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At least 379 records · Page 21Linked to original sources
PubMed1996

Laboratory gloves as a source of trace element contamination.

Contamination in a trace element laboratory can come from a variety of sources, including laboratory gloves. Therefore, vinyl and latex gloves were obtained from as many manufacturers as would supply gloves. These gloves were either prepared for acid-washing and subsequent soaking in an acid solution, or immersed in an acid solution for a duration of either 1 min or 1 h. Incubation washes were analyzed for a variety of trace elements by flame atomic absorption spectroscopy (AAS) or inductively coupled mass spectrometry (ICP-MS). Results indicated that only three brands of vinyl gloves were acceptable for use in a trace element laboratory, whereas others had contamination of different elements. Latex gloves contained such high levels of biologically important elements that they were not considered suitable for routine trace element work. Vinyl gloves of choice should be routinely acid-washed before use in a trace element laboratory.

Air Pollution, Indoor↗
PubMed2006

Nitrile glove permeation of benomyl.

The aim of this study was to investigate permeation of the fungicide benomyl at its highest field application concentration (0.70 mg/mL) in Benlate 50 WP aqueous solution (1.4 mg/mL) through two types of unsupported and unlined nitrile gloves--a disposable latex glove (Safeskin) and an industrial chemical-resistant glove (Solvex)--using an American Society for Testing and Materials (ATSM)-type permeation cell with isopropanol collection medium. The permeation cell was contained in a moving-tray water bath at 30.0 degrees C +/- 0.5 degrees C. The collection medium was evaporated and the residue derivatized with an optimized method (2,3,4,5,6-pentafluoro)benzyl bromide to form the disubstituted derivative of carbendazim (CARB), CARB.2PFB. The latter in isooctane was then quantified by gas chromatography- 63Ni-electron capture detection (GC-ECD) by the internal standard method. GC-ECD, GC-mass spectrometry (GC-MS), and reflectance infrared investigations showed that little degradation of benomyl occurred in the challenge solution of aqueous Benlate during an 8-hour exposure period. Benomyl was collected as a mixture of CARB and benomyl as shown by the presence of a diagnostic chromatographic peak identified by GC-MS. The amounts permeated during the same time period were always higher for Safeskin than for Solvex gloves, with the latter being approximately 18 times more protective than the former after 8 hours of continuous exposure. Although the Solvex gloves were safe to wear at least for 4 hours and for almost 8 hours, the ASTM breakthrough threshold was used as reference and thus ignored carcinogenic effects. Reflectance infrared investigations detected benomyl and CARB on the glove challenge surface after drying and confirmed that the cleaned glove surfaces after permeation experiments did not differ in infrared reflectance spectra from the corresponding surfaces just before the permeation experiments.

Benomyl↗
PubMed1989

Glove perforation during plastic surgery.

Intraoperative perforation of surgical gloves is common. Nine hundred and forty surgical gloves were tested after 100 consecutive plastic surgical operations, each involving a surgeon, a variable number of assistants and a scrub nurse. In the first 52 operations, single gloves were used and 21.5% of the staff were found to have a perforated glove. In the second 48 operations, double gloves were used by all members of the surgical team and the number with perforations (of both inner and outer gloves) was reduced to 9%. Most perforations occurred on the dorsum of the hand and fingers and on the thumb tip, especially in the non-dominant hand. The risk of acquiring AIDS due to glove perforation is low but the consequences of such an event could be lethal.

Acquired Immunodeficiency Syndrome↗
PubMed1990

A laboratory assessment of the antimicrobial effectiveness of glove washing and re-use in dental practice.

This study has assessed the ability of five hand-washing agents, Hibisol, Hibiscrub, Betadine, 70% isopropyl alcohol and bar soap, to decontaminate the surfaces of Biogel D, Surgikos Microtouch and Ansell Gammex latex medical gloves after repeated inoculation with Staphylococcus aureus. The glove and hand-washing agents were compared in a Latin Square experiment, and a standardized handwashing regime followed. Each of the gloves used in the study was later tested electrically for micropuncture formation. On the basis of microbial recovery, no specific combination of glove and hand washing agent proved superior, the marker organism being isolated on a sporadic basis. In addition, 17% of the gloves showed evidence of micropunctures after five sequential inoculations and washings. These results suggest that, even under strictly controlled conditions, glove surfaces cannot be reliably and consistently cleaned of microbial contamination, nor can they be guaranteed to remain puncture-free. Ideally, therefore, a new pair of gloves should be worn for each patient.

