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At least 37 records · Page 2Linked to original sources
PubMed1980

Oligonucleotide sequence analyses indicate that vesicular stomatitis virus large defective interfering virus particle RNA is made by internal deletion: evidence for similar transcription polyadenylation signals for the synthesis of all vesicular stomatitis virus mRNA species.

RNase T(1) oligonucleotide fingerprint analyses of three vesicular stomatitis virus Indiana serotype small defective interfering (DI) particle RNA species indicate that they only have oligonucleotides derived from the 5' region of the viral genome. These studies also indicate that these three DI RNAs have partial L gene sequences as well as two 5' viral oligonucleotides (59 and 70) that are not transcribed into L (or other) mRNA species (J. P. Clewley and D. H. L. Bishop, J. Virol. 30:116-123, 1979). Analyses of the large DI RNA (LT DI) reveal a different origin. The LT DI RNA has oligonucleotides derived from both the 3' end of the genome (including all the large oligonucleotides identified for N, NS, M, and G genes), in addition to at least one of the 5'-proximal L gene oligonucleotides (47), as well as all seven oligonucleotides (3, 38, 42, 43, 44B, 59, and 70) that are not protected from nuclease digestion after the formation of mRNA-viral RNA duplexes (Clewley and Bishop). It appears therefore that the genesis of LT RNA involves a deletion of internal L gene sequences from the viral RNA. Oligonucleotide sequence analyses have been undertaken on several of the vesicular stomatitis viral RNA oligonucleotides, including all seven (3, 38, 42, 43, 44B, 59, and 70) that are not transcribed into mRNA. The analyses confirm that oligonucleotides 59 [3'...GAACACCAAAAAUAAAAAAUA(G)...5'] and 70 [3'...GACCAAAACACCA(G)...5'] are at the 5'-end region of the viral genome. Oligonucleotide 38 [3'...GAAAUUCAUACUUUUUU(U)(G)...5'] may represent the termination signal for L mRNA synthesis (R. A. Lazzarini, personal communication). Oligonucleotide 43 [3'...GUAUACUUUUUUU(G)...5'] corresponds to the sequence shown to be the N gene mRNA polyadenylation signal (D. J. McGeoch, Cell 17:673-681, 1979). The other three oligonucleotides share a common feature with oligonucleotides 43 and 38, viz., a stretch of 6 or 7 U residues preceded by an AUAC sequence. Thus the sequence of oligonucleotide 3 is 3'...GAAUUAAUAUAAAAUUAAAAAUUAAAAAUACUUUUUU(U)(G)...5', whereas that of oligonucleotide 42 is 3'...GAUACUUUUUUUCAU(U)(G)...5', and that of oligonucleotide 44B is 3'...G(U)AUACUUUUUU(G)...5'. These sequence analyses suggest a common polyadenylation signal for the synthesis of all vesicular stomatitis virus mRNA species, i.e., the sequence (3')...AUACUUUUUU(U)...(5').

Animals↗
PubMed2001

Stomatal density and stomatal index as indicators of paleoatmospheric CO(2) concentration.

A growing number of studies use the plant species-specific inverse relationship between atmospheric CO(2) concentration and stomatal density (SD) or stomatal index (SI) as a proxy for paleo-CO(2) levels. A total of 285 previously published SD and 145 SI responses to variable CO(2) concentrations from a pool of 176 C(3) plant species are analyzed here to test the reliability of this method. The percentage of responses inversely responding to CO(2) rises from 40 and 36% (for SD and SI, respectively) in experimental studies to 88 and 94% (for SD and SI, respectively) in fossil studies. The inconsistent experimental responses verify previous concerns involving this method, however the high percentage of fossil responses showing an inverse relationship clearly validates the method when applied over time scales of similar length. Furthermore, for all groups of observations, a positive relationship between CO(2) and SD/SI is found in only </=12% of cases. Thus, CO(2) appears to inversely affect stomatal initiation, although the mechanism may involve genetic adaptation and therefore is often not clearly expressed under short CO(2) exposure times.Experimental responses of SD and SI based on open-top chambers (OTCs) inversely relate to CO(2) less often than greenhouse-based responses (P<0.01 for both SD and SI), and should be avoided when experimental responses are required for CO(2) reconstructions. In the combined data set, hypostomatous species follow the inverse relationship more often than amphistomatous species (56 vs. 44% for SD; 69 vs. 32% for SI; P<0.03 for both comparisons). Both the SD and SI of fossil responses are equally likely to inversely relate to CO(2) when exposed to elevated versus subambient CO(2) concentrations (relative to today). This result casts doubt on previous claims that stomata cannot respond to CO(2) concentrations above present-day levels. Although the proportion of SD and SI responses inversely relating to CO(2) are similar, SD is more strongly affected by various environmental stresses, and thus SI-based CO(2) reconstructions are probably more accurate.

