Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Pyronine”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 361 records · Page 20Linked to original sources
PubMed1998

[Homocysteine-induced neural tube defects in chick embryos and protection of folic acid].

To reveal whether homocysteine(HCY) will be able to induce neural tube defects (NTDs) and possible teratogenic mechanisms, and identify the interventional effects of folic acid and vitamin B12 on HCY-induced NTDs, 1274 chick embryos treated with L. D-HCY (0-16 mumol/embryo) were detected by the methods of teratogenic trial, scanning electron microscopy(SEM), Nile blue sulfate vital staining, TdT-mediated dUTP nick end labeling(TUNEL) methylgreen-pyronine special staining for showing nucleic acid, and interventional assay of folic acid and VB12 in different developmental stage. Results showed that HCY could induce teratogenesis in chick neurulation and organogenetic period, in dose-response relationship (P < 0.0001). The main forms of NTDs were exencephaly, cranioschisis and spina bifida. It has been first found that HCY could target excessive apoptosis of cells of embryonic nervous system, which consists with the sites of embryos developing NTDs. HCY could inhibit chick yolk sac vessel formation and blood circulation, and damage amniotic membrane. Under SEM it was observed that HCY resulted in abnormal ultrastructures, for example cavernous damages, atrophied microvilli and so on. After the injection of 5 micrograms/embryo folic acid, the teratogenicity of HCY (8 mumol/embryo) was significantly antagonized, the occurrence rate of NTDs was down from 43.5% to 0(P < 0.05). However, vitamin B12 (1 microgram/embryo) did not obviously attenuate the teratogenicity of HCY in day 6 chick embryos. These data prove that HCY per se causes dymorphogenesis of the neural tube. Apoptosis may play an important role in the etiopathology of NTDs. Folic acid can effectively prevent from the homocysteine-induced NTDS. Causes of homocysteine-induced NTDs may result from interaction or combination of different mechanisms.

Animals↗
PubMed1998

[The diversity of human hematopoietic stem/progenitor cells: IX. Cell-cycles kinetics related macromolecules DNA, RNA and protein contents in CD34+ hematopoietic cells of human bone marrow].

DNA, RNA and PRO comprise the bulk of macromolecules in cells, which have been proven to play an important role in regulating cell cycle transverse capacity, cell division, growth, and size. Simultaneous analysis of these moieties could provide more comprehensive and accurate information on cell cycle kinetics. In this study, DNA, RNA and PRO contents related to cell cycle kinetics in CD34+ hematopoietic cells of human bone marrow were measured to understanding the cell cycle kinetic features in CD34+ hematopoietic cells. For this reason, CIMS-100 immunomagnetic isolator, a novel isolation system, was used to enrich efficiently CD34+ hematopoietic cells from human bone marrow. The purity of enriched CD34+ hematopoietic cells determined by both FACS and APAAP staining is ranging from 90%-95%. Cellular DNA, RNA and PRO were stained with fluorochromes propidium iodide, pyronin Y and fluorescein isothiocyante respectively The fluorescence intensities reflecting the DNA, RNA and PRO content of individual cell were analyzed in FACS can by different excitation wavelengthes. DNA, RNA and PRO contents in CD34+ hematopoietic cells were far lower than these of bone marrow mononuclear cells, only being 34 +/- 3% (DNA), 48 +/- 21% (RNA) and 62 +/- 14% (PRO) of BMMNCs respectively. Collectively, these data combined with previous results from both ours and others indicated that CD34+ hematopoietic cells are indeed an unique cell population, not only in reconstitute of hematopoietic and immunological functions, but also in cell cycle kinetics. This is, to our knowledge, the first detailed report on the analysis of DNA, RNA and PRO contents related to cell cycle kinetics in CD34+ hematopoietic cells. And the results provide more direct evidence that the majority of CD34+ hematopoietic cells are in resting state.

Antigens, CD34↗
PubMed1999

Focal necrotizing endometritis: a clinicopathologic study of 15 cases.

