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The Old World and New World alphaviruses use different virus-specific proteins for induction of transcriptional shutoff.

Alphaviruses are widely distributed throughout the world. During the last few thousand years, the New World viruses, including Venezuelan equine encephalitis virus (VEEV) and eastern equine encephalitis virus (EEEV), evolved separately from those of the Old World, i.e., Sindbis virus (SINV) and Semliki Forest virus (SFV). Nevertheless, the results of our study indicate that both groups have developed the same characteristic: their replication efficiently interferes with cellular transcription and the cell response to virus replication. Transcriptional shutoff caused by at least two of the Old World alphaviruses, SINV and SFV, which belong to different serological complexes, depends on nsP2, but not on the capsid protein, functioning. Our data suggest that the New World alphaviruses VEEV and EEEV developed an alternative mechanism of transcription inhibition that is mainly determined by their capsid protein, but not by the nsP2. The ability of the VEEV capsid to inhibit cellular transcription appears to be controlled by the amino-terminal fragment of the protein, but not by its protease activity or by the positively charged RNA-binding domain. These data provide new insights into alphavirus evolution and present a plausible explanation for the particular recombination events that led to the formation of western equine encephalitis virus (WEEV) from SINV- and EEEV-like ancestors. The recombination allowed WEEV to acquire capsid protein functioning in transcription inhibition from EEEV-like virus. Identification of the new functions in the New World alphavirus-derived capsids opens an opportunity for developing new, safer alphavirus-based gene expression systems and designing new types of attenuated vaccine strains of VEEV and EEEV.

Alphavirus↗

Live high-train low associated with increased haemoglobin mass as preparation for the 2003 World Championships in two native European world class runners.

BACKGROUND: It is unclear whether world class endurance athletes, in contrast with less well trained subjects, increase their haemoglobin mass on a regimen of living high and training low (LHTL). OBJECTIVE: To assess whether haemoglobin mass increases in world class athletes on LHTL and whether this increase is associated with peak performance at a subsequent important competition. METHODS: Two Swiss world class runners (one 5000 m and one marathon) lived for 26 days (18 hours a day) at an altitude of 2456 m and trained at 1800 m. This LHTL camp was the preparation for the World Athletic Championships taking place 27-29 days after the end of the camp. Haemoglobin mass and other haematological variables were measured before and after the LHTL camp. The performance parameter was the race times during that period. RESULTS: Haemoglobin mass increased by 3.9% and 7.6%, and erythrocyte volume by 5.8% and 6.3%. The race times, as well as the ranking at the World Championships, indicated clearly improved performance after the LHTL camp. CONCLUSIONS: The results suggest that LHTL with an adequate dose of hypoxia can increase haemoglobin mass even in world class athletes, which may translate into improved performance at important competitions at sea level.

Adaptation, Physiological↗

Invasive amebiasis in naturally infected New World and Old World monkeys with and without clinical disease.

Histopathological preparations of cecum and colon from monkeys naturally infected with invasive Entamoeba histolytica were examined to determine the distribution of amebae in the tissues and the types of lesions, if any, associated with them. Infections were studied in 3 New World species (10 Callicebus moloch, 1 C. torquatus, and 2 Aotus trivirgatus) and 3 Old World species (8 Macaca mulatta, 6 Erythrocebus patas, and 1 Cercopithecus aethiops). Amebiasis was recorded as the principal or a contributing cause of death of all of the 13 New World monkeys and in 6 of the 15 Old World monkeys; amebiasis was detected in the rest of the monkeys only after tissues were re-examined specifically for amebae. Amebae causing no apparent damage were found in the lamina propriae, mainly at the muscularis mucosae. Most frequent were colonies or aggregates of amebae in the crypts between the epithelium and basement membrane, causing either no evident necrosis or changes ranging from necrosis and disarrangement of adjacent cells to complete destruction of the epithelium and reduction of the cells to pyknotic bodies. A lesion interpreted as possibly characteristic of carrier-state invasive amebiasis was destruction of the epithelium in patches of mucosal crypts, not leading to ulceration. Uncommon but present in both New and Old World monkeys were typical areas of surface erosion and classical flask-shaped ulcers. The observations show that in some species of Old World monkeys amebiasis can be invasive without causing clinical disease.

Amebiasis↗

Horizontal cell connections with short wavelength-sensitive cones in the retina: a comparison between New World and Old World primates.

