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PubMedDate not supplied

[Induction of intracellular endonuclease synthesis in Serratia marcescens by agents suppressing DNA replication].

Endonuclease synthesis in Serratia marcescens was studied in the presence of agents selectively suppressing DNA biosynthesis: nalidixic acid, mitomycin, hydroxyurea and thymine limitation. All the agents suppressing DNA replication induced exocellular endonuclease biosynthesis irrespective of their action mechanism. The greatest inducing effect was exerted when the agents were added to cells in the late exponential phase. Endonuclease biosynthesis was induced 1-2 hours after adding the agent and was inhibited with chloramphenicol. The induction of exocellular endonuclease synthesis in Serratia marcescens by the classical inducing agents of a SOS response seems to be indicative of a Lex A regulated process.

DNA Replication↗
PubMed1991

Increase of hemolysin expression in Escherichia coli by cloned DNA sequences of Serratia marcescens.

A genomic library of Sau 3AI-generated Serratia marcescens DNA fragments was constructed in Escherichia coli 5K harbouring plasmid pANN202-312. Two clones capable of increasing the external hemolytic activity of the parental strain were isolated and characterized. The recombinant plasmids purified from these clones (designated pPSF29 and pPSH18) contained approximately 30 and 20 Kb, respectively, of Serratia DNA. Introduction of either plasmid in Escherichia coli caused a marked increase in hemolysin production and a concomitant decrease in cell viability.

Cell Membrane↗
PubMedDate not supplied

[Various physiological aspects of Serratia marcescens pigmented strains and their pigmentless variants with an elevated nuclease activity].

Some aspects of physiology of Serratia marcescens pigmentless variants with elevated nuclease activity were studied. The variants are characterized by a higher respiration rate when glucose, glycerol, inositol or maltose are used as an energy substrate, a higher respiration quotient, a lower growth rate, a lower economic coefficient, and a lower thermogenesis. The growth of Serratia marcescens pigmentless strains is presumed to be unbalanced.

Genetic Variation↗
PubMed1977

[Ultrastructural study of lipopolysaccharide and of polymyxin B-induced changes of the outer membrane of Serratia marcescens (author's transl)].

Electron micrographs of lipopolysaccharide (LPS) from Serratia marcescens which have been extracted with phenol/water, suspended in dist. water and subsequently negatively stained reveale round to ovoid particles besides singular ribbon-like structures. These structures are interpreted as collapsed LPS-strands of the outer membrane (OM). Fine structure investigations were carried out on strand-like structures which had been obtained by light alcalization of the particle suspension. Partial denaturation of LPS in ethylene-diaminotetracetic acid with polymyxin B (PB) gave rise to broad bends with periodic 180 degrees-torsions, indicating a helical structure. Chemically fixed LPS in phosphate buffer which were only partial transformed into LPS-strands, additionally revealed that a given LPS-strand consists of two electron-microscopic identical sub-strands which form a double helix. After short times of exposure to PB, negatively stained cells of Serratia marcescens show strand-like cell wall components on the cell surface consisting of longitudinal fibrils. In a further stage of denaturation, the strand-like structures form "projections" of the OM or are completely loosened. Based on a helical arrangement in the negative staining preparations as well as in the thin sections, they are identified as LPS-strands. Presumably, the LPS in the OM exists as contiguous strains. The development of the "double track"-aspect of the LPS in thin sections may be explained as a result of the projections of the helical longitudinal fibrils into the image plane.

Cell Membrane↗
PubMed1990

Growth depression of Serratia marcescens by plasmids that belong to incompatibility group P.

The IncP plasmids R702, RP4 and derivative plasmids including RP4::Mu cts were studied in two pigmented strains of Serratia marcescens. Maintenance of these plasmids resulted in reduced pigmentation and the growth rate of colonies was slower than the plasmid-free parent organisms. DNA inserts and deletions in RP4 altered both the pigmentation phenotype and growth rate of S. marcescens transconjugants and was inversely related to the change in molecular weight. The unrelated plasmids R446b and R46, although of comparable size to RP4, produced neither effect and Rts1, more than three times larger than RP4, produced no visible change in pigmentation phenotype and only a minimal decrease in growth rate. Integration of RP4::Mu cts into the Serratia genome restored pigmentation and growth rate to the same level as the plasmid-free parent. Partial induction at 43 degrees C restored the plasmid to a cytoplasmic state in a proportion of surviving colonies which were typically pale and slow growing.

Conjugation, Genetic↗
PubMed1990

Serratia marcescens mastitis in a dairy herd.

