[New localizations in Corsica of Phlebotomus sergenti Parrot, 1917].
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Four tests of an inactivated emulsified vaccine against a herpesvirus infection (Pacheco's disease) in bodgerigars demonstrated that an effective vaccine could be produced with concentrated virus. Virus was propagated in chicken kidney cells, and cells and fluid were harvested 3 to 5 days after inoculation and sonicated. The virus was concentrated 100 x by centrifugation to produce a multiple emulsion vaccine that was protective against IM challenge exposure with live virus. The serologic response after vaccination was negligible, and many survivors to virus challenge exposure did not show a positive titer.
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