Recent studies in the Old World macaque monkey have shown that the two horizontal cell types H1 and H2 differ with respect to their connections to short wavelength-sensitive (SWS) cones. We wanted to establish whether this pattern of connectivity is common to all primates. The connections of horizontal cells with SWS cones were studied in the retinas of two species of New World (marmoset and tamarin) and two species of Old World (orangutan and chimpanzee) primates by using a double-labelling technique. Horizontal cells were labelled with DiI and then photoconverted; SWS cones were labelled immunocytochemically. The marmoset shows a sex-linked polymorphism of colour vision: All males are dichromats, whereas most females are trichromats. In contrast, Old World primates are usually trichromats. Our results show that the horizontal cells of both New World and Old World primates have a comparable pattern of connectivity with SWS cones and thus indicate that the wiring of horizontal cells with SWS cones does not differ between dichromats and trichromats and is common to all primates. The H1 cells make no or only sparse contact with SWS cones. In marmoset, H1 cells have on average 0.8% of their dendritic terminals at SWS cones. The H2 cells contact all SWS cones in their dendritic field. In marmoset, H2 cells have on average 11.8% of their dendritic terminals at SWS cones. The axon of H2 cells contacts SWS cones but presumably also contacts other cones.

Animals↗

Plundering the poor: the role of the World Bank in the Third World.

The World Bank, the most important so-called development assistance agency, annually dispenses billions of dollars to Third World governments, ostensibly to "develop" their economics through a variety of loan projects. But even a superficial analysis reveals that the Bank is the perfect mechanism to help (i.e., subsidize) the large transnational corporations from the industrial countries to expand their industrial, commercial, and financial activities in the Third World, at the expense of the latter and particularly at the expense of the rural and urban proletariat. This article discusses Cheryl Payer's recent book, The World Bank: A Critical Analysis, in which she analyzes the Bank's role in the Third World and sets forth the major reasons why poverty, hunger, and malnutrition, as well as unemployment, and all the adverse social phenomena associated with them, are on the increase.

Developing Countries↗

Microsatellite analysis reveals substantial genetic differentiation between contemporary New World and Old World Holstein Friesian populations.

Genotypic data from 39 microsatellite loci typed in 211 animals were used to assess the genetic differentiation between Old World and New World Holstein Friesian cattle populations. Gene diversities were similar in all five Holstein Friesian populations surveyed, ranging from 0.43 to 0.48. A tree of individuals based on the proportion of shared alleles indicated a clear distinction between Old World and New World Holstein Friesian populations. Similarly, genetic differentiation between populations, as measured by FST, was highly significant. Using the split decomposition method, we were able to visualize the significant introgression of New World Holstein Friesian into European Holstein Friesian populations.

Alleles↗

Some demographic and economic aspects of Third World and First World urbanization and the economic implications thereof for South Africa.

"This paper analyses various demographic and economic features of the urbanization process that is taking place in Third World (developing) and First World (developed) countries. A comparison of some demographic and economic differences between contemporary Third World urbanization and First World urbanization at a comparable stage of development is made. The aim is to see what South African urbanization can learn from the comparison between Third and First World urbanization."

Africa↗

Retrovirus restriction by TRIM5alpha variants from Old World and New World primates.

The TRIM5alpha proteins of humans and some Old World monkeys have been shown to block infection of particular retroviruses following virus entry into the host cell. Infection of most New World monkey cells by the simian immunodeficiency virus of macaques (SIVmac) is restricted at a similar point. Here we examine the antiretroviral activity of TRIM5alpha orthologs from humans, apes, Old World monkeys, and New World monkeys. Chimpanzee and orangutan TRIM5alpha proteins functionally resembled human TRIM5alpha, potently restricting infection by N-tropic murine leukemia virus (N-MLV) and moderately restricting human immunodeficiency virus type 1 (HIV-1) infection. Notably, TRIM5alpha proteins from several New World monkey species restricted infection by SIVmac and the SIV of African green monkeys, SIVagm. Spider monkey TRIM5alpha, which has an expanded B30.2 domain v3 region due to a tandem triplication, potently blocked infection by a range of retroviruses, including SIVmac, SIVagm, HIV-1, and N-MLV. Tandem duplications in the TRIM5alpha B30.2 domain v1 region of African green monkeys are also associated with broader antiretroviral activity. Thus, variation in TRIM5alpha proteins among primate species accounts for the observed patterns of postentry restrictions in cells from these animals. The TRIM5alpha proteins of some monkey species exhibit dramatic lengthening of particular B30.2 variable regions and an expanded range of susceptible retroviruses.

