Characteristics of local blood flow in deep brain structures in epilepsy.
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Glycin, glutamic acid or casein hydrolysate induced high frequency and high voltage electrical waves in the brain-stem RF and often in the lateral hypothalamic nucleus and cortex. These changes were more obvious during feeding of the dogs. Sham feeding and parenteral administration of glucose or 0.9% solution of NaCl evoked responses different from the above ones. Gastric secretion stimulated by i.v. administration of nitrous substances by food was not directly related to the above responses either. The impulses responsible for the secretion seem to arise at another level of the brain.
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Acute and chronic experiments with recording of electrical activity of skeletal muscle, autonomic nerves, and some brain formations were performed on cats. Spectral correlation analysis showed that the spatial synchronization of electrical activity for the autonomic nerves and brain formations within the frequency range of 25--35 Hz, revealed by previous investigations, extends also to skeletal muscle. It is postulated that the presence of a widespread rhythm of 25--35 Hz is a factor facilitating the transmission of influences in the nervous system through frequency potentiation of synaptic action.
1. GABA was demonstrated in rat brain in fluorescence histochemistry. The alteration by application of submaximal doses of drugs of the distribution and intensity of the transmitter fluorescence was studied in hippocampus and cerebellum. 2. Isonicotinic acid hydrazide and Penicillamine leads to a distinct grain formation and diminishing of the fluorescence product, normally appearing homogenous. These effects are considered to reflect an enzyme (GAD) inhibition. 3. By Cycloserine a distinct increase of the GABA fluorescence is effected, presumably by inhibiting GABA-transaminase-activity. 4. Chloropromazine causes diminishing, grain formation and dislocation as well of the fluorescent product, supposedly due to an affection of the membrane binding properties of GABA. 5. Hypotheses concerning the chemical events of the GABA-reaction are worded.
The study deals with synaptic and spike responses of neurones in the rat sensorimotor cortex to stimulation of the lateral and medial hypothalamus, locus coeruleus and raphe nuclei. The activity of 57 neurones was recorded, 41 of them intracellularly and quasi-intracellularly, in response to the stimulation of sites in these structures, which were previously identified as "emotionally/ significant. No considerable differences in the effects of the stimulation of different "emotiogenic" zones were found. The stimulation parameters, differing from the "behavioural" ones by a greater strength, elicited in the majority of neurones clear post-synaptic responses, often in the form of EPSP-IPSP. Latencies of the responses varied from 3 to 80 msec. The most stable and pronounced responses were obtained to the stimulation of the lateral hypothalamus. No significant correlations of the latencies of the responses to the stimulation of different "emotiogenic" structures were found.
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Following a course-wise introduction of chlorpromazine, majeptil and trisedyl to rats the methionine-35S incorporation in the protein was diminished in most of the brain segments.