From routine sign-out of endometrial biopsy specimens, a group of 15 endometria were identified that have a characteristic histologic pattern of inflammation that is not included in present classifications of endometritis. All but one of the women were premenopausal, and all presented with abnormal vaginal bleeding. The lesion is characterized by a patchy, focal inflammation, usually composed of lymphocytes with a variable number of neutrophils and rare macrophages centered around endometrial glands and extending into the glandular lumen with disruption and partial or subtotal necrosis of the endometrial glandular epithelium. These foci were widely dispersed, never confluent, and could be overlooked easily. Plasma cells were not found in any of the endometrial specimens despite methyl green pyronine staining of the samples. Based on the histologic characteristics of this process we have designated it focal necrotizing endometritis. The clinical significance, if any, of focal necrotizing endometritis is currently unknown.

Adult↗
PubMed1999

[Relationship between homocysteine-induced apoptosis and teratogenesis in developing avian embryo].

It has been hypothesized that homocysteine(HCY) can induce apoptosis at the organogenesis-stage of chick embryos and which can be the relationship between HCY and congenital defects of neural tube and heart. Exogenous D, L-HCY (4-8 mumol/embryo) was injected into the place near brain and the yolk of the day 4 embryos. Then they were harvested 2 days later and examined by flow cytometry, histopathology, electronic microscope, in situ end labeling of DNA strand break and methylgreen-pyronine double staining. The change of plasma HCY level of day 10 embryos was also inspected by HPLC analysis. The HCY injected at different position of embryos could result in heart and neural tube defects, which included ectopia cardis, hydropericardium, endocardial cushion defect, encephalocele, microcephaly, abnormal flexions and so on. HCY could apparently disturb embryonic cell cycle, suppress the synthesis of DNA and RNA of embryonic cells. Excessive apoptotic cells in brain and heart were observed in HCY\|treatment groups. It was discovered that mitochondrial damage could be described as an early event in cellular apoptosis of chick embryos. After 8 mumol of HCY was placed on the inner shell membrance, there was an obvious increase of plasma HCY concentration with a peak value of 80.58 mumol/L in 120 min, and followed by declining. On the other hand, 5 micrograms of folic acid given simultaneously with HCY could sharply curtail the rise in plasma HCY. It is concluded that HCY is a cytotoxin, inducing over apoptosis of embryos in some areas, which is coincident with the pathologic changes of neural system and heart. These findings show that there is a close relationship among HCY, apoptosis and birth defect in developing chick embryos. Mitochondrial dysmorphosis may be an important early event of apoptosis in embryos after exposure to HCY.

Animals↗
PubMed2003

Angiostatin(4.5)-mediated apoptosis of vascular endothelial cells.

Angiostatin, a proteolytic cleavage product of plasminogen, acts via a selective, yet poorly understood mechanism to potently inhibit angiogenesis (M. S. O'Reilly et al., Cell, 79: 315-328, 1994). Vascular endothelial cell proliferation assays revealed that angiostatin(4.5), a naturally occurring human isoform consisting of plasminogen kringle domains 1-4 and most of kringle domain 5 (G. A. Soff, Cancer Metastasis Rev., 19: 97-107, 2000), dose dependently reduces cell number despite the presence of a potent stimulus of proliferation. Flow cytometry using the vital dyes Hoechst 33342 and Pyronin Y revealed that approximately 40% of both control and angiostatin(4.5)-treated cells were in the proliferative phase, indicating that cell cycle progression is not impaired by exposure to angiostatin(4.5). Both bovine aortic endothelial cells and human umbilical endothelial cells were shown to undergo apoptosis in response to angiostatin(4.5). Caspases-3, -8, and -9 activation, specified by cleavage of fluorophore-conjugated specific peptide substrates, revealed a cascade of caspase activation that peaks at 36 h of angiostatin(4.5) treatment. Angiostatin(4.5) exposure induced release of cytochrome c from mitochondria in a caspase-dependent manner, but a pan-caspase inhibitor, zVAD-fmk, blocked cytochrome c release. Overall, these data indicate that human angiostatin(4.5) may function in vivo to block blood vessel formation by specifically inducing vascular endothelial cells to apoptose in a process likely involving both the intrinsic and extrinsic apoptosis pathways.

