[Theoretical significance of mutation of microorganisms; consideration on publication of S.N. Muromtsev's book, Variability of microorganisms in the problem of immunity, 1953].
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In laboratory tests with carriers (glass, slab, blanket) at a high rel. humidity (95%) the survival was distinctly reduced by S. aureus at 37 degrees C and by Ps. aeruginosa at 21 degrees C and 37 degrees C. Against this after 24 h storing of contaminated carriers (glass) the resistance to disinfectants was higher at 95% rel. humidity as at 35% rel. humidity.
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A quantitative method for estimation of microbial sensitivity to doxycycline with the use of discs containing 10 gamma of the antibiotic was developed. The antibiotic concentrations in the agar were determined at a distance equal to the radius of the growth inhibition zone with the help of a curve of the dependence of the logarithm of the doxycycline concentration in agar at the period of the average critical time of the zone formation equal to 5 hours and the distance from the disc center. The antibiotic concentration in the agar at the zone edges was almost the same as the MIC of doxycycline against the test-cultures determined with the method of serial dilutions in agar.
Various microbial species, embraced by the genera, have been found to correspond to their phylogeny by the types of nutrition and the levels of pathogenicity. A relationship between the types of nutrition and the levels of parasitism and pathogenicity has been revealed. The results of analysis make it possible to assume, with a great degree of probability, the existence of new species (subspecies, variants) in the environment and/or as agents of diseases of unclear etiology.
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Five nutrient media used for determination of microbial sensitivity to antibiotics, i.e. beaf-peptone agar, Hottinger pancreatic beaf infusion agar, sprat hydrolysate nutrient agar of the Dagestan Research Institute of Nutrient Media, Muller-Chintone agar from Bulgaria and "Oxoid" agar for determination of microbial sensitivity were studied comparatively. The media were compared with respect to the growth density with the use of different test-cultures and the clearance of the inhibition growth zones around the discs containing different antibiotics. The best results were obtained with the use of sprat hydrolysate nutrient agar. Further studies on the medium standardization are necessary.
The literature data and personal observations of the authors on the effect of the inoculate amount on the results of determination of microbial sensitivity to antibiotics by the methods of serial dilutions in the liquid and solid nutrient media and agar diffusion are discussed. It was shown that the inoculate of the density of 3.6.10(7) to 4.25.10(7) microbial bodies per 1 ml was optimal for the methods of agar diffusion and serial dilutions in agar. Recommendations for simplifying standardization and dilution of the inoculate are presented.
The significance of the methods of the data interpretation for determination of the microbial sensitivity to antibiotics is discussed. A necessity for using semi-quantitative or quantitative systems of calculation instead of qualitative interpretation of the inhibition growth zone diameters is presented. It is shown that the use of the standard nutrient medium for determination of the antibiotic sensitivity in the laboratory practice may be a base for applying the methods of interpretation of the diffusion test data.