Journal Article↗
PubMed1983

Light and Stomatal Metabolism : II. Effects of Sulfite and Arsenite on Stomatal Opening and Light Modulation of Enzymes in Epidermis.

The effect of sulfite and arsenite on stomatal opening and light modulation of enzymes was examined in isolated epidermal strips of Pisum sativum L. var Little Marvel leaves. Sulfite or arsenite at 10 micromolar rapidly inhibited the stomatal opening process in light. Light activation of phosphoenolpyruvate carboxylase and NADP-malate dehydrogenase was completely diminished when the epidermal strips were incubated for 2 hours in light with either sulfite or arsenite at 10 micromolar. The data obtained suggest that the inhibition of stomatal opening by sulfite or arsenite in light might result from the inhibition of light modulation of key enzymes in guard cells.

Journal Article↗
PubMed1985

Effects of Nitrate Application on Amaranthus powellii Wats : II. Stomatal Response to Vapor Pressure Difference is Consistent with Optimization of Stomatal Conductance.

The hypothesis of optimal stomatal conductance predicts conductance should vary with changes of the vapor pressure difference between leaf and air (VPD) to keep the partial derivative of transpiration rate (E) with respect to assimilation rate (A) constant ( partial differentialE/ partial differentialA = lambda). Stomatal conductance of Amaranthus powellii Wats. decreased strongly with increasing VPD for leaves with high total leaf nitrogen concentrations; whereas, it decreased slightly with increasing VPD for leaves with low leaf nitrogen concentrations. The calculated value of partial differentialE/ partial differentialA was constant for leaves with high leaf nitrogen concentrations but was not constant for leaves with low leaf nitrogen concentrations. However, the predicted values of stomatal conductance, transpiration rate, and assimilation rate for a constant lambda = 220 moles H(2)O/mole CO(2) and the measured values fit the y = x line for all nitrate treatments. These data extend the experimental support for the optimal water use efficiency hypothesis for a C(4) plant grown under different nitrate concentrations.

Journal Article↗
PubMed1990

Rapid and Specific Modulation of Stomatal Conductance by Blue Light in Ivy (Hedera helix) : An Approach to Assess the Stomatal Limitation of Carbon Assimilation.

Low intensity (0.015 millimole per square meter per second) blue light applied to leaves of Hedera helix under a high intensity red light background (0.50 millimole per square meter per second red light) induced a specific stomatal opening response, with rapid kinetics comparable to those previously reported for stomata with ;grass type' morphology. The response of stomatal conductance to blue light showed a transient ;overshoot' behavior at high vapor pressure difference (2.25 +/- 0.15 kiloPascals), but not at low vapor pressure difference (VPD) (0.90 +/- 0.10 kilo-Pascal). The blue light-induced conductance increase was accompanied by an increase in net photosynthetic carbon assimilation, mediated by an increase in the intercellular concentration of carbon dioxide. Values of assimilation once the blue light-stimulated conductance increase reached steady state were less than those at the peak of the overshoot, but the ratios of assimilation to transpiration (A/E) and blue light-stimulated DeltaA/DeltaE were greater during the steady-state response than during the overshoot. These results indicate that significant stomatal limitation of assimilation can occur, but that this limitation may improve water use efficiency under high VPD conditions. Under high intensity red light, the decline in A/E associated with an increase in VPD was minimized when conductance was stimulated by additional low intensity blue light. This effect indicates that the blue light response of stomata may be important in H. helix for the optimization of water use efficiency under natural conditions of high irradiance and VPD.

Journal Article↗
PubMed1975

Histochemical studies in stomatal apparatus of Phaseolus mungo linn. IV. Mechanism of stomatal action.

