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Results for “Receptors, Colony-Stimulating Factor”

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PubMed1978

Effect of neutral proteases from human granulocytes on colony forming cells in vitro.

Marked inhibition of colony formation is observed after incubation of mouse and human bone marrow cells with the human granulocytic neutral proteases elastase and chymotrypsin as well as with pancreatic chymotrypsin. The corresponding enzymes inactivated with diisopropylfluorophosphate were almost inactive. Incubation of different colony inducing agents either resulted in no change or in an increase of their colony stimulating activity. The data suggest a direct proteolytic action of the proteases on colony forming cells which may alter receptor sites for colony stimulating activities.

Animals↗
PubMed1977

The regulation system of granulopoiesis.

Extracts containing the granulocytic chalone (GCh) have been prepared from bone marrow conditioned medium by ultrafiltration and column chromatography. This preparation selectively inhibits myeloid cells and does not affect other, e.g., lymphoid cells. The action on myelopoiesis seems to include all mitotically competent immature granulocytes as indicated also by an inhibitory effect on bone marrow colony formation in agar. Results obtained by this technique and by applying mild trypsinization of bone marrow cells prior to testing chalone responsiveness indicate that GCh acts via membrane receptors. Finally the apparent antagonistic role of colony stimulating factor and GCh as regulators of granulopoiesis is discussed and a model of the regulation system is described.

Bone Marrow↗
PubMed1978

Hematopoiesis on cellulose ester membranes (CEM). I. Functional characteristics of cells comprising the hematopoietic microenvironment.

Cellulose ester membranes (CEM) implanted into the peritoneal cavity of mice rapidly became coated with cells of peritoneal origin. Up to 56% of the cells, at a peak point 3-5 days after implantation, showed cell membrane receptors for complement and cytophilic immunoglobulin. A similar proportion of cells from CEM phagocytized yeast particles in vitro. When studied in situ, rosettes with C3b and IgG coated erythrocytes were formed by 23% of the cells coating CEM. A decreasing percentage of cells with monocyte-macrophage characteristics were detected between 5 and 17 days. CEM removed at 2 or 6 weeks after peritoneal implantation enriched tissue cultured media with colony stimulating factor which supported the growth of granulopoietic colonies in softagar culture. Mice given 59iron and 99technetium sulfur colloid i.v. showed substantial uptake of both isotopes by the CEM but the 59iron uptake could not be suppressed by hypertransfusion. Surface hematopoietic colony formation on CEM was studied 1-14 days after implantation. A peak colony number occurred at 5 days and the fell off slightly by 2 weeks. These studies indicate that the hematopoietic microenvironment of peritoneally implanted CEM contains a major sub-population of cells with monocytemacrophage features. The hematopoietic microenvironment was well-maintained even though the percentage of monocyte-macrophage marked cells decreased indicating that the microenvironment is not solely dependent upon monocyte-macrophages.

Animals↗