Automated detection of mulberry bodies in urinary sediment for non-invasive Fabry disease screening.
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Fabry disease↗
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Results for “object detection”
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Early detection of chronic simple glaucoma in general practice.
Chronic simple glaucoma, also known as open-angle glaucoma, is the cause of blindness in about 13 per cent of people on the Blind Register, and 40 per cent of these have had no treatment before registration. Patients over the age of 60 are most liable to the disease and it is rare under the age of 40; there is often a family predisposition. I carried out a routine test for the disease in 300 patients over 60 years of age in my general practice with the object of detecting chronic simple glaucoma at an early stage. Anyone with an intraocular pressure of over 23 mm of mercury in either eye was referred to a consultant ophthalmic surgeon for further investigation. Seven patients (2.3 per cent of those examined) were confirmed as suffering from chronic simple glaucoma.
Age Factors↗
Genomic characterisation of ST233 Pseudomonas aeruginosa co-producing KPC-2 and VIM-2 in Northeastern Brazil during the COVID-19 pandemic: Evidence of independent horizontal acquisition events.
BACKGROUND: Dual-carbapenemase-producing Pseudomonas aeruginosa poses a major therapeutic and epidemiological challenge worldwide, yet systematic data on KPC and VIM co-production in Brazil remain limited. The COVID-19 pandemic intensified antimicrobial use, a period temporally associated with increased carbapenemase detection globally. OBJECTIVES: To characterise the molecular epidemiology and resistance profiles of KPC and VIM co-producing P. aeruginosa isolates from Brazil (2019-2023). METHODS: Between 2019 and 2023, 1489 multidrug-resistant P. aeruginosa isolates were screened by multiplex PCR for carbapenemase-encoding genes. Co-producing isolates underwent pulsed-field gel electrophoresis (PFGE) for clonal profiling, followed by whole-genome sequencing (WGS) for high-resolution phylogenomic analysis. Antimicrobial susceptibility testing and plasmid characterisation using next-generation sequencing platforms were also performed. RESULTS: Forty-two isolates (2.8%) harboured both blaKPC-2 and blaVIM-2, with detection occurring exclusively between 2020 and 2023, temporally coinciding with the COVID-19 pandemic. PFGE identified eight distinct clonal groups, providing evidence for independent horizontal gene transfer (HGT) events, whilst WGS confirmed all isolates as the high-risk ST233 lineage. Chromosomally integrated blaVIM-2 within class 1 integrons predominated; 2 isolates carried dual chromosomal copies. Plasmid-borne blaKPC-2 was identified across heterogeneous replicons (43.3-430.1 kb), suggesting multiple independent acquisition events. All co-producing isolates displayed extensive drug resistance, retaining in vitro susceptibility only to cefiderocol and colistin. CONCLUSIONS: ST233 co-producing KPC and VIM, represents a high-risk resistance phenotype of epidemiological significance. Divergent genomic architectures suggest active horizontal dissemination across diverse genetic backgrounds rather than clonal expansion, highlighting the need for enhanced surveillance and infection control strategies.
Bacterial genomic characterisation↗
[Methods of detecting the agent of melioidosis in objects of the external environment and other material].
On the basis of literature data and personal investigations the authors elaborated a scheme of detection of the causative agent of melioidosis in the objects of external environment, including fluorescent microscopy of crude material, seeding on meat-peptone agar with crystal violet, seeding material treated with penicillin on fluid medium with subsequent transfer on solid Levin medium and infection of animals, seeding on a medium with beta-alanine and neonmycin and infection of sensitive animals with crude material.
Animals↗
Clinical pharmacology in normal volunteers of praziquantel, a new drug against schistosomes and cestodes. An example of a complex study covering both tolerance and pharmacokinetics.
