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PubMed2000

Seasonal variability in the biophysical properties of stratum corneum from different anatomical sites.

BACKGROUND/PURPOSE: A 10-month-long study on a panel of 24 young female subjects was carried out to determine whether various biophysical aspects of the stratum corneum (SC) varied with season. METHODS: Three different anatomical sites (calf, inner forearm and crow's foot wrinkle area of the face) were assessed in February, April, July and December of the same year. The assessments made were skin surface hydration using an electrical capacitance technique, transepidermal water loss by evaporimetry, number of corneocytes released using a turbine stimulation method, and skin surface topography using optical profilometry. RESULTS: The results showed significant anatomical differences: with the crow's foot site > forearm > calf, for skin surface hydration and corneocyte numbers; and the crow's foot site > forearm and calf, with no difference between the latter two sites, for TEWL measurements. With these techniques, seasonal differences were observed mainly in the calf, to a lesser extent in the forearm, but not in the crow's foot area. These mainly involved increases in these three parameters in July, as opposed to the other time-points. Parameters of skin surface topography however, showed no consistent seasonal pattern, but markedly higher values were observed for the forearm in comparison to the calf and crow's foot sites, which were similar. From meteorological data obtained, the average daily maximum temperature and hours of sunshine increased to peak values in July, as did the absolute humidity derived from relative humidity data. CONCLUSION: We conclude that the results are most likely to represent changes in the SC due to climatic factors, with the calf and forearm sites being most affected in comparison to the face (crow's foot wrinkle area), which seems unaffected. The reasons for this latter site remaining unchanged may be due to its greater UV exposure, sebum content, and that the use of facial cosmetics was allowed.

Journal Article↗
PubMed1975

A method for the objective study of tool-using behavior.

Key pecking for food was shaped in four crows within a conventional operant-conditioning test chamber. When pecking stabilized, a metal screen with openings 2.5 cm high by 1.0 cm wide, was placed over the response key, so that the crow could still see but could no longer peck the key. At the same time, several dozen wooden matchsticks, which could be used to operate the key, were placed in the test chamber. The crows made no use of these during 50 to 75 hr of exposure to this condition. Subsequently, the behavior of two crows was shaped so that they approached the matchsticks, picked one up in their beaks, approached the response key with the matchstick in their beak, and finally operated the response key by poking the matchstick through the screen. This shaping procedure was ineffective with the two other crows. However, these birds were successfully trained through positional fading of the tool. This involved suspending a metal rod from the ceiling so that it hung directly in front of the response key, and the crow had only to peck it to operate the key. Then, the rod was gradually lowered by lengthening its tether until it eventually rested on the floor of the test chamber. The principal advantage of this methodology is the automatic recording of the terminal (tool-using) behavior under study.

Journal Article↗
PubMed2005

Total hip replacement with the CLS expansion shell and a structural femoral head autograft for patients with congenital hip disease.

BACKGROUND: Reconstruction of a dysplastic acetabulum in a patient with osteoarthritis of the hip may be accomplished with a variety of surgical techniques. The aim of our study was to assess the outcomes of total hip replacement with the uncemented CLS expansion shell and a structural femoral head autograft to augment the deficient acetabulum in patients with osteoarthritis secondary to congenital hip disease. METHODS: Between 1990 and 1994, we used a CLS expansion shell with a structural femoral head autograft in forty-three consecutive patients (forty-three hips) with osteoarthritis secondary to congenital hip disease. The ratio of male to female patients was 5:38, and the mean age of the patients was forty-eight years. According to the preoperative radiographic assessment, the dysplasia was categorized as Crowe type I in six patients, Crowe type II in thirty-one patients, and Crowe type III and type IV in three patients each. No patient was lost to follow-up. The mean duration of follow-up was 120 months. Plain radiographs were made immediately after surgery and at the latest follow-up evaluation. Clinical outcomes were determined with use of the Harris hip score and the Merle d'Aubigné and Postel score, and a radiographic analysis was performed. RESULTS: Postoperatively, the mean Harris hip score had improved 58 points for patients with Crowe type-I and II dysplasia, 47 points for patients with Crowe type-III dysplasia, and 46 points for patients with Crowe type-IV dysplasia (p < 0.05 for all). At the latest follow-up examination, the mean Harris hip score for all patients was 92.6 points. The mean Merle d'Aubigne and Postel score was 8.3 points preoperatively and 15.8 points at the time of the latest follow-up. The mean coverage of the shell by the graft immediately after surgery was 32.2%. Osteointegration of the CLS expansion shell was evident radiographically in all forty-three hips at the latest follow-up evaluation. There were no failures of the bone grafts. Clinical survival of the CLS expansion shell with a structural femoral head autograft was 100% at a mean of ten years after surgery. The rate of survival of the shell, with radiographic signs of loosening as the end point, was 88.2% at ten years. CONCLUSIONS: The CLS uncemented expansion shell, when used with a structural femoral head autograft, provides a reliable reconstruction, augments deficient acetabular bone stock, and allows placement of the socket at or close to the anatomic center of hip rotation in patients undergoing total hip arthroplasty to treat the sequelae of congenital hip disease.