Cross Infection↗
PubMed1994

Reality of glove use and handwashing in a community hospital.

BACKGROUND: Since the advent of universal precautions and body substance isolation, few studies have examined the relationship between glove use and handwashing. METHODS: During a 5-month period in 1991, 477 structured observations were conducted on 19 patients care units at a community teaching hospital during each of three shifts. Patient care contacts were defined as either high level or low level according to potential for blood or body fluid contact. RESULTS: Health care workers were potentially exposed to body fluids (high-level contact) on 152 occasions. Eighty-eight percent of all high-level contacts were limited to the hands; 47% of these contacts occurred during the night shift. Handwashing occurred after 32% of high-level contacts. Health-care workers wore gloves in 57% of high-level contacts. Rates of handwashing and glove use varied markedly among patient care units. Correct handwashing (> or = 9 seconds) occurred after 20% of contacts when health care workers wore gloves but after only 3% of high-level contacts when gloves were not used (p = 0.004). CONCLUSIONS: Despite universal precautions or body substance isolation, educational efforts, and written policies, rates of handwashing and glove use are inadequate in cases of potential blood and body fluid contact. The perceived need for gloves may encourage handwashing.

Blood↗
PubMed1996

Comfort and frictional properties of dental gloves.

OBJECTIVES: A survey of general dental practitioners and dental surgery assistants was carried out to ascertain their preferences and opinions on powder-free hydrogel-coated gloves compared with starch-powdered gloves. The aim was to relate the survey findings to laboratory measurements of the frictional characteristics of glove inner surfaces and their water absorptive capability. METHODS: The survey was carried out using a questionnaire given to local dental practitioners. Glove friction and water absorption measurements were made using specially designed equipment. RESULTS: The survey showed that a selected group of dentist and dental surgery assistants preferred hydrogel-coated gloves, particularly for damp donning, durability and long-term wear comfort. Laboratory measurements showed that the hydrogel coating gave a low friction coefficient against damp skin. The coating was durable, and absorbed water more readily than other treatments. CONCLUSION: A survey of dental practitioners and dental surgery assistants and laboratory measurements indicates that hydrogel-coated gloves have superior properties, and are preferred to other non-sterile glove types.

Absorption↗
PubMed2006

Comparison of bacterial counts in glove juice of surgeons wearing smooth band rings versus those without rings.

BACKGROUND: Removal of rings is recommended before surgeons aseptically prepare for surgical procedures. OBJECTIVES: This study was undertaken to determine whether there is a difference between bacterial counts under surgical gloves of ring wearers compared with nonring wearers after proper aseptic hand preparation and 3 hours of wear. METHODS: Twenty volunteer veterinary medical students were divided into 2 groups: One group wore a smooth ring band (without projections or mounted stones) on their ring finger, and the other group did not wear a ring. A modified glove juice method was used to obtain bacterial counts (colony-forming units/mL) inside surgically gloved hands prior to a proper aseptic hand preparation and 3 hours after hand preparation and wear. Each of the pre- and postsurgical glove juice samples were inoculated onto Letheen agar plates, which were incubated aerobically under atmospheric conditions for 48 hours at 35 degrees C. Gloves were tested for perforations using a water pressure test. RESULTS: No differences, or significant change, in bacterial counts were found before or after surgery between all ring hands and nonring hands or between ring and nonring hands for ringed participants. No differences in bacterial counts were found within perforated versus nonperforated gloves. CONCLUSION: There is no compelling evidence to suggest that surgeons wearing rings possess higher bacterial counts under their gloves during surgery.

Antisepsis↗
PubMed2006

Effect of dental tool surface texture and material on static friction with a wet gloved fingertip.

Hand injuries are an important cause of pain and disability among dentists and dental hygienists and may be due to the high pinch forces involved in periodontal work. The pinch forces required to perform scaling may be reduced by increasing the friction between the tool and fingers. The purpose of this study was to determine whether modifying the tool material, surface texture, or glove type altered the coefficient of static friction for a wet gloved finger. Seven tools with varying surface topography were machined from 13 mm diameter stainless steel and Delrin and mounted to a 6-component force plate. The textures tested were a fine, medium and coarse diamond knurled pattern and a medium and fine annular pattern (concentric rings). Thirteen subjects pulled their gloved, wet thumb pad along the long axis of the tool while maintaining a normal force of 40 N. Latex and nitrile gloves were tested. The coefficient of static friction was calculated from the shear force history. The mean coefficients of static friction ranged from 0.20 to 0.65. The coefficient of static friction was higher for a smooth tool of Delrin than one of stainless steel. Differences in the coefficient of static friction were observed between the coarse and medium knurled patterns and the fine knurled and annular patterns. Coefficients of static friction were higher for the nitrile glove than the latex glove for tools with texture. These findings may be applied to the design of hand tools that require fine motor control with a wet, gloved hand.

