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PubMed1975

[Qualitative and quantitative studies on the corpus geniculatum laterale (Cgl) of the laboratory rat. IV. Qualitative structure analysis of the pars dorsalis an on India-ink-injected Nissl preparation].

1. The histological organization of the rat dorsal lateral geniculate nucleus (Cgld) has been analysed in Nissl-preparations following vessel injection with india ink. 2. The findings have been collected by means of a non-automatically visual scanning method. Every point is defined by 3 coordinates. 3. Different parameters have been collected in every point: the number of the 5 structural elements of the nervous tissue (neurons, fragments of capillaries, nuclei of astrocytes, oligodendrocytes and microglia) and the hit points per structural element. 4. In addition the differentiation of about 15500 neurons in relay neurons and interneurons has been carried out. The ratio of the 2 types of neurons is about 14:1. 5. The collected values have been statistically analysed by correlation, regression and discriminatory analyses. 6. The results relate to the correlation between the structural elements and the rostrocaudal, lateromedial and ventrodorsal directions as well as the structural elements with another. 7. At 5 levels the Cgld has been mapped on the basis of neuron size, neuron density, capillary density, density of the 3 types of glial nuclei and the relative number of hit points per structural element. 8. In the Cgld we distinguish 2 portions regarding the neuron size. The mediocellular portion occupies the lateral, ventral and caudal parts and encloses a magnocellular portion like a coat. 9. The distribution pattern of the interneurons does not correlate with the size of neurons. 10. The capillaries tend to have a greater density in the lateral area of the Cgld. 11. The correlation and regression coefficientes and the distribution pattern of the astrocytes and oligodendrocytes suggest that both glial types show a reciproke ratio in the Cgld. 12. The distribution pattern of the microglia is relatively uniform. 13. Mapping the Cgld we have got a model conception of its structural condition corresponding with the common mammalian scheme. 14. By the discriminatory analysis zones of input and fields of projection have been discriminated. It was possible to discriminate the ipsi- and contralateral input zones, but not the binocular and monocular projection fields. 15. The discriminatory analysis allows to show the collective biological effect of all structural elements in a uniform mathematical value.

Animals↗
PubMed2002

Understanding and evaluating qualitative research.

Qualitative research aims to address questions concerned with developing an understanding of the meaning and experience dimensions of humans' lives and social worlds. Central to good qualitative research is whether the research participants' subjective meanings, actions and social contexts, as understood by them, are illuminated. This paper aims to provide beginning researchers, and those unfamiliar with qualitative research, with an orientation to the principles that inform the evaluation of the design, conduct, findings and interpretation of qualitative research. It orients the reader to two philosophical perspectives, the interpretive and critical research paradigms, which underpin both the qualitative research methodologies most often used in mental health research, and how qualitative research is evaluated. Criteria for evaluating quality are interconnected with standards for ethics in qualitative research. They include principles for good practice in the conduct of qualitative research, and for trustworthiness in the interpretation of qualitative data. The paper reviews these criteria, and discusses how they may be used to evaluate qualitative research presented in research reports. These principles also offer some guidance about the conduct of sound qualitative research for the beginner qualitative researcher.

Comprehension↗
PubMed2000

Study design in qualitative research--2: Sampling and data collection strategies.

In two prior papers in our series on qualitative research [Frankel & Devers (2000a, 2000b) Qualitative research: a consumer's guide, Education for Health, 13, 113-123; Frankel & Devers (2000) Study design in qualitative research-1: developing research questions and assessing research needs, Education for Health, 13, 251-261], we examine two critical issues in qualitative research design: sampling, including identifying and negotiating access to research sites and subjects, and data collection and management. We describe these two key steps in the qualitative research design process, discuss challenges that often emerge when pursuing these steps, and provide guidelines for addressing them. Qualitative research most often uses "purposive," rather than random, sampling strategies. A good understanding of these sampling strategies and why they are used is central to designing a credible qualitative study. In addition, given the real-world context in which most qualitative research is carried out, identifying and negotiating access to research sites and subjects are critical parts of the process. We also provide suggestions for developing and maintaining productive and mutually satisfying research relationships with sites and subjects. Finally, data collection and management are often neglected subjects in qualitative research. We offer practical advice on how to collect and manage qualitative data, including factors to consider when deciding how structured the data collection process should be, the pros and cons of audio- and/or videotaping compared with note-taking, and tips for writing up field notes and document management. A forthcoming, final paper in the series will focus on qualitative data analysis and the publication of qualitative research results.

Journal Article↗