Acetabulum↗
PubMed2002

Evidence for species differences in the pattern of androgen receptor distribution in relation to species differences in an androgen-dependent behavior.

Chickens (Gallus gallus domesticus) and Japanese quail (Coturnix japonica), two closely related gallinaceous bird species, exhibit a form of vocalization-crowing-which differs between the species in two components: its temporal acoustic pattern and its accompanying postural motor pattern. Previous work utilizing the quail-chick chimera technique demonstrated that the species-specific characteristics of the two crow components are determined by distinct brain structures: the midbrain confers the acoustic pattern, and the caudal hindbrain confers the postural pattern. Crowing is induced by androgens, acting directly on androgen receptors. As a strategy for identifying candidate neurons in the midbrain and caudal hindbrain that could be involved in crow production, we performed immunocytochemistry for androgen receptors in these brain regions in both species. We also investigated midbrain-to-hindbrain vocal-motor projections. In the midbrain, both species showed prominent androgen receptor immunoreactivity in the nucleus intercollicularis, as had been reported in previous studies. In the caudal hindbrain, we discovered characteristic species differences in the pattern of androgen receptor distribution. Chickens, but not quail, showed strong immunoreactivity in the tracheosyringeal division of the hypoglossal nucleus, whereas quail, but not chickens, possessed strong immunoreactivity in a region of the ventrolateral medulla. Some of these differences in hindbrain androgen receptor distribution may be related to the species differences in the postural component of crowing behavior. The results of the present study imply that the spatial distribution of receptor proteins can vary even between closely related species. Such variation in receptor distribution could underlie the evolution of species differences in behavior.

Animals↗
PubMed2004

Extended upper blepharoplasty for lateral hooding of the upper eyelid using a scalpel-shaped excision: a 13-year experience.

Excess skin of the upper lids is often accompanied by lateral overlap of skin with crow's feet because of the absence of fixation to the tarsal plate, giving the eye a sad, heavy look that often disturbs the lateral visual field. The accepted crescent-shaped blepharoplasty is somewhat convex, which is widest at the center of the lid with or without a lateral extension. However, in patients who have normal brow position or minimal eyebrow ptosis and whose main concern is the excess upper eyelid skin and lateral hooding, such a crescent excision may not suffice. A scalpel-shaped excision that is widest laterally and that tapers to a point medially will extirpate the maximal skin where it is most needed and overcome the skin excess in the lateral aspect of the upper lid. Between 1990 and 2002, 301 white patients (275 women and 26 men) between the ages of 33 and 79 years were operated on using the extended scalpel-shaped upper blepharoplasty technique. The follow-up period was more than 1 year. The lower margin of the incision is along the supratarsal crease, about 10 mm above the ciliary line. It begins medially about 1 cm above and lateral to the medial canthus. Above the lateral canthus, the skin marking is gently curved upward and outward, often within a natural skin crease or crow's feet to reach a little below and slightly beyond the lateral extremity of the eyebrow. The upper border of the incision joins the two extremities of the skin outline in a gentle convex curve. The general outline of the incision takes on the shape of a number 20 scalpel blade in which the maximal width is located laterally. Following excision of the excess skin and removal of protuberant fat pads if needed, suturing is executed from lateral to medial. The final suture line is in the form of an oblique flattened lazy S. Following the removal of the stitches on the fifth postoperative day, no wound dehiscence was noticed at the lateral scar zone. In the older individuals, due to the lax skin, the scar becomes scarcely noticeable with time and often falls within a pre-existent crow's feet crease. Elimination of some of the crow's feet was also demonstrated. In patients with visual field impairment, significant functional and visual improvement was achieved. Most patients mentioned a pleasing postoperative open "Oriental" look of the eyes. The extended scalpel-shaped upper blepharoplasty adequately deals with the hooding of the skin laterally. This technique overcomes the excess of skin in both vertical and horizontal directions, since in suturing the lateral part of the skin defect in an oblique plane, slack skin is taken up transversely, and the technique provides some indirect upward support to the lateral eyebrow. In the absence of crow's feet in the younger person, this technique is not recommended because the lateral part of this suture line is visible, especially if the scar widens.