Angiostatins↗
PubMed1977

Histochemical observations on the mycetomes of Pyrilla perpusilla Walker.

Histochemical studies of mycetomes and mycetocytes of Pyrilla perpusilla show PAS positive material, what can suggest the presence of glycogen. The mycetocytes failed to stain with alcian blue and methyl green pyronin "Y" indicating the absence of mucopolysaccharides and RNA respectively. The mycetocytes show positive congo red. Millon and Mercury bromophenophenol blue staining thereby proving that they contain glycoprotein, tyrosine and proteins. Positive reaction with Sudan black may show the presence of lipids and lipoproteins.

Animals↗
PubMed2002

[Optimalization for preparation and expansion of ex vivo human umbilical blood cells for transplantation].

This work was aimed at finding optimal conditions for isolation and storage of hematopoietic cells from umbilical cord blood and checking the usefulness of ex vivo expansion in a serum-free medium. Mononuclear cells were isolated either by density gradient centrifugation (Ficoll-Histopaque or Gradisol L) or by hypoosmotic lysis of erythrocytes and granulocytes in ammonium chloride. Toxicity of metabolic dyes (Hoechst 33342, Rhodamine 123, Pyronine Y) used in flow cytofluorimetry and the effects of freezing in dry ice or in a mechanical freezer with and without a methanol bath were compared. The influence of ex vivo expansion of cells in a serum-free medium on their proliferative potential and apoptosis was investigated. In comparison with ammonium chloride, density gradient centrifugation both in Ficoll-Histopaque and Gradisol L offered superior and consistent results as to cell yield, vitality and proliferative potential. Hoechst 33342 demonstrated the highest toxicity with CD34+ cells, disqualifying this dye for transplantation purposes. Mechanical freezing with prior methanol bath was found to be the safest method of storage. The concentration of TGF beta released by cells subjected to expansion ex vivo in a serum-free medium was markedly elevated and correlated with the percentage of apoptotic cells. Furthermore, the proliferative potential of cells deteriorated as a result of expansion.

Blood Preservation↗
PubMedDate not supplied

Immunomorphological reactivity of the intrapulmonary lymphoid tissue.

Young albino rats were repeatedly stimulated by a pulverized suspension of living pneumococci and others by intramuscular injection of killed microbes emulsified in complete Freund adjuvant. The bronchus associated lymphoid nodules increase in size and number; the proximal nodules contain one more follicle with germinal centers formed of pyroninophilic lymphoblasts and plasma cells at the periphery. The follicular organization and the pyronine - plasma cells differentiation point out the ability of these nodules to elaborate a local immune response to antigenic stimulation.

Animals↗
PubMed2004

Expression of progesterone receptors in bovine corpus luteum during pregnancy.

The expression of progesterone receptors and matrix ribonucleic acid in 37 corpora lutea in 1-7 month's pregnant cows was investigated. Corpora lutea were obtained from slaughtered animals. Progesterone receptors expression was confirmed using biotin-streptavidin immunohistochemistry. Matrix ribonucleic acid expression was studied using pyronin staining by Unna-Brashe method. The expression of progesterone receptors was the highest in the luteal cells of the one-month pregnancy corpus luteum. It decreased significantly (p<0.05) from the third to the fourth month of pregnancy, but there was no significant difference in the expression of progesterone receptors from the first to the second and from the second to the third month of pregnancy. Also, there was no significant difference in the expression of progesterone receptors from the fourth to the fifth month and from the fifth to the sixth month of pregnancy. The expression of matrix ribonucleic acid also decreased during pregnancy, but gradually, with no significant monthly differences. It decreased significantly (p<0.05) from the sixth to the seventh month of pregnancy only. Our study shows that both indices - progesterone receptors and matrix ribonucleic acid decrease in the steroidogenic luteal cells during pregnancy and that a strong linear correlation (r=0.88) exists between these indices.

