leaves, was the only micronutrient below the established sufficiency range regardless of the rate of applied litter. Cotton extracted Fe and Mn more efficiently than Cu or Zn, removing as much as 8.8% of Fe and 7.2% of Mn supplied by 30 g litter pot(-1). In contrast, the extraction efficiency was 1.7% for Cu and 1.9% for Zn. Roots accumulated 58% of the total absorbed Fe and 64% of Cu, and leaves accumulated 32% of the Fe and only 13% of the Cu supplied by litter. In contrast, only 16% of the total absorbed Mn and 23% of Zn accumulated in roots while leaves accumulated 64% of the total Mn and 37% of Zn. These results demonstrate that broiler litter is a valuable source of the metal nutrients supplying Fe, Cu, and Mn in full and Zn in part, but a very large fraction of the litter-supplied metal nutrients remained in the growing mix.

Agriculture↗

Lung function, airway reactivity, and atopy in newly hired female cotton textile workers.

To assess changes in lung function and airway reactivity resulting from exposure to cotton dust, and the role of atopic status in these changes, the authors observed a group of 225 newly hired Chinese textile workers for 1 yr. All workers were female, lifelong nonsmokers, and none of them had been exposed previously to cotton or other occupational dust. Atopic status was determined at baseline. Spirometry, response to methacholine challenge, and total serum immunoglobulin E level were examined at baseline and again after subjects began work in the cotton mills. Obvious cross-shift drops in forced expiratory volume in 1 sec (FEV1.0), and declines in forced vital capacity and FEV1.0 over 1 yr, were observed. Atopic workers had a significantly greater acute drop in FEV1.0 than did nonatopic workers. Both atopic and nonatopic workers had slightly increased airway reactivity at 1 yr, compared with baseline values. The results suggest that exposure to cotton dust is responsible for acute and longitudinal declines in lung function, as well as for slightly increased airway reactivity. Atopy may interact with cotton dust to accentuate the acute lung function response.

Adolescent↗

Respiratory disease in cotton textile workers in the People's Republic of China. I. Respiratory symptoms.

The prevalence of byssinosis and nonspecific respiratory symptoms was studied in 887 textile workers with at least two years of employment in two cotton mills and one silk mill in Shanghai, the People's Republic of China. A standardized respiratory questionnaire was used, and environmental sampling was performed with vertical elutriators and colorimeter grading. Eight percent of the cotton textile workers complained of byssinosis. The reports of byssinosis were mostly mild (grade 1/2), more prevalent among women, and unrelated to duration of employment or elutriator dust levels. Nonspecific respiratory symptoms were significantly more prevalent among cotton textile workers than silk workers. After adjustment for age, gender, and smoking in logistic regression models, the odds ratios for the effect of working in cotton textile mills on chronic bronchitis, chronic cough, and frequent chest illness were 3.3, 2.9 and 4.7, respectively. Although none of the symptoms were related to current dust levels, the range of exposures was narrow, and information was only available on current levels of cotton dust. This study represents the first respiratory survey of the textile industry in China using diagnostic criteria similar to that used in the United States and England; it defines a cohort for prospective investigation.

Adult↗

Neuroendocrine (ECL cell) differentiation of spontaneous gastric carcinomas of cotton rats (Sigmodon hispidus).

BACKGROUND AND PURPOSE: Female inbred cotton rats develop adenocarcinomas in the oxyntic mucosa. Since a female preponderance is typical for enterochromaffin-like (ECL) cell tumors, we examined such tumors for ECL cells. Gastrin plays a decisive role in ECL cell tumorigenesis, so blood gastrin concentration and gastric mucosal pH were measured. METHODS: The stomachs from six female cotton rats (6 to 8 months old) were studied histologically, and at euthanasia, gastric mucosal pH was determined. Euthanasia was performed on 15 other female cotton rats of similar age for determination of blood gastrin values by radioimmunoassay (RIA) and gastric mucosal pH. Rats were classified macroscopically to have normal or thick oxyntic mucosa, with or without tumor. RESULTS: Among the six cotton rats studied histologically, two 6-month-old rats had normal and two others had thick gastric mucosa, whereas two 8-month-old rats had thick mucosa with tumors. The ECL cells were markedly hyperplastic in all rats with thick mucosa, and ECL cells were found in the neoplastic parenchyma. All cotton rats with normal-appearing gastric mucosa had pH <2.5, whereas 14 rats with thick mucosa had pH >3.1 and hypergastrinemia. CONCLUSIONS: Gastrin may play a major role in ECL cell hyperplasia and, perhaps, in adenocarcinoma genesis.

Adenocarcinoma↗

[Determination of tannin in cotton plant].

