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How to "hear" visual disparities: real-time stereoscopic spatial depth analysis using temporal resonance.

In a stereoscopic system, both eyes or cameras have a slightly different view. As a consequence, small variations between the projected images exist ('disparities') which are spatially evaluated in order to retrieve depth information (Sanger 1988; Fleet et al. 1991). A strong similarity exists between the analysis of visual disparities and the determination of the azimuth of a sound source (Wagner and Frost 1993). The direction of the sound is thereby determined from the temporal delay between the left and right ear signals (Konishi and Sullivan 1986). Similarly, here we transpose the spatially defined problem of disparity analysis into the temporal domain and utilize two resonators implemented in the form of causal (electronic) filters to determine the disparity as local temporal phase differences between the left and right filter responses. This approach permits real-time analysis and can be solved analytically for a step function contrast change, which is an important case in all real-world applications. The proposed theoretical framework for spatial depth retrieval directly utilizes a temporal algorithm borrowed from auditory signal analysis. Thus, the suggested similarity between the visual and the auditory system in the brain (Wagner and Frost 1993) finds its analogy here at the algorithmical level. We will compare the results from the temporal resonance algorithm with those obtained from several other techniques like cross-correlation or spatial phase-based disparity estimation showing that the novel algorithm achieves performances similar to the 'classical' approaches using much lower computational resources.

Algorithms↗

Siting MSW landfills with a spatial multiple criteria analysis methodology.

The present work describes a spatial methodology which comprises several methods from different scientific fields such as multiple criteria analysis, geographic information systems, spatial analysis and spatial statistics. The final goal of the methodology is to evaluate the suitability of the study region in order to optimally site a landfill. The initial step is the formation of the multiple criteria problem's hierarchical structure. Then the methodology utilizes spatial analysis processes to create the evaluation criteria, which are mainly based on Greek and EU legislation, but are also based on international practice and practical guidelines. The relative importance weights of the evaluation criteria are estimated using the analytic hierarchy process. With the aid of the simple additive weighting method, the suitability for landfill siting of the study region is finally evaluated. The resulting land suitability is reported on a grading scale of 0-10, which is, respectively, from least to most suitable areas. The last step is a spatial clustering process, which is being performed in order to reveal the most suitable areas, allowing an initial ranking and selection of candidate landfill sites. The application of the presented methodology in the island of Lemnos in the North Aegean Sea (Greece) indicated that 9.3% of the study region is suitable for landfill siting with grading values greater than 9.

Environment↗

Space and spatial frequency: analysis and representation in the macaque striate cortex.

Simple cells in the macaque striate cortex were tested with bars, edges and gratings. Spatial frequency tuning curves could be predicted from the spatial profiles plotted with bars and edges and the bandwidth could be evaluated more accurately by computing the mean from measured and predicted tuning curves. The results suggest that the mean relative spatial frequency bandwidth (delta f/fo) is nearly constant and of a moderate value. But at each optimal spatial frequency, cells with different bandwidths (about a factor of two) were recorded. The shapes of spatial response profiles resemble the corresponding spatial and spatial frequency characteristics of line and edge detectors evaluated psychophysically. Among the remaining cell types, concentric cells tend to be tuned to lower spatial frequencies and have broader bandwidths, whereas periodic cells prefer higher spatial frequencies and have narrower bandwidths. Thus the mean relative bandwidth tends to decrease significantly with spatial frequency (as required by a system of patch-by-patch Fourier analysis) only when cells with poor orientation selectivity and the non-linear silent periodic cells are included along with the simple cells. Simple cells, on their own, seem to form a quasi-linear contrast processing system which is more biased towards spatial accuracy than spatial frequency selectivity.

Animals↗

Polarizing microscopy of Picrosirius stained bone sections as a method for analysis of spatial distribution of collagen fibers by optical diffractometry.

Cross sections of femur diaphysis obtained from control and osteopetrotic rats were stained with hematoxylin-eosin (HE) and Picrosirius (SR). Analogous selected areas of bone sections photographed under a polarizing microscope were analysed by optical diffractometry. Since the collagen fibers are a good marker for the structure of bone tissue, their spatial distribution evaluated by optical diffractometry provides information on the tissue architecture. The Picrosirius staining technique enhances the natural birefringency of collagen fibers. Therefore, in the polarizing microscope, pictures of high contrast are obtained. This procedure, by increasing the amount of information in the image, increases the quantity of the data obtained by optical diffractometry in comparison with the HE staining method. The results obtained prove that SR staining combined with polarizing microscopy might be useful for optical diffractometry in analysis of the spatial distribution of collagen fibers in all connective tissues, where they could serve as markers of tissue architecture.