Adult↗
PubMed2001

Optical pulse propagation and holographic storage in a coupled-resonator optical waveguide.

We propose a method of storage and reconstruction of a classical light pulse based on photorefractive holography in a coupled-resonator optical waveguide (CROW). Pulse propagation in a CROW is described in the context of the tight-binding approximation; the use of a CROW results in a large reduction of the group velocity, which is important for spatial compression of the optical pulses. Further, the efficiency of the photorefractive effect is enhanced in a CROW, enabling quasistatic holographic grating formation using much lower intensity optical pulses. We describe in detail the formation of a photorefractive index grating in a CROW via interference with a reference pulse and the subsequent holographic reconstruction of the signal pulse.

Journal Article↗
PubMed2003

Rapid antigen-capture assay to detect West Nile virus in dead corvids.

The utility of the VecTest antigen-capture assay to detect West Nile virus (WNV) in field-collected dead corvids was evaluated in Manitoba and Ontario, Canada, in 2001 and 2002. Swabs were taken from the oropharynx, cloaca, or both of 109 American Crows, 31 Blue Jays, 6 Common Ravens, and 4 Black-billed Magpies from Manitoba, and 255 American Crows and 28 Blue Jays from Ontario. The sensitivity and specificity of the antigen-capture assay were greatest for samples from American Crows; oropharyngeal swabs were more sensitive than cloacal swabs, and interlaboratory variation in the results was minimal. The sensitivity and specificity of the VecTest using oropharyngeal swabs from crows were 83.9% and 93.6%, respectively, for Manitoba samples and 83.3% and 95.8%, respectively, for Ontario birds. The VecTest antigen-capture assay on oropharyngeal secretions from crows is a reliable and rapid diagnostic test that appears suitable for incorporation into a WNV surveillance program.

Animals↗
PubMed2000

[On the phylogenetic relationship of Corvinae birds (Aves, Corvidae) from data of partial sequencing of cytochrome b gene mitochondrial DNA].

To establish phylogenetic relationships within the corvine birds at the interspecific and intergeneric levels, the sequence of the mitochondrial DNA cytochrome b gene was analyzed. The NJ, UPGMA, and MP trees showed similar clustering. Relationships between the jungle crow, on the one hand, and the rook and Australian raven, on the other hand, were closer than between the jungle crow and the hooded and carrion crows. Mitochondrial genome of Australian raven displayed the closest similarity to the ancestral genome of the genus Corvus. Populations inhabiting the eastern part of the carrion crow C. corone orientations area were statistically significantly subdivided into three lineages. These data also confirmed the hypothesis on the location of the carrion crow ancestral lineage in the southeastern part of the area. In general, the transition and transversion substitution levels, their relationships, and distribution over codon positions were similar to that already reported for birds. Synonymous transitions in the third codon position were the prevailing substitution type. Using standard calibration scales, the time of divergence between species and genera within the corvine family was estimated to be 3.1-4 and 3.8-8.8 Myr, respectively. The divergence time between the examined corvine birds and birds of paradise constituted from 8 to 10 Myr.

