[The bis- and mono-digitoxosides of digitoxigenin and digoxigenin: metabolites of digitoxin].
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Digitalis↗
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Results for “Digoxigenin”
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The action of digoxin and digoxigenin-monodigitoxoside on the sodium pump and on the contractility in isolated guinea-pig atria.
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Animals↗
Preparation of digoxigenin-labeled probes by the polymerase chain reaction.
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Base Sequence↗
Suppression by sodium, potassium or nucleotides of binding between cardiac steroid (digoxigenin) and sodium- and potassium-dependent adenosine triphosphatase formed in the presence of magnesium and phosphate.
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Adenosine Triphosphate↗
Iodinated digoxigenin derivatives.
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Digoxigenin↗
Variation of trace binding in digoxin radioimmunoassay by use of 125I-labeled 3-O-succinyl-digoxigenin: relation of thyroxine concentration to binding.
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Digoxigenin↗
Background reduction in northern analysis by preabsorption of digoxigenin-labeled riboprobes.
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Absorption↗
The DIG (digoxigenin) shift assay.
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Digoxigenin↗
UV cross-linking of RNA to nylon membrane is suitable for northern blot hybridization using a digoxigenin-labeled DNA probe.
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Animals↗
[Detection of polynucleotide fragments using hybridization with probes labelled with digoxigenin].
A new nonradioactive system for labeling molecular probes, using digoxygenine, was studied. Detection of ADN-ADN type hybrids formed on a nylon membrane was realised photochemically or by ELISA, dot-blot or Southern-blot. The high sensitivity and specificity of this new system allow to use it for detection of amounts of ADN as small as 5 ng.
DNA↗
A single-tube method for the preparation of digoxigenin-labeled probe from RNA by PCR.
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Base Sequence↗
Inhibition of RNA polymerases by digoxigenin-labeled UTP.
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DNA-Directed RNA Polymerases↗
Efficient removal of hybridization signal from RNA dot blots probed with digoxigenin-labeled cRNA.
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Digoxigenin↗
Multiple hybridization analysis. A simultaneous use of two or three non-radioactive labels for DNA-probes: biotin, digoxigenin and trans-diamminedichlorplatinum.
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Alkaline Phosphatase↗
Blot analysis of glycoconjugates using digoxigenin-labeled lectins: an optimized procedure.
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Digoxigenin↗
Stripping of digoxigenin-labeled probes from nylon membranes.
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Digoxigenin↗
[Detection of gene expression using digoxigenin-labeled probes].
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Digoxigenin↗
Biotin- or digoxigenin-conjugated nucleotides bind to matrix vesicles in atherosclerotic plaques.
The present study analyzes the staining pattern of DNA in situ end-labeling techniques of human and rabbit atherosclerotic plaques. Both the terminal deoxynucleotidyl transferase end-labeling and the in situ nick translation technique detected, besides apoptotic nuclei, numerous round vesicles with diameters from 0.5 to 5 microns within the atherosclerotic plaques. These vesicles did not contain DNA but contained calcium. A pretreatment with EDTA or citric acid abolished the labeling of the vesicles but did not influence the detection of apoptotic nuclei. Ultrastructurally, the vesicles were of variable diameter and density, and their aspect was compatible with matrix vesicles, which are well known in epiphyses during bone formation. The larger vesicles contained cell organelles, and the small vesicles were very dense. X-ray microanalysis demonstrated high calcium and phosphorus levels within the most dense vesicles. Different stages of the process were present in the plaques. In this way we could demonstrate that cytoplasmic fragmentation of smooth muscle cells and subsequent formation of matrix vesicles are a frequent finding in atherosclerotic plaques. The association of apoptotic cell death and formation of matrix vesicles could be an interesting pathway in explaining calcification of atherosclerotic plaques. Both the terminal deoxynucleotidyl transferase end-labeling and the in situ nick translation technique detected simultaneously apoptotic nuclei and matrix vesicles if calcium is not removed from the sections.
Animals↗