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[Contribution to the study of Microphallidae travassos, 1920 (Trematoda). XLV. Description of Maritrema feliui n. sp., parasite of shrews (Mammals) in Spain].

The authors describe and illustrate the morphology of the adult stage of Maritrema feliui n. sp. (Trematoda Microphallidae) obtained from the intestine of the shrew Crocidura russula (Hermann, 1780), definitive host of the fluke present in the estuary of the river Llobregat (Catalonia, Spain). The larval stages are found in two hosts obtained from the area: 1) Mercuria confusa (Frauenfeld) [= Pseudamnicola similis (Draparnaud)] (Mollusca: Hydrobiidae), and 2) Gammarus aequicauda (Martynov) (Crustacea: Amphipoda). The adult stage is anatomically related to the Maritrema with a mean size of the body, two equal suckers, a short oesophagus and long caeca, and a short and transversal cirrus pouch with a smooth and filiform cirrus. According to morphological and geographical affinities, the authors propose the synonymies of 1) Maritrema "opisthometra" sensu Bridgman et al., 1972 with M. chiriacae Deblock, 1975; 2) Maritrema longiforme Kifune et al., 1972, with Maritrema laricola Ching, 1963. Besides Maritreminoides congjiangensis Chiu et Gu, 1981 is transferred into the genus Quasimaritrema Deblock, 1973 according to Maritreminoides Rankin is invalidated.

Animals↗

Human parasitism by Phagicola sp (Trematoda, Heterophyidae) in Cananéia, São Paulo state, Brazil.

We report one case of parasitism by Phagicola sp. (Trematoda, Heterophyidae) in a 31 year-old woman who, in 1987, travelled and stayed several months in the municipality of Cananéia (SP), where she ingested, in various occasions, raw mullet (Mugil sp.). The patient referred mild intestinal pain and laboratory examinations showed eggs of Phagicola sp. in the stools and a slight increase in eosinophil blood levels (8%). After treatment with praziquantel (75 mg/kg per day for three days) all the symptoms and signs disappeared. This is, certainly, the first record of human infection by Phagicola sp. in Brazil and, perhaps, in countries other than the U.S.A. where unclear references to a few human cases were reported in the South-eastern region.

Adult↗

Redescription of Crepidostomum opeongoensis Caira, 1985 (Trematoda: Allocreadiidae) from fish hosts Hiodon alosoides and Hiodon tergisus (Osteichthyes: Hiodontidae).

Crepidostomum opeongoensis Caira, 1985 (Trematoda: Allocreadiidae) is redescribed from fish hosts Hiodon alosoides and Hiodon tergisus in southern Manitoba. The redescription adds details regarding the surface morphology and reproductive structures of the parasite not described previously. These include the characteristic tegumental papillae around the oral opening, paired papillae on the ventral and dorsal surfaces of the body, and tegumental bosses on the dorsal surface of the forebody. Crepidostomum opeongoensis co-occurred with its hypothesized sister species. Crepidostomum illinoiense, in all host individuals harboring the former, and both species are characteristic of hiodontids. The association of C. opeongoensis with hiodontids and the absence of hiodontids in Lake Opeongo where C. opeongoensis was originally reported indicate that the Algonquin Park region may have had hiodontids in the past and that the life cycle may be completed at present without the original fish host.

Animals↗

Glypthelmins poncedeleoni n. sp. (Trematoda: Macroderoididae) of amphibians from the neotropical region of México.

A new species of Glypthelmins (Trematoda: Macroderoididae) is described from the intestine of Bufo marinus and Leptodactylus melanonotus from several localities of the Neotropical Region of Mexico. Glypthelmins poncedeleoni n. sp. can be differentiated from other species of the genus by having extracecally distributed vitellaria, ovary always larger than testes, and vitelline follicles grouped in 7 post-testicular, nonoverlapping, rosette-like clusters and 5 pretesticular overlapping clusters.

Animals↗

Life cycle of Brachylaima mascomai n. sp. (Trematoda: Brachylaimidae), a parasite of rats in the Llobregat delta (Spain).