Cytochrome b Group↗
PubMed2001

[Karyological study of some Corvine birds (Corvidae, aves)].

Karyotypes were studied in the hooded and carrion crows, their naturally occurred hybrids, the jungle crow, the azure-winged magpie (2n = 80 in all aforementioned birds), and the magpie (2n = 82). Corvine birds of Primorskii Krai were karyotyped for the first time. In addition to the similarity in the diploid chromosome sets, corvine birds were shown to have a similar structure of karyotype: in all studied birds, 14 macrochromosomes (Mchs) classified into three groups according to their size were detected. By karyotype structure, birds belonging to the same genus are similar. Some intergeneric differences are due to a change in the position of centromeres of the largest and sex chromosomes. Karyotypes of interspecific hybrids of crows are remarkable for the presence of heteromorphic (t/st) chromosome pair 2 in some individuals, which apparently does not affect their fecundity. Using differential C-banding, the sex chromosome W in female magpies was identified. In addition, heteromorphism was detected in C-bands of homologs of Mch pair 4 in the hooded crow. In the jungle crow, the azure-winged magpie, and the magpie, bright QH-bands and numerous G-bands were detected on Mchs and on some microchromosomes only. Active Ag-NOR-bands were detected on one macrochromosome pair in the magpie. In all, the karyotype structure of corvine birds is comparable to the basic structural scheme of the karyotype in the order Passeriformes, which confirms the concept of conservatism of the avian karyotype.

Animals↗
PubMed2001

"Neon needles" in a haystack: the advantages of passive surveillance for West Nile virus.

Passive surveillance is usually viewed as less efficient for case ascertainment than active surveillance. However, for diseases with nonhuman animal reservoirs, active surveillance can be like looking for a needle in a haystack and may be prohibitively expensive. Fortunately for surveillance of West Nile virus (WNV) in the northeast US, the dead crows have served as "neon needles in a haystack"--indicators of viral activity that call attention to themselves. In 2000, laboratory testing of dead birds, including all species, birds found singly, with signs of trauma, or no compatible pathology, provided the first confirmation of viral activity in most areas. The surveillance factor most closely associated with the number of human cases was the dead crow density. In 2001, dead crow densities will be used as an additional index for monitoring human risk and need for prevention and control activities. If there are few crows in an area, if their case-fatality rate is reduced, or if there is public complacency about reporting dead crow sightings, this passive surveillance indicator may not be helpful in identifying areas likely to have occasional human cases or an outbreak.

Animals↗
PubMed1981

Organochlorine pesticide residues in some Indian wild birds.

Residues of BHC and DDT were estimated by gas-liquid chromatographic analysis of the internal body organs, depot fat, and blood plasma of a few species of Indian wild birds, captured in and around the urban area of Lucknow. Total BHC and gamma-BHC (lindane) levels were high in breast muscle, liver, heart, and lung tissues of pigeon, crow, and vulture, compared with the respective tissues of chicken, cattle egret, and kite. More lindane and total BHC was found in tissues of vulture compared with other species. The major part of BHC isomers in the brain of birds examined was accounted for by alpha-BHC. Total BHC detected in depot fat of crows was 29.7 ppm; lesser amounts were found in vulture, kite, and cattle egret, respectively. Total DDT levels were comparable in the blood plasma of chicken, pigeon, crow, and cattle egret, although residues generally showed the following order in the tissues examined: chicken less than pigeon less than cattle egret less than crow less than kite less than vulture. High levels of DDT were detected in depot fat of crow, kite, and vulture (50.8, 67.0 and 95.3 ppm, respectively). Avian species thus reflect biological magnification of BHC and DDT residues, presumably due to their food habits.

Animals↗
PubMed1993

Management of neuropathic arthropathy with the Charcot Restraint Orthotic Walker.