Serratia marcescens caused clinical mastitis in 5 cows and nonclinical mastitis in 21 cows of a 190-cow herd. Repeated bacteriologic culture of specimens from the cows, postmilking teat dip, environment, and equipment was performed. Serratia marcescens was not isolated from the dip, environment, or equipment. Progress of the infection in cows was monitored for 10 months. Some cows remained infected with S marcescens for at least 10 months. Economic loss estimates were based on Dairy Herd Improvement Association linear score reports. The average nonclinical loss was about $22/cow.

Animals↗
PubMed1989

[Antibiotic resistance plasmids from Serratia marcescens and their elimination by DNA-gyrase inhibitors].

We studied the effects of ciprofloxacin on 73 strains of Serratia marcescens. In the first place, we have tested their plasmidic content: 76% of Serratia marcescens strains contained plasmids by electrophoresis, and 29% of these plasmids were self-transmissible by conjugation. Secondly, we studied the plasmid stability with regard to ciprofloxacin. We obtained a spontaneous cure with 19% of plasmids, and ciprofloxacin, at very low concentrations (0.4 mg/l), increased the rate of cure and more efficiently than novobiocin, a compound used as a known curing agent.

Bacterial Typing Techniques↗
PubMed1988

[Nosocomial septicemia and pseudobacteremia caused by Serratia marcescens].

An epidemiological survey was carried out which included a dual epidemic of septicaemia and pseudo-bacteremia caused by Serratia marcescens. The survey enabled 15 septicaemias and 43 pseudobacteremias to be detected in a regional hospital between March and August, 1983. Two mishandlings were at the origin of the outbreak: citrated tube normally reserved for coagulation tests were severely contaminated by Serratia marcescens, and inaccurate samplings had been made. Once the mechanisms of contamination were found, specific preventive measures put an end to the epidemic. The authors insist on the need for uncontaminated tubes and citrate solutions and for the development of precise sampling methods which are essential to avoid the occurrence of pseudo-bacteremia or septicaemia. It is important to detect such epidemics at an early stage by an efficient control of nosocomial infections, thus avoiding their extension.

Blood Specimen Collection↗
PubMed1988

[Serratia marcescens and neonatal sepsis].

Authors review infections produced by Serratia marcescens which arose in our neonatal unit from 1982 to 1984, both inclusive. From a total of 4.353 newborns admitted, Serratia marcescens was isolated in 51 newborns; 26 of them exhibited a clinical picture of sepsis, remaining 25 being considered as contaminated. By comparing various characteristics of the contaminated and septic newborns, no difference was found related to sex, gestational age, prenatal pathology, type of delivery, Apgar score, birth-weight or time of diagnosis. Only difference between both groups was severity of intercurrent process and consequently, whether or not were they subject to previous antibiotic treatment and invasive therapeutic maneuvers. Authors conclude that perinatal factors seem to be not so important, as far as supporting a disseminated infection by this organism in concerned, in contrast to former observation by the others authors.

Enterobacteriaceae Infections↗
PubMedDate not supplied

[Non-pigmented Serratia: utility of hydrolysis of Tween 80 in its identification].

The ability of Serratia sp. to hydrolyze Tween 80 and the usefulness of this test for differentiating it from other Gram negative bacilli was studied, comparing it with the capacity of production of DNAsa by the same strains using the O-Toluidine blue method. The total number of strains assayed was 88. The sensitivity obtained was 83.9% and specificity 91.3%, with a positive predictive value of 83.9% and a negative predictive value of 91.2%. Using this method to differentiate only non-pigmented Serratia from Klebsiella sp. and Enterobacter sp. the sensitivity and specificity obtained was 100%. Concerning the cost, this method proved to be 3.86 times cheaper than the DNAsa one.

DNA, Bacterial↗
PubMed1977

Retention of pacemaker electrode complicated by Serratia marcescens septicemia. Removal with total cardiopulmonary bypass.

A case in which Serratia marcescens septicemia complicated the insertion of a transvernous pacemaker unit is reported. Appropriate antibiotic therapy and removal of the pacemaker electrode are two essential steps to achieve a complete cure in this stimulation. Open cardiotomy with total cardiopulmonary bypass provides a safe approach for withdrawal of an incarcerated electrode and is justified because of the lethal potential of systemic Serratia infections, particularly those superimposed on intracardiac prostheses.

Anti-Bacterial Agents↗
PubMed1977

Sequential hospitalwide outbreaks of resistant Serratia and Klebsiella infections.

Late in 1973 at the Nashville Veterans Administration Hospital, an intrusion of Serratia marcescens infections that were resistant to gentamicin sulfate and other antimicrobial agents occurred. This abated somewhat, only to be superseded by another wave of multiply-resistant infections due to Klebsiella pneumoniae beginning in the spring of 1974. Approximately 400 patients had substantial infections with these organisms during the 2 1/4 year period, imposing considerable morbidity and mortality. Due to the serious and lasting impact that these events imposed on patient care in our hospital, we sought explanations for the sequential infectious outbreaks. Both may have arisen because of the same persisting pressures favoring prevalence of multidrug-resistant bacteria. Indirect evidence including the sequential order of the outbreaks, similarity of antibiotograms, transferable multiple drug resistance from Serratia to Klebsiella, and possession of approximately equal molecular weight plasmids supported the notion that the two outbreaks were causally related.

