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PubMed2000

Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population.

The occurrence of Enterococcus faecalis in root canals of previously root filled teeth with apical periodontitis requiring retreatment was studied in Lithuanian patients. Twenty-five asymptomatic teeth were included in the study. Avoiding contamination microbiological samples were taken from the canals before and after preparation and irrigation with sodium hypochlorite and EDTA. Microbes were isolated from 20 of 25 teeth. E. faecalis was isolated from 14 of those 20 culture positive teeth, usually in pure culture or as a major component of the flora. Second samples taken after preparation revealed growth in 7 of the 20 teeth. Five of the seven cases were E. faecalis in pure culture. Isolation of E. faecalis was not related to the use of any particular root filling material in the original root filling. The results indicate that, rather than previous chemical treatment, it is the ecological conditions present in the incompletely filled root canal that are important for the presence of E. faecalis in these teeth.

Bacteriological Techniques↗
PubMed2001

An evaluation of endodontically treated vertical root fractured teeth: impact of operative procedures.

Vertical root fractures of endodontically treated teeth are a frustrating complication that leads to extraction. The aim of the current survey was to evaluate the role of operative procedures in the etiology of this complication. A total of 154 endodontically treated vertical root fractured teeth were cleaned and washed after extraction and maintained in individual vials. Periapical radiographs before extraction, clinical findings and previous operative procedures were recorded. A post was observed in 95 teeth (61.7%), with 66 of these ending at the coronal third of the root. Most were screw posts of the Dentatus type (n = 64) and tapered cast posts (n = 14). A full crown was observed in 118 teeth, and 65 of these (55%) were extracted between 1 to 5 yr after final restoration. In 24 crowned teeth extraction was conducted within 1 yr after restoration and in 28 teeth after >5 years. It was concluded that post placement and root canal treatment are the major etiological factors for root fractures. Because signs and symptoms can appear years after the operative procedures in the root have been completed, coronal restorations would not interfere with the correct clinical diagnosis of vertical root fractures. Frequent recalls are recommended to diagnose vertical root fractures early, especially in susceptible teeth, such as premolars and mesial roots of mandibular molars.

Bicuspid↗
PubMed2001

Effect of removal of the smear layer on apical microleakage.

It has been shown previously that the smear layer created during instrumentation of the root canal promotes apical microleakage. The purpose of the present study was to investigate the effect of removal of the smear layer on apical microleakage, using the fluid filtration method. Thirty-six single-rooted human premolar teeth were used. Six of the teeth were selected randomly as controls, and the remaining 30 teeth were randomly divided into two groups of 15 teeth each. For the first group (group 1) teeth were irrigated with NaOCl only (smear layer not removed). In the second group (group 2) the teeth were irrigated with 15% EDTA, followed by NaOCl to remove the smear layer. Both groups were filled with thermoplasticized gutta-percha (Obtura II) using glass-ionomer cement as sealer. All teeth were stored at 37 degrees C and 100% humidity for 2 days. Apical microleakage of the filled root canal was measured by the fluid filtration technique. Hydraulic conductance was measured by the movement of an air bubble in a capillary tube after a pressure of 200 mm Hg was applied to the system. The mean microleakage rate of group 1 and group 2 were 13.0 (SD +/- 5.1) and 16.9 (SD +/- 15.3) nl/s, respectively. Removal of the smear layer caused significantly more apical microleakage (p < 0.05, Student's t test) than when the smear layer was left intact.

Bicuspid↗
PubMed2001

Setting times for endodontic sealers under clinical usage and in vitro conditions.

The object of this study was to determine the set of sealers under simulated clinical versus bench-top conditions. One hundred twenty extracted teeth were divided into four groups (Roth's, Tubuliseal, Sealapex, AH26). Canal preparation and lateral condensation were performed with the four sealers (30 teeth each). Teeth were placed into 100% humidity at 37 degrees C. The same mix of sealer on a glass slab was stored within the same environment. After 1 to 8 wk the teeth were fractured longitudinally to expose the gutta-percha-sealer interface. Evaluation, both clinical and in vitro, was qualitative under a microscope with a needle tip. The degree of set was categorized as: (a) unset (smearable), (b) partially set (indented easily, but not smearable), or (3) set (not easily indentable). There were inconsistencies in setting times within and between groups. In canals most of three (AH26, Sealapex, and Tubuliseal) were partially set after 1 wk; set was complete after 4 wk. Roth's was very slow; most were unset after 8 wk. Sealers on the glass slab set much more rapidly. In conclusion, under clinical conditions, sealers set slowly (particularly Roth's) and were more delayed than when tested in vitro.