The histochemical studies were carried out in the open and closed stomata of Phaseolus mungo leaves. Several enzymes like, Acid phospatase peroxidase, succinic dehydrogenase, phosphorylase, alkaline phosphatase, ATP-ase etc. were localized in the guard and subsidiary cells of epidermal peel. On the basis of cytochemical localization, enzyme activity was precisely interpreted. In the light of fluctuations in the localization, activities of different enzymes, an attempt is made to provide the functional interpretation of stomatal mechanism. We have attempted to correlat our observations in relation to diurnal metabolisms. Our studies suggest that starch-sugar inter-changes played a vital role in the stomatal regulation. We are also inclined to believe that besides guard cells, subsidiary cells also influenced the turgid conditions. A model based on available facts in collaboration with our own studies is presented which tends to explain the stomatal regulation.

Acid Phosphatase↗
PubMed2002

Comparison of the serum neutralization test and a competitive enzyme-linked immunosorbent assay for the detection of antibodies to vesicular stomatitis virus New Jersey and vesicular stomatitis virus Indiana.

A competitive enzyme-linked immunosorbent assay (C-ELISA) for the detection of antibodies against vesicular stomatitis virus New Jersey (VSV-NJ) and vesicular stomatitis virus Indiana (VSV-IN) was compared with the serum neutralization test (SNT) using 1,106 serum samples obtained from dairy cattle on sentinel study farms in the Poás region of Costa Rica. Kappa coefficients between the C-ELISA and the SNT were 0.8871 (95% confidence interval [CI]: 0.8587-0.9155) and 0.6912 (95% CI: 0.6246-0.7577) for the VSV-NJ and VSV-IN tests, respectively. These results indicate good to excellent agreement between the 2 tests under these conditions.

Animal Diseases↗
PubMed1995

[Veterinary dentistry (11). Feline gingivitis-stomatitis-pharyngitis complex. Chronic/recurrent stomatitis in cats].

This is the fourth article in a series on veterinary dentistry in cats. This article describes the clinical signs, possible investigations, and differential diagnosis of the gingivitis-stomatitis pharyngitis complex (GSP complex), a complex and frequently occurring disease. Strategies for the treatment of feline chronic stomatitis complex, which is frequently idiopathic, are presented.

Animals↗
PubMed1998

Assessing stomatitis: refinement of the Western Consortium for Cancer Nursing Research (WCCNR) stomatitis staging system.

The purpose of this study was to refine the Western Consortium for Cancer Nursing Research (WCCNR) stomatitis staging system. Fifty-six adult cancer patients were accrued. Using all eight descriptors, 96.4% of the participants were correctly staged. Using only lesions, colour and bleeding, however, 92.9% of the cases were correctly staged. Based on the findings of this study, the WCCNR stomatitis staging system has been shortened to include only lesions, colour and bleeding.

Adult↗
PubMed1980

Dark Opening of Stomates of Vicia faba in CO(2)-free Air: EFFECT OF TEMPERATURE ON STOMATAL APERTURE AND POTASSIUM ACCUMULATION.

Leaf epidermal strips of Vicia faba were floated on 10 millimolar KCl at temperatures of 10 to 45 C for several time periods in the dark. For the duration of the experiment, a jet of water-saturated, CO(2)-free air was passed over the strips. The width of the stomatal aperture was measured microscopically and the potassium content was estimated and expressed as the percentage of area of the guard cell pair stained with cobaltinitrite. Stomatal opening in the dark occurred at temperatures above 35 C. The high temperature aperture reduction previously observed at 45 C in the light was not observed in the dark. Potassium accumulation occurred at all temperatures except 10 C, with a maximum at 45 C, which appeared functionally different from that seen previously in the light.

Journal Article↗
PubMed1973

Primary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups.

The process of adsorption and RNA transcription by vesicular stomatitis virus (VSV) in cells has been followed by using highly labeled preparations of VSV virions. The initial transcription process (primary parental transcription) is rapid and takes about 4 min to develop complete transcripts of the input genome. The optimal cell temperature for in vivo transcription is between 36 and 39.5 C, although transcription can be detected at 18 C. No negative effect on primary parental transcription was obtained by the presence of actinomycin D, puromycin, or cycloheximide. Demonstrable primary transcription by selected temperature-sensitive mutants of all five complementation groups of VSV was obtained at either 31 and 34 C (permissive temperatures for virus production) or 39.5 C (nonpermissive temperature for virus development). VSV grown in hamster (BHK 21) or chicken embryo cells were more efficiently adsorbed and transcribed in BHK cells.

