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At least 379 records · Page 21Linked to original sources
PubMed1989

[New diagnostic procedures in assessing male fertility].

In 160 patients with normal and pathological semen samples, we studied the penetration of human spermatozoa in standardized bovine cervical mucus (assay Penetrak). Our results indicate that Penetrak can detect those dysfunctions of sperm motility which cannot be diagnosed by conventional semen analysis - The toluidine blue-pyronine staining is an easy way to differentiate between spermatozoa within an hour. The reliability of this technique is comparable to that of conventional methods and therefore appropriate for routine diagnostics. - As case studies on 140 patients proved, the level of carnitine in the seminal plasm can be regarded as a parameter of epididymal function. In combination with the examination of FSH serum levels, this method may be helpful in the differential diagnosis of azoospermia due to obstruction, Sertoli-cell-only syndrome, or spermatogenic arrest. - In severe oligozoospermia, testicular biopsy using semithin sections may be of high diagnostic and prognostic value. In contrast to paraffin sections, this technique is particularly appropriate for the detection of cytological characteristics of germ cells. The type and number of pathological germ cells are decisive regarding the prognosis. Furthermore, a carcinoma in situ (CIS) can be definitely identified by means of semithin sections. - In a study on 2047 patients, we found seminoma cells in 15 cases (0.73%); 5 of these patients already had a solid seminoma in the testicular rete. Risk-patients are those showing oligozoospermia of less than 10 million spermatozoa per ml or azoospermia associated with unilaterally or bilaterally subnormal testicular volume. In these cases, the testicles are inconspicuous, both palpatorically and sonographifically.

Biopsy↗
PubMedDate not supplied

Immunoblastic lymphadenopathy with pulmonary lesions and positive sputum cytology.

A case of immunoblastic lymphadenopathy is presented. The patient's diagnosis was made from his clinical findings and lymph node pathology. Of particular note are long-standing pulmonary lesions of undetermined etiology and the exfoliation in sputum specimens of large numbers of immunoblasts identical to those seen in histologic sections of lymph node. Although other cases of immunoblastic lymphadenopathy with pulmonary findings have been published, 4, 5 only the histomorphology of these lesions has been described. This is the first reported case of immunoblastic lymphadenopathy with positive pulmonary cytology. Diagnosis of this condition requires histopathologic evaluation of diagnostic lymph nodes. Immunoblasts may be identified cytologically in sputum preparations and in this context are suggestive of but not diagnostic of this entity. The use of methyl green-pyronine stain to better differentiate immunoblasts is recommended.

Humans↗
PubMed1989

Herpetic cervicitis--a histo-morphologic study.

The purpose of this paper has been to find out the prevalence of herpetic cervicitis among routine surgical cervical biopsies, categorize the morphological changes and study their evolution. In a 51-month-period, 879 human uterine cervices were examined histologically, 19 percent (168) conforming to predetermined criteria for herpetic cervicitis. These criteria were typed as A through D starting with nuclear homogenization and passing through vesicle formation, bulla formation and finally ulceration. The type D lesion showed an agglomeration of Type A-C morphological changes occurring together, in addition to which focal submucosal haemorrhages were seen with severe congestion. The presence of the Herpes virus was confirmed with the Peroxidase-Antiperoxidase stain and the evolution of the bullae studied by means of the Alcian Blue-Periodic Acid Schiff stain (AB-PAS), Methyl Green-Pyronine (MG-P) stain and the Reticulum stain.

Cervix Uteri↗
PubMed1977

[Simultaneous determination of T-, B- and mixed rosette-forming lymphocytes in human peripheral blood].

Preliminary treatment of erythrocyte suspension with trypan blue solution did not affect their capacity to participate in the reaction of spontaneous rosette formation, but permitted to differentiate them in a mixture with other erythrocytes after staining with methyl-green-pyronine on a fixed slide. By using a mixture of such marked erythrocytes sensitized with antibodies and a complement, the presence in the peripheral blood of healthy people of a small lymphocyte subpopulation connecting these two types of indicator particles was demonstrated.

B-Lymphocytes↗
PubMed1988

Comparison between the DNA precipitation and alkali unwinding assays for detecting DNA strand breaks and cross-links.