A recently designed Charcot Restraint Orthotic Walker (CROW) was used in the treatment of 18 patients with diabetic neuroarthropathy involving the foot and ankle. Eight of these patients had no surgery before the use of the CROW. In ten patients, the device was used for prolonged immobilization after surgery for complications of neuropathic joint disease. The CROW is a rigid, custom, full-foot enclosure ankle-foot orthosis. It was used after an initial period of cast immobilization. The CROW effectively controls limb edema, returns the patient to ambulatory activities, and prevents significant progression of deformity. All patients rated their satisfaction with the device as good or excellent. The CROW is an attractive alternative to currently used methods to provide the prolonged immobilization and protection necessary for healing in neuropathic arthropathy.

Adult↗
PubMedDate not supplied

Modifications of eyebrow position with botulinum exotoxin A.

OBJECTIVE: To determine if clinically used botulinum exotoxin A (Botox) injections to the forehead and glabellar and crow's-feet regions result in modifications of eyebrow position. DESIGN: Prospective study. SETTING: Academic medical center in St Louis, Mo. SUBJECTS: Twenty-nine adult patients treated with botulinum exotoxin A injections for rhytids. INTERVENTION: The eyebrow position at 13 different sites was measured before injection and 2 weeks after treatment. The areas injected were based on patient preference and physician assessment. Of the 29 patients, 14 received injections into the glabella only and 15 received injections into the glabella and forehead, with or without treatment of the crow's-feet. RESULTS: In 29 patients at rest, we found no significant (P value range,.17 to.97) change in eyebrow position, except for a point depression at the right lateral eyebrow. The 15 patients who received injections into the forehead and glabella, with or without treatment of the crow's-feet, had no significant (P value range,.11 to.84) change in eyebrow position, except for a point of depression at the left medial eyebrow. Both groups exhibited eyebrow depression in the active state (eyebrow maximally elevated). CONCLUSIONS: Botulinum exotoxin A injections into the forehead and glabellar, and crow's-feet regions did not significantly change the resting eyebrow position. However, forehead injections contributed to eyebrow depression in the active state.

Adult↗
PubMedDate not supplied

Long-term effects of botulinum toxin type A (Botox) on facial lines: a comparison in identical twins.

OBJECTIVE: To evaluate the presence of imprinted facial lines in identical twin sisters, one of whom had received botulinum toxin type A (Botox) treatment in the forehead and glabellar region regularly for 13 years and one of whom had not. Crow's feet were also compared. METHODS: One twin received Botox in the forehead and glabellar region (approximately 2 to 3 times each year over the past 13 years) and in the crow's feet (twice in past 2 years). Her twin received Botox only twice (in the forehead and glabellar region, 3 and 7 years ago). RESULTS: Imprinted forehead and glabellar lines were not evident in the regularly treated twin but were evident in the minimally treated twin. Crow's feet were less noticeable when the regularly treated twin smiled (even at 7 months after treatment) than when the minimally treated twin smiled. Untreated facial areas (eg, nasolabial folds) showed comparable aging in both twins. Neither twin experienced adverse effects. CONCLUSIONS: Long-term treatment with Botox can prevent the development of imprinted facial lines that are visible at rest. Botox treatment can also reduce crow's feet. Treatment is well tolerated, with no adverse events reported during 13 years of regular treatment in this study.

Adult↗
PubMed1999

Testosterone modulates stimulation-induced calling behavior in Japanese quails.

Japanese quails have a variety of calling patterns depending on sex and age. Sexually mature adult males emit a characteristic crow which has a frequency-modulated acoustical trill element. Recently we reported that the intercollicular nucleus of the mesencephalon is the vocal neural system for producing the distress call in untreated chick and the crowing vocalization in testosterone-treated chick in Japanese quails. We postulated that the intercollicular nucleus could mediate this testosterone action on vocal behavior. The present study showed histologically that the intercollicular nucleus neurons of adult males have many more dendrites than those of females. The adult males produced the call with trill element with electrical stimulation of the intercollicular nucleus alone. Females produced the simple call, which has no frequency modulation, with electrical stimulation; 4 days after the testosterone treatment together with electrical stimulation, they also produced the call with trill element, the characteristic call of males. We suggest that the vocal neural system in the intercollicular nucleus is modulated by testosterone during development and produces the crow in males, and the intercollicular nucleus neurons in adults mediate the action of testosterone on vocal behavior. The crow of adult males could therefore be due to effects of testosterone on the neural mechanism in the intercollicular nucleus.