Animals↗

[The geographical distribution of fertility around Mexico City].

A spatial analysis of fertility around Mexico City reveals an "inverted U" pattern which appears, on the face of it, inconsistent with both conventional economic location analysis and a spatial diffusion process. A closer examination of the problem implies that economic variables, when integrated in a proper model, may account for the observed cross- sectional spatial pattern, whereas pure diffusion can not. Spatial diffusion, however, may be responsible at least in part for the observed fertility changes over time. The geographical patterns of fertility in 2 states neighboring Mexico City (Mexico and Hidalgo) are examined empirically. The interpretation of the nonmonotonic distance effect is that there are significant locational advantages to childbearing at medium distances that are not enjoyed at households location either very close or far away from the city.

Americas↗

Spatial autocorrelation analysis of individual multiallele and multilocus genetic structure.

Population genetic theory predicts that plant populations will exhibit internal spatial autocorrelation when propagule flow is restricted, but as an empirical reality, spatial structure is rarely consistent across loci or sites, and is generally weak. A lack of sensitivity in the statistical procedures may explain the discrepancy. Most work to date, based on allozymes, has involved pattern analysis for individual alleles, but new PCR-based genetic markers are coming into vogue, with vastly increased numbers of alleles. The field is badly in need of an explicitly multivariate approach to autocorrelation analysis, and our purpose here is to introduce a new approach that is applicable to multiallelic codominant, multilocus arrays. The procedure treats the genetic data set as a whole, strengthening the spatial signal and reducing the stochastic (allele-to-allele, and locus-to-locus) noise. We (i) develop a very general multivariate method, based on genetic distance methods, (ii) illustrate it for multiallelic codominant loci, and (iii) provide nonparametric permutational testing procedures for the full correlogram. We illustrate the new method with an example data set from the orchid Caladenia tentaculata, for which we show (iv) how the multivariate treatment compares with the single-allele treatment, (v) that intermediate frequency alleles from highly polymorphic loci perform well and rare alleles poorly, (vi) that a multilocus treatment provides clearer answers than separate single-locus treatments, and (vii) that weighting alleles differentially improves our resolution minimally. The results, though specific to Caladenia, offer encouragement for wider application.

Alleles↗

Tissue harmonic image analysis based on spatial covariance.

The van Cittert-Zernike theorem has been widely used to describe spatial covariance of the pressure field backscattered from a speckle object. Spatial covariance contains important information in the context of correlation-based correction of sound velocity inhomogeneities. Previous work was primarily based on spatial covariance analysis for linear imaging. In this paper, we extend the analysis to tissue harmonic imaging. Specifically, we investigate effects of the signal-to-noise ratio (SNR) and sound velocity inhomogeneities on spatial covariance. Results from tissue harmonic imaging are also compared with those from linear imaging. Both simulations and experiments are performed. At high SNRs, although both linear imaging and tissue harmonic imaging have spatial covariance functions close to theory, the spatial covariance of tissue harmonic imaging is consistently lower than that of linear imaging regardless of the presence of sound velocity inhomogeneities. At low SNRs, on the other hand, spatial covariance of tissue harmonic imaging is significantly affected. Because the tissue harmonic signal is much weaker than the linear counterpart, the low SNR reduces the accuracy of correlation-based estimation. It is concluded that the linear signal is more suitable for correlation-based correction of sound velocity inhomogeneities, despite the fact that tissue harmonic imaging generally has improved image quality over linear imaging.

Ultrasonography↗

Spatial transcriptomic analysis of mouse parathyroid gland cells expressing an activating variant of Gcm2.

