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Results for “Skin Window Technique”

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PubMed1978

Cellular reaction to autologous tumor tissue followed by "skin window" technique in orofacial tumors.

In a sample of patients with pseudotumors and benign and malignant tumors of the orofacial region there has repeatedly been investigated, after radical surgery, the cell reaction to autologous tumor tissue applied at indicated time intervals on a small skin lesion. The prognostically favorable picture of a cellular-type hypersensitivity reaction with massive participation of lymphoid cells and basophil granulocytes was only found in some basaliomas and leukoplakias. Conversely, spinocellular carcinomas are, irrespective of localization, characterized by areactivity.

Adult↗
PubMed2004

Microvascular response in guinea pig skin to histamine challenge with and without application of skin window.

We measured the microvascular response to histamine in guinea pig skin. Histamine (40 mg ml(-1)) was given either as a skin prick test or applied topically onto a skin window. The skin window was prepared by applying suction and gentle warming to the skin so that a blister was formed, and by removing the top of the blister. The microvascular response was measured as the accumulation of radiolabelled transferrin in the skin in vivo, reflecting a combination plasma exudation and vasodilatation. In the control (saline) challenge, the response was slightly greater in the skin window than after skin prick challenge and the scatter was larger. Histamine challenge resulted in a significant microvascular response with respect to the control situation when measured immediately after provocation for both challenge techniques. Ten minutes after challenge, a smaller response was measured, which was still significantly greater than control for the skin prick challenge, but not for topical provocation using the skin window technique. We conclude that the microvascular response to histamine after provocation with the skin prick technique is similar to that after topical provocation using the skin window technique. The skin window technique may have a lower sensitivity than the skin prick technique owing to a higher scatter in the control situation. This difference should be considered when performing and interpreting studies of the microvascular reaction in the skin.

Administration, Topical↗
PubMed1993

Skin eosinophilia in patients with allergic asthma, patients with nonallergic asthma, and healthy controls. II: 20-Hydroxy-leukotriene B4 is a potent in vivo and in vitro eosinophil chemotactic factor in nonallergic asthma.

BACKGROUND: In allergic and nonallergic asthma, eosinophils play an important effector role. However, because the pathogenesis of these types of asthma seems different, the mechanisms responsible for the tissue mobilization of those cells may be different. The in vivo and in vitro migratory response of eosinophils from patients with allergic and nonallergic asthma toward 20-hydroxy-leukotriene B4 (20-OH-LTB4), which is reported here, illustrates this. METHODS: By means of the Rebuck skin window technique the in vivo skin mobilizing capacity of intracutaneously applied buffer, LTB4, and 20-hydroxy (OH)-LTB4 was evaluated in healthy subjects (n = 6), subjects with allergic asthma (n = 14), and subjects with nonallergic asthma (n = 17). Also the in vitro chemotactic responsiveness of eosinophils from the circulation of both patient groups (both n = 8) toward buffer, LTB4, and 20-OH-LTB4 were tested by use of a microchemotaxis chamber technique. RESULTS: Although none of the substances were capable of inducing macroscopic observable skin reactions, intracutaneously applied 20-OH-LTB4 had an almost similar capacity to mobilize eosinophils in the skin of the subjects with nonallergic asthma as allergens had in subjects with allergic asthma. In 92% of the tested subjects with nonallergic asthma significant skin eosinophilia was observed. By contrast, LTB4 did not induce significant skin eosinophilia in both patient groups compared with buffer solution. This in vivo eosinophil mobilizing capacity of 20-OH-LTB4 in subjects with nonallergic asthma was confirmed by in vitro chemotaxis studies. Dose ranges of both LTB4 and 20-OH-LTB4 proved to be potent chemoattractants for eosinophils from patients with nonallergic asthma, but not for those of healthy subjects and those with allergic asthma. CONCLUSIONS: Our results indicate that 20-OH-LTB4 may be involved in the tissue mobilization of eosinophils in nonallergic asthma and that in vitro 20-OH-LTB4 (and LTB4) may act as potent chemotactic factors on eosinophils from those patients.

Adult↗
PubMed1981

Suction skin blister, skin window, and skin chamber techniques to determine extravascular passage of cefotaxime in humans.

We report the results obtained in comparative study on the extravascular passage of cefotaxime, employing three different methods: suction skin blister, skin window, and skin chamber. Applying the skin blister method in two different ways, we also studied the influence that suction pressure and time lapse between blister formation and antibiotic injection had on the results obtained in order to standardize the method and establish repeatability of the results. Using the skin chamber method, we studied the influence that the different protein contents in the fluid used to fill the skin chamber had on extravascular concentrations.

Adolescent↗
PubMed1991

Therapeutic effects of cetirizine in delayed pressure urticaria: clinicopathologic findings.

Cetirizine, a peripheral H1 antagonist, was administered to patients with delayed pressure urticaria in a double-blind, placebo-controlled, crossover study. Efficacy in reducing pressure-induced wheals and flares was evaluated. Histologic changes were also assessed with the skin window technique in weight-induced wheals. Results showed a statistically significant reduction in weight-induced wheal area (p less than 0.01) after cetirizine therapy; this improvement was accompanied by a concomitant reduction in eosinophil recruitment as demonstrated by the skin window technique (p = 0.0029). Subsequently, 14 patients with delayed pressure urticaria underwent biopsy before and after 3 weeks of cetirizine therapy to evaluate the drug's histologic effects. A blinded observer performed the histologic studies. Weight-induced lesions showed a mixed inflammatory infiltrate, primarily polymorphonuclear (neutrophils and eosinophils), whereas the unchallenged skin sites were normal. Cell counts from pressure-induced lesions showed a significant reduction in eosinophils after cetirizine treatment.

Adult↗
PubMed1985

Transcapillary diffusion of Na-fluorescein measured by a 'large window technique' in skin areas of the forefoot.

A method is introduced for the study of transcapillary diffusion of 20% Na-fluorescein (0.3 ml/l of blood given intravenously) in skin areas by a fluorescence video-microscopy system which has been used earlier for measurements at the single capillary level or on axes crossing capillary groups. Two groups of 17 and 14 healthy volunteers respectively were included in a test-retest experiment (first measurement at day one, second measurement at day three). The appearance of the dye was visualized and the intensity of fluorescent light measured by a quadratic video densitometer window at the forefoot in an area of 2 mm2 containing 78 capillaries on the average. In both groups with and without control of the skin temperature, there were no statistically significant differences of the mean values measured at the first and second day of study. However, the mean intraindividual differences and the coefficients of variance were significantly (p less than 0.005) higher in the subjects without temperature control. It is concluded that the method is suited to detect transcapillary diffusion of Nafluorescein in skin areas by an almost atraumatic procedure with acceptable reproducibility. Potentially, it may be used to follow the natural history of microvascular diseases and to test the effect of various treatment modalities.

Adult↗