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PubMedDate not supplied

[Antibiogram of bacteria of the Clostridium genus].

The disc-agar diffusion method for antibiotic sensitivity testing was adapted to Clostridium. The main feature of the method described here resided in the use of an inoculum which varied according to the physiological properties of the strain tested, so that the same critical time was obtained in all cases. This technique employs commercially available media and reduced equipment. Statistical analysis of the results indicated a strong correlation between disc-agar sensitivity testing and MIC determined by the agar dilution method.

Agar↗
PubMed1984

[The microcomputer in bacteriology. Value in the automated performance of antibiograms in hospitals].

In microbiology, most computerization is carried out by means of important centralized computers. On the contrary, our work is based upon the use of inexpensive microcomputers. We have developed general programs for the acquisition of data, their correction, and retrieval of lists arranged according to desired parameters (hospital department, germ, or sample) and various statistics, especially with respect to germ sensitivity. This system has been used on a routine basis since April 1982 in the Bacteriology Laboratory of Nancy University Hospital. It could easily be adapted to other laboratories and could provide a wide range of essential epidemiological data on the resistance of bacterial strains to antibiotics and their evolution with time.

Computers↗
PubMed1983

[Comparison of the results of antibiograms obtained from blood culture broth and after isolation on agar media with the Autobac 1].

The authors compare the results of antibiotic sensitivity tests performed within 4 hours with the aid of the Autobac 1 machine, using blood culture medium and following isolation on agar plates. There was concordance in about 90 p. cent of cases. The first test is not suitable for sensitivity to trimethoprim and the beta-lactamines, as was already observed in the study using the MS-2 machine. The authors discuss the various sources of error and compare the results obtained from the two machines.

Agar↗
PubMedDate not supplied

[Antibiograms of Leptospira on solid media in Petri dishes].

The sensitivity of fowl leptospirae to antibiotics was tested by diffusiometry on solid media in Petri dishes. The method includes two stages: 1) visible leptospirae cultures are obtained by central seeding and incubation during 3--5 days at 28 or 37 degrees C; 2) antibiotic tablets are disposed at 2 cm from the visible edge of the multiplication zone of the leptospirae. The edge is marked with a pencil at the moment in which the antibiotics are applied. Incubation is continued at the same temperatures. The results are read after five days taking as reference the position of the marginal multiplication line. The sensitivity of leptospirae to antibiotics is expressed by arrested multiplication and resistance by advance of the marginal multiplication line up to or even beyond the antibiotic tablets. Fowl leptospirae are sensitive to penicillin, tetracyclin, chloramphenicol, streptomycin, novobiocin, polymixin, pristinamycin, neomycin, erythromycin, and resistant to mycostatin, septrin, rifampicin, optoquine, sulphatiazol.

Anti-Bacterial Agents↗
PubMedDate not supplied

Antibiogram and production of beta-lactamase by canine isolates of Staphylococcus aureus.

Clinical isolates of canine S. aureus strains (213) have been investigated for ability to produce beta-lactamase and sensitivity to 12 antibiotics. Fifty (23.5%) strains produced beta-lactamase. Using only the paper disc antibiotic diffusion method 17 of these would incorrectly have been classified was sensitive to beta-lactamase antibiotics. all strains were sensitive to methicillin, gentamicin and fusidic acid.

Animals↗
PubMed1980

Mechanized technique for phage typing and determination of antibiograms.

A device for mechanical application of either drops of phage suspensions or antibiotic-containing tablets was developed. The device consists of a Transport Apparatus which transports petri dishes from one side of the apparatus to the other in such a way that the plate and the lid of a petri dish become entirely separated at a particular stage. Either a Phage Applicating System or an Antibiotic Tablet Dispenser can be connected to the apparatus. In this way a maximum of 27 phages of 7 antibiotic tablets per plate can be applied pneumatically.

Anti-Bacterial Agents↗