Amino Acid Sequence↗

The inner world in the outer world: the phenomenology of posttraumatic stress disorder from a psychoanalytic perspective.

The intrapsychic processes underlying the phenomenology of PTSD symptoms appear to derive their fate from the states of consciousness at the time of traumatic experiences. The operative mechanisms of consciousness-condensation, avoidance of censorship, representability, and secondary revision-are the elements of trauma work as they are of dream work. These mechanisms establish an ever-present dynamic mental state of space consciousness, which is defined as an essential component of mental activities. This state of space consciousness exists beyond any experience of the self and beyond any processes regulated by the pleasure principle. When the sensory impressions derive from perceptions of and interactions with the mother, the matrix consists of the relationship between infant and mother. When the sensory impressions relate to traumatic circumstances the matrix reverts from the epigenetic context to the genetic one. The genetic matrix is manifest through disturbances in the activities of the higher mental functions. Under the impact of trauma, the epigenetic experiences are akin to a population floating without direction on a boat at the mercy of the elements-the raging higher mental functions. The factors operating on the higher mental functions create an inner world under traumatic circumstances, leading to an interpretation of the outer space according to the mental activities of the inner space. The concept of a dynamic process producing numerous modifications in mental scenes, thought, feelings, and reactions provides no surprise to psychoanalysts. The higher mental functions are seen as operating at the juncture of the physical and the mental. This juncture is the place where spatial consciousness and memories begin their common journey. Goodman wrote, "The conventional dichotomy between physical (organic) and mental (functional) is linguistic/conceptual rather than inherent in nature, and all events and processes involved in the etiology, pathogenesis, symptomatic manifestation, and treatment of psychiatric disorders are simultaneously biological and psychological" (Goodman, 1991, p. 553). The subjective experience of the dynamic unconscious activities of the higher mental functions form the inner nucleus selecting the relevant sensory impressions from the outer world. The juncture between the physical and the mental could be a crossroad for the psychic events occupying space and the psychic events determined by time factors. The psychic events that begin at this juncture find their composition evolving in time, in sequence, and in simultaneity. They follow a course determined by the four mechanisms responsible for the creation of dreams, of memories, of behaviors, and of narratives. The descriptive criteria of PTSD have in common the quality of persistence. The associated features show more variability and their appearance coincide with the appropriate circumstances that stimulate their arousal. Internal genetic factors remain persistent factors in the arousal of the reexperiencing phenomenon, of the avoidant behaviors and of the physiological reactivity. They furnish the impetus for the construction of the inner world, which is an indispensable tool in the management of ourselves, our bodies, and our environment. To paraphrase Freud's quotation used at the beginning of this article, there is a process in the inner world, stimulated by unconscious factors, that give rise to our view of the world and of our soul. The contents of this article-the ideas, the concepts, and the discourse itself-are a product of a continuing, evolving psychoanalytic way of thinking. The total of the analytic considerations gathered under the theory of trauma psychology could not be presented without the contributions of Freud, Hartmann, Rapaport, Erikson, Holt, Klein, Schafer, and others. We believe trauma psychology to be in its infancy, at a stage comparable to the stage of the libido theory at the time of the writing of The Interpretation

Adaptation, Psychological↗

Multiculturalism and pluralistic thought in nursing education: Native American world view and the nursing academic world view.

Nursing's capability to meet the health care needs of America's increasing racial diversity is dependent on its capacity to embrace multicultural groups entering the profession. Faculty should be aware of both the culture and world view of nursing's Euro-American (Anglo) culture-based curriculum. As an example of how world view and culture affect learning and performance, this article juxtaposes nursing's and the Native American's educational world view and culture. Each group's survival is dependent on the culturally determined and learned skills of its members. As faculty and students become culture brokers/interpreters between their world views, student success is enhanced. Acknowledgement of differing world views mandates that caution against generalizations, stereotyping, nefarious comparison, or the devaluing of persons be exercised. This knowledge, appropriately used, provides significant direction in guiding students and planning nursing educational approaches and programs.