Glial cells missing 2 (GCM2) is an essential transcription factor for the development of parathyroid glands. Germline GCM2 variants that repress or enhance transcriptional activity predispose a subset of patients to hypoparathyroidism or hyperparathyroidism, respectively. A recurrent germline heterozygous activating missense variant of GCM2, p.Y394S has been identified in some patients with primary hyperparathyroidism. A genetically engineered knock-in mouse model of this variant corresponding to p.Y392S in the mouse Gcm2 gene (Gcm2 +/Y392S) did not show obvious parathyroid tumors. However, in GCM2-binding site mediated luciferase reporter assays in HEK293 cells, the mouse and the human variant both exhibited enhanced transcriptional activity. Therefore, we assessed the effect of this variant on gene expression in vivo in parathyroid glands from Gcm2 +/Y392S and WT mice. Using the 10x Genomics Visium platform, spatially resolved transcriptomic analysis was performed on formalin-fixed and paraffin-embedded (FFPE) tracheal tissue sections of Gcm2 +/Y392S and WT mice to capture RNA from parathyroid glands together with other cell types in the tissue sections. Transcriptome sequence data analysis detected 8 different clusters in the tissue sections based on similarity of gene expression profiles. Cluster-1, which contained parathyroid gland cells expressing Pth and Gcm2, was further evaluated for transcripts that were differentially expressed more than 2-fold in Gcm2 +/Y392S compared to WT. Increased transcript level of Lgals3 (galectin-3) was seen in Gcm2 +/Y392S parathyroid gland cells which is among markers of parathyroid carcinoma. Galectin-3 protein was detected in available FFPE human parathyroid samples of patients with germline heterozygous activating GCM2 variants, p.Y394S (n = 4/10) or p.L379Q (n = 2/2). These results indicate a potential for growth and malignancy of parathyroid glands expressing GCM2 variants. The transcriptomic data of mouse parathyroid gland cells generated in this study can serve as a valuable resource for investigating genes and pathways in normal or abnormal parathyroid gland growth and physiology.

GCM2, gene↗

Component analysis of spatial and spectral patterns in multispectral images. I. Basis.

A new (to our knowledge) theory of component pattern analysis in multispectral images is developed by using the methods of principal component analysis and nonlinear optimization with a nonnegativity constraint. Given images of a scene in different color bands, we estimate both the spectral curves of components included in the image and the spatial pattern corresponding to each spectral curve. In this method, neither spatial nor spectral features of the components are necessary, but the physical rule of nonnegative absorptivity and density nonnegativity is used for any material of any optical frequency at any position in the image. Experimental results of component analysis with real microscopic image data are shown to demonstrate the effectiveness of the proposed method.

Form Perception↗

Sex differences in the lateralization of spatial abilities: a spatial component analysis of extreme group scores.

Sex differences in the cerebral lateralization of two discrete components of spatial processing were investigated in high and low ability males and females using the dual-task paradigm. In the first phase of the experiment, the results indicated a pattern of right hemispheric control for a spatial visualization component, regardless of sex and ability level. In the processing of the spatial orientation component of spatial ability, high ability males and females showed left hemispheric lateralization, whereas low ability males and females displayed right hemispheric control. In the second phase of this study, it was observed that high ability females and low ability males may use a verbal mediation strategy in processing spatial visualization tasks. No verbal mediation effects were found for the spatial orientation component.

Adult↗

Spatial spectrum analysis of wave-front correction with a segmented mirror.

An expression is derived for the spatial power spectrum of wave-front errors after correction with a segmented mirror. This includes estimates of the spectral contributions of segment piston and tilt corrections and spatial aliasing by a regular array of segments. The approach allows rapid computation of wave-front error spectra in systems with highly segmented mirrors.

Journal Article↗

Spatially resolved analysis of small particles by confocal Raman microscopy: depth profiling and optical trapping.

Raman microscopy is a powerful method to provide spatially resolved information about the chemical composition of materials. With confocal collection optics, the method is well suited to the analysis of small particles, either resting on a surface or optically trapped at a laser focus, where the confocal collection volume optimizes the signal from the particle. In this work, the sensitivity and spatial selectivity of detecting Raman scattering from single particles was determined as a function of particle size. An inverted confocal Raman microscope was used to acquire spectra of individual surface-bound and optically trapped polystyrene particles with sizes ranging between 200 nm and 10 microm. The particles are in contact with aqueous solution containing perchlorate ion that served as a solution-phase Raman-active probe to detect interferences from the surrounding medium. The collection volume is scanned through single particles that are attached to the surface of the coverslip, and the sensitivity and selectivity of detection are measured versus particle size. The results compare favorably with a theoretical analysis of the excitation profile and confocal collection efficiency integrated over the volumes of the spherical particles and the surrounding solution. This analysis was also applied to the detection of particles that are optically trapped and levitated above the surface of the coverslip. The results are consistent with the optical trapping of particles at or near the excitation beam focus, which optimizes excitation and selective collection of Raman scattering from the particle.

Journal Article↗

High reliability rates of spatial pattern analysis by vectorcardiogram in assessing the severity of eccentric left ventricular hypertrophy.