Animals↗
PubMed1990

[Hybridization analysis of nucleic acids using stained latex].

Method of the latex hybridization analysis has been developed: after hybridization of NA-target with biotinylated probe visualization of hybrids was carried out using latex particles, containing fluorescent dye pyronin G and coated with streptavidin. Due to encapsulation of the fluorescent dye in polymer particles sensitivity of the analysis was increased by several orders of magnitude in comparison with methods, using fluorescently labelled probes. Possessing a number of advantages, the method yields to none of any other methods of NA hybridization analysis in sensitivity.

Latex↗
PubMed1991

Copper tetrapyridino 'phthalocyanin' (Cuprolinic blue) differs in shape from the palladium and platinum analogues, and this affects staining of polynucleotides.

Palladium and platinum homologues of the copper-containing tetrapyridinotetraazaporphin cationic dye Cuprolinic blue were prepared. The affinities of all three dyes for a series of polyanions, with and without nucleotide bases, were assessed using the CEC (critical electrolyte concentration) approach, and expressed as molarities of MgCl2. Whereas Cuprolinic blue bound to RNA with much greater avidity than to DNA, the Pt and Pd analogues showed very high affinity for both. Patterns of affinities for non-nucleotide polyanions showed no differences. 1H and 13C NMR spectra showed that, although the Cu compound was planar, the Pt and Pd homologues must be non-planar. Taken together with previous findings, on e.g. methyl green and pyronin, these results suggest that DNA finds it easier to accommodate pairs of out-of-plane aromatic rings than RNA in stable complexes. CEC analyses can, as demonstrated here, uncover quite subtle differences between molecular recognition patterns of very closely related reagents.

Coloring Agents↗
PubMed1991

[Changes in the lipid and protein components of thymus cell membrane during lipid peroxidation].

Nonenzymatic lipid peroxidation in thymus cell plasma membranes was studied. The composition of lipid and protein components, intensity of fluorescence of the membrane probes (1-anilinonaphthalene-8-sulfonate, 4-dimethylaminochalcon, eosin, pyronin and rhodamine), fluorescence polarization of tryptophan residues of membrane proteins and quenching by acrylamide of intrinsic fluorescence of proteins were determined. Induction of lipid peroxidation by the Fe(2+)-ascorbate system caused changes in the composition and structure of lipids. This was paralleled with changes in the structural-dynamic organization of membrane proteins, transition of some peripheral proteins to the water phase and increased solubilization of integral proteins by Triton X-100.

Animals↗
PubMed1991

Characterizations of and interactions between bile ductule cells and hepatocytes in early stages of rat hepatocarcinogenesis induced by ethionine.

Numerous hepatic cell lineage pathways have been proposed for the development of hepatocarcinogensis induced by chemical carcinogens in rats. The roles of bile ductule cells and hepatocytes in the development of carcinogenesis were investigated using light and electron microscopic procedures to detect differences in morphology and in the phenotypic expression of antigens that are associated with each cell type. In early stages of hepatocarcinogenesis (4-10 weeks after initiation of feeding of a choline-deficient ethionine containing diet), both bile ductulelike (BDL) cells and hepatocytes were seen in mitosis. At the light microscope level, BDL cells showed intense cytoplasmic pyronin (RNA) staining and were positive for the antigens defined by monoclonal antibody 270.38 (bile ductule cells and "oval" cell marker) and glutathione-S-transferase (Yp isoform), whereas hepatocytes were positive for the antigens defined by monoclonal antibodies 270.26 and 258.26 (liver parenchymal cell markers), catalase activity (peroxisome marker) and adenosine triphospatase activity (bile canalicular marker). The authors frequently encountered BDL cells and hepatocytes in close proximity. Ultrastructural examination showed extensive plasma membrane appositions between a subset of BDL cells and hepatocytes. Desmosome structures, tight junctions, microvilli interdigitations and ATPase-positive bile canalicularlike structures were present along the contiguous plasma membrane domains of BDL cells and hepatocytes. Many of the BDL cells attached to hepatocytes were also attached to other BDL cells that had retained a basal lamina. In many cases, BDL cells connected to both hepatocytes and other BDL cells were no longer completely surrounded by basal lamina and had acquired a dual polarity as a consequence of their sharing apical and lateral membrane domains with both BDL cells and hepatocytes. BDL cells showed increased numbers of microperoxisomes (catalase positive organelles) and numerous free ribosomes. Hepatocytes showed a prominent development of the smooth endoplasmic reticulum, a feature prominent in hepatocytes within hyperplastic nodules. Since BDL cells and hepatocytes proliferate and BDL cells and hepatocytes develop intercellular junction sites, the authors propose that both cell types in early stages of carcinogenesis have the capacity to enter the cell lineage pathway leading to the development of hepatocarcinoma. Furthermore, the finding that BDL cells and hepatocytes form multiple attachment sites at the level of the plasma membrane, suggests the possibility that at some stage convergence of separate hepatic cell pathways may occur.