Curriculum↗

The evolution of trichromatic color vision by opsin gene duplication in New World and Old World primates.

Trichromacy in all Old World primates is dependent on separate X-linked MW and LW opsin genes that are organized into a head-to-tail tandem array flanked on the upstream side by a locus control region (LCR). The 5' regions of these two genes show homology for only the first 236 bp, although within this region, the differences are conserved in humans, chimpanzees, and two species of cercopithecoid monkeys. In contrast, most New World primates have only a single polymorphic X-linked opsin gene; all males are dichromats and trichromacy is achieved only in those females that possess a different form of this gene on each X chromosome. By sequencing the upstream region of this gene in a New World monkey, the marmoset, we have been able to demonstrate the presence of an LCR in an equivalent position to that in Old World primates. Moreover, the marmoset sequence shows extensive homology from the coding region to the LCR with the upstream sequence of the human LW gene, a distance of >3 kb, whereas homology with the human MW gene is again limited to the first 236 bp, indicating that the divergent MW sequence identifies the site of insertion of the duplicated gene. This is further supported by the presence of an incomplete Alu element on the upstream side of this insertion point in the MW gene of both humans and a cercopithecoid monkey, with additional Alu elements present further upstream. Therefore, these Alu elements may have been involved in the initial gene duplication and may also be responsible for the high frequency of gene loss and gene duplication within the opsin gene array. Full trichromacy is present in one species of New World monkey, the howler monkey, in which separate MW and LW genes are again present. In contrast to the separate genes in humans, however, the upstream sequences of the two howler genes show homology with the marmoset for at least 600 bp, which is well beyond the point of divergence of the human MW and LW genes, and each sequence is associated with a different LCR, indicating that the duplication in the howler monkey involved the entire upstream region. [The sequence data described in this paper have been submitted to GenBank under accession nos. AF155218, AF156715, and AF156716.]

Animals↗

On the RNA world: evidence in favor of an early ribonucleopeptide world.

A highly complex RNA world, as is sometimes presented in view of the widespread and diversified use of RNA enzymes, would have encountered many difficulties in passing to a world with catalysis mediated by proteins. These difficulties can be overcome by postulating a very early relationship between the nucleotide and the amino acid components. In particular, after asserting that some characteristics expressed by (nucleotide) coenzymes in catalysis are easier to understand if a close and early relationship between these coenzymes and amino acids is hypothesized, a model is presented for the origin of the enzyme-coenzyme complex. This model is essentially based on an intermediate formed by a tRNA-like molecule covalently linked to a polypeptide. The model attributes the majority of the catalytic role in the ribonucleoprotein world to the latter complex and thus it takes into account the birth of the key intermediate in the origin of protein synthesis-namely, peptidyl-tRNA, which would have otherwise been extremely difficult to select. The predictions of the model are discussed along with its robustness, using the data derived from the study of intermediary metabolism and those from molecular biology. Finally, the appearance of the genetic code in the late phase of the ribonucleopeptide world is discussed.

Animals↗

Enucleation demonstrates ocular dominance columns in Old World macaque but not in New World squirrel monkey visual cortex.

The effect of monocular enucleation on basophilic and metabolic staining in primary (striate) visual cortex has been compared in Old and New World monkeys. Both species show a 30-40% shrinkage of neurons in the layers of dorsal lateral geniculate nucleus receiving axons from the enucleated eye. In striate cortex Old World macaque monkeys show alternating bands of increased and diminished staining in layers 3, 4 and 6, corresponding to ocular dominance columns. New World squirrel monkeys show staining patterns in all layers which are unchanged from normal cortex, suggesting that New World monkeys lack obvious ocular dominance columns.

Animals↗

New world, but not Old World, monkeys carry several genes encoding beta-microseminoprotein.

It was shown by Southern hybridization that cotton-top tamarin and common marmoset, New World monkeys, carry three or more genes encoding beta-microseminoprotein, also known as PSP94. In contrast, the genomes of Old World monkeys, as represented by rhesus macaque and sacred baboon, contain a single gene. Clones containing three different genes encoding beta-microseminoprotein were isolated from a cotton-top tamarin genomic library. They carry two complete genes of four exons and a third gene lacking the first exon. The structure suggests that the three genes are functionally active and give rise to transcripts that are approximately 86% similar in sequence. By sequencing one gene in full, it was shown that the introns carry an excess of interspersed repeats, on average 29% of the introns consist of Alu repeats. A phylogenetic analysis demonstrated that the genes probably arose in New World monkeys after the separation from Old World primates.