The terrestrial triheteroxenous life cycle of Brachylaima mascomai n. sp. (Trematoda: Brachylaimidae) is elucidated. Operculated, assymetric, embryonated eggs (25.4 x 12.7 microm) are passed with feces of the natural (Rattus norvegicus and R. rattus) and experimental (albino and wild mice, albino rats, Apodemus sylvaticus, Mus spretus [Muridae] and the golden gerbil) definitive hosts and ingested by the helicid gastropod Pseudotachea splendida, the only natural and experimental first intermediate host. Microcaudate cercariae harbored in branched sporocysts in the digestive gland emerge from this snail and contact P. splendida, Otala punctata, Theba pisana, and Helix (C.) aspersa snails developing into unencysted infective metacercariae in the kidney. Definitive hosts are infected by ingestion of infected snails; the adult parasites inhabit the small intestine. Chaetotaxic cercarial pattern specific at acetabular (S(II) 8-10 papillae) and cephalic (C(III) 13-15 papillae, H 16 papillae) levels. Three types of cercarial papillae are observed by scanning electron microscopy (SEM) and their arrangement is correlated with chaetotaxy for the first time in trematodes: argentophilic papillae with fingerlike process (cephalic, body, and acetabular levels), argentophilic papillae with opening (2 papillae in the M body level), and nonargentophilic dome-shaped papillae (alternated with argentophilic S(II) papillae on the ventral sucker). SEM detected interlacing network of ridges covering the metacercarial body. Adults with multidigitate tegumentary spines were observed by SEM. Subequal suckers; the acetabulum located in the posterior part of anterior fifth of body. Vitellaria extend from between middle level and anterior margin of anterior testis to between middle level and posterior margin of acetabulum. Uterus almost reaches the intestinal bifurcation.

Animals↗

Bakkeius moragai n. gen. et n. sp. and Pojmanskia riosae n. gen. et n. sp. (Trematoda: Digenea: Brachylaimoidea) in birds from the Area de Conservación Guanacaste, Costa Rica.

We describe 2 new species of leucochloridiid-like brachylaimoid digeneans parasitizing a variety of birds in the Area de Conservación Guanacaste, Costa Rica, each of which we assign to a new genus. According to Pojmanska's (Pojmanska, T. 2002a. Superfamily Brachylaimoidea Joyeux & Foley, 1930. In Keys to the Trematoda, D. I. Gibson, A. Jones, and R. A. Bray [eds.]. CAB International and The Natural History Museum, London, U.K., p. 31-36.) key for the Brachylaimoidea, we are unable to place either species in any family. One species most closely resembles members of Leucochloridium by having well-developed suckers, lacking an esophagus, and having cecal shoulders, gonads at the posterior end, and the genital pore at posterior end of body but differs by having symmetrical testes, a posttesticular ovary, and a terminal genital pore; thus, we propose the genus Bakkeius for it. The second new genus resembles members of Michajlovia by having ventral genital pores but differs by having extracecal uterine loops in the forebody, a cirrus sac containing the pars prostatica and seminal vesicle, and gland cells surrounding the genital pore; thus, we propose Pojmanskia for it. These new genera must currently be treated as incertae sedis according to Pojmanska (op. cit.); however, we feel that future phylogenetic analyses will require emendation of the family diagnosis for Leucochloridiidae to include those taxa with terminal and ventral genital pores and with preovarian testes.

Animals↗

Neoplagioporus zacconis (Trematoda: Opecoelidae) from the intestine of the pale chub, Zacco platypus, in Korea.

Neoplagioporus zacconis (Yamaguti, 1934) (Trematoda: Opecoelidae) was found from the intestine of the pale chub, Zacco platypus, for the first time in Korea. The worms were characterized morphologically by oval body shape, bipartited seminal vesicle, sinistrally located genital pore, and lobed ovary, and distinguished from the two other species of Neoplagioporous by body shape and distribution of vitelline follicles. The morphological characteristics, except the ovary, well corresponded to those of the previous descriptions. The morphological difference of the ovary was considered as a character of geographical phenotypic variation.

Animals↗

Surface ultrastructure of the adult stage of Acanthotrema felis (Trematoda: Heterophyidae).