The tolerance of Praziquantel (2-cyclohexylcarbonyl-1, 3, 4, 6, 7, 11b-hexahydro-2H-pyrazino-[2, 1-a]isoquinoline-4-one) in oral doses of 1 X 20 mg/kg, 1 X 50 mg/kg, 3 X 10 mg/kg and 3 X 25 mg/kg body weight (tau = 4 h) was tested in a complex study involving 36 healthy volunteers. In addition to the usual assessment of clinical chemistry, haematology, coagulation physiology, urinalysis, clinico-physiological examination including EEG, and medical examination, clinico-psychological parameters were also recorded and special neurological investigations were performed. No clinically relevant changes were found in any of the laboratory parameters, nor in the medical-neurological or clinico-physiological examinations. Based on a few clinico-psychological parameters and subjective comments, the largest daily dose tested (3 X 25 mg/kg = 75 mg/kg) produced a slight, transient disturbance in general well-being, which was barely detectable on objective clinical examination. The pharmacokinetic behaviour was dominated by rapid metabolism and pronounced first-pass metabolism of praziquantel, which greatly limits the value of results obtained by GC analysis of unchanged drug in serum. The peak concentration in serum was reached after 1--2 h, and the elimination half-life for the period 2--8 h was 1--1.5 h.
Adult↗
Gamma-ray spectrometry of humans at the University of Utah.
A human total-body counter was designed and built with two 20 X 10 cm NaI (Tl) crystals suspended over an "isoresponse surface" upon which the subject reclines. This surface is curved from head to knee and from left to right, so that a gamma-ray emitting object is detected with equal efficiency when placed anywhere upon it. The positioner and detectors are housed in a low background enclosure constructed of steel 31 cm thick with a graded inner lining of lead + cadmium + copper. Calibration of the system was accomplished by administering trace amounts of various radionuclides to 48 human subjects of various sizes, ranging in age from 4--80 years. Counting rates per retained muCi at 0.53, 0.66, 1.53, and 2.75 MeV (83Rb, 137Cs, 42K, and 24Na) were determined as a function of body size and were compared with counting rates per muCi of corresponding emitters centered in a polyethylene cylinder of radius 10.3 cm. Limits of detection, corresponding to three times the standard deviation of a 50 min background, were 170 nCi 90Sr (via Bremsstrahlung X-rays), 0.78 nCi 131I, 0.48 nCi 83Rb, 0.52 nCi 137 Cs, 4.9 nCi 40K (or 5.8 g of natural potassium) and 1.7 nCi 222Rn.
Body Height↗
Detection of color in rotating objects by infants and its generalization over changes in velocity.
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Age Factors↗
Whole exome sequencing and cluster analysis reveal that EPB41L4A mutation may trigger tooth agenesis.
OBJECTIVE: To detect and analyze the correlation between commonly mutated genes and known genes associated with tooth agenesis in patients with non-syndromic tooth agenesis. The aim is to explore new genes that may be associated with tooth agenesis, to provide a genetic reference for its prevention as well as for the clinical diagnosis and treatment of tooth agenesis. METHODS: Genomic DNA was extracted from the peripheral blood of 18 congenitally edentulous subjects, and related gene mutations were identified by whole-exome sequencing. The genes related to maxillofacial development and the known pathogenic gene sequences of congenital tooth agenesis were selected for local alignment analysis of pairwise sequences, and the metric relationship of related sequences was determined. Hierarchical and fuzzy clustering methods were used for cluster analysis. RESULTS: Hierarchical clustering and fuzzy clusterings yielded consistent results. The EPB41L4A gene clustered with a large number of well-known and well-defined genes associated with tooth agenesis. From the perspective of cluster analysis, it can be inferred that the genes clustered together generally have similar functions. CONCLUSION: EPB41L4A, which is involved in the Wnt pathway, may be a candidate gene warranting further investigation.
Humans↗
[Cytology of chronic bronchitis: comparative study of sputum, brushing and bronchial biopsy (author's transl)].
Present research in chronic bronchitis is directed towards a technique of cytological detection. The object of the study was to compare the results of biopsy, brushing and sputum in 65 patients with clinical signs of chronic bronchitis. We established a good correlation between the results of biopsy and brushing. Unfortunately no reliable test for chronic bronchitis can be derived from the cytological study, which could indicate the future trend of the patient.
Biopsy↗
[Possibilities and limitations in the localisation of sources of infections (author's transl)].
