Rhodotorula glutinius in the drain of haemodialysis machines.
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The accumulation of microorganisms embedded in biofilm within the drainage pipework leading from individual dialysis monitors in a renal dialysis centre, represents a significant threat to the safe operation of the whole centre due to blockage of the pipes and overflow of waste water. Attempts to disperse the growth with chemicals and disinfectants have been unsuccessful. Only mechanical rodding has removed the deposit, and regrowth has occurred. Those planning new dialysis centres should ensure that effluent pipework is readily accessible with multiple rodding eyes and is made of material able to withstand rodding and chemicals.
In order to study the long-term distribution and population dynamics of Pseudomonas aeruginosa strains in a highly contaminated hospital environment, two 4-week epidemiological studies, with an interval of 4 years, were carried out in the cystic fibrosis (CF) ward of the Paediatric Clinic of the Medical School of Hannover. Out of the 1948 specimens taken, P. aeruginosa was mainly identified in those from moist, inanimate sources (200 isolates) and hospitalized CF patients (168 isolates). A correlation was established between the frequency with which P. aeruginosa-positive patients came into contact with hospital facilities and the rate of contamination of these facilities. Rooms reserved for colonized patients were more frequently contaminated with P. aeruginosa in contrast to function rooms in the same ward and the outpatient clinic. However, no direct exchange between patients' strains and the inanimate hospital environment was detected. Out of the 11 genotypes of P. aeruginosa found in 1989 and the 13 genotypes found in 1993, four genotypes were present on both occasions. The most predominant clone was found in tap-water, sinks, wash-basins and creams with an incidence of 34 and 68% in the environmental isolates. The strains seemed to have spread into the adjacent control ward during the 4-year interval. Thus, the separation of colonized and non-colonized patients was undermined through the transfer of strains from a highly contaminated environment without additional hygiene precautions.
This paper explores recent attempts to improve the effectiveness of environmental health programmes and projects by reference to the International Drinking Water Supply and Sanitation Decade (1980-1990) and beyond. Reference is made to how water and sanitation as technical interventions have drawn upon the natural sciences, notably concepts of race and sex, and the social sciences including culture and gender, for their authority and legitimacy. A new and apparently progressive movement, the Water Decade sought to challenge the powerful and enduring high tech image of development on which much western environmental and social transformations have been based. Beginning as a critique of modernism with a commitment to basic needs as human rights, it was driven by a recognition that sophisticated technology could not satisfy human health needs. Alternative technologies would, by contrast, cater for a more extensive and varied market and would promote participatory approaches to service delivery. The paper demonstrates how, during the course of the Decade, sections of the aid community began to redefine basic needs as commodities involving the efficient marketing and delivery of a product with minimal state intervention. Within a shifting international political and economic context, it examines the changing role of the expert and the links being forged between large donors, non-governmental organisations and the private sector. The significance of this reformulated progressivism for the development debate is then considered, notably in relation to concepts of citizenship, consumer choice and the role of the state.
Between October 1987 and March 1989, we tested 144 water samples obtained from the plumbing and cooling tower systems of 5 Paris hospitals for the presence of legionellae and amoebae. Of the samples tested for Legionella, 67 out of 144 (46.5%) were positive, and 82 out of 116 tested for amoebae (70.7%) were positive. The ability of protozoa to support the multiplication of legionella was shown by incubating samples at 35.5 degrees C for 7-15 days. Prior to determining the presence of legionellae and amoebae, 51 of the 144 samples were incubated. After incubation, 22 out of 25 (88%) samples which were positive for the presence of both Legionella and amoebae showed multiplication of Legionella. In 3 out of the 25 (12%) samples containing Legionella and amoebae, Legionella failed to multiply. Six out of the 51 (11.8%) samples which were negative in direct culture for Legionella but positive for amoebae, became positive after incubation. Legionella did not multiply in samples negative for amoebae, nor was there proliferation in samples after filtration through a 1.2-microns membrane followed by incubation for the same period and temperature. Strains of Legionella pneumophila serogroup 1 and serogroup 6 (SG1 and SG6), including 3 patient isolates and 2 environmental isolates, were cocultured with 2 strains of amoebae and Tetrahymena pyriformis. Plate counts, Gimenez staining and electron microscopy demonstrated that intracellular legionellae proliferation occurred.