In 33 patients, including 12 control subjects and 21 with eccentric LVH, LV mass determined by angiocardiogram was correlated to 26 VCG measurements (Frank system) calculated from the scalar X, Y, and Z leads. The results demonstrated that the most reliable indices of VCG in assessing the severity of eccentric LVH determined by angiocardiogram were the magnitude of the spatial mean QRS vector and the time of the spatial maximal QRS vector ("spatial VAT"), of which correlation coefficients were 0.93 and 0.93, respectively. Such high correlation coefficients have never been obtained with the usual ECG analysis. These findings strongly suggest that (1) increased QRS voltage and usual prolonged QRS duration in eccentric LVH are due to an increase in LV mass, and (2) prolonged VAT observed in eccentric LVH is closely related to an anatomic alteration, namely, the greater distance of intra-ventricular conducting pathways as the result of LV dilatation, as an increase in LV mass is usually paralleled by the grade of the chamber enlargement in this type of LVH. Regarding the T loop, correlations between the LV mass and the VCG measurements were less as compared to those of the QRS loop. In general, T changes in moderate or severe LVH may be also related to a certain altered cardiac muscle state, in addition to an increase in LV mass. Angiocardiographic and light microscopic findings of a patient with eccentric LVH in whom a widened QRS-T angle was demonstrated to an extent much more than that expected with an increase in LV mass are presented and discussed. The spatial pattern analysis by VCG is very useful and reliable in assessing the severity of eccentric LVH.

Adolescent↗

Spatial kinematic analysis of the upper extremity using a biplanar videotaping method.

A biplanar videotaping system is used to generate spatial kinematic data of an upper extremity motion. The technique is based upon the characterization of each segment by four points in three-dimensional space using biplanar videotaping and subsequent analysis by computer-aided descriptive geometry. The tests were conducted to determine the system's accuracy and repeatability. The results of the joint kinematics of the test subjects performing a diagonal reaching activity with and without an orthosis (or an assistive device) are presented.

Biomechanical Phenomena↗

A preliminary report on the role of spatial frequency analysis in the perception of breast cancers missed at mammography screening.

RATIONALE AND OBJECTIVES: Because several factors are involved in cancer detection, a malignant lesion that is visible on a mammogram will not necessarily be reported by the radiologist reading the case. Indeed, a significant fraction of screening-detected cancers are visible in retrospect, and were perceived by the radiologist when the case was read, but were either reported as benign findings or dismissed as variations of normal breast tissue. In this preliminary report the spatial frequency characteristics of clinically missed lesions are investigated by analyzing the mammogram acquired when the lesion was sent for biopsy and the most recent prior mammogram. For control purposes, the contralateral breast is also analyzed, when this breast is lesion free. MATERIALS AND METHODS: A database of 70 mammogram cases was assembled. Each case contained eight films: craniocaudal (CC) and mediolateral oblique (MLO) of the breast where a biopsy-proven lesion was found, CC and MLO of the contralateral breast, and CC and MLO of both breasts in the most recent prior mammogram. The dictated reports for all of these cases were obtained. Both benign and malignant lesions were used. The films were digitized and an region of interest surrounding each lesion was segmented from the image for processing using wavelet packets to extract spatial frequency information. The corresponding area was also segmented from the prior mammogram and from the contralateral breast, when this breast was lesion-free. Analysis of variance was used to determine if statistically significant differences existed between the derived features of cancer in the current and prior mammograms. RESULTS: The data suggests that malignant lesions reported in the prior mammogram as being benign differed from correctly reported malignant lesions and from correctly reported benign lesions. They also differed from nonreported malignant lesions. In addition, the spatial frequency representation of cancer significantly differed in the current and prior cases from the representation of normal breast tissue. CONCLUSION: Spatial frequency analysis may be useful to differentiate malignant lesions that are reported as benign and correctly reported benign lesions.

Algorithms↗

Spatial and temporal analysis of the local response to wounding in Arabidopsis leaves.

