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DETERGENTS: EFFECTS ON THE CHEMICAL SENSES OF THE FISH ICTALURUS NATALIS (LE SUEUR).

Two types of detergents, one easily oxidized and the other not, damage the chemoreceptors of yellow bullheads, Ictalurus natalis (le Sueur) exposed to concentrations of 0.5 parts per million in the surrounding water. This concentration is considerably lower than that at which sublethal damage has been detected previously. Histological examination reveals erosion of the taste buds; electrophysiological methods and observations of swimming and feeding behavior reveal impairment of receptor function. Affected fish do not fully recover after 6 weeks in detergent-free water.

Animals↗

COOLING DEVICE FOR USE WITH A SONIC OSCILLATOR.

A cooling cell is described that facilitates maintenance of biological materials at low temperatures during prolonged sonic treatment. Using this cell and a Branson S-75 Sonifier, I examined temperatures and the release of protein and enzyme activity from suspensions of Saccharomyces cerevisiae. With the Sonifier at full power, it was possible to maintain cell temperature within 9 C of the cooling-bath temperature, and to disrupt 10% (w/v) suspensions of S. cerevisiae in 10 min.

Bacteriological Techniques↗

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Electrodiagnosis↗