Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Periplaneta”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 37 records · Page 2Linked to original sources
PubMed1998

Molecular cloning of cDNA for p10, a novel protein that increases in the regenerating legs of Periplaneta americana (American cockroach).

Previously, we purified a protein with a molecular mass of 10 kDa (p10) that increases transiently during the regeneration of legs in the nymphal American cockroach Periplaneta americana, and showed that it is localized exclusively in the cytosol and on the external side of the newly formed epidermis of the regenerating legs [Nomura, A. et al. (1992) Int. J. Dev. Biol. 36, 391-389]. We isolated p10 cDNA and analyzed the expression of the p10 gene. The results indicated that p10 is synthesized as a precursor protein with a putative prosegment including a signal sequence at its N-terminal. The deduced amino acid sequence of p10 showed 53% and 47% identities with those of A10 (a Drosophila antennal protein) and CLP-1 (a moth, Cactoblastis cactorum labial palp protein), respectively. Expression of the p10 gene was shown to be significantly enhanced in regenerating Periplaneta legs. Immunoblotting analysis revealed that p10 was expressed not only in the regenerating legs, but also in the antennae and heads of nymphal and adult cockroaches.

Amino Acid Sequence↗
PubMed1998

Cloning of cDNA for regenectin, a humoral C-type lectin of Periplaneta americana, and expression of the regenectin gene during leg regeneration.

We isolated cDNA for regenectin, a C-type lectin of the American cockroach (Periplaneta americana), and analysed expression of the regenectin gene in the regenerating legs. Regenectin was found to be a member of the Periplaneta lectin-related protein family. We found that the regenectin gene was expressed specifically in the epidermal cells of the newly formed regenerating legs. Together with our previous results, these results suggest that regenectin is synthesized by epidermal cells, secreted into the regenerating leg saccule, and assembles around myoblasts to form leg muscle fibers in situ.

Amino Acid Sequence↗
PubMed2003

The large conductance Ca2+-activated potassium channel (pSlo) of the cockroach Periplaneta americana: structure, localization in neurons and electrophysiology.

Voltage-activated, Ca2+-sensitive K+ channels (BK or maxi K,Ca channels) play a major role in the control of neuronal excitability. We have cloned pSlo, the BK channel alpha subunit of the cockroach Periplaneta americana. The amino acid sequence of pSlo shows 88% identity to dSlo from Drosophila. There are five alternatively spliced positions in pSlo showing differential expression in various tissues. A pSlo-specific antibody prominently stained the octopaminergic dorsal unpaired median (DUM) neurons and peptidergic midline neurons in Periplaneta abdominal ganglia. HEK293 cells expressing pSlo exhibit K+ channels of 170 pS conductance. They have a tendency for brief closures, exhibit subconductance states and show slight inward rectification. Activation kinetics and voltage dependence are controlled by cytoplasmic [Ca2+]. In contrast to dSlo, pSlo channels are sensitive to charybdotoxin and iberiotoxin. Mutagenesis at two positions (E254 and Q285) changed blocking efficacy of charybdotoxin. In contrast to pSlo expressed in HEK293 cells, native IbTx-sensitive K,Ca currents in DUM and in peptidergic neurons, exhibited rapid, partial inactivation. The fast component of the K,Ca current partly accounts for the repolarization and the early after-hyperpolarization of the action potential. By means of Ca2+-induced repolarization, BK channels may reduce the risk of Ca2+ overload in cockroach neurons. Interestingly, the neurons expressing pSlo were also found to express taurine, a messenger that is likely to limit overexcitation by an autocrine mechanism in mammalian central neurons.

Alternative Splicing↗
PubMed2004

Allergenic characterization of tropomyosin from the dusky brown cockroach, Periplaneta fuliginosa.