Bismuth↗
PubMed2001

In vitro apical leakage of system B compared with other filling techniques.

A fluid filtration system was used to compare the apical microleakage of roots filled with the system B (Analytic Technology), single-cone technique, lateral condensation, vertical condensation, and Thermafil (Caulk-Dentsply). After preparation of the canal system with a Profile (Caulk-Dentsply) five groups of 10 single-rooted teeth were randomly filled according to 1 of the 5 techniques. Phosphate-buffered saline was forced under a 15 cm H2O pressure, through the apex, toward the coronal part of the root. The filtration rate in L s(-1) Kpa(-1) was recorded 24 h after filling and after 1-month storage in phosphate-buffered saline. The 24 h results showed that the single cone technique provided the highest leakage (p = 0.001). At 1-month system B, Thermafil and vertical condensation had less leakage than the two other techniques (p = 0.0001): lateral condensation produced moderate apical leakage, whereas the single cone technique showed the highest leakage. Regardless of the technique the apical leakage increased after 1-month storage (p = 0.001 or p = 0.0001, depending on the technique).

Analysis of Variance↗
PubMed2002

Comparison of two ultrasonic instruments for post removal.

The relative performance of two different ultrasonic units commonly used clinically for post removal was evaluated using tips designed specifically for post vibration. Twenty-four extracted maxillary and mandibular cuspids with crowns removed at the labial cementoenamel junction were treated endodontically. Post spaces were made 10 mm into the roots before cementing a 16 mm #5 (0.050-inch) Para-Post with zinc phosphate cement. The teeth were divided into three similar groups of eight. Post retention was assessed in group 1. Ultrasonic vibration was applied to groups 2 and 3 until post removal. The average force required to dislodge the posts from the teeth in group 1 (control group, no ultrasound) was 40.5 kg (SD = 12.3 kg). The average time for post removal in group 2 (Spartan) was 4:52 min (SD = 2:26). The average time for post removal in group 3 (Enac) was 1:31 min (SD = 0:34). The difference between groups 2 and 3 was statistically significant (p < 0.005). Use of ultrasonic tips designed for post vibration and maximization of audible sound level during ultrasonic treatment of posts seem to play an important role in the effectiveness and efficiency of post removal. The results obtained indicate that both the Enac ultrasonic unit with the ST-09 vibration tip and the Spartan ultrasonic unit with the Analytic VT-S tip were effective. Nevertheless, the Enac ultrasonic unit with the ST-09 vibration tip was clearly more efficient under these study conditions, resulting in typical post removal times of <2 min.

Cementation↗
PubMed2002

Evaluation of two post core systems using two different methods (fracture strength test and a finite elemental stress analysis).

The aim of this study was to compare a fiber composite laminate (FCL) post core and a conventional cast post core system by using two different methods. The first method was a conventional fracture strength test, and the second was a finite elemental stress-analysis method (FEM). For the conventional fracture strength test, 20 extracted, human upper, central incisors were used. The teeth were decoronated, treated endodontically, and restored with two post core systems. After embedding the samples in resin blocks, a loading force was applied to the teeth at a crosshead speed of 5 mm/ min at an angle of 45 degrees to the long axis of the tooth. The data were recorded, and the results were compared by using the Mann-Whitney U test. There was no statistically significant difference between the two post systems (p > 0.05). For FEM analysis, a pseudo three-dimensional model of a maxillary central incisor, theoretically restored with either a cast post or an FCL, was used. The analysis was performed by using the structural analysis program (SAP90). FEM analysis showed that stress was accumulated within the cast post core system, and transmission of stress to supportive structures and the tooth was low. This is an advantage for tooth and supporting tissues. When the FCL post core system was evaluated by FEM, the results indicated that this system transferred stress to supportive structures and the tooth while stress accumulation within the post system was low. This is an advantage for the restoration but disadvantage for the supporting tissues.

Computer Simulation↗
PubMed2002

Apical healing of an endodontically treated tooth with a temporary restoration.

