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Results for “Pasteurella multocida”

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PubMed1990

Reduced microbicidal activity of peripheral mononuclear phagocytic cells infected with Pasteurella multocida.

Pasteurella multocida inhibits the uptake and killing of Candida albicans and P. multocida by avian mononuclear phagocytic cells. The toxic outer membrane protein of P. multocida, which has been previously described, also inhibited the uptake and killing of C. albicans. Antibody specific for the toxic outer membrane protein reversed this effect resulting not only in an increase in uptake of C. albicans and P. multocida, but also in intracellular killing of P. multocida. This antibody, however, only partially restored killing of C. albicans. These data support the hypothesis that P. multocida is capable of intracellular survival in avian mononuclear phagocytic cells and that the toxic outer membrane protein is totally or partly responsible for this occurrence.

Animals↗
PubMed1980

Articular and skeletal infections caused by Pasteurella multocida.

Pasteurella multocida infections of joints and bones generally occur in individuals who have contact with cats or dogs. Osteomyelitis usually follows penetrating trauma such as an animal bite. Septic arthritis tends to occur in patients who have preexisting inflammatory joint disease, especially if a systemic condition which is known to predispose to infection is present. The principles of therapy for septic arthritis or osteomyelitis are no different from those which have been established for other infecting organisms. Although P multocida is susceptible in vitro to penicillin, treatment of septic arthritis with this drug is still associated with a slow therapeutic response.

Anti-Bacterial Agents↗
PubMed1990

Sequence analysis of the potent mitogenic toxin of Pasteurella multocida.

Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no evidence for a signal sequence. The gene had a low G + C base ratio which differs from the rest of the Pasteurella genome. There was no significant homology with other known proteins, although a motif found in certain bacterial toxins which are ADP-ribosyl transferases is present. A recombinant expressing only part of the PMT gene was not mitogenic.

Amino Acid Sequence↗
PubMed1980

Late postoperative infection caused by Pasteurella multocida.

Pasteurella multocida is often found in local infections after animal bites. Septic conditions however are rare. A case of late infection by P. multocida at the site of the osteosynthesis 1 year after a primarily healed pertrochanteric fracture is described. The patient had a cat. After extraction of the plate and screws and treatment with penicillin the infection healed uneventfully.

Animals↗
PubMed1985

Acute meningoencephalomyelitis in a rabbit infected with Pasteurella multocida.

Pasteurella multocida was isolated in pure culture from the optic chiasm of a rabbit that was euthanatized subsequent to acute development of neurological signs. Histopathologically, there was meningoencephalomyelitis, bilateral otitis interna, retrobulbar cellulitis, optic neuritis and iritis. The ocular involvement, severity of the spinal lesions and the lack of otitis media was unusual.

Acute Disease↗
PubMedDate not supplied

Solubilization of membrane-associated cross-protection factor(s) of Pasteurella multocida.

Pasteurella multocida harvested from the blood of turkeys dying of experimental fowl cholera were purified by centrifugation and lysed. The soluble and membrane-associated components of the bacteria were separated by centrifugation. Nonionic (octylglucoside) and zwitterionic (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate; CHAPS) detergents were tested for their abilities to solubilize the cross-protection factor(s) (CPF) from the membrane-associated component. Protection studies in turkeys showed that optimum solubilization was by 1.0% octylglucoside and 0.5% CHAPS. Antibodies from turkeys made against solubilized membrane-associated CPF passively cross-protected poults against challenge. Ion exchange chromatography of detergent-solubilized CPF resulted in elution of two protein-containing peaks, each of which conferred active immune protection.

Animals↗
PubMed1999

Reduced pH causes structural changes in the potent mitogenic toxin of Pasteurella multocida.

Pasteurella multocida toxin is a potent mitogen that is believed to act intracellularly. On transverse urea gradient gels at pH 8.0 the toxin displayed one major unfolding transition at 4 M urea. However, at pH 6.1 the unfolding transition took place at 3.5 M urea. Circular dichroism spectra also indicated that a structural change took place at acidic pH. In addition it was found that the toxin that had been denatured in 8 M urea refolded in solution with a high recovery of biological activity. These findings are discussed in terms of the likely domain structure of the P. multocida toxin.

3T3 Cells↗
PubMed1994

Iron supply of Pasteurella multocida and Pasteurella haemolytica.

Pasteurella multocida and Pasteurella haemolytica do not produce hydroxamate- or phenolate type siderophores. However, transport- and utilization systems could be detected for the well known siderophores ferrioxamine B, E, G, rhizoferrin and the intermediate 2,3-dihydroxybenzoic acid by means of cross-feeding tests in both Pasteurella species. Enterobactin and ferrichrome did not feed any of the Pasteurella strains tested. Additionally, alpha-ketoacids and alpha-hydroxyacids such as pyruvic acid, alpha-hydroxyisovaleric acid and others acting as primary metabolites enabled growth of P. multocida and P. haemolytica under iron limitation.

Culture Media↗
PubMed1981

Identification of Pasteurella multocida and Pasteurella haemolytica by API 20E, Minitek, and Oxi/Ferm systems.

Fifty serotyped isolates each of Pasteurella multocida and Pasteurella haemolytica were tested on the API 20E strip (Analytab Products, Plainview, N.Y.), the Oxi/Ferm tube (Roche Diagnostics, Nutley, N.J.), and the Minitek system (BBL Microbiology Systems, Cockeysville, Md.). None of the rapid test systems reliable identified these organisms. With the API system, discrepancies between expected and actual results for the oxidase test and nitrate test frequently resulted in misidentification or no identification. The Minitek system misidentified 68% of the P. haemolytica isolates. The Minitek identification of Pasteurella depends on 100% positive xylose reactions, whereas only 56% of the P. haemolytica strains were positive for xylose fermentation. The Oxy/Ferm system, instead of giving a definitive identification, in most instances merely placed Pasteurella in a category of similar organisms.