Biomechanical Phenomena↗
PubMed1996

Natural rubber latex skin testing reagents: safety and diagnostic accuracy of nonammoniated latex, ammoniated latex, and latex rubber glove extracts.

BACKGROUND: Nonammoniated latex, ammoniated latex, and rubber glove extracts are the only sources of natural rubber (Hevea brasiliensis) latex that have potential for use as skin testing reagents in the diagnosis of latex allergy. Their diagnostic sensitivity and specificity as skin test reagents are unknown. OBJECTIVE: We conducted a phase 1/2 clinical study to examine the safety and diagnostic accuracy (sensitivity and specificity) of nonammoniated latex, ammoniated latex, and rubber glove extracts as skin test extracts to identify the most efficacious source material for future skin test reagent development. METHODS: Twenty-four adults not allergic to latex, 19 adults with hand dermatitis or pruritus, and 59 adults with a latex allergy were identified by clinical history. All provided blood and then received puncture skin tests and intradermal skin tests with nonammoniated latex, ammoniated latex, and rubber glove extracts from Malaysian H. brasiliensis latex by use of sequential titration. A glove provocation test and IgE anti-latex RAST were used to clarify positive history-negative skin test response and negative history-positive skin test response mismatches. RESULTS: All three extracts were biologically safe and sterile. After normalization to 1 mg/ml of total protein, all three extracts produced equivalent diagnostic sensitivity and specificity in puncture skin tests and intradermal skin tests at various extract concentrations. Optimal diagnostic accuracy was safely achieved at 100 micrograms/ml for intradermal skin tests (e.g., nonammoniated latex: puncture skin test sensitivity 96%, specificity 100%; intradermal skin test sensitivity 93%, specificity 96%). The presence of IgE antibody in skin was highly correlated with IgE anti-latex in serum (nonammoniated latex: r = 0.98, p < 0.001; ammoniated latex: r = 0.94, p < 0.001; rubber glove extract: r = 0.96, p < 0.001). All five available subjects with a positive history, negative skin test response, and absence of IgE antibody in serum had a negative glove provocation test response, indicating no clinical evidence of latex allergy. No systemic or large local allergic reactions were observed with puncture skin tests or intradermal skin tests. CONCLUSIONS: Equivalent diagnostic sensitivity and specificity were observed with the nonammoniated latex, ammoniated latex, and rubber glove extract skin test reagents after normalization for total protein; nonammoniated latex may be considered the reagent of choice on the basis of practical quality control and reproducibility considerations.

Adult↗
PubMed1998

Loss of glove integrity during emergency department procedures.

STUDY OBJECTIVE: We sought to assess the loss of integrity of the latex-glove barrier during use in the ED setting. METHODS: We conducted a prospective observational study in an urban ED and Level I trauma center. Procedures performed by ED health care workers (HCWs) were directly observed, timed, and categorized. The gloves used by the HCWs were collected and subjected to the standard US Food and Drug Administration leak test. RESULTS: Ninety-nine (7.9%) of 1,254 pairs of gloves used for observed procedures leaked, compared with 2 of 200 unworn control pairs (1.0%) and 12 of 300 pairs that were worn but not used (4.0%). Leak rates varied by manufacturer and were higher for gloves worn 20 minutes or longer (13.7%, P = .015), used for four or more procedures (50%, P < .01), or used for critical care procedures (23.5%, P < .01). Sixty-six of an additional 325 pairs of gloves collected from unobserved critical care procedures (20.3%) leaked. CONCLUSION: Loss of glove integrity occurs during the performance of ED procedures, subjecting the HCW to possible infectious-fluid exposure. Risk of glove perforation increases with duration of wear, number of procedures performed, and the performance of critical care procedures.

Critical Care↗
PubMed1991

Glove use by health care workers: results of a tristate investigation.