A new DNA precipitation assay used together with the alkali unwinding assay may provide a rapid means of detecting DNA damage in addition to strand breaks based on the relative amount of damage measured by the two assays. X-rays, Adriamycin, 4-nitroquinoline-N-oxide, N-methyl-N'-nitrosoguanidine, bleomycin, RSU 1172, and five other drugs produced the same relative amount of strand breakage by using the DNA precipitation and alkali unwinding assays. However, strand breaks produced by the bifunctional alkylating agents bis(2-chloroethyl)nitrosourea, RSU 1069, and RSU 1131 were detected with greater efficiency by the DNA precipitation assay, while the unwinding assay measured more strand breaks than the precipitation assay after damage by the topoisomerase inhibitors VP-16 and VM-26 and the DNA-condensing agents acridine orange and pyronin Y. Based on the reported mechanisms of action of these drugs, and studies with known DNA cross-linking agents, it appears that in addition to DNA strand breaks, the alkali unwinding assay is more sensitive to interstrand than to DNA-protein cross-links, while the DNA precipitation assay can be used to detect both types of cross-links. While quantification of specific lesions is not possible with this approach, the concomitant use of these two assays may provide a rapid and simple method for screening genotoxic drugs for DNA damage, and may also help to differentiate between DNA lesions which include strand breaks, interstrand and protein cross-links, DNA-phosphate adducts, and DNA-drug precipitates.

Animals↗
PubMed1988

[The temporal organization of the complex nodule in the lymph node].

The aim of the work is to study rhythmic processes at tissue level in the lymph nodule and in the T-territory adjacent to it in order to reveal temporal interrelations in functioning the germinative center, crown and T-territory. The lymph nodule sections are stained with methylene green-pyronine. Small and middle lymphocytes, immunoblasts, plasmoblasts, immature and mature plasma cells, mitotic figures are taken into account. Spectral composition and rhythmic parameters are determined for each type of the cells in the ultradian, circadian and infradian ranges. Periods, approaching the circadian one, are revealed in the germinative centers for small lymphocytes and immature plasma cells. Practically, for all plasma cells the functional period near to 7 h is found; this attests the presence of the common synchronous rhythm driver. Phase difference of immunoblast, plasmablast, immature plasma cell fluctuation in the germinative centers makes it possible to suppose the time, necessary to transfer the immunoblast into the plasmablast (1.6-2.6 h) and the plasmablast into the immature plasma cell (3 h). Owing to the knowledge of the spectral composition of the rhythmic and parameters of certain components it is possible to approximate the total course of the process. Combination of fluctuations with various periods results in their recurrence in more prolonged time intervals.

Animals↗
PubMed1986

Analysis of hemopoietic lineage of accessory cells in the developing thymus of Xenopus laevis.

The developmental history of accessory cells in the thymus was studied by grafting hemopoietic stem cells into cytogenetically distinct frog embryos (diploid-2N or triploid-3N) before the establishment of circulation and overt differentiation and colonization of the thymus. The DNA content of cortical thymocytes and circulating erythrocytes was quantified by staining with propidium iodide and measuring the amount of red fluorescence emitted by individual nuclei with the use of flow cytometry. Accessory cells from thymic medulla were separated by incubating for 2 hr on glass slides. For comparison, the developmental history of peritoneal macrophages was examined as representative, myeloid-derived phagocytic cells. DNA content of adherent cells was quantified by staining with the DNA-specific Feulgen reaction and measuring light absorption of individual nuclei by microdensitometry. Thymic accessory cells were subdivided into phagocytic and nonphagocytic phenotypes on the basis of latex bead ingestion. Phagocytic cells in the thymus were usually nonspecific esterase positive and phenotypically resembled peritoneal macrophages. Nonphagocytic cells from the thymus were usually esterase negative and had a dendritic morphology characterized by branched cytoplasmic extensions. Nonphagocytic cells were positive for cytoplasmic RNA based on staining with methyl green-pyronin Y. Phagocytic cells from both the thymus and the peritoneal cavity had no levels of cytoplasmic RNA detectable by this method. Analysis of the embryonic derivation of thymic accessory cells, based on the proportion of cells carrying the cytogenetic marker, demonstrated that thymic lymphocytes and thymic accessory cells were a concordant pair of cells, distinct from myeloid-derived erythrocytes and possibly macrophages. These experiments provide circumstantial evidence suggesting thymocytes and thymic accessory cells could arise from a bipotential precursor that diverges into these separate lineages after colonization of the epithelial thymic rudiment during early development.

Animals↗
PubMed1985

Plasmacytoma with oncocytic changes. A case report.

In this report we will document a readily identifiable, morphologic variant of plasma cells in an extramedullary plasmacytoma. Ultrastructurally, the tumor cell cytoplasm was almost completely occupied by proliferating mitochondria. Localized areas of endoplasmic reticulum were, however, also noted. The light microscopic features of this tumor (i.e. foamy eosinophilic cytoplasm) and special staining (methyl green pyronin (MGP) characteristics were compatible with the fine-structural details of these cells. The usefulness of obtaining a clinical/pathological correlation for the behavior of this type of tumor is discussed.

Aged↗
PubMedDate not supplied

Cytologic diagnosis of multiple myeloma and macroglobulinemia in effusions.