Three methods were used to determine the tannins content in cotton plant tssues. The tannin content in different organs and terminal leaves of four cotton varieties at different growth stages determined by the Folin's assay was nearly two folds as much as that determined by acid butanol assay, indicating that the Folin's assay only offers the total phenol content, but is not suitable for determining the content of condensed tannin in cotton. There was no significant difference between the results of vanillin assay and acid butanol assay, and hence, it is feasible to use vanillin assay to test the tannin content in cotton. Determinations at boll stage showed that the content of condensed tannin in calyx, boll shell and leaf was rather high, generally up to 5-10%, while that in petal, stigma, ovary and inner boll was lower (about 2%). The condensed tannin content in terminal leaf continually increased from seedling(< 1%) to boll stage (10% or so), indicating that the increased tannin content is closely related to the maturity, decrepitude and lignification of cotton tissues.

Gossypium↗

[Biological control efficiency of ladybirds on arthropod pests in cotton agroecosystems].

Based on the systematical investigation of population dynamics of predacious ladybird in different cotton agroecosystem, the energy flow for predacious ladybirds was analyzed by the way of ecological energetic. The biological control efficiency of ladybirds was compared in different planting-time and inter-crop cotton agroecosystems using available date on the prepapers of ladybird ingestion to pest production. The results showed that the ingestion of predacious ladybirds were 26.45-70 kJ.m-2 yr.-1, accounting for 28.30-47.88% of all predacious natural enemies in different cotton agroecosystems. The biological control efficiency of ladybirds to aphids were 5.07-12.85%, which was decreased in inter-crop cotton agroecosystems and the ecosystems with the delay of planting-time. It is suggested that the ladybirds play the important role of potential biological control in cotton agroecosystems.

Agriculture↗

Antagonistic effects of several bacteria on Verticillium dahliae the causal agent of cotton wilt.

Experiments were carried out with 89 bacterial isolates that were collected from cotton rhizosphere in Gorgan province. The antagonistic effects of bacterial isolates on Verticillium dahliae Klebahn were studied using dual culture test. Five highly effective isolates were selected from these antagonists for subsequent studies. According to the biochemical, physiological and morphological tests, isolates 2020 and 3 were identified as Pseudomonas fluorescens and isolate 204, 202 and 309 as Bacillus spp. These isolates were used to investigate their antagonistic mechanisms in vitro and their effects on cotton growth in vivo. Inhibition of V. dahliae by volatile metabolites and antibiotics was studied as described by Fiddamen (1994) and Kraus (1990). Production of hydrogen cyanide was studied qualitatively, using HCN-indicator paper of Castric and Castric (1983). Isolates 204, 202 and 309 inhibited the mycelial growth of the fungus through production of volatile metabolites. Isolates 2020 and 3 produced antibiotic as well as volatile metabolities that inhibited mycelial growth of V. dahliae. They both produced hydrogen cyanide. After four months of greenhouse study, the application of antagonistic bacteria had different effects on growth of cotton plants. Bacterial treatment in soil had better effects on plant growth than that of bacterial seed treatment. In soil treatments containing infested and non-infested soil with V. dahliae, isolates 2020 and 3 caused an increase in plant height in comparison with those in infested and non-infested controls. In non-infested soil, application of isolates 2020, 3 and 202 increased root length and dry weight of cotton plant, but in soil infested with the fungus, only isolate 202 increased root length. Isolate 2020 increased plant dry weight. In conclusion, isolates 2020 and 3 belonging to P. fluorescens and isolate 202 pertaining to genus Bacillus had the greatest effect on increasing the cotton growth.

Antibiosis↗

[Screening and breeding of highly-effected degrading cotton-phenol strains and study on defoxication technology and conditions].

From mildewed cottonseed cake and stock cultures of mold and yeast. We select more than ten strains of yeasts and molds which can degrade cotton phenol. At last we got four strains which can degrade cotton phenol highly effected after mutagenized by physical and chemical factors and induced by cotton phenol. They belong to Candida tropicalis, Torulopsis candida, Aspergillus flavus and Aspergillus niger. By small and medium size fermenfations, the content of dissociated cotton phenol all reach safe criterion (the lowest dissociated cotton phenol content is 220 mg/L in the defoxicated cottonseed cake), compared with FeSO4 method, defoxicated cottonseed cake looks and tastes well and the content of protein and amino acid are highly enhanced as will.

Aspergillus flavus↗

The association between cotton and prevalence of rural childhood asthma.

In the United States, asthma now ranks as the most prevalent chronic disease among children. Within rural cotton-producing regions, a popular belief is that cotton production increases the prevalence of asthma. This case-control study was conducted to determine if the prevalence of asthma is higher in children living in rural cotton-producing countries than in children where cotton production is negligible. No evidence was found to support the belief that cotton production increases the rural childhood prevalence of asthma (P = .12). More importantly, this study did reveal a significantly higher prevalence of asthma among the fifth graders in rural West Texas than the prevalence predicted by the Centers for Disease Control and Prevention (P < .0001).

Asthma↗

Bacterial adherence to cotton and silk sutures.