Animals↗
PubMed2001

Gait analysis after total hip arthroplasty with leg-length equalization in women with unilateral congenital complete dislocation of the hip--comparison with untreated patients.

Patients with Crowe Type-IV congenital dislocation of the hip (CDH) show significant clinical improvement after total hip arthroplasty (THA) because this surgery greatly reduces pain. Concomitant leg-length equalization in unilateral patients--a controversial procedure--theoretically should significantly improve these patients' ability to walk efficiently and comfortably. To understand the impact of leg-length equalization on these patients, we compared their gait parameters with those of untreated patients without pain but with leg-length discrepancy. Using a motion analysis system, three force platforms and computer calculation, the gait parameters during level walking of 22 women with unilateral Crowe Type-IV CDH were studied at an average of 58 months (27-98 months) following a successful cementless THA. The socket was placed in the best bone stock, which was close to the level of the true acetabulum. The leg-length discrepancy was equalized to within 2 cm in all patients. The Harris hip score averaged 94.8 (range, 88-100) at the time of the study. Nine women with untreated unilateral Crowe Type-IV CDH without major pain but with an average leg-length discrepancy of 4.7 cm (range, 2.5-6 cm) were also studied for comparison. The treated subjects (Group 1; THA and leg-length equalization) walked faster and had gait parameters with better bilateral symmetry than the untreated subjects (Group 2). We concluded that leg-length equalization in addition to THA in patients with unilateral Crowe Type-IV CDH significantly improves gait symmetry and efficiency.

Adult↗
PubMed1989

Models of positive and negative symptoms in schizophrenia: an empirical evaluation of latent structures.

The present investigation empirically evaluated three competing models of the relations between positive and negative symptoms in schizophrenia, namely the severity-liability model (Gottesman, McGuffin, & Farmer, 1987), Andreasen's unidimensional bipolar model (Andreasen & Olsen, 1982), and Crow's independent dual-process model (Crow, 1980a, 1980b). Using positive and negative symptom ratings based on 220 schizophrenic subjects, the results of a LISREL VI (Joreskog & Sorbom, 1984) confirmatory factor analysis revealed that Crow's model of positive and negative symptoms provided the best fit to the observed data among the three models. The severity-liability model provided a modest fit to observed data, and Andreasen's model fit the data poorly. Results are interpreted as supporting the validity of the positive and negative symptom distinction in schizophrenia and as providing substantive empirical support for Crow's independent dual-process model. The methodological advantages of confirmatory factor analysis in the specification and evaluation of theoretical models in experimental and developmental psychopathology are discussed.

Diseases in Twins↗
PubMed2006

The custom femoral component is an effective option for congenital hip dysplasia.

Although custom-designed femoral prostheses seem more likely to be successful than off-the-shelf components for treatment of patients with osteoarthritis secondary to congenital hip dysplasia, published short-term results of custom stems with a smooth surface or titanium proximal mesh pads are no better than results for off-the-shelf components. To determine whether cementless custom femoral components with sandblasted surfaces provide better results than other custom stems, we prospectively evaluated 77 consecutive patients (99 hips) with a mean age of 54 years who had osteo-arthritis secondary to congenital hip dysplasia. The mean followup was 9 years 3 months. Forty-seven hips were classified as having Crowe Type I deformity, 41 were classified as having Crowe Type II deformity, and 11 were classified as having Crowe Type III deformity. The average Harris hip score was 97.6 points at the last followup. Radiographically, 88 hips (89%) showed bone ongrowth, seven hips (7%) showed stable fibrous fixation, and four hips (4%) showed unstable fixation. There were two intraoperative femoral fractures and varus malpositioning of four stems in the hips with stable fibrous fixation and the unstable hips. One femoral component was revised 6 years postoperatively. Although the anatomic cementless custom-designed femoral components with sandblasted surfaces provided favorable results in patients with Crowe Types I, II, and III deformities, intra-operative technical errors prevented bone ongrowth fixation.

Adult↗