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[Multiple forms of malate dehydrogenase (EC 1.1.1.37) in Crithidia asclepii and C. oncopelti (Mastigophora, Trypanosomatidae)].

Isozyme analysis of mMDH in Crithidia asclepii and C. oncopelti by means of electrophoresis and densimetry method suggests the diploidness and genetic exchange in these organisms. Synthesis of mMDH in C. asclepii is checked by duplicated polymorphic loci MDH-1 and MDH-2, each with two alleles. In C. oncopelti two "daughter" loci are functioning too, polymorphic MDH-1 with 3 alleles and monomorphic MDG-2. Resemblance and differences in the isozyme spectra of mMDH can be used for taxonomic purposes.

Alleles↗

Effect of dietary magnesium on the susceptibility of mice to infection by protozoan parasites of the Apicomplexa and Mastigophora phyla.

Severe magnesium deficiency protects mice against infections by Plasmodium Spp. and Babesia hylomysci which invade mature erythrocytes. By contrast severe magnesium deficiency does not protect against parasite infections by P. berghei which invades reticulocytes, Toxoplasma gondii which invades macrophages, and Trypanosoma brucei which lives free in blood. The results indicate that the infectious response depends on the severity of magnesium deficiency and on the parasite species. The decrease in red blood cell magnesium and increased oxidant stress are possible explanations for the protective effect of magnesium deficiency.

Animals↗

Herbertane-type sesquiterpenoids from the liverwort Herbertus sakuraii.

Seven herbertane-type sesquiterpenoids, 1,13-dihydroxyherbertene, 1,14-dihydroxyherbertene, 1,15-dihydroxyherbertene, 12-methoxyherebertene-1,2-diol, herberteneacetal, herbertenone A and herbertenone B were isolated from the Japanese liverwort Herbertus sakuraii, together with four known herbertane- and three dimeric herbertane-type sesquiterpenoids and ent-pimara-8(14),15-dien-19-oic acid. Their structures were elucidated by spectroscopic methods. H. sakuraii is chemically similar not only to H. aduncus but also to the Mastigophora species.

Plants↗

A study of the protozoa associated with some harmful insects in the local environment.

The present study includes finding resulting from examination of three species of harmful insects, Periplaneta americana, Schistocerca gregaria and Anacanthoterms ochraceus, collected from six cities in the Central Region of Saudi Arabia. Two species of septate gregarines of the genus Gregarina Dufor, were found G. blattarum (Siebold) from mid gut of P. americana and G. rigida from gut of S. gregaria, also six species of Mastigophora in gut of white ant A. ochraceus, Trichonympha campanula, Spirotrichonympha sp., Holomastigoides hartmanni, Rostronympha magna Monocercomonas axostylis and an unidentified species. The seasonal intensity of these Protozoa and percentage of infection together with informations about the holotype materials are included. At present the holotype materials are deposited at the department of Zoology, Girls College of Education, Riyadh, Saudi Arabia.

Animals↗