Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Microscopy, Phase-Contrast”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 379 records · Page 21Linked to original sources
PubMed1979

[Advantage of phase contrast for the identification of the cellular constituents of urinary sediment].

Phase contrast microscopic observation of the urinary sediment has to be performed on freshly collected urine. This technique, which permits an excellent definition of the cells and the maintenance of intra cellular motility, gives therefore a great accuracy in differential diagnosis, especially for little round cells. Among these, polynuclear granulocytes are easily recognized, because of their lobulated nuclei and of their numerous intra cytoplasmic motile granulations. Lymphocytes and lymphoblasts have round, regular, homogeneous nuclei. Renal tubular cells exhibit eccentric nuclei, with smudged chromatine, and round, refringent cytoplasmic vacuoles. Rapid and easy, this technic should be used for routine microscopic study or urinary sediments.

Adult↗
PubMed1975

Morphologic and functional characteristics of bone marrow macrophages from imferon-treated mice.

The iron storage macrophage has been isolated from the marrow of Imferon-treated mice and studied in vitro by morphologic, histochemical, and functional tests and isotope labeling techniques. These macrophages on stained preparations are large, many times binucleate cells (up to 150 mu), and show Prussian blue reactivity. In Epon-embedded, stained thick sections they contain elongated narrow basophilic inclusions. These macrophages are actively phagocytic and pinocytic; histochemical studies show that these cells are rich in acid phosphatase, nonspecific esterase, and PAS diastase-resistant activity. Iron storage macrophages do not incorporate the 3H-thymidine. The electron microscopic appearance of this macrophage shows that the cell has ferritin free in the cytoplasm and several types of cytoplasmic granules: those with large quantities of electron-dense ferritin and/or hemosiderin (type A), elongated granules (type B) with moderately electron dense homogeneous matrix and some ferritin at the periphery, and granules with heterogeneous content (type C). The above findings demonstrate that the iron storage cell is a mature macrophage which contains hydrolases, ferritin, and a unique population of cytoplasmic granules which are lysosomal in nature. There is some evidence to suggest that the unusual lysosome (type B granule) occurs after macrophages have ingested erythrocytes.

Acid Phosphatase↗
PubMed1975

Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes.

A group of alloantibodies are found in pregnancy sera which react with antigens present on B lymphocytes and monocytes but are not detectable on the vast majority of unstimulated T cells. This specificity distinguishes them from HL-A antibodies which react with both cell types. They were readily recognized through indirect fluorescent antibody analysis by employing the combination of B-cell lymphoid lines and normal peripheral blood T cells. Different sera gave a variety of patterns of reactivity with a panel of 11 lymphoid lines. Similar differential patterns were also observed with normal B cells from different individuals particularly after concentrating the B cells. The antibodies were also cytotoxic to B cells and this procedure gave parallel results to the fluorescence method. The pattern of reactions obtained indicated a very heterogeneous system similar to that for HL-A. Special study of certain of the sera provided evidence that the lymphocyte-defined determinants of the mixed lymphocyte reaction system were involved. For convenience the term HL-B has been employed for these antigens.

Antibody Specificity↗
PubMed1975

[Demonstration of immunoglobulin determinants on lymphocyte surfaces by membrane immunofluorescence].

Isolated lymphocytes from peripheral blood of 22 healthy donors were examined with the help of the indirect membrane immunofluorescence (MIF) for immunoglobulin determinants on the surface of the cells. The quantity of MIF+-cells for IgM was on the average 8.7% (3-18) and for IgG 15.5%(8-18). Five different anti-IgM-sera were characterised with regard to their use in the MIF (precipitation titre, entire protein content, content of anti-IgM-antibodies). Differences in the quantity of the MIF+-lymphocytes were above all based on differing antibody concentrations of the sera. It was referred to further possible causes for these differences. The influence on the results of the examinations by protein addition and trypsination.

Adult↗
PubMed1975

[Cytotoxic effects of a leukocidin from Staphylococcus aureus (author's transl)].

In an attempt to improve the assay of leukocidin from Staphylococcus aureus a combined vital fluorochrome staining procedure with acridine organe and ethidium bromide was developed. It proved to be more suitable for the demonstration of leukocidic effects on leukocytes than phasecontrastmicroscopy. Damaged leukocytes fluoresced distinctly red and undamaged leukocytes green. With the fluorochrome-method leukocidic changes became also evident in some lymphocytes. These were not clearly demonstrated by phasecontrastmicroscopy.

Acridines↗
PubMed1977

[Morphological diagnosis of Pneumocystis carinii].

Pneumocystis carinii pneumonia can only be diagnosed once the microorganism is demonstrated. The authors review and illustrate various staining technics, and discuss for each of them specific advantages. All samples must by systematically examined both by phase contrast microscopy and by light microscopy after Gomori-Grocott and Gram-Weigert stains. This morphological study is completed by ultrastructural photographs.

Humans↗
PubMed1976

Cell separation using fluorescence emission anisotropy.

The emission anisotropy of selected fluorescent probes which interact with cells and their membranes is a sensitive parameter for studying the structural changes associated with different functional states. Such measurements can now be made on individual living cells at rates of up to 103 per second and the cells separated on the basis of the anisotropy function alone or combined with other physical signals using a multiparameter automated computer-controlled cell separator (MACCS). Thus, selection can be on the basis of simple or complex algorithms reflecting the size, macromolecular content, and rotational mobility of cellular components or liganded reporter molecules. Cells isolated in this manner are sterile, viable, and can be used for outgrowth or biochemical studies related to dynamic changes occurring during differentiation or malignant transformation.

Animals↗
PubMed1977

[Fluorescence microscopy demonstration of mitochondria in tissue culture cells using berberine].

The possibility of fluorescence microscopical examination of mitochondria in living animal cells using fluorochrome berberine sulphate is shown. At concentrations of 30--50 g per ml the chemical is accumulated selectively in mitochondria of living cells. The specificity of berberine sulphate accumulation in mitochondria was shown by comparative phase contrast and fluorescence microscopy. The advantages of the method is its high sensitivity and simplicity, especially when mitochondria can not be examined by the phase contrast microscopy.

Animals↗