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[Plastic replacement of the abdominal wall by uncoated carbon cloth. An animal experiment on the rabbit (author's transl)].

Carbon cloth is a new biomaterial which is suitable for the replacement of ligaments and connective tissues, because of its biological and physical properties. In 30 rabbits a defect was cut into the abdominal wall. In 20 the defect was covered by an implanted carbon cloth. The other 10 animals were used as controls. After implantation wound healing was excellent. No herniation could be observed in the group with the implants. After 3 months histological examinations revealed the ingrowth of collagen fibres into the carbon cloth. It had become a strong and resilient abdominal wall replacement. A physical-strength test showed stability of the newly formed abdominal wall with good anchorage to normal tissue.

Abdominal Muscles↗

Inactivation of influenza A virus by gentian violet (GV) and GV-dyed cotton cloth, and bactericidal activities of these agents.

Recently we have heard warnings of an outbreak of a highly pathogenic avian influenza virus (H5N1). Although, to prevent such infections we must prepare anti-viral drugs and type-specific vaccines against influenza, we need various simple and effective protection methods, such as the use of face masks for public health. Also, in any consideration of bacterial infections, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococci (VRE), and multidrug-resistant Pseudomonas aeruginosa (MDRP) also pose serious concerns which must be addressed. I examined the antiviral activity of gentian violet (GV) and GV-dyed cloth against the influenza A (H1N1) virus. Time-kill studies were carried out, and the virus titer was determined based on the 50% tissue culture infective dose (TCID50). The minimum inhibitory concentrations (MICs) of GV against bacteria were also determined, and the killing activities of the GV-dyed cloth were judged from viable cell counts. GV immediately killed the influenza A virus and this was confirmed by electron microscopy. Moreover, cloth dyed with a combination of GV and copper showed not only excellent antiviral activity but also prominent bactericidal activities.

Animals↗

Clothing fasteners for long-term-care patients: evaluation of standard closures and prototypes on test garments.

For the elderly long-term-care patient it is of great psychological and practical importance to be able to dress and undress without assistance. However, the design and position of clothing fasteners often lead to a restriction of the functional capacity of the patient. The aim of this study was to facilitate a more independent daily life for long-term care patients. New fasteners were developed in accordance with a user-oriented product development process where a detailed knowledge of the final users and their capacity and limitations, problems, needs and wishes form the basis for further development. In an initial experimental study (I) the practical use of different standard clothing fasteners (button, hook and eye, nylon pressure tape and zipper) was evaluated. Different fasteners and positions are favourable to different patient groups. Wrist angle in different hand positions is crucial for the function of fasteners for persons with reduced hand function. User demands on clothing fasteners were specified and prototypes developed. In a subsequent study (II) an adapted oval button and a 'finger strap' alternative to hook-and-eye were designed and evaluated, together with a standard button. The oval button in combination with a vertical buttonhole improved the function for most patients, and the front position for the fasteners was superior to a diagonal or lateral position on the chest. The finger strap was of advantage to patients with hemiplegia and joint complaints but was difficult for many of the subjects to understand, being a technical innovation.

Journal Article↗

Restriction to movement in fire-fighter protective clothing: evaluation of alternative sleeves and liners.

The purpose of this study was to evaluate alternative designs and liner configurations in fire-fighter protective clothing, or 'turnout gear', to determine the restriction to wearer movement imposed by each. The independent variables were: (1) two alternative sleeve designs (i e, a 'traditional' sleeve design and a prototype sleeve design, featuring additional gusset width and altered armseye position) plus a station uniform worm without any protective clothing and/or equipment; (2) three liner configuration variations (i e, a 'traditional' liner configuration, incorporation of one additional liner, and incorporation of two additional liners); and (3) wearing or not wearing an SCBA (self-contained breathing apparatus). The dependent variables for this study were: (1) range of movement in four upper body joints; and (2) a semantic differential scale to evaluate wearers' subjective evaluation of each protective ensemble. Nine male subjects were used. For each of the four joint movements measured (i e, shoulder flexion/extension, shoulder adduction/abduction, shoulder rotation, elbow flexion/extension), a Leighton Flexometer was strapped to the subject at the appropriate body location. The subject was instructed to take the body position indicated. A reading was taken, then the subject was asked to move the body segment to the fullest extent possible in the direction indicated by the researcher. A second reading (representing range of movement) was taken. This procedure was repeated three times for each movement. After the test, subjects were instructed to fill out a semantic differential scale which described their subjective evaluations of the clothing/ equipment configuration. Results showed greater wearer range of movement in the elbow area for the prototype sleeve design over the more traditional sleeve design. Incorporation of additional liners resulted in higher wearer acceptability for the turnout coats than when these liners were not used. As expected, use of an SCBA was extremely restrictive to mobility, and made the protective ensemble less acceptable to wearers.