Cross Infection↗
PubMedDate not supplied

[Correlation between the synthesis of extracellular proteases and the synthesis of the red pigment prodigiosin in Serratia marcescens].

A correlation has been established between synthesis of exocellular protease and synthesis of a red pigment prodigiosine by Serratia marcescens. Chloramphenicol, an inhibitor of protein synthesis, inhibits also synthesis of the pigment. Leucine, an inductor of synthesis of the exocellular protease by Serratia marcescens VI, induces also synthesis of the pigment. A mixture of 18 natural amino acids, asparagine and ammonium ions represses both synthesis of the enzyme and the pigment.

Chloramphenicol↗
PubMed1986

Immunization against experimental Pseudomonas aeruginosa and Serratia marcescens keratitis. Vaccination with lipopolysaccharide endotoxins and proteases.

Rabbits vaccinated with lipopolysaccharide endotoxins or with purified protease preparations from Pseudomonas aeruginosa and Serratia marcescens before corneal challenge with the viable bacteria exhibited significantly less corneal damage than rabbits not vaccinated with the bacterial products. However, the rabbits vaccinated with the lipopolysaccharide endotoxin preparations were significantly better protected than rabbits vaccinated with the bacterial proteases. Rabbits vaccinated with antisera raised against the proteases showed significantly less corneal damage than rabbits vaccinated with normal rabbit serum, and the passive protection was not significantly different than that elicited by active immunization against the bacterial proteases. The ability of the antiserum raised against the pseudomonas elastolytic protease to passively protect against severe corneal damage produced by experimentally induced pseudomonas keratitis was confirmed in mice. These findings support the idea that the bacterial endotoxins and proteases are virulence factors during the development of pseudomonas and serratia keratitis.

Animals↗
PubMed1978

[Resistance of Serratia marcescens to an etbylene oxide-methyl bromide mixture and the possibility of using it to control the effectiveness of disinfectant measures].

The authors studied the Serratia marcescens (strain No. 851) resistance to the okćbm mixture by the method of test objects in comparison with the vaccine virus (strain B-51) at a temperature of 20, 30, and 40 degrees C. Resistance of the mentioned bacteria to the okćbm mixture proved to be somewhat greater than that of the vaccine virus at 20 and 30 degrees C, whereas at 40 degrees C their resistance was found to be practically identical. This permits to use Serratia marcescens to control the efficacy of gaseous sterilization of materials.

Disinfectants↗
PubMed1978

[Increasing incidence of Serratia marcescens bacteraemia in intensive care patients (author's transl)].

The incidence of serratia marcescens in an intensive care unit was investigated in course of several years. After a trial of Cephalosporin-Gentamycin prophylaxis, infection and death due to serratia rose dramatically. The significance of decreased resistance to infection, hygiene regimes as well as mode of administration of antibiotics is related to selection of this rare microorganism causing septicemia.

Adolescent↗
PubMed1978

[Effect of glucose concentration on the biosynthesis of prodigiosin by serratia marcescens (author's transl)].

Serratia marcescens is an enterobacteria which produces a characteristic red pigment denominated prodigiosin. To study the effect of glucose on the kinetics of this secondary metabolite, cultures of Serratia marcescens S10 were incubated at 30 degrees C in the mineral medium GL, with glucose (2 g/l) as the carbon source. Prodigiosin production in relation to glucose consumption is studied, and parallel-wise, the effect of various concentrations of glucose on prodigiosin production. The kinetics data show the close correlation between glucose consumption and the synthesis of prodigiosin. This substrate inhibits the synthesis of pigment in cultures grown on solid medium GL with concentrations of glucose up to 15 g/l.

Animals↗
PubMed1978

[Transforming activity of plasmid R6K DNA on Serratia marcescens strain 20-10. The behavior of the plasmid in the transformants].

The authors described transformation of S. marcescens, strain 20-10, of the isolated R6K plasmide DNA. As demonstrated by centrifugation in cesium chloride gradient and electrophoresis in agarose, the plasmide was present in the transformants in the form identical to R6K in E. coli K12. Analysis of the transforming activity of R6K plasmide from Serratia and E. coli K12 strains with a complete and defective restriction system showed S. marsescens, strain 20-10, to possess specific system of restriction and modification. In studying beta-lactamase activity and Serratia and E. coli strains ampicillin and streptomycin resistance revealed differences in the phenotypical expression of the plasmide signs in the heterologous and homologous host.

DNA, Bacterial↗