Bacteriological Techniques↗
PubMed2003

Pasteurella multocida- and Pasteurella haemolytica-ghosts: new vaccine candidates.

Pasteurella multocida is an important animal pathogen. Bacterial ghosts produced by the expression of phage PhiX174 lysis gene E are empty cells devoid of cytoplasmic and genomic material. Lysis of P. multocida 7A and P. haemolytica A1 carrying Pasteurella-specific lysis vectors (pSR2 and pSON2) occurred 140 min after induction of gene E expression induced by temperature upshift. The E-mediated cell lysis and killing activity was the same in both Pasteurella species and no viable cells could be detected after lysis of P. multocida and P. haemolytica. Pasteurella ghosts were used for immunization of rabbits and mice. Rabbits immunized subcutaneously with either P. multocida- or P. haemolytica-ghosts developed antibodies reacting with the immunizating strain, as well as with other Pasteurella strains. The number of proteins in whole cell protein extracts recognized by the sera constantly increased during the observation period of 51 days. In addition, dose-dependent protection against homologous challenge was observed in mice immunized with P. multocida-ghosts. Animals which received 1.15 x 10(8) ghosts and a challenge dose of up to 60 cfu (LD90), showed 100% protection. According to these results, we suggest ghosts of P. multocida and P. haemolytica as new vaccine candidates.

Animals↗
PubMed1977

[Influence of Pasteurella multocida and Pasteurella-multocida endotoxin on the blood-coagulation-analytic parameter after experimental administration in calves].

Experimental administration to calves of Pasteurella multocida or the application of an endotoxin preparation of the strain produced continuous decline of thrombocytes, disorders in thrombocytic functions, and the development of hypofibrinogenaemia. The changes recorded as well as the clinical symptoms and pathologico-anatomic findings are interpreted as endotoxin shock.

Afibrinogenemia↗
PubMed1976

Multiple drug resistance in Pasteurella multocida and Pasteurella haemolytica from cattle and swine.

The results of antimicrobial susceptibility testing on 262 strains of Pasteurella multocida and 141 strains of Pasteurella haemolytica isolated from cattle and swine from 1971 to 1974 were analyzed for patterns of resistance to streptomycin, penicillin, tetracycline, and chloramphenicol, using a modified Kirby-Bauer procedure. Resistance was recorded for 80.5% of the isolants of P multocida and 92.2% of those of P haemolytica. Resistance to streptomycin was most frequent, followed by resistance to penicillin and tetracycline. Most cultures of P multocida and P haemolytica were susceptible to chloramphenicol. There were 9 patterns of resistance with the aforementioned antibiotics. The combinations, streptomycin and penicillin and streptomycin and tetracycline, each accounted for approximately 10% of the resistance patterns of P multocida. Approximately half of the 14 isolants of P haemolytica were resistant to the combination of streptomycin, penicillin, and tetracycline. These observations underscore the need for antimicrobial susceptibility testing of clinical isolants of P multocida and P haemolytica.

Animals↗
PubMed1985

Preliminary studies with a live streptomycin-dependent Pasteurella multocida and Pasteurella haemolytica vaccine for the prevention of bovine pneumonic pasteurellosis.

Twelve Pasteurella-free Holstein-Friesian calves were used in a study to test the efficacy of a live streptomycin-dependent Pasteurella multocida A:3 and streptomycin-dependent Pasteurella haemolytica A1 vaccine. The calves were inoculated intramuscularly twice at 14-day intervals with either the streptomycin-dependent vaccine, containing 1 X 10(6) colony forming units/mL P. multocida and 4 X 10(8) colony forming units/mL P. haemolytica, commercial bacterin, or phosphate buffered saline. Two weeks following the second vaccination, all calves were challenged by intranasal inoculation of 10(8) TCID50/4.0 mL infectious bovine rhinotracheitis virus followed three days later by intratracheal injection with 2.3 X 10(7) colony forming units/mL of a 16 hour culture of P. multocida A:3 and 2.6 X 10(8) colony forming units/mL of an 8 hour culture of P. haemolytica A1. Seven days after challenge with Pasteurella, calves were killed for collection of tissues at necropsy. Each calf was given a score based on macroscopic and microscopic lesions. The scores for the calves receiving live vaccines were significantly lower (p less than 0.025) than those for the controls. Also, the calves receiving live vaccines had a significant (p less than 0.05) increase in the level of serum antibody to P. haemolytica. The results of this preliminary study showed that the streptomycin-dependent vaccine offered better protection than the commercial bacterin against a virulent homologous challenge.

Animals↗
PubMed1979

[Deoxyribonucleic acids from Pasteurella multocida and Pasteurella haemolytica].

Pasteurella multocida and P. haemolytica strains contain between 1.5 and three per cent phosphorus, between nine and 14 per cent nitrogen, between two and four per cent DNA, and between five and 18 per cent RNA, the precise figures depending on culturing conditions. High-molecular DNA may be isolated by means of bacteriolysis, using deoxycholate or dodecylsulphate and the usual steps of purification, with yield and purity differing by strains. DNA with sufficient purity can be obtained from Sepharose 2 B by gel chromatography. The isolated DNA yields were characterised, base values being between 37 and 38 per cent GC for P. haemolytica and between 41 and 48 per cent GC for P. multocida. Highly suitable precursors to DNA synthesis for tritium labelling are 3H-thymidine, which is incorporated in excess of 3H-thymine by a factor of 255, as well as 3H-uracil, with its activity being recovered also from the pyrimidine bases of DNA via pyrimidine biosynthesis.

Chromatography, Gel↗