A laboratory experiment was conducted to determine the release of boron from soil-sewage sludge mixtures by leaching using a clinoptilolite type natural zeolite, before land application of the sewage sludge. Soil columns were filled up with the clinoptilolite soil after mixing with sewage sludge at a rate of 30 tons ha(-1) and with two different particle sizes (0.1-0.25 and 1.0-2.0 mm) of clinoptilolite each at the concentrations of 1% and 2%. The particle size and the application rate of clinoptilolite affected both boron leaching from soil compared to the control treatment (soil and sewage sludge mixture). The total soluble boron leached from a soil column varied from 66-92% depending on the applications of clinoptilolite and reached 96% for the control treatment, following application of 80 cm depth of water in all treatments. In the cases of the 1% application rate of 0.1-0.25 and 1-2 mm sized clinoptilolite 78% and 92% of the total boron leached, respectively. While at 2% application rate of 0.1-0.25 and 1-2 mm zeolite, 66% and 87% of total soluble boron leached, respectively. Boron concentrations in the soil layers increased as application rate increased and particle size of clinoptilolite decreased because of its high adsorption capacity. Adsorption isotherms indicated that clinoptilolite had a high adsorption capacity for boron compared to the sewage sludge and soil.
Overall food and water safety can be managed effectively through careful analysis of risks; implementation of site-specific food and water safety programs, including HACCP systems; careful assignment of responsibilities for implementation; and follow-up evaluation of effectiveness.
An approach for quantifying the human health risk caused by industrial sources, which, daily or accidentally, emit dangerous pollutants able to impact on different environmental media, is introduced. The approach is performed by the HHRA-GIS tool which employs an integrated, multimedia, multi-exposure pathways and multi-receptors risk assessment model able to manage all the steps of the analysis in a georeferenced structure. Upper-bound excess lifetime cancer risk and noncarcinogenic hazards are the risk measures, the spatial distribution of which is calculated and mapped on the involved territory, once all the pathways and receptors of the study area are identified. A sensitivity analysis completes the calculations allowing to understand how risk estimates are dependent on variability in the factors contributing to risk. The last part of the paper makes use of a case study concerning a working industrial site to put in evidence in which way the designed tool can help local authorities and policy makers in managing risks and planning remedial and reduction actions. The considered geographical area is a hypothetical territory characterized by residential, agricultural and industrial zones. The presence of two sources of contamination, a municipal waste incinerator (MWI) and a contaminated site, are evaluated by the tool application. Various typologies of receptors have been taken into account, each of them characterized by different anatomical and dietary properties. The achieved results are analyzed, compared with acceptable and background values and alternatives of minor environmental impact calculated.
Scales and sludge generated during oil extraction and production can contain uranium, thorium, radium and other natural radionuclides, which can cause exposure of maintenance personnel. This work shows how the oil content can influence the results of measurements of radionuclide concentration in scale and sludge. Samples were taken from a PETROBRAS unit in Northeast Brazil. They were collected directly from the inner surface of water pipes or from barrels stored in the waste storage area of the E&P unit. The oil was separated from the solids with a Soxhlet extractor by using aguarras at 90+/-5 degrees C as solvent. Concentrations of 226Ra and 228Ra in the samples were determined before and after oil extraction by using an HPGe gamma spectrometric system. The results showed an increase in the radionuclide concentration in the solid (dry) phase, indicating that the above radionuclides concentrate mostly in the solid material.