A 35-yr-old, healthy male presented to the graduate endodontic clinic at Indiana University School of Dentistry for treatment of tooth #26. Two and one-half yr after treatment, the patient returned to the dental school for comprehensive treatment. The canal access opening had been restored with an intact interim restorative material restoration. The tooth was asymptomatic, and radiographic examination showed evidence of apical healing. In this article, a review of the literature concerning crown-down leakage is presented. A case report is given in which healing occurred after the tooth was restored with only an interim restorative material for an extended period of time.

Adult↗
PubMed2003

Cytotoxicity testing of endodontic sealers: a new method.

The purpose of this study was to compare ISO standards versus a new technique for in vitro evaluation of cytotoxicity of root canal sealers. The cytotoxicity of AH Plus, Cortisomol, and Sealapex was first recorded according to ISO standards on L 929 fibroblasts by the MTT assay. In parallel, 30 single-rooted teeth were cut at the cementum enamel junction (CEJ), and the roots were prepared and sterilized before filling with the lateral condensation using one of three sealers (n = 10). The apexes of the roots were dipped into 1 ml of minimum essential medium for 1, 2, and 30 days renewing the medium every other day. After 24-h contact between the medium and the filled roots, the medium was used to measure the cytotoxicity on L 929 with the MTT assay. ISO standards always gave a statistically higher cytotoxicity than the root-dipping technique (p < 0.0001), whatever the sealer and the exposure time. The ISO standards showed statistically significant differences among the sealers (p < 0.0001). AH Plus was noncytotoxic, Cortisomol showed a high cytotoxicity decreasing over time (p < 0.001), and Sealapex displayed a high cytotoxicity that did not decrease over time (NS). The new technique showed statistically significant differences among the sealers (p = 0.001), but the differences were so small that they were likely not clinically relevant. The high cytotoxicity of Sealapex decreased over time but the cytotoxicity of AH Plus and Cortisomol did not. The results show that the ISO standards may strongly over-evaluate the cytotoxicity of the endodontic sealers, emphasize the difference among the sealers, and may clinically correspond to a large overfilling. The new technique reduces the discrimination of the test and may clinically correspond to a classical filling. Therefore, both methods might be considered as clinically relevant, corresponding to classical and overfilling conditions.

Analysis of Variance↗
PubMed2003

Evaluation of an ultrasonic technique to remove fractured rotary nickel-titanium endodontic instruments from root canals: clinical cases.

The removal of fractured rotary nickel-titanium instruments from small, curved root canals is one of the most complex operative procedures in endodontics. There have been many different devices and techniques developed to remove fractured instruments, but none are consistently successful and all show a high incidence of canal damage such as perforation. A technique using a staging platform and the use of modern ultrasonic tips with direct visualization through a dental operating microscope has recently been evaluated. The use of this technique in clinical practice is described in 24 cases and illustrated by 3 cases.

Adult↗
PubMed2003

Treatment outcome in endodontics: the Toronto Study. Phase 1: initial treatment.

This study assessed the 4- to 6-yr outcome of initial endodontic treatment during Phase 1 of the "Toronto Study" project. A total of 450 teeth were treated, alternating the "Schilder" technique and step-back with lateral condensation, and examined clinically and radiographically by an independent examiner. Apical periodontitis was recorded as absent (periapical index < 3) or present (periapical index > or = 3) and outcome dichotomized as "healed" (no apical periodontitis, no signs or symptoms) or "disease." Univariate, bivariate, and multivariate analyses were performed (p = 0.05) on 120 examined teeth. The "healed" rate (81% overall) was significantly higher for teeth treated without apical periodontitis (92%) than with apical periodontitis (74%). Several other factors were associated with healing rate differential of 10% or higher, but no statistical significance. This study confirmed apical periodontitis as the main prognostic factor in initial endodontic treatment. Continuation of the project will allow assessment of other prognostic factors with better power.

Chi-Square Distribution↗
PubMed2004

Nonsurgical endodontic treatment of dens invaginatus with large periradicular lesion: a case report.

