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Differential replication of satellite DNA in polyploid tissues of Drosophila virilis.

Satellite DNA amounts were examined in adult tissues of Drosophila virilis, a species whose DNA contains three prominent satellites. Satellite amounts in DNA from six of the seven tissues were lower than in DNA from diploid (adult brain) tissue. Satellite amounts in adult ovary DNA, however, were equivalent to or greater than diploid levels. When DNA from pupal ovaries was examined, a 30% increase in satellite amounts over diploid levels was found. An RNA-DNA hybridization experiment showed that the ribosomal RNA genes in pupal ovary DNA were under-replicated relative to diploid DNA levels.

Animals↗

Further studies on polyploid amphibians. V. C-banding in diploid and tetraploid species of Odontophrynus.

C-band patterns of some Odontophrynus species from Brazil are described. O. americanus 2n and 4n are cryptic species similar to O. cultripes 2n, but their C-banding patterns are different. In O. americanus 2n the C-band appears at the secondary constriction of chromosomes 4. In O. americanus 4n and O. cultripes the C-band appears at the secondary constriction of chromosomes 11. O. cultripes chromosomes 2,3 and 6, have also an intercalary band. Furthermore, fine centromeric bands in several weeks old preparations of these species were observed. The possibility of a structural or chemical heterogeneity of those heterochromatic regions is discussed.

Animals↗