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PubMed1951

Granulation tissue.

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Granulation Tissue↗
PubMed1989

Effects of prednisolone and human epidermal growth factor on angiogenesis in granulation tissue of gastric ulcer induced by acetic acid.

In an attempt to elucidate the role of granulation vessels in the healing of gastric ulcer, healing and angiogenesis in granulation tissue of acetic acid ulcers were studied in rats. In addition, the effects of prednisolone and synthetic human epidermal growth factor (EGF) on angiogenesis and ulcer healing were investigated. The newly formed granulation vessels in the ulcer base were measured by means of a carmine dye infusion method. Prednisolone, administered subcutaneously at 40 mg/kg/day, significantly decreased angiogenesis in the ulcer base on the 10th day after ulcer production, and on the 30th day ulcer healing was found to be significantly delayed. In contrast, angiogenesis was significantly increased, and ulcer healing was enhanced by intragastric administration of 100 micrograms/kg/day of EGF. With combined administration of prednisolone and EGF, angiogenesis was significantly increased compared to that observed with prednisolone treatment alone. The authors conclude that suppression of angiogenesis by prednisolone is a delaying factor in gastric ulcer healing and that exogenous EGF promotes ulcer healing, partly through restoration of angiogenesis.

Acetates↗
PubMedDate not supplied

[Dynamics of nucleic acid synthesis in the granulation tissue of skin wounds in the rat].

Dynamics of nucleic acid synthesis was studied in granulation tissue, harvested within 2-11 days after an injury of rats with full-layer skin wounds, simultaneously with evaluation of the morphometric patterns of the tissue. Synthesis of nuclear DNA exhibited a wave-shaped dynamics. The intervals between the maximal values of the DNA synthesis constituted 3 days. Within the early steps of wound healing the intensive incorporation of 3H-thymidine into mitochondrial DNA was found, which appears to occur due to active mitochondriogenesis. In dynamics of total and nuclear RNA contents two peaks were observed, which did not coincide in time, thus suggesting the wave-shaped increase in biosynthetic processes in cells.

Animals↗
PubMed1989

Two- and three-dimensional ultrastructure of endothelium and pericyte interdigitations in capillary of human granulation tissue.

Two- and three-dimensional electron microscopic observation in the immature capillary of the human granulation tissue revealed cytoplasmic interdigitations (CID) between the endothelium and the pericyte. These were composed of a cytoplasmic projection and indentation, and there was a gap space without basement membrane-like components between the two cell membranes. Plasmalemmal vesicles were frequently found locating beneath and/or attached to the cell membranes at the indentated side of the interdigitation. Two kinds of cytoplasmic interdigitation, each having a characteristic configuration, were demonstrated. The CID which was composed of a cytoplasmic projection from the endothelium to the pericyte had a dull-shaped cytoplasmic projection, while the CID which was composed of a cytoplasmic projection from the pericyte to the endothelium had a slender and long finger-like configuration.

Capillaries↗
PubMed2000

Apoptosis and expression of Bax/Bcl-2 proteins in pyogenic granuloma: a comparative study with granulation tissue and capillary hemangioma.

BACKGROUND: Pyogenic granuloma (PG) has been regarded as a florid expression of granulation tissue (GT) proliferation and shows distinct biological behavior compared to conventional GT. PG has not been examined from a standpoint of apoptosis. METHODS: PG (15 cases), GT (14 cases) and capillary hemangioma (CH, 8 cases) were compared using in situ 3'-tailing reaction (ISTR), a histochemical method for identifying apoptotic cells, and immunohistochemistry for Ki-67, Bcl-2 and Bax proteins, in order to clarify and involvement of apoptosis in the difference of biological characteristics between PG and GT. RESULTS: PG showed significantly lower ISTR-labeling indices than GT and CH and more frequent Bcl-2/Bax expression than GT, whereas Ki-67-labeling indices were variable from case to case and did not show any significant differences among any groups. CONCLUSIONS: It is consequently suggested that the low apoptotic rate in PG is closely related to its characteristic rapid growth and is regulated by Bcl-2 family proteins.

Apoptosis↗
PubMed1997

Overexpression of granulocyte-macrophage colony-stimulating factor induces pulmonary granulation tissue formation and fibrosis by induction of transforming growth factor-beta 1 and myofibroblast accumulation.

