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At least 37 records · Page 2Linked to original sources
PubMed1975

The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests.

In a collaborative investigation in which 4 laboratories took part, the Rose Bengal Test (RBT), Serum Agglutination Test (SAT) and 4 different Complement Fixation (CFT) techniques were evaluated in selected cattle for the diagnosis of bovine brucellosis, by comparing the results they gave with the bacteriological examination of a selection of lymph nodes taken from the same animals at slaughter. The RBT correctly classified all but 1 of 79 culture-positive cattle, but was more often positive in culture-negative animals than the other tests. The RBT may be most useful as a screen test. 11% of the culture-positive cattle had SAT titres below 100 iu and almost 4% of them had less than 30 iu. However, the SAT was more effective in cattle vaccinated with the 45/20 vaccine. The CFT, in 1 of 4 techniques used, identified all of the culture-positive cattle at a serum dilution of 1/4 or above and was considered to be far superior to the SAT as a diagnostic test, except perhaps in cattle vaccinated with the 45/20 vaccine.

Agglutination Tests↗
PubMed1978

[Diagnostic value of agglutination reaction with buffered Brucella antigen stained with rose bengal].

A specific buffered antigen has been obtained, employing a method developed by the authors, stained with Bengal rose and intended for performing a fast agglutination reaction to confirm brucellosis. The antigen produces a clear and demonstrative agglutination reaction with positive sera. Practically, the test is readily carried out, and can be made a routine both in every serologic laboratory and for investigations under field conditions. The reaction produced with this antigen can determine dependably the epizootic status on a farm or in the herd. The diagnostic value of the antigen is essential, especially with swine. Besides, it shows a wide a diagnostic scope for brucellosis with all species of animals (97--98 per cent).

Agglutination Tests↗
PubMed1979

Suitability criteria for compartmental analysis of the plasma clearance curve of exogenous cholephils. 131I Rose Bengal fulfils these criteria.

Hepatic clearance of exogenous cholephils involves at least three transfers : uptake by the liver, reflux from hepatocytes into the plasma, and intracellular transport followed by active secretion in bile. Compartmental analysis may be applied to the plasma disappearance curve in order to calculate the fractional transfer rates. The 131I Rose Bengal (131I RB) fills the requirements of such an analysis, contrary to bromsulphalein and indocyanine green, which have been used until now. 131I RB does not undergo enterophepatic cycle and is not metabolized by the liver. It behaves, in the preparation used, like a homogeneous substance, remains stable in the serum as long as 25 hours after its injection and may be bound completely with human albumin in vitro prior to administration : under these conditions, the material which is cleared by the liver is homogeneous. The use of tracer instead of loading doses, avoid any toxic effects on transport systems and results in plasma and liver concentrations which are negligeable in comparison with the Km of membrane transport, and are compatible with first order kinetics.

Chromatography↗
PubMed1979

Hepatic storage and biliary excretion of rose bengal in the rat.

The distribution of intravenously administered rose bengal (RB) depends on its dose. At a low dose (10 mg/kg), RB can be found almost solely in the liver and plasma. However, at higher doses (from 25 up to 200 mg/kg) the amount of RB found in extra-hepatic tissues gradually increases. In this experiment the hepatic transfer maximum of RB amounted to 146 micrograms/kg/min. By increasing the dose from 10 to 200 mg/kg, the hepatic concentration of RB also approached a maximum (1250 micrograms/g). The storage capacity of the liver, however, did not limit the transfer maximum of RB.

Animals↗
PubMed1977

[Differentiation of non-specific positive brucellosis reactions using an antigen stained with rose bengal].

A Brucella buffered antigen, stained with Bengal rose by a method of the authors, was obtained. It showed a high distinguishing capacity with regard to the nonspecific agglutinations after Huddleson and Wright at a negative complement-fixation test for brucellosis with sera from cattle, pigs, sheep, and horses. Such differentiation, however, proved to be incomplete and for sera of different animals varied within the range of 34 to 0.0 per cent.

Agglutination Tests↗
PubMed1979

RNA synthesis in growing and stationary cells of a culture of Scarlet Rose. Disproportionate synthesis of ribosomal subunits in the stationary state.

RNA synthesis has been investigated in resting and growing cells of a culture of Scarlet rose. The rates of messenger RNA (mRNA) and ribosomal RNA (rRNA) synthesis are five- and ten-fold higher, respectively, in the growing culture. In stationary phase cultures, newly synthesized 26S and 18S rRNA do not appear in the cytoplasm in equimolar amounts. Rather, the 26S/18S ratio of [3H]-uridine labeled rRNA of stationary cells ranged from 0.9 to 1.3 while the ratio of the corresponding fraction from growing cells was 1.6 to 2.0. A similar result was obtained when cells were labeled with [3H-CH3] methionine. Pulse chase experiments demonstrated that the nascent pre-rRNA in resting cells could be chased into polysomes. These data are interpreted to indicate that a major part of the regulation of rRNA synthesis in stationary cells is at the level of the processing of the rRNA transcript.

Cell Cycle↗
PubMed1979

[Interrelationship between glucoside-bound and free alcohols during ether oil formation in rose petals].

Some data on the dynamics of free and glucoside-bound monoterpenic and aromatic (beta-phenylethyl) ethers content and the changes in the beta-glucosidase activity in rose petals at different stages of the flower development and on the kinetics of enzymatic hydrolysis of these glucosides are presented. The phase specificity of beta-glucosidase coinciding with the maximal accumulation of glucoside-bound and free alcohols is revealed. The data obtained suggest that the formation of glucosides may precede the accumulation of corresponding free alcohols of terpenic and aromatic origin.

Alcohols↗
PubMed1978

[Biosynthesis of beta-phenylethanol in rose petals].

Biosynthesis of beta-phenylethanol is studied in a cell-free rose petals extract. This compound found to be synthesized via two intermediates: either trans-cinnamic acid, or phenylacetaldehyde. Subsequently beta-phenylethanol is glucosodized into its transport form, beta-phenylethanol- beta-D-glucoside. A scheme of beta-phenylethanol and its beta-D-glucoside is given.

Acetaldehyde↗
PubMed1979

A clinical comparison of 99mTc-diethyl-iminodiacetic acid, 99mTc-pyridoxylideneglutamate, and 131I-rose bengal in liver transplant patients.

The relative merits of three radiopharmaceuticals for evaluating liver transplant patients were determined in paired studies. In 86% of studies both 131I-RB and 99mTc-PG gave similar information for differentiation of hepatocellular disease and billary tract obstruction. 99mTc-PG probably demonstrates the biliary tract and small intestine better early after injection (8%); 131I-RB is probably better in showing the colon at 24 hours when intestinal activity is not seen by 1 hour (6%). 99mTc-diethyl-IDA is superior in all respects when compared to 99mTc-PG. The blood retention method (20 min./5 min.) showed that none of the radiopharmaceuticals was a reliable indicator of hepatocyte function when compared to total serum bilirubin.

Biliary Tract Diseases↗