In latent microbial hospitalism environmental checks of the patients, the staff and objects permit detection of pathogenic or potentially pathogenic bacteria which are silent epidemiologically. "One's own body" as a source of infection has not yet been sufficiently investigated in connection with auto-infections. Taking two staphylococcal epidemics as examples, reference is made to the monogenic and heterogenic forms of hospitalism as well as to the necessity of accurately identifying the strains for the purpose of localising the source of infection. In the monogenic form only one type of bacterial species is present (Table 2). In heterogenic hospitalism, infections are caused by various different species of bacteria or also by a single species. If this is the case, various biotypes, serotypes and phagotypes exist (Table 1). In addition, simple possibilities of biotyping the staphylococci are demonstrated. Defensive measures can be adopted selectively only when the epidemiology and the source of infection are known.
Anti-Bacterial Agents↗
Theoretical considerations of effects of x-ray film-screen characteristics on threshold detectability of small low-contrast objects.
An expression is derived for visual contrast of a radiographed small object that takes into account the proportion of scattered radiation as well as the shape of the sensitometric curve of the film-screen combination. Based on this expression for visual contrast applied to two different film-screen systems, predictions are made for threshold perceptability of low-contrast objects radiographed with the two imaging systems in the presence of scattered radiation and at different points on the sensitometric curves.
Absorptiometry, Photon↗
KRAS Mutations in Duodenal Lavage Fluid After Secretin Stimulation for Detection of Pancreatic Cancer.
OBJECTIVE: Although pancreatic ductal adenocarcinoma (PDAC) is still a devastating disease, the survival rate for surgically removed PDACs has significantly improved in recent years. Early detection is essential in managing PDAC. BACKGROUND: The presence of KRAS mutations in PDAC leads to the initial genetic abnormality and offers a significant timeframe for identifying resectable PDACs. A minimally invasive and highly specific PDAC screening test is necessary to prevent the need for invasive follow-up tests. METHODS: Between July 2021 and March 2023, 169 cases were enrolled in 7 institutions. By administering secretin before esophagogastroduodenoscopy (EGD), the excretion of pancreatic juice into the papillary fluid can be stimulated, creating a resource for testing. Washing fluid was collected using a specialized catheter from control individuals (n=75) and patients with resectable PDAC (n=89) at the initial diagnosis. A highly sensitive technique was employed to study KRAS gene mutations. RESULTS: This study obtained an AUC of 0.934 (95% CI: 0.904, 0.964) when using KRAS mutations in duodenal lavage fluid to differentiate between patients with resectable PDAC and healthy controls. The estimated sensitivities were calculated with specificity set at 100%, resulting in a sensitivity of 83.1% (95% CI: 71.7%, 91.2%). The McNemer test showed a significantly higher sensitivity for KRAS mutations than serum CEA and CA19-9 ( P <0.0001). CONCLUSIONS: We created a method to identify resectable PDACs by analyzing KRAS mutation levels in duodenal fluid collected during EGD with secretin stimulation of pancreatic juice secretion.
Humans↗
Relative detectability of intracranial calcifications on computed tomography and skull radiography.
Phantom studies comparing skull radiography and cranial computed tomography (CT) show that CT is 5 to 15 times more sensitive than skull radiography in the detection of intracranial calcifications. The difference in detectability varies with object size. The relative sensitivity of CT increases with increased object size within the constraints of the section thickness. The relationship of these findings to the detectability of pineal and choroid calcifications is discussed.
Brain↗
Splitting as a defense mechanism in children of alcoholic parents.
This paper is an attempt to identify an antecedent of overt symptomatology in children of alcoholic parents. Through art and verbal productions, within the context of a structured learning experience, primitive ego defenses and severely impaired object relations can be detected. The concept of splitting vs. repression in object relations theory and its implications for the future functioning of the child is discussed. Clinical material illustrating differences between the child who utilizes mature ego defenses and the child who resorts to splitting is presented.
Alcoholism↗
On-line statistical detection of average evoked potentials: application to evoked response audiometry (ERA).