Animals↗
PubMed1987

[Cytochemical study of the cysts of the sarcosporidian Sarcocystis bovicanis. I. Nucleic acids, polysaccharides, lipids and proteins].

A light microscopic study of S. bovicanis cysts and cyst stages has been carried out, in addition to morphological characterization of cysts. At least two types of cyst stages could be distinguished--merozoites and metrocytes. The light microscopic differentiation of the third type--the intermediate cells--from merozoites seems to be rather difficult especially when non-dividing cells are examined. Merozoites (zoites) much varied in size, and besides the usual parasitic cells with the terminal nuclei, cells with the central ones were recognized. Since the classical Feulgen reaction did not give sufficient results when establishing DNA distribution, its modification with a fluorescent agent Auramin O was used. The latter provided excellent results showing numerous chromatin granules in the nucleus, no distinct nucleoli being determined. Gallocyanin--chromalum method and methyl green--pyronin staining for DNA and RNA demonstrated a poor staining of the nucleus contrasting with an intensive coloration of cytoplasmic RNA and associated high level protein synthesis. The PAS reaction revealed numerous polysaccharide granules in the cytoplasm of zoites. On cryostat sections a certain PAS positive layer was distinguished around the cyst in the muscle tissue which did not disappear even after a long term amylase treatment. Even more intensively stained was the pre-cystic muscle after cytochemical test for general protein using amido black and coomassie blue. It does not seem unlikely that some metabolic changes may occur in the host cell harbouring the cyst. Several methods for lipid detection in cyst stages with Fat red, Oil red O and Sudan black B were used with negative results.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

Cytopathology of alpha chain disease involving the central nervous system and pleura.

Alpha chain disease, a lymphomatous disorder characterized by the synthesis and secretion of an abnormal IgA immunoglobulin devoid of light chains, involves mainly the gastrointestinal tract. This paper presents the cytologic and histologic findings in two cases of alpha chain disease involving the central nervous system and pleura. Most of the cell populations in the cerebrospinal fluid (CSF) and pleural fluid resembled immature plasma cells (immunoblasts); many of these cells were degenerated, with well-preserved plasma cells seen more rarely. While the definitive diagnosis of alpha chain disease depends on immunochemical analysis of serum proteins, cytology can play a role by the identification of malignant cells in CSF and pleural fluid specimens. A positive staining of such cells by the methyl green pyronin reaction may permit the correct diagnosis to be suggested.

Adult↗
PubMed1989

[Isolated symplasts and stromal syncytial buds of the normal human placenta at 6 to 14 weeks of pregnancy].