BACKGROUND: Silk and cotton sutures are the most commonly used materials for skin closure, the choice being largely based on tradition. We undertook this study to compare the bacterial adherence in vitro to these two materials because it is well known that the physicochemical characteristics of a suture material influence its ability to attract bacteria and consequently promote wound infection. METHODS: We determined the bacterial adherence in vitro to cotton and silk for Staphylococcus aureus and Escherichia coli, common organisms found in postoperative infection at our institute, using three inoculum strengths. The sutures were incubated with the organisms and bacterial counts per suture material calculated after 20, 60, 120 and 180 hours of incubation. The bacterial counts for the sutures were then compared at these intervals. RESULTS: The bacterial adherence for both organisms at all time intervals was significantly greater to silk than to cotton, except at 60 hours for Staphylococcus aureus. The bacterial count for each suture material appeared to be an intrinsic property of the suture and did not vary with the concentration of the bacteria in the initial inoculum. The cost of an equivalent thickness of silk is 50 times that of cotton. CONCLUSION: We suggest that cotton should be the preferred suture for skin closure because bacterial adherence to it is lower and it is much cheaper than silk.

Bacterial Adhesion↗

[Inheritance and segregation of transformants in cotton with two types of insect-resistant genes].

A plant expression vector containing a chemeric Bt29K gene coding for the active Cry1Ac protein and the arrowhead proteinase inhibition gene API-B was introduced into an elite cotton cultivar Jihe 321 by Agrobactertium tumefaciens. Some insect-resistant cotton lines were developed. Segregation and stabilization of insect-resistant genes in six transformation lines were studied. Based on the results of kanamycin resistant test and insect bioassay using Heliethis armigera, PCR detection and Southern-blot, we found that the inheritance and segregation of Bt gene were complicated, some transformants were in accordance with Mendelian patterns of inheritance in the ratio of insect-resistant plants to non-resistant plants in Ti progeny, yet others were non-Mendelian patterns. But the inheritance and segregation of Bt gene in homozygous transformation lines were one or two pairs of major dominant genes through crossing of insect resistant homozygous lines with non-transformation cotton variety. That the insect resistance phenotype was conditioned by one or two pairs of dominant genes was ascertained in this study. There were two copies of Bt genes in two transformation lines DR248 and DR193, which was reported for the first time. The results were confirmed by Southern-blot. Through observation of segregation population of transgenic plants at different generations, we found that the exogenous Bt gene in cotton genome showed unstable in inheritance in early generations, but the gene could be stabilized through resistance screening generation by generation. The unstability of Bt gene may mean that it need time for the gene to compatibilize cotton genome.

Animals↗

[Influence of different NH4+/NO3- ratios on nitrogen metabolism of cotton].

The influence of different NH4+/NO3- ratios on nitrogen metabolism of cotton was studied under controlled hydroponics. The results showed that compared with single nitrate nutrition, solutions with 25/75, 50/50, 75/25 and 100/0 of NH4+/NO3- significantly increased the soluble protein accumulation in leaves and roots of cotton, and the maximum content of soluble protein in leaves and roots appeared respectively in the solution with 50/50 and 75/25 of NH4+/NO3-. The soluble protein content in roots was increased with the increase of NH4+ percentage, but was slightly less in the solution of 100/0 than 75/25, which was probably related to the excess NH4+ limiting boot metabolism. With the increase of NH4+ percentage, the nitrate content in petiole and the nitrate reductase activity in functional blade declined, but ammoniac nitrogen content increased in every organ of cotton. These results showed that foreign nitrogen affected the nitrogen metabolism of cotton in a different way, and the nitrogen absorption by cotton was probably related to different forms of foreign nitrogen.

Dose-Response Relationship, Drug↗

[Cloning of a MADS box protein gene (GhMADS1) from cotton (Gossypium hirsutum L.)].

As a kind of transcription factors, MADS-box protein plays an important role in various cellular processes, especially in the development of floral organs. Based on the contig analysis of the cotton ESTs, the coding region of a cotton MADS-box protein (GhMADS1) was obtained by RT-PCR from floral buds of cotton (G. hirsutum). The cloned fragment of 713 bp (GhMADS1, GenBank accession no. AF538965) contains an open reading frame of 711 bp,coding a polypeptide of 236 amino acids. It was demonstrated that the deduced GhMADS1 protein was highly homologous to the AGL2 group of MADS-box proteins from Vitis vinifera, Nicotiana sylvestris, Petunia hybrida, Arabidopsis thaliana and Antirrhinum majus. Phylogenetic analysis also indicated that GhMADS1 belongs to the AGL2 group of MADS-box proteins. RT-PCR analysis showed that GhMADS1 gene expressed in petals, stamens, ovules and fibers, but not in roots, stems, leaves, bracts and sepals. The strongest expression of GhMADS1 gene was detected in petals. But in floral buds of a cotton homeotic mutant (CHV1), whose floral organs are all converted to bract leaf-like organs, the transcript of GhMADS1 gene was not detected. It was proposed that GhMADS1 gene would be crucial to the development of cotton floral organs.

Amino Acid Sequence↗