An objective method of EP detection in averaged EEG epochs is described which is based on the statistical properties of the averaged EEG in the absence of time-locked EP activity. The statistical properties of the subject's averaged background EEG are summarized by making a set of comparisons between two consecutive EEG epochs immediately preceding each stimulus presentation. After every stimulus presentation, a second set of comparisons is made between the post-stimulus EEG epoch and the immediately preceding pre-stimulus EEG epoch. These two sets of comparisons are then examined to determine whether the latter differ significantly from the former. The technique has been programmed for on-line use on a 12-bit minicomputer, validated on cooperative adult subjects and used for ERA threshold determinations in young children. Using response detection criteria yielding an empirically determined false positive error rate of between 1 and 4%, threshold estimates averaged about 10 dB higher than psychophysical thresholds. The efficiency with which such ERA thresholds may be obtained with this method is substantially greater than that of conventional ERA procedures employing subjective evaluation of averages. Further, the technique allows variation of detection criteria to suit non-audiometric needs and empirical determination of the false-positive error rate under any set of conditions.
Adult↗
Detectable C-Peptide and Diabetic Ketoacidosis Risk in Type 1 Diabetes.
OBJECTIVE: To investigate whether detectable C-peptide levels in type 1 diabetes is associated with a lower risk of diabetic ketoacidosis (DKA). RESEARCH DESIGN AND METHODS: We analyzed the Diabetes Control and Complications Trial publicly available data repository for the association between detectable stimulated C-peptide (>0.2 nmol/mL, measured annually) and DKA incidence over an average 6.5-year follow-up. We used crude and adjusted Andersen-Gill models for recurrent DKA events with time-dependent covariates. RESULTS: Of the 1,441 participants (53% male, median age 27 years), 129 (9%) experienced 180 DKA events. Of these events, 179 (99.44%) occurred after C-peptide was ≤0.2 nmol/L in the prior year and only 1 event occurred with C-peptide >0.2 nmol/L. C-peptide >0.2 nmol/L was associated with a DKA hazard ratio of 0.07 (95% CI 0.01-0.48; P = 0.007), consistent across adjusted models. CONCLUSIONS: Endogenous insulin production was significantly associated with lower DKA risk, suggesting that treatments preserving insulin production could decrease long-term DKA risk.
C-Peptide↗
Optimizing prostate biopsy decision making - (MUSIC-screen): A 1:1 randomized controlled trial comparing micro-ultrasound versus multiparametric magnetic resonance imaging for prostate cancer diagnosis.
BACKGROUND: Micro-ultrasound (microUS) represents a potential alternative to multiparametric magnetic resonance (mpMRI) in guiding prostate biopsy, with level 1 evidence demonstrating non-inferiority to detect Grade Group ≥2 (GG ≥ 2) prostate cancer in biopsy-naïve men. However, a critical gap remains in the screening pathway, in which imaging is needed to identify men at risk and determine whether biopsy is warranted. METHODS: MUSIC-Screen is a phase 3, open-label, noninferiority 1:1 randomized controlled trial evaluating microUS as an alternative imaging compared to mpMRI for determining the need for prostate biopsy in biopsy- and imaging- naïve men at risk for GG ≥ 2. A total of 1284 men will be randomized to undergo either microUS or mpMRI. Men with PRI-MUS 3-5 or PI-RADS 3-5 lesion will undergo targeted and systematic biopsy. Men with negative imaging and PSA density ≥ 0.15 will undergo systematic biopsy, while those with PSA density < 0.15 will defer biopsy. RESULTS: The primary outcome is GG ≥ 2 detection in each study arm. The primary hypothesis is that microUS is non-inferior to mpMRI for screening and detection of GG ≥ 2. Secondary objectives include comparison of GG ≥ 2 detection rates in targeted cores among patients with PRI-MUS or PI-RADS scores of 3-5, proportion of men who defer biopsy but are diagnosed with GG ≥ 2 within 8 years, assessment of the negative predictive value of each imaging modality, and health economic analyses. CONCLUSION: MUSIC-Screen will determine whether microUS can be used as an imaging modality to inform biopsy decision making that is non-inferior to mpMRI for GG ≥ 2 prostate cancer detection. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT06626022.
Humans↗
Echolocation and pursuit of prey by bats.
Echolocating bats use different information-gathering strategies for hunting prey in open, uncluttered environments, in relatively open environments with some obstacles, and in densely cluttered environments. These situations differ in the extent to which individual targets such as flying insects can be detected as isolated objects or must be separated perceptually from backgrounds. Echolocating bats also differ in whether they use high-resolution, multidimensional images of targets or concentrate specifically on one particular target dimension, such as movement, to detect prey.
Animals↗