We have previously reported that transfer to rat lung of the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene leads to high expression of GM-CSF between days 1 and 4 and granulation tissue formation followed by an irreversible fibrotic response starting from day 12 onward. In the current study, we investigated the underlying mechanisms. We found that GM-CSF overexpression did not enhance production of tumor necrosis factor-alpha in a significant manner at any time after GM-CSF gene transfer. However, the content of transforming growth factor-beta 1 in bronchoalveolar lavage fluid was markedly induced at day 4 and appeared to be maximal around day 7 and remained high at day 12. Macrophages purified from bronchoalveolar lavage fluid 7 days after GM-CSF gene transfer spontaneously released significant quantities of transforming growth factor-beta 1 protein in vitro. After peak transforming growth factor-beta 1 production was the emergence of alpha-smooth muscle actin-rich myofibroblasts. Accumulation of these cells was most prominent at day 12 within the granulation tissues and they were still present in fibrotic areas between days 12 and 24 and diminished markedly afterward. Thus, we provide the first in vivo evidence that tumor necrosis factor-alpha may be dissociated from participation in a fibrotic process in the lung and GM-CSF may play a more direct role in pulmonary fibrogenesis at least in part through its capability to induce transforming growth factor-beta 1 in macrophages and the subsequent emergence of myofibroblast phenotypes. This GM-CSF transgene lung model is useful for a stepwise dissection of both cellular and molecular events involved in pulmonary fibrosis.

Actins↗
PubMed1978

The inhibitory effects of a dermal extract upon granulation tissue.

Injection of a water-soluble dermal extract into adult rats resulted in depression of DNA synthesis in granulation tissue at 3 different sites of injury, but not in epithelium. This suggests that connective tissue proliferation may be controlled in part by a chalone-like mechanism.

Animals↗
PubMed1977

[DNA synthesis by granulation tissue fibroblasts under conditions of stimulation of the wound process].

The changes in the DNA synthesis in the granulation tissue fibroblasts in the wounds of adult animals given potassium orotate and in controls were studied. For this purpose a daily autoradiographic examination of the wound areas were conducted in the both groups of animals. It appeared that under the effect of potassium orotate (0.2 ml of 2% solution) there was more frequent involvement of fibroblasts into the DNA synthesis; under conditions of the wound process stimulation there occurred an increase of the proliferative activity of these cells.

Animals↗
PubMedDate not supplied

[Fibronectin fragmentation unmasks the activity stimulating DNA and RNA biosynthesis in granulation tissue cells in vitro].

Human blood plasma fibronectin decreased slightly the incorporation of precursors into nucleic acids of granulation tissue culture cells. A slight fragmentation of fibronectin, where the fragments with 180-200 kD molecular mass were developed, led to occurrence of the activity 2-fold stimulating the DNA synthesis. After more effective proteolysis using plasmin and trypsin the stimulating effect of fibronectin fragments on synthesis of nucleic acids maintained and constituted 165 +/- 12% and 127 +/- 7% for DNA and RNA, respectively.

Animals↗
PubMed1993

Short report: effect of sucralfate on angiogenesis in granulation tissue of acetic acid-induced gastric ulcers in rats.

We investigated the effect of sucralfate on angiogenesis in granulation tissue of gastric ulcers induced by acetic acid in rats using the carmine dye method. Intragastric administration of sucralfate at a dose of 500 mg/kg twice daily for 9 days significantly accelerated ulcer healing and significantly increased the extent of angiogenesis in the ulcer base on the tenth day after ulcer induction. As we reported previously, intragastric administration of cimetidine at a dose of 100 mg/kg once daily for 9 days decreased the extent of angiogenesis on the tenth day. However, combination treatment using sucralfate and cimetidine accelerated ulcer healing significantly, without altering the extent of angiogenesis. It is concluded, therefore, that the treatment with sucralfate may be effective in peptic ulcer disease from the standpoint of angiogenesis in the ulcer base.

Animals↗
PubMed2006

Reducing ischaemic damage in rodent ovarian xenografts transplanted into granulation tissue.

BACKGROUND: Anti-cancer therapies frequently lead to ovarian damage and impaired fertility. To preserve fertility, cryopreservation and subsequent transplantation of the ovaries have been suggested. One of the challenges in ovarian graft transplantation is overcoming the initial ischaemic damage that depletes a significant fraction of the oocyte pool. METHODS AND RESULTS: Follicular survival in ovarian grafts was examined by magnetic resonance imaging (MRI) and fluorescence microscopy in a model system in which rat ovaries were transplanted into nude mice. Transplantation into angiogenic granulation tissue created during wound healing shortened the ischaemic period by 24 h and significantly increased the pool of healthy primordial follicles and the perfused area of the transplanted grafts. Functional blood vessels were detected within the grafts as early as 2 days after transplantation. Gain of function was demonstrated both by growth of the grafts and by the hormonal influence on the host uteri. CONCLUSION: Implantation of ovarian grafts into an angiogenic granulation tissue improved graft vascularization and follicular survival. This procedure/treatment may be used for reducing the ischaemic damage in ovarian transplants, thus prolonging graft functionality and increasing the yield of oocytes that can be easily recovered for fertilization.

Animals↗
PubMed1980

Cell composition of granulation tissue in defibrinogenated rabbits.

The influence of systemic defibrinogenation on cell composition and capillary formation in sponge-induced early granulation tissue was studied in rabbits. The tissue was studied histologically and the content of RNA, DNA and Hb was determined. Inflammatory cells were identified and quantified using histochemical and biochemical techniques. An accumulation of PMN-leucocytes was found after defibrinogenation whereas the amount of fibroblasts and capillaries was reduced.

Afibrinogenemia↗