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Induction and catabolite repression of cellulase in Penicillium funiculosum.

The regulation of endoglucanase synthesis in Penicillium funiculosum is investigated using a method based on the viscosity lowering effect on carboxy methyl cellulose (CMC) by endoglucanase. Cellobiose (1 mg/L) causes induction, whereas glucose (5 g/L) does not repress the enzyme formation. Lactose (5 g/L) has no effect on the synthesis of cellulase. Avicel and cellulose powder (CP) are the best inducers of cellulase and xylanase activity. Both endoglucanase and xylanase activity were induced by CMC, whereas xylan induced only xylanase activity. The effect of protease on induction of cellulase activity is discussed.

Caseins↗

Relationship between sporulation and synthesis of mycobacillin and dipicolinic acid under condition of catabolite repression in Bacillus subtilis.

Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested, viz. glucose, pyruvate and glycerol 2-phosphate. However, these syntheses in the glucose-resistant mutants were not repressed by glucose and pyruvate but were repressed by glycerol 2-phosphate. The possible interrelationship between sporulation, dipicolinic acid and mycobacillin synthesis is discussed in light of these findings.

Antifungal Agents↗

Regulation of endopolygalacturonase gene expression in Botrytis cinerea by galacturonic acid, ambient pH and carbon catabolite repression.

The phytopathogenic fungus Botrytis cinerea produces a set of endopolygalacturonases (endoPGs) which are involved in the enzymatic degradation of pectin in plant cell walls. The endoPG-encoding genes of B. cinerea are differentially expressed when the fungus is grown in liquid culture on different carbon sources. A basic constitutive expression level was observed for two genes, Bcpg1 and Bcpg2, which encode basic isozymes. Galacturonic acid was shown to induce the expression of Bcpg4 and Bcpg6. Low pH of the culture medium resulted in induced expression of the Bcpg3 gene. Expression of the Bcpg5 gene was inducible; however the inducing factors could not be identified. Finally, galacturonic acid-induced expression of the Bcpg4 gene was repressed by the presence of more-favourable carbon sources, such as glucose.

Arabinose↗