Household arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach, Periplaneta fuliginosa. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot analyses for the dusky brown cockroach were performed with Blattella germanica and Dermatophagoides farinae allergic sera. cDNA encoding tropomyosin, which is a well known cross-reactive pan-allergen, was cloned by reverse transcriptase PCR, and recombinant protein was produced by using a pET-28b expression system. Native tropomyosin was purified by ammonium sulfate fractionation and electroelution. The immunoglobulin E (IgE) reactivities of native and recombinant tropomyosins were compared by an ELISA inhibition study. All 30 sera tested showed P. fuliginosa-specific IgE, and the IgE-binding reactivity of the P. fuliginosa extract was inhibited as much as 79.4% by a B. germanica extract and as much as 63.3% by a D. farinae extract. The deduced amino acid sequence of cloned cDNA was identical with that of Periplaneta americana tropomyosin (98.5% nucleotide sequence identity). Seven of 26 (26.9%) allergic sera had IgE specific for recombinant protein, and the maximum inhibition of P. fuliginosa-specific IgE achieved with recombinant tropomyosin was 37.7% at an inhibitor concentration of 10 microg/ml. Native tropomyosin inhibited the binding of IgE to the P. fuliginosa, B. germanica, and D. farinae extracts by 65.0, 51.8, and 39% at an inhibitor concentration of 1 microg/ml. P. fuliginosa appears to possess allergens that are highly cross-reactive with allergens of B. germanica and D. farinae. Tropomyosin was found to be a major allergenic component accounting for the cross-reactivity between cockroaches and dust mites.

Adolescent↗
PubMed1995

Water and carbon dioxide loss from the cockroach Periplaneta americana (L.) measured using radioactive isotopes.

Tritiated water (THO) loss from Periplaneta americana is used to measure cuticular permeability (Pd). In dry air, following various periods of predesiccation, the values of Pd lie around 0.5 x 10(-9) ms-1, with the lowest values below 0.3 x 10(-9) ms-1, close to the lowest permeabilities previously reported. There is no obvious relationship between permeability and initial mass of the insect. A significant lowering of Pd was found after 72 h of predesiccation. A marked sudden decrease in permeability occurred when the airstream flowing over an insect was changed from humid air to dry air. Labelling the bicarbonate pool using 14CO3(2-) enabled the output of CO2 to be estimated. An average output of about 390 microliters g-1 h-1 was found. Simultaneous measurement of THO and 14CO2 loss showed that tracheal water loss is only a small component of total water loss, with an average value of 3.8%. The total water loss can therefore be used to make close estimates of cuticular permeability in Periplaneta americana. 2min sampling periods clearly show the pulsatile nature of 14CO2 release but, even when the effect is most discernible, the periodic release of CO2 has only a small effect on water loss.

Animals↗
PubMed1997

Pharmacology of skeletal muscle GABA-gated chloride channels in the cockroach Periplaneta americana.

The pharmacology of -aminobutyric acid (GABA)-gated chloride channels of the coxal levator (182c,d) muscle of the cockroach Periplaneta americana has been investigated and the data compared with similar findings for the cell body of the cockroach fast coxal depressor motor neurone (Df). Muscle GABA receptors resembled those of the motor neurone cell body in their sensitivity to picrotoxinin and insensitivity to bicuculline. However, muscle GABA receptors were insensitive to the neuronal GABA receptor agonists isoguvacine (10(-4) mol l-1) and 3-aminopropane sulphonic acid (10(-3 )mol l-1). The benzodiazepine flunitrazepam, which at 10(-6 )mol l-1 greatly enhances the amplitude of the motor neurone GABA-induced responses, failed to affect muscle responses to GABA when tested at the same and at a higher (10(-4 )mol l-1) concentration. The convulsant t-butylbicyclophosphorothionate was a weak antagonist of cockroach muscle GABA receptors, whereas several cyclodienes were much more effective antagonists. Thus, studies using a benzodiazepine and several convulsant antagonists reveal differences in the pharmacology of muscle and neuronal GABA receptors of the cockroach Periplaneta americana.

Animals↗
PubMedDate not supplied

[Correlation between Blatella germanica and Periplaneta americana in allergic patients].

BACKGROUND: It has been identified shared allergens from whole body between Blatella germanica and Periplaneta americana. It has been reported high correlation between both cockroaches. OBJECTIVE: To estimate correlation between skin test for two major species of intradomiciliary cockroaches. METHODS: It was included patients with clinical diagnostic of allergy pathology whom attended to external consult of Allergy and Clinical Immunology Service of the Hospital Universitario de Puebla in a period of six months, prick skin tests with standardized antigens of Blatella germanica and Periplaneta americana were made. RESULTS: One hundred twenty patients were enrolled in the study, 54.2% were women and 45.8% were men, with a mean age of 15.7 years old. The correlation coefficient between Germany cockroach and American cockroach to erythema was 0.49 and to papule was 0.40. CONCLUSION: The outcomes showed a moderate correlation or a considerable relation between both cockroaches whether erythema or papule. Based on the results presented, we suggest to evaluate both antigens in order to improve diagnostic accuracy.

Adolescent↗
PubMed2002

Temperature acclimation in overwintering nymphs of a cockroach, Periplaneta japonica: walking on ice.