Formation of isolated symplasts (IS) ("deported trophoblast", according to English nomenclature) and stromal syncytial buds (SSB) has been studied in 10 normal patients at pregnancy of 6-14 weeks by means of the indirect immunoperoxidase method. Monoclonal antibodies against beta-subunit of the human chorionic gonadotropin (beta-HCG), serial paraffin slices, stained with hematoxylin-eosin, semithin slices, stained with toluidin blue-pyronine and scanning electron microscopy have been used. A great amount of syncytiotrophoblastic processes and IS, analogous by their structure to syncytiotrophoblast are revealed. Presence of beta-HCG in the syncytiotrophoblast and its absence in the cytotrophoblast are proved. Concentration of beta-HCG in the IS is higher than in the syncytiotrophoblast of the villi. Maximal concentration of beta-HCG in the SSB is described for the first time. The functional role of the IS and SSB is discussed in the system mother-placenta-fetus.

Cell Fusion↗
PubMed1989

[Effects of cyproterone acetate on mouse submandibular gland].

It is recognized that the submandibular gland (SMG) of mice is one of the target organs for androgen, and a specific androgen-binding protein (androgen receptor) has been found in the cytosol fraction of this gland. Androgen participates in the production of histochemically and biochemically detected components of this gland through this receptor. Cyproterone acetate (CPA) is a synthetic progestogen which possesses antiandrogenic properties with some synandrogenic action. However, the action of CPA on the SMG has not been well defined. In the present study, the effects of CPA on the granular duct (GD) of the mouse SMG, which is the site of androgen action, were investigated using histometric, histochemical, and biochemical techniques. For histometric and histochemical experiments, normal adult male mice were treated with CPA (20, 40 and 80 mg/kg, S.C.) every second day for 5-30 days. In other experiments, castrated (CAS) and castrated-adrenalectomized (CA) mice were prepared, and they were given an injection of testosterone propionate (TP; 20 mg/kg, S.C.) and/or CPA (20 and 200 mg/kg, S.C.) every second day for 10 days. After the final administration, SMG weights were determined and the size of the GD in the SMG was measured by light microscopy using a micrometer. The sections of SMG were stained with methylgreen-pyronin, bromophenol blue, and p-dimethylaminobenzaldehyde in order to detect RNA (RNA staining), total protein (BPB staining), and granules (Try staining) in the GD, respectively. The intensity of each staining was measured by the method of Sato et al. For biochemical experiments, the mice with testicular feminization (Tfm), which are genetically deficient in androgen receptor, and genetically normal male (X/Y)-castrated mice were used. Both Tfm and X/Y-castrated mice were injected with CPA for histological experiments. After the administration period, the SMG's of both Tfm and X/Y-castrated mice were isolated and esteroprotease activity was determined by the method of Trautschold. The SMG of non-administered X/Y-castrated mice was homogenized in Tris-HCl buffer. And cytoplasmic extracts of SMG's from non-administered X/Y-castrated mice were prepared for detection of inhibitory effect of CPA on specific binding pattern of 3H-methyltrienolone (R1881) by the sucrose density gradient method.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMedDate not supplied

[Effects of met-enkephalin on the testis. II. Histochemical study].

We performed a histochemical study using the Alcian blue-PAS staining method (for mucopolysaccharide), vitamin C, Sudan black (for lipids), and methyl green-pyronine (for nucleic acid). For the study, we utilized 105 male Wistar rats weighing 280-300 gms. Thirty rats comprised the control group, and 75 comprised the study group. Rats in the study group received a single, acute intracardiac dose of met-enkephalin (100 microliters of 50% met-enkephalin solution) and were sacrificed at 15, 30 and 60 minutes following injection, or a chronic intramuscular dose (50 microliters of 40% met-enkephalin solution). We observed that met-enkephalin caused histochemical changes in the rat testis, as evidenced by the accumulation of mucopolysaccharide (early in the study), cytoplasm lipid degeneration, changes in protein synthesis, and a fall in vitamin C stores (in seminal epithelium cell lines, as well as Leydig cells). These changes were more marked in the chronically than in the acutely-treated rats. The foregoing findings demonstrate that enkephalins (endogenous opiates) can cause profound metabolic changes in the rat testis that affect all its metabolic elements [proteins, lipids, polysaccharides and active substances (vitamins...)].

Animals↗