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At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[Rhizopus microsporus strain UzLT-I--a thermotolerant producer of lipase].

The properties of the thermotolerant fungus Rhizopus microsporus strain UzLT-1--producer of lipolyptic enzymes are described. Optimal cultivation conditions--40 degrees, C, pH 4.5--Are determined. The lipolytic activity of the culture on the medium consisting of corn extract (2%), cotton-seed oil (1%) and water is 850 ml 0.1 n KOH per 100 ml culture liquid. The enzymic preparations of lipase have been precipitated by isopropanol and ammonium sulphate. The preparation precipitated by isopropanol shows its macimum activity at pH 4.2 and 7.8 and a temperature of 40--50 degrees C.

Culture Media↗
PubMedDate not supplied

[Biosynthesis of lipase by the mold fungus Rhizopus species, strain 3-3].

Cultivation of the microscopic fungus Rhizopus sp. str. 3-3--producer was investigated. Properties of lipase were studied. The technical preparation liparisopin G3x was obtained and examined. pH optimum of the preparation was within 6.0--7.0 and temperature optimum was 37 degrees C (olive oil used as substrate).

Hydrogen-Ion Concentration↗
PubMedDate not supplied

[Induced variability of the lipase producing fungus Rhizopus microsporus].

The most effective way for inducing mutants of Rhizopus microsporus with an elevated lipolytic activity is the combined action of nitrosomethyl urea and UV. Variants with the lipolytic activity of 3600--3700 units per 1 ml have been produced, thus being by 72--75% more effective than the parent culture.

Dose-Response Relationship, Drug↗
PubMedDate not supplied

[Effect of various procedures of storing the fungus Rhizopus microsporus UzLT-1, a lipase producer].

The effect of various storage procedures on the viability and synthesis of lipolytic enzymes by Rhizopus microsporus UzLT-1 was studied. The best procedures of producer storage proved to be regular passages and lyophilization. These procedures made it possible to maintain stable activity of lipolytic enzymes produced by the fungus for a long time. The fungal storage in vaseline oil or in a dried state was less effective due to significant losses of its lipolytic capacity.

Lipase↗
PubMedDate not supplied

[Effect of cultivation temperature on thermal stability of lipolytic enzymes of the fungus Rhizopus microsporus, UzLT-1].

The yield, activity and thermal stability of lipolytic enzymes obtained from the culture fluid filtrates of Rhizopus microsporus, UzLT-1, cultivated at 28, 38 and 48 degrees C (preparations I, II and III, respectively) were investigated. Maximal lipolytic activity was found in Prepartion II, and maximal yield and thermal stability in Preparation III. By disc electrophoresis and DEAE-cellulose chromatography, the presence of three lipolytically active enzymes in the preparations was demonstrated.

Kinetics↗
PubMedDate not supplied

[Effect of various nitrogen sources on lipase formation by Rhizopus microsporus].

The effect of organic and mineral nitrogen sources on the production of lipolytic enzymes and the accumulation of biomass was studied with Rhizopus microsporus UzLT-I. Addition of mineral nitrogen sources to the growth medium weakly stimulated synthesis of lipase by the fungus. The production of lipolytic enzymes was highest on media with organic nitrogen compounds, particularly yeast autolysate and fodder yeast cells (0.1: 0.25%). The lipolytic activity of the cultural broth on media with these nitrogen sources increased by 50% cf. the control.

Culture Media↗
PubMedDate not supplied

[Effect of metal ions on the lipolytic activity of Rhizopus microsporus].

Various metal ions have different effect on the lipolytic activity of Rhizopus microsporus in the course of cultivation on nutrient media having diverse composition. The fungus particulary requires metal ions for the production of lipase on a mineral medium. Additional introduction of microelements into a medium containing maize extract has no significant effect on the lipolytic activity. Active biosynthesis of lipase by the culture requires zinc.

Enzyme Induction↗
PubMed1979

Cutaneous phycomycosis. Report of three cases with identification of Rhizopus.

Three cases of pustular and ulcerative cutaneous phycomycosis developed postoperatively in orthopedic patients following exposure to a contaiminated surgical adhesive (Elastoplast). A review of the pertinent literature revealed no other similar series of reported cases. The epidemiologic investigation of these cases is discussed. This experience demonstrates that with appropriate exposure, normal saprophytes can invade the skin in immunocompetent patients, producing active and invasive lesions.

Adolescent↗
PubMed1977

Homology among acid proteases: comparison of crystal structures at 3A resolution of acid proteases from Rhizopus chinensis and Endothia parasitica.

The molecular structures of two fungal acid proteases at 3 A resolution have been compared, and found to have similar secondary and tertiary folding. These enzymes are bilobal and have a pronounced cleft between the two lobes. This cleft has been identified as the active site region from inhibitor binding studies. The results of the comparison are discussed in terms of homology among the acid proteases in general.

Ascomycota↗