Alu Elements↗

Endogenous New World primate retrovirus: interspecies antigenic determinants shared with the major structural protein of type-D RNA viruses of Old World monkeys.

A reverse transcriptase-containing virus has recently been isolated from a squirrel monkey (Saimiri sciureus). Molecular hybridization studies demonstrate that the squirrel monkey retrovirus (SMRV) is endogenous to this New World primate, yet lacks detectable nucleotide sequence homology with cellular DNAs of representative Old World primates or with the genomes of previously isolated Old World primate retroviruses. The 35,000-dalton major structural protein (p35) of SMRV was purified and shown to possess antigenic determinants distinct from those of known retroviruses. While SMRV was found to lack antigenic determinants broadly shared among mammalian type-C viruses, immunologic crossreactivity was demonstrated between SMRV p35 and the major structural protein (p26) of Mason-Pfizer monkey virus, a prototype type-D retrovirus of Old World monkeys. These findings support the concept that SMRV and Mason-Pfizer monkey virus are evolutionarily related, and raise the possibility that a progenitor of type-D retroviruses became genetically associated with primates at a very early time in their evolution.

Animals↗

Quality of care and the burden of two cultures: when the world of the nurse's aide enters the world of the nursing home.

Using ethnographic data, the two worlds of the nurse's aides were examined, the world in which they live and the world in which they work. Because the institutional culture of the nursing home often adds to the adversity of their personal life, the nurse's aides move between the two milieus in a self-perpetuating negative cycle. Aides carry to the bedside of elderly residents the affective and psychological burden these two worlds place on them.

Adult↗

Corchorus yellow vein virus, a New World geminivirus from the Old World.

A bipartite begomovirus infecting Jute mallow (Corchorus capsularis, Tilliaceae) in Vietnam was identified using novel degenerate PCR primers. Analysis of this virus, which was named Corchorus yellow vein virus (CoYVV), showed that it was more similar to New World begomoviruses than to viruses from the Old World. This was based on the absence of an AV2 open reading frame, the presence of an N-terminal PWRLMAGT motif in the coat protein and phylogenetic analysis of the DNA A and DNA B nucleotide and deduced amino acid sequences. Evidence is provided that CoYVV is probably indigenous to the region and may be the remnant of a previous population of New World begomoviruses in the Old World.

Americas↗

Polypeptide toxins from the venoms of Old World and New World scorpions preferentially block different potassium channels.

Venoms from five Old World and two New World scorpions were tested for their ability to block various K+ channels in rat brain synaptosomes. A 86Rb efflux kinetic assay was used to identify three types of K+ channels, Ca(2+)-independent, voltage-gated, inactivating (A-type) and noninactivating (delayed rectifier) K+ channels and Ca(2+)-activated K+ channels [J. Physiol. (Lond.) 361:419-440, 441-457 (1985)]. The venoms from the Old World scorpions all blocked the A-type K+ channel but not the delayed rectifier K+ channel; only venom from the Israeli scorpion, Leiurus quinqestriatus hebraeus (Lqh), blocked the Ca(2+)-activated K+ channel. In contrast, venoms from the two New World scorpions selectively blocked the delayed rectifier K+ channel. Water-soluble components from Lqh venom from the Brazillian scorpion, Tityus serrulatus (Ts), were separated by ion exchange high performance liquid chromatography (HPLC). Seven components that blocked synaptosome K+ channels were isolated from Lqh venom by ion exchange HPLC. All seven components blocked the A-type K+ channel; the five most potent toxins had IC50 values of 18-40 nM. Two of the components from Lqh venom (one identified as charybdotoxin and the other denoted as Lqk4) also blocked a Ca(2+)-activated K+ channel (IC50 = 15 and 60 nM for charybdotoxin and Lqk4, respectively). Five K+ channel-blocking components were isolated from the Ts venom; all five blocked the delayed rectifier channel selectively, and the two most potent components had IC50 values of 8 and 30 nM. Several of the more potent Lqh and Ts toxins were purified to near-homogeneity by reverse phase HPLC. These toxins should be useful as ligands for K+ channel purification, for elucidation of K+ channel structure, and for studies of K+ channel function.

Animals↗