The endodontic treatment of teeth with severe Type 3 dens invaginatus, characterized by an infolding of enamel and dentin, extending deep into the pulp cavity near the root apex, may be complicated and challenging. Because of the bizarre root canal anatomy and widely open apex, a combination of nonsurgical and surgical endodontic treatment or extraction is the most common choice of therapy. This article describes a nonsurgical endodontic treatment of a tooth with severe Type 3 dens invaginatus and an associated large periradicular lesion. After complete removal of the invaginated central mass of hard tissue and long-term calcium hydroxide treatment, nonsurgical endodontic treatment was performed. Complete healing of the periradicular lesion was observed at 25-month and 74-month follow-up examinations.

Adult↗
PubMed2005

Root canal treatment in a mandibular second premolar with three root canals.

According to the endodontic literature, the possibility of three canals in mandibular second premolars is quite small. When three or more canals are present separation of the root canals has only been described at mid-root level. The present case describes root canal treatment of a root canal aberration at the level of the apical third (apical root canal separation starting at a length of 20 mm) where a combined use of the fiberoptic endoscope and the operating microscope was necessary to locate the canal orifices and to enter these orifices. The present report emphasizes the need of color contrast to enhance visualization.

Adult↗
PubMed2005

Interfacial strength of Resilon and gutta-percha to intraradicular dentin.

Strengthening of Resilon-filled roots via an adhesive interface should be reflected by improvement in the interfacial strength and dislocation resistance between the root fillings and intraradicular dentin. This study compared the interfacial strengths of Resilon/Epiphany and gutta-percha/AH Plus using a thin-slice push-out test design. Failure modes of root slices after push-out testing were examined with environmental scanning electron microscopy. The gutta-percha group exhibited significantly higher interfacial strength than the Resilon group, when premature failures that occurred in Resilon root slices were included in the statistical analysis. The gutta-percha root slices failed exclusively along the gutta-percha/sealer interface. The Resilon root slices failed predominantly along the sealer/dentin interface with recognizable, fractured resin tags. Detachment of the Resilon from the Epiphany sealer was also surprisingly observed in some specimens. The similarly low interfacial strengths achieved with both types of root filling challenges the concept of strengthening root-filled teeth with the new endodontic material.

Bismuth↗
PubMed2005

Apical leakage of resin based root canal sealers with a new computerized fluid filtration meter.

In this in vitro study, the apical leakage of three root-canal sealers: AH Plus, Diaket, and EndoREZ was evaluated using a new computerized fluid filtration meter. Forty-five extracted human premolar teeth with single root and canal were used. The coronal part of each tooth was removed and the root canals were prepared using GT Rotary files and crown-down technique. The roots were randomly divided into three groups of 15 samples, filled with one of the test materials and gutta-percha cones by the cold lateral condensation technique and were stored at 37 degrees C and 100% humidity for 7 days. One-week later, apical parts of roots of 10 +/- 0.05 mm were attached to computerized fluid filtration meter. Apical leakage quantity was determined as microl/cmH(2)O/min(-1). Statistical analysis indicated that root fillings with Diaket in combination with cold lateral condensation technique showed lower apical leakage than the others (p < 0.05). In addition, this new computerized fluid filtration meter allowed quantitative measurement of leakage easily. As it is a newly developed device to measure apical leakage of endodontic sealers, the reliability of it needed to be tested.

Bismuth↗
PubMed1990

Retrieval of silver cones using different techniques.

Three different techniques of silver cone retrieval were investigated in 41 teeth containing 91 silver cones. The techniques used for retrieval were as follows: (i) hand instrumentation with files, spoon excavators and forceps; (ii) the automated Canal-Finder-System; (iii) ultrasonic devices. In all teeth, these techniques were used in the same sequence, starting with hand instrumentation, followed by the Canal-Finder-System, and ending with ultrasonic devices. Fifty-two of the 91 cones (57 per cent) could be removed with hand instruments. Of the remaining 39 cones, 19 were removed after using the Canal-Finder-System. An additional 10 cones could be removed after the use of ultrasonics. Two cones could only be bypassed by the Canal-Finder-System but were not removed with any of the techniques investigated in this study. Eight silver cones (9 per cent) could neither be bypassed nor removed. It was concluded that silver cone retrieval in more than half the cases could be performed easily with hand instruments, particularly spoon excavators. The automated Canal-Finder-System and ultrasonics proved to be helpful devices in many difficult cases, but did not guarantee success in every case.

Humans↗