Journal Article↗

Use of detergents in the preparation of Salmonella samples for enzyme immunoassay on polymyxin-coated polyester cloth.

Heating Salmonella cells in the presence of detergents such as deoxycholate or Triton x-100 greatly increased the sensitivity of an enzyme immunoassay for lipopolysaccharide antigens using polymyxin-coated polyester cloth as the solid phase for antigen capture. The presence of a 100-fold excess of E. coli lipopolysaccharide in the test sample did not affect the detection of the Salmonella lipopolysaccharide. The sensitivity of the assay using deoxycholate-heat treatment of the antigens followed by detection on polymyxin-coated cloth was equivalent to that of an assay using antibody-coated polyester cloth for antigen capture.

Deoxycholic Acid↗

Long-term results of the cloth-covered Starr-Edwards ball valve.

BACKGROUND: Between June 1968 and March 1977, Starr-Edwards cloth-covered ball valves were exclusively implanted, in a total of 74 patients at our institution, on a routine basis. METHODS: The retrospective postoperative follow-up rate was 84.5%, for up to 36 years in mitral valve patients (982.5 patient-years); and 81.3%, for up to 34 years in aortic valve patients (282.0 patient-years). Among 66 operative survivors, 20 patients required reoperation due to valve-related complications. Mortality and morbidity after valve replacement was reviewed, and excised valves were examined. RESULTS: Survival rates after 10, 20, and 30 years were 74.6%, 64.1%, and 31.2%, respectively, after mitral valve replacement, and 62.5%, 50.0%, and 43.8%, respectively, after aortic valve replacement. Freedom from all valve-related complications, respectively after 10, 20, and 30 years, was 70.5%, 55.9%, and 46.4% after mitral valve replacement, and 56.2%, 37.5%, and 31.2% after aortic valve replacement. Cloth wear or pannus overgrowth was observed in all excised prostheses. Remarkable orifice tear was observed in mitral valves that were more than 20 years old. Pannus overgrowth on the studs contributed to prosthetic regurgitation in the older valves. Concomitant valve procedures were frequently required for valve-related complications or other aggravated valve lesions in the mitral position during the follow-up period. CONCLUSIONS: Early diagnosis of valve dysfunction and the decision to reoperate are important to improve the long-term results for surviving patients who have received a cloth-covered Starr-Edwards valve, especially in the mitral position.

Adolescent↗

Studies on removal of metribuzin, bromacil, 2,4-D and atrazine from water by adsorption on high area carbon cloth.

The removal of pesticides such as metribuzin, bromacil, 2,4-d and atrazine from aqueous solutions was studied by adsorption on high area carbon cloth. The adsorption process was followed by in situ UV-spectrophotometric technique in a specially designed adsorption cell. Spectroscopic data of the pesticides were determined in separate experiments. The extent of adsorption was quantified by calculating the amount of adsorbate adsorbed per unit area of the carbon cloth and the percentage coverage at the carbon-cloth surface. The order of extent of adsorption of the pesticides studied was found as metribuzin < 2,4-D < bromacil < atrazine. The adsorption process was found to follow a first-order kinetics and the rate constants were determined. The competitive adsorption of pesticides was also examined by carrying out the adsorption process from a solution of equimolar mixture of bromacil and metribuzin.

2,4-Dichlorophenoxyacetic Acid↗

Structural and ionization effects on the adsorption behaviors of some anilinic compounds from aqueous solution onto high-area carbon-cloth.