We studied the local response to wounding in Arabidopsis thaliana leaves using a two-step microarray analysis. A microarray containing 3500 cDNA clones was first screened to enrich for genes affected by wounding in the immediate vicinity of the wound (4 h post wounding). 359 non-redundant putative wound responsive genes were then spotted on a smaller wound-response array for detailed analysis of spatial expression (local, adjacent and systemic), timing of expression (0.5, 4, 8, 17 h), and effect of hormone treatments (methyl jasmonate, ethylene and abscisic acid). Our results show that genes that respond early at the site of the wound also respond throughout the plant, with similar kinetics. Early-induced genes which respond systemically encode predominantly signal transduction and regulatory factors (36%), and the expression of many of them is also controlled by methyl jasmonate (about 35% of the 36%). Genes specific to the wound site and the wounded leaf have a slower response to wounding and are mainly metabolic genes. At the wound, many genes of the lignin biosynthesis pathway were induced. In silico analysis of the 5' promoter regions of genes affected by wounding revealed G-box-related motifs in a significant proportion of the promoters. These results show that the establishment of a systemic response to wounding is a priority for the plant, and that the local response at the wound site is established later. Ethylene and abscisic acid are involved in the local response, regulating repression of photosynthetic genes and expression of drought responsive genes respectively.

Abscisic Acid↗

APOE polymorphism distribution among Native Americans and related populations.

BACKGROUND: Apolipoprotein E (apoE, protein; APOE, gene) plays a central role in lipid metabolism. Three common alleles, E*2, E*3 and E*4 have quantitative effects on lipid and lipoproteins levels, which are major risk determinants of cardiovascular diseases in several populations. Given their clinical significance, it is of interest to know the distribution of APOE variants in populations from diverse ethnic groups, as well as to determine if this polymorphism presents variations that might be associated with given evolutionary factors. AIM: We report the distribution of APOE polymorphisms in Native American populations from South America, comparing it with other native populations of the Americas and Siberia. SUBJECTS AND METHODS: The sample consisted of 315 individuals from nine Native American populations living at subtropical latitudes of Argentina, Brazil and Paraguay. The extended analysis included 50 populations across South and North America, Greenland and Siberia. The geographic patterns of the variation were investigated through correlation analysis, spatial autocorrelation and analysis molecular of variance (AMOVA). RESULTS: The incidence of the most common allele (APOE*3) in the sample analysed ranged from 0.78 to 0.98. The second allele in prevalence, APOE*4, varied from 0.00 to 0.17. The rare allele APOE*2 was found in five of the nine populations investigated. This variant was found in a male with both maternal and paternal Native American lineages, suggesting that this allele is present in Native Americans and hence should not be used as an indicator of admixture. APOE*3 and APOE*4 present, respectively, positive and negative associations with latitude, although the pattern is much more pronounced in the Northern Hemisphere than in South America. APOE*2 increases its frequency with latitude but this pattern is statistically significant only in South America. CONCLUSION: The overall APOE spatial pattern seems, in general, compatible with a directional demographic expansion which occurred in north-eastern Asia and much of the New World. The APOE*2 allele shows this pattern in South America but a random distribution in the Northern Hemisphere, suggesting that the possibility of selection should not be discarded.

Apolipoproteins E↗

Analysis of spatial patterns of phase in neocortical gamma EEGs in rabbit.

Arrays of 64 electrodes (8 x 8, 7 x 7 mm) were implanted epidurally on the surface of the visual, auditory or somatosensory cortex of rabbits trained to discriminate conditioned stimuli in the corresponding modality. The 64 electroencephalographic (EEG) traces at all times displayed a high degree of spatial coherence in wave form, averaging >90% of the variance in the largest principal components analysis component. The EEGs were decomposed with the fast Fourier transform (FFT) to give the spatial distributions of amplitude and phase modulation (AM and PM) in segments 128 ms in duration. Spatial (2-dimensional) and temporal (1-dimensional) filters were designed to optimize classification of the spatial AM patterns in the gamma range (20-80 Hz) with respect to discriminative conditioned stimuli. No evidence was found for stimulus-dependent classification of the spatial PM patterns. Instead some spatial PM distributions conformed to the pattern of a cone. The location and sign (maximal lead or lag) of the conic apex varied randomly with each recurrence. The slope of the phase gradient varied in a range corresponding to that of the conduction velocities reported of axons to extend parallel to the cortical surfaces. The durations and times of recurrence of the phase cones corresponded to those of the optimally classified spatial AM patterns. The interpretation is advanced that the phase cones are manifestations of state transitions in the mesoscopic dynamics of sensory cortices by which the intermittent AM patterns are formed. The phase cones show that the gamma EEG spatial coherence is not due to volume conduction from a single deep-lying dipole generator nor to activity at the site of the reference lead on monopolar recording. The random variation of the apical sign shows that gamma AM patterns are self-organized and are not imposed by thalamic pacemakers. The half-power radius of the phase gradient provides a useful measure of the soft boundary condition for the formation and read-out of cooperative cortical domains responsible for binding sensory information into the context of prior experience in the process of perception.

Animals↗