The Center for Devices and Radiological Health, in collaboration with the state health departments of Iowa, Maryland, and Massachusetts, conducted a multi-state, multi-institutional investigation of glove use by health care workers (HCWs). Twenty-two hospitals and four ambulatory care centers were included in the investigation. All 26 health care facilities were found to have adopted universal precautions policies for glove use by HCWs, per Centers for Disease Control guidelines. Four hundred five observations were made of HCWs performing procedures that may involve contact with patient body fluids, particularly blood. The prevalence of glove use during selected procedures was as follows: arterial blood gas procedures, 92.3%; intravenous line initiation/maintenance, 77.6%; and phlebotomy, 70.6%. Glove use during phlebotomy (p less than 0.001) and intravenous line procedures (p less than 0.05) was significantly lower in the state with a prevalence of the acquired immunodeficiency syndrome (AIDS) below the national average than in the states with a higher AIDS prevalence. The investigation suggests that health care facilities have responded to the Centers for Disease Control and Occupational Safety and Health Administration campaign to adopt universal precaution policies for glove use by HCWs. Actual glove use by HCWs appears to be substantial but not universal. Glove use by HCWs is significantly related to statewide AIDS prevalence.

Acquired Immunodeficiency Syndrome↗
PubMed1996

Glutaraldehyde permeation: choosing the proper glove.

Six different gloves were tested with five different aqueous glutaraldehyde formulations to determine each glove's resistance to permeation. When tested against 2% or 3.4% glutaraldehyde solutions, nitrile rubber, butyl rubber, a synthetic surgical glove, and polyethylene were each impermeable for at least 4 hours. The two latex gloves tested showed glutaraldehyde breakthrough at 45 minutes. When the latex gloves were doubled, the time to first breakthrough increased to 3 to 4 hours. With 50% glutaraldehyde, only butyl rubber and nitrile rubber were impermeable for extended periods. The surgical synthetic glove had breakthrough at 1 hour, whereas polyethylene and the two latex gloves had breakthrough in less than 1 hour.

Evaluation Studies as Topic↗
PubMed1999

Glove resistance to permeation by a 7.5% hydrogen peroxide sterilizing and disinfecting solution.

BACKGROUND: This study evaluated 6 types of glove materials for resistance to permeation by a 7.5% hydrogen peroxide liquid chemical germicide. METHODS: Based on American Society for Testing and Materials Method F739-96, a small piece of glove material was placed in the center of a dual chamber test cell. The challenge and collection chambers of the test cell were filled with the hydrogen peroxide test chemical and the collecting medium (deionized water), respectively. Chemical permeation was determined by measuring the concentration of hydrogen peroxide in the collection medium at various time intervals. RESULTS: Both the polyvinylchloride and disposable latex examination gloves at 4.5 mil thickness provided less than 30 minutes of protection from hydrogen peroxide. The natural rubber latex glove at 16.5 mil lasted for 8 hours without any detectable penetration. Neoprene (15 mil) and nitrile butyl rubber (18 mil) both provided excellent protection throughout the 8-hour test period. CONCLUSION: Disposable latex examination gloves should be used for short-term exposure to hydrogen peroxide solutions. Thicker rubber latex gloves provided adequate protection and necessary dexterity for conducting intricate tasks. The level of protection depends largely on the thickness and quality of the glove material.

Anti-Infective Agents, Local↗
PubMed2002

Allergenicity of natural rubber latex gloves.

The concentrations of proteins, allergens and rubber chemicals are essential parameters of the allergenicity of NRL gloves. To date, a standardized method has been given only for analysis of the protein concentration (DIN EN 455-3), and not for the concentrations of allergens and rubber chemicals. In the present study, we investigated 11 brands of surgical gloves currently available on the German market. Additionally 1 glove, not subjected to final leaching procedures, was added for comparison purposes. Protein concentrations were analysed by different methods in different laboratories. Allergen concentrations were assayed by prick tests in NRL-allergic volunteers and by RAST inhibition methods. Rubber chemicals were analysed by HPTLC and GC. The protein concentrations analysed by the Lowry method in the 2 laboratories gave concordant results, but the correlations between protein and allergen concentrations were low. The protein concentration analysed by HPLC correlated with the allergen concentration, and gave better information on the allergenicity of the gloves. The development of standardized methods for allergen analysis in the assessment routine is necessary, due to significant discrepancy between protein and allergen levels of some gloves. Thiurams were not found in any of the gloves, though carbamates were present in all gloves tested. Our data indicate that washing procedures have little or no effect on the concentration of rubber chemicals.