The surface ultrastructure of Acanthotrema felis (Trematoda: Heterophyidae) adults, recovered from a kitten experimentally infected with the metacercariae, was observed using a scanning electron microscope. The worm was leaf-like, ventrally concave and covered with scale-like multi-pointed tegumental spines. The spines on the anterior surface were short but broad, and had 10-12 pointed tips. The cytoplasmic processes protruded around the spines, like pockets for the spines. The ventrogenital opening was crescent, or kidney-shaped, and had protuberances with minute spines on its surrounding tegument. The spines on the posterior surface were long, but narrow, with 6-8 pointed tips. The cytoplasmic processes on this tegument were ridge-like, and elevated along the row of the spines. The surface ultrastructure of A. felis is generally similar to that of other heterophyid flukes, but some features are characteristic, and may be of taxonomic and bio-ecological significance.

Animals↗

Bothrigaster variolaris (Trematoda: Cyclocoelidae) infection in two Florida snail kites (Rostrhamus sociabilis plumbeus).

Two Florida snail kites (Rostrhamus sociabilis plumbeus, Say) collected in August and December 1993 from Osceola County (28 degrees 15'N, 81 degrees 17'W) and Glades County (26 degrees 45'N, 81 degrees 10'W), Florida (USA), respectively, were infected with Bothrigaster variolaris (Trematoda: Cyclocoelidae); this parasite has not been reported previously from birds in the United States. One bird, a fledgling female, harbored 270 specimens of B. variolaris; the helminths caused airsacculitis and bronchitis, possible contributing factors in the death of this bird. The other bird, a subadult female, was infected with 40 trematodes and had gross and microscopic lesions consistent with trauma as the cause of death.

Air Sacs↗

Prouterina wescotti n. gen., n. sp. (Trematoda: Prouterinidae N. Fam.) from the brain, lungs, and nasal sinuses of a black bear (Ursus americanus) from Idaho.

Prouterina wescotti gen. n. and sp. n. (Trematoda: Prouterinidae N. Fam.) is described from a free-ranging black bear (Ursus americanus) which died in May 1995 in northern Idaho (USA). Adult digenetic trematodes were detected in brain, lungs, and nasal sinuses, and were likely responsible for the emaciated condition, copious nasal discharge, neurological signs, and death of the bear. Mature trematodes recovered from the bear were conical with small spines on the tegument. The anterior end was broad and tapered gradually toward the posterior. Mean (+/-SE) size of the mature trematodes was 3.67 (+/-0.08) by 2.14 (+/-0.04) mm (n = 80). Eggs are operculated, gold, and 68.2 (+/-0.42) by 41.4 (+/-0.41) microns (n = 75). Suckers are well developed and located in the anterior half of the body, with the genital pore just posterior to the ventral sucker. Testes are tandem and the ovary is lateral and slightly anterior to the anterior testes just posterior to the ventral sucker. The uterus is predominantly anterior to the ventral sucker and is the most distinctive feature of the trematode.

Animals↗

Accumulation and excretion of neutral lipids in the metacercaria of Leucochloridiomorpha constantiae (Trematoda) maintained in vitro.

Histochemical and thin-layer chromatographic analyses were made on neutral lipids in Leucochloridiomorpha constantiae (Trematoda) metacercariae incubated in non-nutrient Locke's solution at 37.5 or 42 C for 24 hr. As determined by Oil Red O staining, lipid droplets accumulate mainly in the intestine of incubated worms. Attempts to distinguish specific neutral lipid fraction of metacercariae obtained directly from snails is free sterol. During incubation, metacercariae accumulate triglycerides and to a lesser extent cholesterol esters, and excrete free sterol into the medium.

Animals↗

Unitubulotestis sardae (Trematoda: Didymozoidae) infection in Atlantic bonito Sarda sarda (Perciformes: Scomberomoridae) in the Ionian and Tyrrhenian Seas: histopathological and SEM investigations.

Nematobothrinae are trematoda digenea that usually live in cysts in fish tissues. They have been reported in different teleost species although the host-parasite relationships remain to be elucidated. This report is aimed at analysing the presence of Unitubulotestis sardae in the Atlantic bonito (Sarda sarda) populations fished in the Straits of Messina. The histopathological change reported in literature in similar cases is an aneurisma verminosa saccata, whereas, in the specimens here described, we show neovascularisation and at the same time, hyperplasia of the gill epithelium, leading to the formation of a bistratified cystic wall. The SEM examination of the parasitic cysts gave us a better understanding of the surface microanatomical details of the cystic walls. The external surface appeared to be constituted by a pluristratified covering epithelium of the gill arch. The internal layer was constituted by a net of blood vessels richly anastomosed with those originating from the gill arch arteries. This could be in agreement with data reported by Grabda (1947) on the localisation of the juvenile stages of the parasite in this blood vessel, but the evolution in cysts formation does not show similarities with the formation of an aneurisma.