The adsorption of anilinic compounds; aniline, p-toluidine, 1-napthylamine and sodium salt of diphenylamine-4-sulfonic acid from solutions in H2O, in 1 M H2SO4 or in 0.1 M NaOH onto activated carbon-cloth was studied by in situ UV spectroscopy. A specially designed adsorption cell was used for this purpose. The adsorption processes were found to follow first-order kinetics and the rate constants were determined. The pH at the point of zero charge of the carbon-cloth surface was measured as 7.4. The highest rates and extents of adsorption were observed from H2O solutions for aniline, p-toluidine and 1-napthylamine and from solution in 1 M H2SO4 for the sodium salt of diphenylamine-4-sulfonic acid. The adsorption behaviors of these four anilinic compounds in the three solutions (in H2O, in 0.1 M NaOH and in 1 M H2SO4) were explained in terms of electrostatic and dispersion interactions between carbon-cloth surface and the anilinic species. The adsorption isotherm data for the anilinic compounds were derived at 30 degrees C and treated according to Langmuir and Freundlich models. The Freundlich model was found to represent the experimental isotherm data better than Langmuir model.

Adsorption↗

Adsorption of phthalic acid and its esters onto high-area activated carbon-cloth studied by in situ UV-spectroscopy.

The adsorption behavior of phthalic acid and its three esters dimethyl phthalate, diethyl phthalate and diallyl phthalate onto high-area activated carbon-cloth was studied by in situ UV-spectroscopic technique. The effect of ionization of phthalic acid on its adsorption was examined by carrying out the adsorption process in three media; water, 1 M H(2)SO(4) and 0.005 M NaOH. Maximum adsorption was observed in 1 M H(2)SO(4) and almost no adsorption in 0.005 M NaOH. These results were discussed in terms of electrostatic and dispersion interactions between the adsorbate species and the carbon-cloth surface taking the point of zero charge (pH(pzc)) of the carbon-cloth into account. The adsorption process for the phthalate species studied was found to follow the first-order rate law, and the rate constants were determined. The isotherm data for the adsorption of phthalic acid and its esters were derived experimentally and fitted to Langmuir and Freundlich isotherm equations. Both equations were found to represent the experimental isotherm data almost equally well.

Adsorption↗

Aortic and mitral valve replacement with cloth-covered Braunwald-Cutter prosthesis. A three-year follow-up.

From February, 1971, through February, 1974, heart valves of 510 patients were replaced with cloth-covered Braunwald-Cutter prostheses. The data indicate that cloth encapsulation of the prosthetic cage is associated with a reduction in thromboembolic complications, particularly for mitral valves. Cloth wear on the distal strut, however, is not prevented by use of a Silastic poppet and appears to be a typical finding in aortic valves recovered or inspected after 3 months. Excessive poppet wear has also been noted in the aortic position; poppet embolization has occurred on 2 occasions, and a third patient was found, at the time of reoperation for periprosthetic leak, to have opppet wear sufficient to permit embolization. Although there have been no reports of clinical malfunction of the mitral prosthesis at the time of this writing, further long-term observation of these patients appears warranted.

Adolescent↗

Evaluation of an office- and hospital-based intervention to increase the use of cloth diapers.

To evaluate an office-based educational intervention to promote the use of cloth diapers, we randomly assigned parents of 193 infants either to the intervention group or to a control group. The intervention consisted of physician advice and written material on cloth and disposable diapers at the 2-week office visit. Midway through the study, one of the birth hospitals switched to the exclusive use of cloth diapers. The results indicate that the diapering decisions of parents can be influenced by practices in hospitals and by office-based physician and nurse counseling.

Adult↗

A cloth strip bioreactor with immobilized glucoamylase.

Glucoamylase was immobilized on polyethylenimine (PEI)-coated cotton cloth by adsorption followed by cross-linking with 0.2% glutaraldehyde in the presence of starch. Optimal adsorption of the enzyme was seen when cloth treated with 2% PEI was contacted with the enzyme for 50 min. pH and temperature optima profiles were not changed appreciably on immobilization. However, the bound enzyme exhibited a higher thermal stability. The enzyme-bound cloth strips were used in a specially designed bioreactor for the continuous hydrolysis of starch. The reactor could be operated for over 21 days retaining about 70% of the original activity. An operational temperature of 45 degrees C was found to be optimal.