Infection control input is vital throughout the planning, design and building stages of a new hospital project, and must continue through the commissioning (and decommissioning) process, evaluation and putting the facility into full clinical service. Many hospitals continue to experience problems months or years after occupying the new premises; some of these could have been avoided by infection control involvement earlier in the project. The importance of infection control must be recognized by the chief executive of the hospital trust and project teams overseeing the development. Clinical user groups and contractors must also be made aware of infection control issues. It is vital that good working relationships are built up between the infection control team (ICT) and all these parties. ICTs need the authority to influence the process. This may require their specific recognition by the Private Finance Initiative National Unit, the Department of Health or other relevant authorities. ICTs need training in how to read design plans, how to write effective specifications, and in other areas with which they may be unfamiliar. The importance of documentation and record keeping is paramount. External or independent validation of processes should be available, particularly in commissioning processes. Building design in relation to infection control needs stricter national regulations, allowing ICTs to focus on more local usage issues. Further research is needed to provide evidence regarding the relationship between building design and the prevalence of infection.
Legionnaires' disease is one of the major infectious risks related to hospital water systems. It is commonly accepted, that the disease is transmitted to man mostly by inhalation of water aerosols contaminated by Legionella pneumophila. The ability of L. pneumophila to multiply intracellularly within some amoebae better explains the ecology, the pathogenicity, and the virulence of this bacterium against human alveolar macrophages. The presence of these amoebae in water systems located where cases of Legionnaire's disease broke out, partly explains the difficulty in eradicating Legionella. Some studies also show that amoebae can play a major role in the transmission of the disease to man. Some other studies point out that inhaled amoebae could be involved in the pathogenesis of Legionnaire's disease. Future strategies to prevent the transmission of Legionella will probably have to include efficient treatments against amoebae.
There is a large variety of options available for the effective treatment of odorous emissions. The most important physical, chemical and biological treatment processes are shortly described and their favourable applications, as well as their limits, are highlighted. But for a sustainable solution of an industrial odour problem, there is more involved than just the installation of a waste gas treatment system. This article focuses on a general and systematic approach towards extensive odour management. First of all, an odour assessment should be worked out where all actual and potential odour emission sources are recorded and characterised. A special focus should be set on fugitive emissions, which may have an enormous impact on the overall odour problem. They need to be captured before they can be supplied to a treatment system. According to the composition and condition of the waste gases, an appropriate treatment system must be selected. For this purpose, test systems have been developed and are presented in this article.
Absorption is one of the most widely used techniques for treating odourous waste gases. An improvement of the effectiveness of gas scrubbers can be achieved by using adapted washing liquids. A screening test is proposed as a low cost tool for testing washing liquids on industrial waste gases. The odour index is used to identify the main cause of the specific smell and to simplify the analysis of absorptive performances of washing liquids. The method is verified by experiments at a chocolate factory and a fat and oil refinery.
The quest for simple, low-cost and high-performance decentralized wastewater treatment system for domestic application in developing nations necessitated this study. Clay samples collected from different deposits in Nigeria were characterized by studying the mineralogical and geochemical composition using X-ray diffraction (XRD) and atomic absorption spectroscopy (AAS), respectively. Three major clay minerals of kaolinite, illite and smectite were identified. The geochemical studies showed the abundance of SiO2, Al2O3 and H2O+ in each of the clay samples. Performance efficiency studies were conducted to determine the best combination ratio of pebbles/soil-clay. Soil-clay fortified by pebbles in combination ratios of 1:3 (i.e. pebbles:soil-clay = 1:3 (w/w) showed the optimum water purification, while the combination 3:1 gave the least. The flow rate studies showed that the wastewater had a longer residence time in non-fortified soil-clay than in fortified soil-clay. Two modes of treatment methods were employed-single and double column treatment methods (SCT and DCT). The two methods gave effluents of good quality characteristics, but those from the DCT were of better quality. The quality of effluents also varies from one clay type to another. The quality of effluents from media containing smectite clay mineral was better than those from other columns. Repeated usage of the fortified clay column showed a decrease of pH, TS and DO, and an increase of COD when monitored over a period of 10 days.