Animals↗

[New metacercariae of the genus Tylodelphys (Trematoda, Diplostomatidae) in lacustrine populations of Galaxias maculatus (Teleostei, Galaxiidae)].

Metacercariae of three new species of Tylodelphys (Trematoda, Diplostomatidae) were found in the brain of Galaxias maculatus (Teleostei, Galaxiidae), at the Meliquina Lake, Neuquen Province, Argentina. These metacercariae are morphologically described as Tylodelphys argentinus n. sp., Tylodelphys barilochensis n. sp. and Tylodelphys crubensis n. sp. This is the first time that a stage of genus Tylodelphys is mentioned parasitizing Galaxias maculatus. Metacercariae of T. barilochen sis n. sp. were also found in G. maculatus brain at the Perito Moreno Lake, Rio Negro Province, Argentina.

Animals↗

Carettacola hawaiiensis n. sp. (Trematoda: Spirorchidae) from the green turtle, Chelonia mydas, in Hawaii.

Carettacola hawaiiensis n. sp. (Trematoda: Spirorchidae) is described from the hepatic vessels of the green turtle, Chelonia mydas (L.), in Hawaii. The new species differs from any previously described species of Carettacola in size, placement of vitellaria, and shape and placement of Laurer's canal. The genus Haemoxenicon Martin and Bamberger, 1952, becomes a synonym of Carettacola Manter and Larson, 1950. Haemoxenicon stunkardi Martin and Bamberger, 1952, is transferred to the genus Carettacola Manter and Larson, 1950, and becomes Carettacola stunkardi n. comb. An emended generic diagnosis for Carettacola is given along with a key to the species.

Animals↗

In vivo and in vitro excystation of Zygocotyle lunata (Trematoda) metacercariae and histochemical observations on the cyst.

Encysted metacercariae of Zygocotyle lunata (Trematoda) excyst within 2 hr postexposure in the lower ileum of the domestic chick. Optimal in vitro excystation of this species occurs following pretreatment of the cyst for 15 min in 1% acidified pepsin, treatment in 0.02 M sodium dithionite (a reductant) for 1 to 2 min and then 2 hr treatment in an excystation medium containing 1% sodium glycocholate plus 1% trypsin in Earle's BSS adjusted to pH 8.8 with tris and maintained at 41 C. The cyst of this species is a dome-shaped hemisphere containing an inner and outer wall. The outer wall contains mainly acid mucopolysaccharides, whereas the inner wall is mainly proteinaceous. The cyst contains a ventral lid which only was visualized during excystation.

Animals↗

Hemolytic and coagulation properties of Sphaeridiotrema globulus (Trematoda).

Metacercariae of Sphaeridiotrema globulus (Trematoda) were obtained from naturally infected Goniobasis virginica (Pleuroceridae). Excysted metacercariae were placed individually on blood-agar plates. After 8 hr of incubation at 42 C the plates exhibited beta-hemolysis. The reaction zone was approximately 1.5 mm in diameter. Laboratory-reared mallard ducks (Anas platyrhynchos) were infected with S. globulus. Mallards developed fatal sphaeridiotremiasis and demonstrated increased prothrombin time on days 3 and 6 postinfection.

Animals↗

[Contribution to the study of Microphallidae Travassos 1920 (trematoda). XXXII. Microphallus breviatus n. sp., a species with an abbreviated evolutive cycle from a Mediterranean pond in the Languedoc].

Contribution to the study of Microphallidae Travassos, 1920 (Trematoda). XXXII. - Microphallus breviatus n. sp., a short life-cycle species of a mediterranean pond of Languedoc. The whole larval life-cycle of M. breviatus takes place in one host, Hydrobia ventrosa (Montagu), Mollusc Hydrobiidae. Hepato-pancreatic sporocysts produce morphologically altered xiphidio-cercariae which become encysted metacercariae in the sporocyts themselves. This species is defined in the genus by the anatomic characteristics of its cercariae and metacercariae, allied with its uncommon biology including two hosts only.

France↗