The presence of effusion in association with multiple myeloma or macroglobulinemia is an unusual finding which generally occurs late in the course of the disease. Occasionally, cytoligic detection is a diagnostic problem. In both conditions, the fluid is characrerized by a high specific gravity and high protein content. In myeloma, plasma cells at varying stages of differentiation are present, while in macroglobulinemia atypical lymphocytes and plasma cells resembling those found in the blood and bone marrow are seen. Generally, the diagnosis can be readily made on the basis of clinical features, characteristics of the fluid and cytologic findings. In difficult cases, additional procedures helpful in establishing a definite diagnosis include methyl green pyronine stain, fluid electrophoresis and electron microscopy.

Adult↗
PubMed1975

A supravital cytodiagnostic stain for urinary sediments.

A mixture of aqueous solutions of National fast blue, a copper-phthalocyanine dye, and pyronin B, a red xanthene dye, when added to fresh urinary sediment, supravitally stains benign or malignant cells and the various types of casts and their inclusions. The stain facilitates identification of the formed elements and particularly aids in the differentiation of polymorphonuclear leukocytes from lymphocytes, histiocytes, plasma cells, and renal tubular cells. A variable staining of casts and their inclusions has been observed. Tumor cells may be recognized by nuclear abnormalities or, in case of hyperchromatic tendency, by a very rapid and early uptake of dye preceding that of the surrounding cells. The staining method is rapid and simple enough for routine urinalysis and screening procedures.

Carcinoma, Transitional Cell↗
PubMed1975

Cytological, cytochemical and immuno-fluorescence studies with Dugbe virus - a new Nigerian tick-borne virus.

Dugbe virus, a new tick-borne arbovirus from Nigeria, was propagated in continuous porcine kidney (PS) cells, and the cytopathology studied by various staining techniques such as May-Grunwald-Giemsa, methyl-green pyronine, Feulgen, and immuno-fluorescence. The gross cytopathology was slight and infected cells continued to undergo normal mitotic divisions. The outstanding cytopathologic feature was the presence of large basophilic intracytoplasmic inclusion bodies, which were pyroninophilic and Feulgen negative. By immunofluorescence these inclusions were shown to be depots of viral antigen; specific fluorescence was confined to the cytoplasm throughout the replication of the virus.

Animals↗
PubMed1975

[Cytochemical studies in megakaryocytes in hematologic diseases].

Bone marrow smears of 48 patients consisting of 12 normal cases, 36 patients with different haematological diseases-among them 9 cases of idiopathic thrombopenia, 4 cases of polycythaemia, and 9 cases of Hodgkin's disease - were examined cytochemically. Acid phosphatase, unspecific esterases, naphthol-AS-D-chloroacetate esterase, peroxydase, and leucin-aminopeptidase were represented; in addition the PAS reaction, fastgreen staining at pH 1.1, methyl-green pyronin staining and the lipid representation with Sudan black B were carried out. Besides those responses known from literature the different behaviour of acid megacaryocyte phosphatase in different haematological diseases must be particularly emphasized from all reactions.

Acid Phosphatase↗
PubMed1979

Quantitative histological changes produced in the tracheal mucosa of young chickens by the inhalation of sulfur dioxide in low concentrations.

Chickens were exposed to SO2 in relatively low concentrations (3.4 to 18.5 parts per million (ppm)) for 1 to 14 days. A portion of their tracheas was embedded in water-soluble methacrylate, cut at 2 micrometer and stained with hematoxylin and eosin, Wright's stain, methyl green-pyronin, Alcian blue - periodic and Schiff, and for acid phosphatase. An increase was found in (a) the mucosa to wall ratio; (b) the number of mucosal cells in mitosis; (c) the number of macrophages, lymphocytes, plasma cells, and neutrophils in the epithelium and lamina propria; and (d) the number of these infiltrating cells which contained acid phosphatase. The number of mucus- and seromucus- secreting cells and vasoamine-containing cells were sometimes increased, but not consistently. The percentage of cells containing sialidase-sensitive sialomucins was elevated, and percentage of cells containing neutral mucins was reduced. These changes were only partly related to the SO2 concentration and the duration of SO2 exposure, in that increasing amounts of SO2 did not always cause increasing changes in the mucin composition. Evidently, the altered mucins sometimes protected against further mucin modification.

Acid Phosphatase↗
PubMed1980

Organ culture of Rhipicephalus appendiculatus with maturation of Theileria parva in tick salivary glands in vitro.

