Components of fraction 3 and pepsin-digested fraction 3 from papain-digested rabbit gamma-globulin.
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Activity of disaccharidases in various portions of the small intestine markedly increased in 6-month-old offspring of pregnant females feeding a protein-deficient diet. Activities of amino- and dipeptidases decreased in the kidneys, while alkaline phosphatase activity remained practically unchanged. Probably, information about protein restriction in nutrition of pregnant females is stored in the biochemical memory of adult offspring and promotes the development of metabolic disturbances.
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Aqueous solutions of DNA were gamma-irradiated in the presence and absence of oxygen and enzymatically hydrolysed by the combined action of pancreatic deoxyribonuclease (DNase I), snake-venom phosphodiesterase (PDE I), spleen phosphodiesterase (PDE II) and alkaline phosphatase. In contrast to unirradiated DNA, which is fully hydrolysed to nucleosides by these enzymes, gamma-irradiated DNA yields a series of oligonucleotides. Their isolation might enalbe the future identification of the chemical nature of DNA lesions.
Secretion into the Gastro-Intestinal Tract of Amino Acids Labelled with Radioactive Isotopes. (4) 14C-Labelling and 14C-Leucine in the Gastro-Intestinal Tract after Subcutaneous Injection of 14C-Leucine to Rats with Tied-off Pancreatic Gland.
From two fattening pigs (average live weight 63 kg) with re-entrance cannulae both in the duodenum and the ileocecal region, the intake, the excretion and the flow rates of nitrogen, 15N, lysine and 15N-lysine are measured after the oral application of 15N-lysine as supplement of a wheat-wheat gluten diet. A scheme of the lysine metabolism of pigs is drawn on the basis of the measured values. The absorption of synthetic and native lysine as well as the endogenous and exogenous lysine contents of the duodenal and ileum chyme are estimated.
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