Adult↗
PubMed2002

Perforation rates for nonsterile examination gloves in routine dermatologic procedures.

BACKGROUND: Low cost, nonsterile examination gloves are used routinely to perform various dermatologic procedures. OBJECTIVE: To evaluate the perforation rate of nonsterile examination gloves in routine dermatologic procedures. METHODS: Three hundred fifty nonsterile latex examination gloves used to perform shave biopsies were evaluated for perforations using an air inflation/water submersion method. Ninety gloves, which were intentionally perforated with a 30-gauge needle, were used as controls to assess our evaluation method. RESULTS: Eight of the 350 gloves were found to have a perforation, which corresponds to a 2.3% perforation rate. Seven of the eight perforations were found in the web space between the second and third finger sleeves, with one being an obvious manufacturing error. All 90 perforations of the control group were correctly identified. CONCLUSION: There appears to be a very low risk of glove perforation when nonsterile examination gloves are used in routine dermatologic procedures.

Biopsy↗
PubMed1997

Effect of work glove and type of muscle action on grip fatigue.

Fatigue plays a major role in limiting work performance. The objectives of this study were: (1) to determine the effect of wearing a work glove on handgrip fatigue; (2) to compare the effect of a sustained grip contraction of concentric versus eccentric nature, named in this study isometric and eccentric; and (3) to determine if there is a relationship between physiological muscle performance and subjective perceptional fatigue during isometric and eccentric gripping. The study had 2 x 2 repeated measures design. The two factors were: (1) glove condition (glove, no glove); and (2) type of contraction (eccentric and isometric). The measurements taken were: (1) time to limit of endurance (Tlim); (2) rate of perceived effort (RPE); (3) mean power frequency (MPF) derived from the electromyogram (EMG); and (4) the fatigue objective-subjective relationship (FOSR, which is the correlation coefficient between RPE and MPF). Twenty-one normal subjects maintained a handgrip of 60% of their maximal effort until exhaustion for each of the four conditions. During each trial EMG values were recorded every 5 s and RPE values were recorded every 10 s. The RPE was recorded as a subjective value selected from a 10-point Borg scale. The results showed that Tlim was greater for the no glove condition (p < 0.0005) and for the eccentric muscle action (p < 0.05). The FOSR was greatest for the glove condition (p < 0.03) and for the isometric muscle action (p < 0.013). The MPF decline showed no significant difference for any condition. These data indicate that glove condition and type of handgrip contraction have an effect on physiological fatigue and subjective perception of fatigue.

Adult↗
PubMed2002

In-use testing and interpretation of chemical-resistant glove performance.

Issuing gloves to workers is the most common approach to protecting against skin contact with hazardous chemicals. Typically, glove materials are selected and duration of wear is estimated based on comparisons of laboratory test data. Those who select the glove materials often fail to verify their selections by testing the glove during actual use. This failure poses a common but potentially serious hazard to workers. Although methods are available for assessing permeation rates during actual use, such testing is unlikely without acceptable exposure guidance criteria for decision making. This document reviews methods for testing glove performance during actual use and suggests an approach for estimating acceptable exposure guidance criteria for evaluation of chemicals that are systemically absorbed. It is the authors' opinion that as of now an approach to estimating exposure criteria for chemical irritants and sensitizers may not be feasible. With available data resources, acceptable glove exposure criteria could be generated for use in assessing the risk of using specific gloves for handling many compounds in occupational settings.

Evaluation Studies as Topic↗
PubMed2003

Resistance of medical gloves to permeation by methyl methacrylate (MMA), ethylene glycol dimethacrylate (EGDMA), and 1,4-butanediol dimethacrylate (1,4-BDMA).

Gloves afford hand protection by minimizing skin contact. The effectiveness of medical gloves to protect against permeation of the monomers, methyl methacrylate (MMA), ethylene glycol dimethacrylate (EGDMA), and 1,4-butanediol dimethacrylate (1,4-BDMA), was assessed focusing on permeation rates and degradation of glove materials caused by monomer contact. Fifteen different brands of gloves were tested using a European Standard procedure. Surface images of glove materials before and after exposure to the monomer mixture were obtained using a scanning electron microscope. The standard is not applicable as the only method for estimating the safety of gloves, but it is useful as guideline together with the cumulative permeation of acrylic monomers. Monomer contact on the outside resulted in substantial swelling of most glove materials, and structure changes of the inside surface.

Gloves, Surgical↗