Adsorption↗

Severe hemolysis due to cloth wear 23 years after aortic valve replacement on a Starr-Edwards ball valve model 2320.

Despite iron substitution therapy, a patient developed severe hemolytic anemia 23 yr after insertion of a cloth-covered Starr-Edwards model 2320 aortic valve prosthesis. The prosthesis showed no sign of significant dysfunction. Upon removal, it showed extensive cloth wear on the inner surface of all three struts; one strut was completely denuded of its cloth covering. Hemolysis immediately resolved after replacement with a St Jude aortic prosthesis.

Anemia, Hemolytic↗

Performance profile of the Starr-Edwards aortic cloth covered valve, track valve, and silastic ball valve.

OBJECTIVE: The Starr-Edwards aortic ball valve has passed 30 years of clinical follow-up. A detailed account of the long-term performance from a large series could thus give valuable guidance in managing patients who are still alive, depict the total remaining life-span after aortic valve replacement (AVR) for the average patient, and set a record yet to be matched by modern disc valves. METHODS: A detailed follow-up to a maximum of 31.1 years was performed for 717 patients who underwent their first AVR during 1965-1993 with a Starr-Edwards silastic ball valve (N = 355), a cloth covered valve (N = 164) or a track valve (N = 198) with a total of 7254 patient-years at risk. RESULTS: Patients who received a silastic ball valve were older (average 60 vs. 58 years), had more endocarditis (9%) and more secondary kidney failure (24%) preoperatively than the other patients. The three valve types did not differ as regards long-term survival or freedom from complications and only 15% of late deaths were related to the valve. For the silastic ball valve cumulative freedoms at 10 and 25 years were 59 and 20% from all deaths (crude survival), 85 and 80% from thromboembolism, 87 and 70% from bleeding, 98 and 94% from endocarditis, 96 and 95% from redo AVR and 68 and 51% from all valve related complications joined. There were no instances of structural failure apart from wear of the cloth covering the cage struts of the cloth covered valves. Incidences of haemolysis (0.10%/patient-year) and valve thrombosis (0.06%/patient-year) were low for the silastic ball valve. Analysis of relative survival for the silastic ball valve indicated excess mortality relative to a matched background population only during 1st and 13th postoperative year. Apart from heart related factors and age, independent incremental risk factors for mortality and the various complications included, not valve type, but valve size index (valve size divided by body surface area) < or = 13 mm/m2. CONCLUSIONS: The Starr-Edwards aortic ball valves, not least the currently available silastic ball valve, are durable through the remaining life time of the patients and able to secure near normal age and sex specific survival provided valve and patient size mismatch is avoided.

Adult↗

Laboratory evaluation of disposable and reusable disinfectant cloths for cleaning food contact surfaces.

A comparison of five methods of cleaning Formica surfaces contaminated with bacteria dried in milk has been carried out. A standardized procedure was developed, and impression plates were found to be at least as sensitive as a swab-rinse method for detecting bacteria on the surfaces. The most satisfactory results were obtained with one type of disposable alcohol-impregnated wipe and with a detergent/hypochlorite solution applied with paper. A reusable cloth impregnated with disinfectant initially performed well against all test organisms, but was less reliable against Staphylococcus aureus and Streptococcus faecalis, after the cloth had been used and rinsed several times. The importance of introducing methods to reduce the high risk of cross-contamination presently associated with the use of wiping cloths in catering premises is stressed.

Animals↗

Dermal exposure assessment to benzene and toluene using charcoal cloth pads.