Nymphs of the Yamato cockroach, Periplaneta japonica, showed a seasonal change in the ability to move at low temperature. Laboratory-reared nymphs buried in ice for 15 min-3 h took about 600 s to get up at 25 degrees C after being placed with their dorsal surface on the floor of a Petri dish. The mean time to get up at 25 degrees C (recovering time) after 15 or 60-min burial in ice was relatively long in early autumn, decreased rapidly in autumn, reached a minimal level in winter (<100 s) and increased in spring. Temperature was responsible for this seasonal change in physiology, and neither photoperiod nor diapause status was important. Recovering time depended on the acclimation temperature and its duration. For a 10-day exposure in a range of 0-25 degrees C, the lower the temperature the shorter the recovering. Longer periods of acclimation at these temperatures tended to shorten recovering time. The acquisition of the ability to move on ice is a seasonal phenomenon unique to P. japonica. No comparable response was found in three other cockroaches including two sub-tropical species (Periplaneta americana and Opisthoplatia orientalis) and a temperate species (Blattela nipponica).

Journal Article↗
PubMed1995

Octopaminergic dorsal unpaired median (DUM) neurones innervating the colleterial glands of the female cockroach Periplaneta americana

The musculature of the colleterial glands receives innervation from branch 4B4a of the nerves designated 4B, which arise from the posterior part of the terminal abdominal ganglion in the female cockroach Periplaneta americana (L). Using Methylene Blue staining, the gross anatomy of the colleterial gland innervation has been described. Cobalt backfilling via branch 4B4 of nerve 4B revealed about 21 dorsal unpaired median (DUM) neurones located on both median and posterior parts of the terminal abdominal ganglion. Octopamine immunohistochemistry has shown that at least 15 octopamine-immunoreactive DUM neurones from median and posterior groups projected via branch 4B4a to the left and right colleterial glands. These data, together with results reporting the presence of octopamine-immunoreactive branches supplying these colleterial glands, make octopaminergic DUM neurones suitable candidates to modulate the muscle activity of the colleterial glands in female Periplaneta americana.

Journal Article↗
PubMedDate not supplied

Metabolic transformation of the brassinosteroid 24-epi-castasterone by the cockroach Periplaneta americana.

After feeding of 24-epi-castasterone to the cockroach Periplaneta americana an organ-specific epimerization of the brassinosteroid to 2,24-diepi-castasterone could be detected in female insects. The metabolite being observed only in the ovaries and not in the testes of the insect was identified by GC/MS in comparison with a synthesized authentic sample. Contrary, 24-epi-brassinolide is not metabolized in the sexual organs of Periplaneta americana. This is the first evidence of a metabolic transformation of a brassinosteroid in insects.

Animals↗
PubMed1991

Molecular cloning of cDNA for lipopolysaccharide-binding protein from the hemolymph of the American cockroach, Periplaneta americana. Similarity of the protein with animal lectins and its acute phase expression.

A previous paper described the purification of a calcium-dependent lipopolysaccharide-binding protein from the hemolymph of Periplaneta americana (Jomori, T., Kubo, T., and Natori, S. (1990) Eur. J. Biochem. 190, 201-206). This paper describes the molecular cloning and characterization of cDNA for the LPS-binding protein. This protein was found to have a carbohydrate-recognition domain at its carboxyl terminus containing amino acid sequences that are conserved in various mammalian C-type lectins. It was also shown to contain an N-linked carbohydrate chain, and the amino acid residue carrying this chain was assigned as Asn at position 56 (23rd amino acid residue from the amino terminus). Northern blot analysis revealed the presence of multiple mRNAs that hybridized with this cDNA and transient increases in their content after injection of Escherichia coli into adult Periplaneta, suggesting that the LPS-binding protein plays a role in the acute phase response of this insect.

Acute-Phase Proteins↗
PubMed1981

Optic lobe neurosecretory cells in Poekilocerus pictus (Orthoptera), Periplaneta americana (Dictyoptera), Belostoma indicum (Hemiptera), Polistes hebraeus (Hymenoptera) and Sandrocottus de jeani (Coleoptera).