Adsorption↗
PubMed1974

Reconstitution of infectivity and transcriptase activity of homologous and heterologous viruses: vesicular stomatitis (Indiana serotype), Chandipura, vesicular stomatitis (New Jersey serotype), and Cocal viruses.

RNA TRANSCRIPTASE ACTIVITIES HAVE BEEN RECONSTITUTED FROM FRACTIONATED COMPONENTS OF FOUR RHABDOVIRUSES: vesicular stomatitis virus (VSV) (Indiana serotype), Cocal, VSV (New Jersey), and Chandipura viruses. Heterologous reconstitution of transcription has been observed between template and enzyme fractions of VSV Indiana and Cocal viruses but not to any significant extent between components of the other two viruses with VSV Indiana template or enzyme. Homologous reconstitution of infectivity has been obtained for each virus from their respective parts as well as heterologous reconstitution between Cocal and VSV Indiana components.

Arboviruses↗
PubMed1976

Characterization and translation of methylated and unmethylated vesicular stomatitis virus mRNA synthesized in vitro by ribonucleoprotein particles from vesicular stomatitis virus-infected L cells.

Ribonucleoprotein particles isolated from extracts of vesicular stomatitis virus (VSV) -infected L cells synthesized in vitro four classes of polyadenylated RNA sedimenting at 29S, 19S, 17S, and 13S. When synthesized in vitro in the presence of the methyl donor S-adenosyl methionine, these RNA species contained the following 5'-terminal structures: (i) m7G5ppp5'AmpAp(70%) ; (ii) m7G5'ppp5'AmpAmpNp (20%) and (iii) pppAp (10%). In the presence of the methylation inhibitor S-adenosylhomocysteine, however, the mRNA contained the 5'-terminal structures G5'ppp5'Ap (80%) and pppAp (20%). The mRNA's synthesized in vitro were translated in the homologous ascites and the heterologous wheat embryo cell-free systems. In both, the products were shown by sodium dodecyl sulfate gel electrophoresis and by immunoprecipitation to contain all five viral proteins, L, G, N, NS, and M. The presumed precursor to the G protein (G*) was also identified by fingerprint analysis. Methylated VSV mRNA was more active in protein synthesis than unmethylated mRNA in both the ascites system and the wheat embryo systems. Addition of S-adenosylmethionine stimulated translation of unmethylated mRNA in the wheat embryo but not in the ascites extract. S-adenosylhomocysteine, however, by preventing mRNA methylation inhibited the translation of unmethylated VSV mRNA in both systems. The mRNA methylating activity present in wheat embryo S-30 extracts was recovered in the ribosome-free supernatant fraction (S-150) and was insensitive to the protein synthesis inhibitor pactamycin.

Base Sequence↗
PubMed1977

RNA synthesis of vesicular stomatitis virus. VII. Complete separation of the mRNA's of vesicular stomatitis virus by duplex formation.

Full-length virion RNA and complementary mRNA's of vesicular stomatitis virus can be annealed to each other, digested with RNases, and then separated as five unique duplex RNA molecules on polyacrylamide slab gels. Similar RNA duplexes were detected whether mRNA or virion RNA was the radioactive component and whether the mRNA was synthesized in vitro or in vivo. The sharp banding pattern of these RNA molecules was dependent on treatment with RNase T2, suggesting that removal of poly(A) is necessary. Identification of the coding region contained in each RNA duplex was based on their previous identification as single-stranded mRNA on formamide-containing, polyacrylamide gels. Because the two smallest mRNA'S had not been previously separated, their identification was based on their in vitro transcriptional gene order. In the order of increasing mobilities on the slab gels, the RNA duplexes are identified as the hybrid of the region of the genome RNA hybridized to the complementary mRNA coding for the large protein, the glycoprotein, the nucleocapsid protein, the core-associated NS protein, and the matrix protein (L,G,N,NS, and M). Several lines of evidence support the presence of undegraded complete mRNA, excluding poly(A), in these RNA duplexes. Also, the two smallest mRNA's, separated by duplex formation, were denatured, and their individual oligonucleotide fingerprints were determined. From chemical length determinations, the molecular weights of the mRNA, minus poly(A), are 2.78 X 10(5) and 2.5 X 10(5), respectively, for the mRNA's of the NS and M proteins.