Charcoal cloth pads have been used to assess volatile chemicals on the skin in a laboratory setting; however, they have not yet been applied to measure dermal exposure in occupational settings. This study aimed at evaluating whether charcoal pads can be used to assess dermal exposure to benzene and toluene in workers of a petrochemical plant. Inhalation and dermal exposure levels to benzene and toluene were assessed for workers of a petrochemical plant performing different jobs. Benzene uptake was assessed by determining S-phenylmercapturic acid in workers' urine samples. Dermal exposure levels on the charcoal pads were adjusted for ambient air levels of benzene and toluene by subtracting the amount of benzene or toluene measured in personal air from the amount of benzene or toluene measured on the charcoal pad. In general, measured external and internal exposure levels were low. The estimated contribution of the dermal route to internal benzene exposure levels was less than 0.06% for all jobs. Toluene personal air concentrations and benzene and toluene dermal exposure levels differed statistically significantly between job titles. For benzene, differences between jobs were larger for adjusted dermal exposures (maximum 17-fold, P = 0.02) than for inhalation exposures (maximum two-fold, P = 0.08). Also for toluene, although less clear, differences between jobs were larger for adjusted dermal exposures (maximum 23-fold, P = 0.01) as compared to inhalation exposures (maximum 10-fold, P = 0.01). Charcoal pads appeared to measure dermal exposures to benzene and toluene in addition to ambient air levels. Future studies applying charcoal cloth pads for the dermal exposure assessment at workplaces with higher dermal exposure to organic solvents may provide more insight into the biological relevance of dermal exposure levels measured by charcoal cloth pads. In addition, the design of the dermal sampler might be improved by configuring a dermal sampler, where part of the sampler is protected against direct contact and splashes, but still permeable for the gas phase. This design would most likely result in a better ability to correct for airborne concentrations at a given body location.

Administration, Cutaneous↗

Poly(ethyleneimine)-immobilized-cloth enzyme immunoassay for the detection of Salmonella lipopolysaccharide.

Poly(ethyleneimine) (PEI) was immobilized on non-woven polyester cloth and examined for application on a simple, rapid and economical "cloth enzyme immunoassay (CEIA)" which was developed originally as polymyxin-CEIA for the detection of Salmonella lipopolysaccharide (LPS). PEI-cloth regardless of the PEI molecular weight, but with the amine group contents of 0.1 to approximately 0.35 meq/g immobilized either in a physisorption-like or chemisorption-like manner, adsorbed LPS rapidly, preferentially and effectively. The captured LPS was then able to be detected qualitatively and quantitatively as an antigen by enzyme immunoassay. PEI-CEIA had a detection limit for Salmonella LPS of 10 ng/ml, which was equivalent to 1.6 x 10(5) cell/ml and was ten times more sensitive than polymyxin-CEIA. It was possible to detect Salmonella LPS in the presence of a 100-fold excess of E. coli LPS. PEI-CEIA was found to be more sensitive and much easier to carry out than polymyxin-CEIA but had the same advantages as polymyxin-CEIA.

Adsorption↗

Surface sampling for endotoxin assessment using electrostatic wiping cloths.

OBJECTIVES: Much of the cost of exposure assessment for studies of residential cohorts is in scheduling and travel time for field staff. One way to reduce costs is to simplify methods such that subjects can sample their own residence. Analysis of settled dust is being widely used for assessment of exposures to allergens, lead and pesticides and can also be used for endotoxins. While vacuum sampling is the most common surface sampling method, wipe sampling has the advantage that it can be readily performed by the resident when convenient and samples can then be mailed to researchers. Thus, we evaluated the feasibility of wipe sampling for endotoxin environmental assessment using electrostatic wipes with or without the use of disposable examination gloves. METHODS: Multiple lots of six types of commercial wipes and eight types of gloves were extracted and analyzed for endotoxin content using the kinetic chromogenic Limulus amebocyte lysate assay. Wipes were compared across brands, between lots, within lots, between pairs depending on proximity to cardboard packaging, and in wipe tests with or without gloves. Collected dust samples of known concentration were also tested in spiking assays for endotoxin recovery. RESULTS: The most striking finding was the high variability of endotoxin contamination of both wipes and gloves across brands and between various lots. The content of endotoxin in unused gloves ranged from <1.5 to 5810 endotoxin units (EU). The range for unused wipes was 3.6-87.8 EU. Surfaces of equal loading and area were sampled using three types of cloths that had low initial endotoxin contamination. The cloths were very good at collecting dust and endotoxin could be assayed from aqueous extracts of the wipes. Samples collected using cloths with bare washed hands yielded higher endotoxin loading per mass of collected dust versus samples collected wearing endotoxin-free gloves. This demonstrated additional endotoxin loading from the subject's hand. CONCLUSION: This study shows that wipe sampling while wearing medical gloves can be an effective method for collecting and assessing endotoxin on surfaces, so long as each lot of wipes and gloves have been tested and determined to be low in endotoxin.

Air Pollutants↗