Neurosecretory cells in the optic lobes of 5 insects viz. Poekilocerus pictus (Orthoptera), Periplaneta americana (Dictyoptera) Belostoma indicum (Hemiptera), Polistes hebraeus (Hymenoptera) and Sandrocottus dejeani(Coleoptera) have been described. They occur in the distal optic chiasma of the optic lobes in the form of a cone-shaped concentration with two arms, a dorsal and a ventral. The number of neurosecretory cells in the optic lobes varies from species to species viz., about 150 in Poekilocerus, 115 in Periplaneta, 45 in Belostoma, 90 in Polistes and 70 in Sandrocottus. They are mostly monopolar but occasionally bipolar and stain green with PAF and red or sometimes deep purple with Heidenhain's Azan technique. The axons of the monopolar cells run into the optic lobe or the brain, while those of the bipolar cells form a sort of two way connection one end going to the eye and the other to the brain. Neurosecretory cells in all the insects described above have synchronous secretory cycles.

Animals↗
PubMed1994

Comparative susceptibility of Periplaneta americana (L) to five pyrethroid insecticides.

Five pyrethroids namely, lambda-cyhalothrin, permethrin, deltamethrin, cyfluthrin and alpha-cypermethrin were evaluated using adult male and female cockroaches, Periplaneta americana (L). The American cockroaches were exposed for 10 minutes to glass jars treated with different concentrations of the five pyrethroids. The cockroaches were susceptible to all five pyrethroids and the susceptibility based on LC50 and LC50 were observed to be in the following order: lambda-cyhalothrin > permethrin > deltamethrin > cyfluthrin > alpha-cypermethrin The results showed that lambda-cyhalothrin was the most effective and alpha-cypermethrin was the least effective against Periplaneta americana (L).

Animals↗
PubMed1996

Anatomy and targets of Dorsal Unpaired Median neurones in the Terminal Abdominal Ganglion of the male cockroach Periplaneta americana L.

The morphology of the Dorsal Unpaired Median (DUM) neurones in the Terminal Abdominal Ganglion (TAG) of the adult male cockroach Periplaneta americana were described based on wholemount preparations and paraffin sections and by using anterograde and retrograde cobalt mapping, octopamine-like immunohistochemistry, and double immunofluorescence technique with both conjugated gamma-aminobutyric acid (GABA) and octopamine antisera. Among 60 +/- 6 neurones with large somata (diameter 40 to 60 microns) on the dorsal midline surface of the TAG that were stained with toluidine blue, about 36 efferent DUM neurones exhibited octopamine-like immunoreactivity. The DUM neurones were arranged in three clusters (anterior, median and posterior) corresponding to the 7th-11th abdominal ganglia of the fused TAG. Anterior efferent DUM neurones with one, two, and four pairs of lateral neurites entered segmental nerves VIIB; VIIB and phallic nerves; IXB and phallic nerves; VIIIA, IXA, X, and IX, respectively. Three octopamine-like immunoreactive DUM neurones innervating heart chambers via segmental nerves (VIIA, VIIIA, and IXA) in the last abdominal segments occurred within abdominal ganglia 7, 8, and 9. Together with octopamine-like immunoreactive efferent DUM neurones, GABA-like immunoreactive dorsal midline neurones with small somata (10 to 20 microns) also occurred within the median group. The spatial distribution of DUM neurones in the TAG suggested that they had their origins in the median neuroblast, as for DUM neurones in the grasshopper.

Abdomen↗
PubMed1996

Influence of receptor axons on the formation of olfactory glomeruli in a hemimetabolous insect, the cockroach Periplaneta americana.

The embryonic development of the hemimetabolous insect Periplaneta americana requires approximately 31 days. Deafferentation experiments were used to investigate the role of ingrowing receptor axons during embryogenesis, specifically their influence 1) on the subdivision of the antennal lobe neuropil into glomeruli, 2) on the morphology and number of glial cells, and 3) on the arborization pattern of central neurons. The flagellum of one antenna was removed from embryos at different developmental stages starting with day 10. Subsequently, they were raised in culture until a total age of 26 days. At day 10, the deutocerebrum has received only a very small number (ca. 0.4%) of antennal receptor axons; deafferentation at this stage allowed us to deprive the deutocerebrum of approximately 99% of its normal antennal input. Deafferentation has marked effects on the organization of the antennal lobe neuropil. The deafferented lobe is reduced in volume compared to the control side; the characteristic glomeruli are missing. During normal development glomeruli are formed between day 19 and 22, first in dorsal and then in ventral antennal lobe regions. By removing the antenna before day 20, their formation is disturbed in all parts of the antennal lobe. If deafferentation is performed after stage 20, glomeruli persist in dorsal regions, but are missing in ventral regions. On day 24 or later, glomeruli in both dorsal and ventral regions are unaffected by deafferentation. Glial cells continue to extend fine processes into the neuropil in the absence of ingrowing receptor axons. The number of glial cells is reduced compared to control lobes. Multiglomerular local interneurons and other gamma-amino butyric acid-immunoreactive neurons, as well as projection neurons, fail to develop glomerular arborization patterns in antennal lobes deprived of sensory axons.