A technique is described for the organ culture of Rhipicephalus appendiculatus ticks. Whole, unfed adult ticks with the dorsal integument removed, known as backless tick explants, were cultured in enriched Leibovitz' L-15 medium in which they remained active for at least 32 days at 28 degrees C and 9 days at 36 degrees C. Development of Theileria parva, as demonstrated by methyl green-pyronin staining, occurred in the salivary glands of infected backless tick explants held for 8 days at 28 degrees C or 3 days at 36 degrees C. Maturation in vitro of T. parva in backless tick explants was compared with that in cultured excised salivary glands. After 3-7 days at 36 degrees C glands from backless tick explants and excised salivary glands showed similar numbers of infected acini per infected tick. However, after 12 days at 28 degrees C backless tick explants showed 20-30 times as many infected acini per infected tick as excised salivary glands, in two experiments. No assessment was made of degree of parasite maturity or infectivity. It was concluded that both organ culture techniques supported development in vitro of the salivary gland stages of T. parva, but the backless tick explant technique was simpler and gave generally better results than culture of excised salivary glands.

Animals↗
PubMed1981

Meta-phosphoric acid-its use in the selective extraction of RNA from fixed tissue sections.

This communication presents a new quick method for selective extraction of RNA from formalin-fixed tissues, such as kidney, intestine, ovary and testis of white rat and liver of rodents, Tatera indica and Millardia meltada and of the frog, Rana tigrina as well as of human wart. Sections of pancreas and kidney fixed in acetic acid-alcohol were also tried. The method is to treat deparaffinised sections in 20% and 15% meta-phosphoric acid at 5 degrees C for 10-20 and 20-30 min, respectively, and then to stain nuclear DNA with 0.5% aqueous solution of methyl green, methylene green, Giemsa, toluidine blue O or 0.125% pyronin G for 2 min, rinsed with water, treated with n-butanol for 2-3 min, cleared in xylene and mounted. It has been found that liver sections of rodents require 24 hours of treatment in 15% cold meta-phosphoric acid for complete removal of RNA, whereas those of the frog require only 20 min at 20% acid. It has been concluded that following treatment of sections in cold meta-phosphoric acid, RNA is extracted selectively leaving DNA in a native state. Therefore, staining of DNA in tissue sections from which RNA has been extracted is due to binding of its negatively charged phosphate groups with the positively charged dye molecules.

Animals↗
PubMed1983

Flow cytometry of reticulocytes applied to clinical hematology.

Reticulocytes in fixed human blood samples were stained for RNA with the fluorescent dye pyronin Y and measured by flow cytometry. The resulting relative frequency distributions of the RNA fluorescence intensities concurred with the different stages in maturation from early reticulocytes to mature red cells. A computer program was written to calculate from these frequency distributions the relative number of reticulocytes, their relative RNA content, and the median of the reticulocyte population (RNA index). This method was applied to 30 healthy blood bank donors (control group), as well as to patients with various hematologic disorders showing abnormal erythropoietic activity. The measured percentage of reticulocytes, RNA content, and RNA index were found to correlate well with the various hematologic disorders. Changes in erythropoiesis could be clearly followed, as was demonstrated by analyzing blood samples from children with aplastic anemia or acute myeloid leukemia, who were treated with allogeneic bone marrow transplantation. Measurements on blood samples from healthy blood bank donors showed that with this method, small changes in the reticulocyte population, such as the appearance of polychromatic erythrocytes in the peripheral blood 5-8 hr after donation, can be detected. The statistical reliability and the information provided on the maturation stage of the entire reticulocyte population make flow cytometry of peripheral blood reticulocytes a more informative method for the study of hematologic abnormalities than conventional methods for reticulocyte counting and classification.

Anemia, Aplastic↗
PubMed1983

[Taxonomy and ecology of brucellosis pathogens isolated from Muridae in the northern foothills of the Caucasus mountains. I. Cultural and biochemical properties of Brucella isolated from Muridae].

The results of the study of 65 Brucella strains isolated from myomorphous rodents in the Northern Caucasus are presented. The study was made with the aim of finding out additional characteristics for the identification of these strains. Using the main tests recommended by the FAO/WHO Subcommittee on the Taxonomy of Brucella, as well as some additional tests, we have revealed that the strains under study are very similar to B. suis. At the same time their capacity for agglutination with anti-melitensis monospecific serum, their high sensitivity to pyronin B, safranine T and gentian violet, their low urease activity and their oxidizing activity in respect to L-alanine, L-asparagine, L-glutamic acid, L-arginine, DL-ornithine, DL-citrullin and L-livin make it possible to consider them the fifth independent biotype of B. suis.

Animals↗
PubMed1976

[Modification of chromatids before fixation: production of G bands by various stains].

Acridine orange, coriphosphine, toluidin blue, or pyronin, when added to the hypotonic solution, allow G-banding when the fixed slides are stained in the usual way. Apart from this simple practical application, these modifications of chromatid structure by a treatment applied before fixation shed some light on the molecular mechanisms responsible for chromosome banding.

Chromatids↗