As part of achieving lower lead standards water undertakers are utilising lead pipe rigs to quantify the benefit of treatment measures. A convective diffusion model is developed for lead pipe rigs operating in laminar flow, and applied to the three operating steps of flushing, sampling and stagnation. The model is used to determine the appropriate time-scales for each stage, and the sensitivity of the measure to variations in flow-rate. In contrast to rigs operating in turbulent flow the average lead observed leaving the pipe and that in the pipe, after a period of stagnation, are substantially different. Equations are derived for both, and take into account the residual distribution of lead left in the pipe after flushing. It is shown that the lead concentration observed leaving the pipe is well approximated by a single exponential term in contrast to the concentration within the pipe. Predictions are made on the residual lead concentration that can be achieved through flushing, and its dependence on flow-rate. The relevance of the laminar flow model to that in domestic lead pipes is discussed.
We studied the effects of flow velocity on the formation of biofilms and the concentration of bacteria in water in copper and plastic (polyethylene, PE) pipes. The formation of biofilms increased with the flow velocity of water. The increase in microbial numbers and contents of ATP was clearer in the PE pipes than in the copper pipes. This was also seen as increased consumption of microbial nutrients in the pipeline system. This indicates that the mass transfer of nutrients is in major role in the growth of biofilms. However, the increased biomass of biofilms did not affect microbial numbers in the water. Rapid changes in water flow rate resuspended biofilms and sediments which increased the concentrations of bacteria and copper in water. The disturbance caused by the changing water flow was also seen as an increase in the particle counts and water turbidity recorded with online instrumentation.
Water quality can deteriorate in the transmission and distribution system beyond the treatment plant. Minimizing the potential for biological regrowth can be attained by chlorinating the finished water. While flowing through pipes, the chlorine concentration decreases for different reasons. Reaction with the pipe material itself and the reaction with both the biofilm and tubercles formed on the pipe wall are known as pipe wall demand, which may vary with pipe parameters. The aim of this paper was to assess the impact of the service age of pipes on the effective chlorine wall decay constant. Three hundred and two pipe sections of different sizes and eight different pipe materials were collected and tested for their chlorine first-order wall decay constants. The results showed that pipe service age was an important factor that must not be ignored in some pipes such as cast iron, steel, cement-lined ductile iron (CLDI), and cement-lined cast iron (CLCI) pipes especially when the bulk decay is not significant relative to the wall decay. For the range of the 55 years of pipe service age used in this study, effective wall decay constants ranged from a decrease by -92% to an increase by +431% from the corresponding values in the recently installed pipes. The effect of service age on the effective wall decay constants was most evident in cast iron pipes, whereas steel pipes were less affected. Effective chlorine wall decay for CLCI and CLDI pipes was less affected by service age as compared to steel and cast iron pipes. Chlorine wall decay constants for PVC, uPVC, and polyethylene pipes were affected negatively by pipe service age and such effect was relatively small.
Simple models based on the physical and biochemical processes occurring in septic tanks, pit and urine diversion latrines were developed to determine the nutrient flows in these systems. Nitrogen and phosphorus separation in different output materials from these on-site sanitation installations were thus determined. Moreover, nutrient separation in septic tanks was also assessed through literature values and by eliciting expert judgement. Use of formal expert elicitation technique proved to be effective, particularly in the context of developing countries where data is often scarce but expert judgement readily available. In Vietnam, only 5-14% and 11-27% of the nitrogen and phosphorus input, respectively, are removed from septic tanks with the faecal sludge. The remaining fraction leaves the tank via the liquid effluent. Unlike septic tanks, urine diversion latrines allow to immobilize most of the nutrients either in form of stored urine or dehydrated faecal matter. These latrines thus contribute to reducing the nutrient load in the environment and lowering consumption of energy and non-renewable resources for fertiliser production.