Afferent Pathways↗
PubMed1999

Quantification of periviscerokinin-1 in the nervous system of the American cockroach, Periplaneta americana. An insect neuropeptide with unusual distribution.

This study was undertaken to reveal the quantitative distribution of the insect neuropeptide periviscerokinin-1 (Pea-PVK-1) in the central nervous system of Periplaneta americana and to demonstrate that neurons stained in a previous immunohistochemical study contain authentic Pea-PVK-1. For this, we combined ELISA, HPLC, and MALDI-TOF mass spectrometry. The high specificity of the used antiserum enabled the quantification of Pea-PVK-1 in unseparated tissue extracts. No cross-reactivities with other insect neuropeptides were detected in ELISA. Only two immunoreactive fractions, coeluting with synthetic Pea-PVK-1 in its oxidized and nonoxidized form, were found in HPLC-separated extracts of the brain, suboesophageal ganglion, metathoracic ganglion, second abdominal ganglion with or without perisympathetic organ, and terminal ganglion. By using MALDI-TOF mass spectrometry, we were able to confirm the existence of authentic Pea-PVK-1 in these fractions. The abdominal perisympathetic organs contained 6.3 pmol Pea-PVK-1 per animal; another 1.3 pmol were found in the abdominal ganglia. More than 90% of the total 8.2 pmol in the central nervous system was found in the abdominal ganglia and their perisympathetic organs. The corpora cardiaca and corpora allata did not contain immunoreactive material, suggesting that Pea-PVK-1 is not released by the cephalic neurohaemal system. The quantitative distribution of Pea-PVK-1 differs considerably from that of other known insect neuropeptides.

Animals↗
PubMed2000

Cloning of vitellogenin cDNA of the American cockroach, Periplaneta americana (Dictyoptera), and its structural and expression analyses.

A cDNA expression library constructed from poly (A)(+) RNA prepared from vitellogenic female fat body cells of the American cockroach, Periplaneta americana (Dictyoptera) was screened using a polyclonal antiserum against the 100-kD polypeptide(s) from the egg extract. A partial Vg cDNA clone was obtained and sequenced. The 5' end portion of the cDNA was then obtained by the RACE method, cloned, and sequenced. The combined complete Vg cDNA was 5,854 bp long and contained a single ORF encoding 1,896 amino acids. The entire deduced amino acid sequence was aligned confidently with those of the known insect Vgs. A GL/ICG motif, a number of cysteines at conserved locations following this motif, and a DGXR motif upstream of the GL/ICG motif were present near the C-terminal. The chemically determined N-terminal amino acid sequence of the 170-kD polypeptide from the egg extract completely matched the deduced sequence starting from just after one of the consensus (RXXR) cleavage sites, indicating the occurrence of post-translational cleavage in the fat body cells. The Vg gene begins to be expressed in the 2-day-old adult female fat body cells but is never expressed in ovaries or in male fat body cells. Hemolymph Vg was first detected by immunoblotting in 4-day-old adult females, 2 days after the beginning of gene expression. Western blot analysis of major yolk polypeptides in nine cockroach species belonging to the two superfamilies, Blattoidea and Blaberoidea, using the antisera against P. americana major yolk polypeptides showed that the similarities in Vn antigenicity are basically limited to within a superfamily.

Amino Acid Sequence↗
PubMed2002

Characterization of cockroach (Periplaneta americana) fat body phospholipase A(2) activity.

A phospholipase has been identified in the fat body of the American cockroach, Periplaneta americana, which removes fatty acid from the sn-2 acyl position of an artificial substrate. The enzyme has been characterized using a crude preparation obtained by low-speed centrifugation of the homogenized tissue. With 1-hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphocholine as the substrate, the K(m) has been estimated to be 1.17 microM and the v(max) 113.5 pmol/min/mg protein. The phospholipase has a pH optimum close to 7 and shows maximal activity at 50 degrees C. Activity of the phospholipase has been determined in cytosolic and plasma membrane fractions. The specific activity of the latter fraction is approximately twice that of the cytosol. The enzyme in both fractions is Ca(2+)-independent. Arch.

Animals↗