Cell Line↗
PubMed1977

Pseudotypes of vesicular stomatitis virus with the mixed coat of reticuloendotheliosis virus and vesicular stomatitis virus.

Vesicular stomatitis virus (VSV) forms pseudotypes with envelope components of reticuloendotheliosis virus (REV). The VSV pseudotype possesses the limited host range and antigenic properties of REV. Approximately 70% of the VSV, Indiana serotype, and 45% of VSV, New Jersey serotype, produced from the REV strain T-transformed chicken bone marrow cells contain mixed envelope components of both VSV and REV. VSV pseudotypes with mixed envelope antigens can be neutralized with excess amounts of either anti-VSV antiserum or anti-REV antiserum.

Antigens, Viral↗
PubMed1981

Vesicular stomatitis virus mRNA and inhibition of translation of cellular mRNA--is there a P function in vesicular stomatitis virus?

Infection of animal cells by vesicular stomatitis virus (VSV) results in inhibition of translation of cellular mRNA. We showed previously that, in BHK cells infected by the Glasgow isolate of VSV Indiana, this is due to competition during the initiation step of protein synthesis of viral and cellular mRNA for a constant, limiting number of ribosomes. We show here that infection of the same cells with the San Juan isolate of VSV resulted in a more rapid shutoff of host protein synthesis and that this was paralleled by a more rapid accumulation of viral mRNA. Extending our conclusion that shutoff is due to mRNA competition, we show further that the average size of polysomes translating viral and cellular mRNA was threefold smaller in cells infected by VSV San Juan than by VSV Glasgow, which, in turn, was about one-half that of uninfected cells. In all cases, cellular and viral mRNA's which encoded the same-sized polypeptides were found on the same-sized polysomes, a result indicating that the efficiency of translation of both types of mRNA's is about the same in the infected cell. Also, there was no preferential sequestration of viral or cellular mRNA's in ribonucleoprotein particles. Additional correlations between the levels of viral mRNA's and the inhibition of protein synthesis came from studies of three other wild-type VSV strains and also from studies with Vero and L cells. In particular, the rate of shutoff of L-cell protein synthesis after infection by any VSV isolate was slower than that in BHK cells, and this was correlated with a slower rate of accumulation of viral mRNA. VSV temperature-sensitive mutants which synthesized, at the nonper-missive temperature, no VSV mRNA failed to inhibit synthesis of cellular proteins. Stanners and co-workers (C. P. Stanners, A. M. Francoeur, and T. Lam, Cell 11:273-281, 1977) claimed that VSV mutant R1 inhibited synthesis of L cell protein synthesis less rapidly than did its parent wild-type strain HR. They concluded that this effect was due to a mutation in an unspecified VSV protein, "P." We found, in both L and BHK cells, that R1 infection resulted in a slightly slower inhibition of cellular mRNA translation than did HR infection and that this was correlated with a slightly reduced accumulation of VSV mRNA. The level of VSV mRNA, rather than any specific VSV protein, appeared to be the key factor in determining the rate of shutoff of host protein synthesis.

Animals↗
PubMed1971

Proteins of vesicular stomatitis virus and of phenotypically mixed vesicular stomatitis virus-simian virus 5 virions.

The identity of the glycoprotein of vesicular stomatitis virus (VSV) as the spike protein has been confirmed by the removal of the spikes with a protease from Streptomyces griseus, leaving bullet-shaped particles bounded by a smooth membrane. This treatment removes the glycoprotein but does not affect the other virion proteins, apparently because they are protected from the enzyme by the lipids in the viral membrane. The proteins of phenotypically mixed, bullet-shaped virions produced by cells mixedly infected with VSV and the parainfluenza virus simian virus 5 (SV5) have been analyzed by polyacrylamide gel electrophoresis. These virions contain all the VSV proteins plus the two SV5 spike proteins, both of which are glycoproteins. The finding of the SV5 spike glycoproteins on virions with the typical morphology of VSV indicates that there is not a stringent requirement that only the VSV glycoprotein can be used to form the bullet-shaped virion. On the other hand, the SV5 nucleocapsid protein and the major non-spike protein of the SV5 envelope were not detected in the phenotypically mixed virions, and this suggests that a specific interaction between the VSV nucleocapsid and regions of the cell membrane which contain the nonglycosylated VSV envelope protein is necessary for assembly of the bullet-shaped virion.

Amino Acids↗