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Evaluation of pH inhibition effect on activated sludge by the pseudo toxic concentration (CPT) concept model.

It is generally accepted that the inhibition effect of pH on activated sludge follows the non-competitive inhibition kinetics. But the non-competitive inhibition kinetic equation cannot be directly applied to pH inhibition, due to the difficulty in quantification of pH as a term of inhibitor concentration. So, many empirical equations were developed especially for acidic condition to describe pH inhibition effect. In this research, the pseudo toxic concentration (CPT) concept model to quantify pH inhibition effect on activated sludge was proposed and compared with other existed models. Prediction of performance, presented by Prediction Accuracy and Prediction Accuracy Index, showed that the CPT concept model can explain the reduction of the maximum specific growth rate (mu max) more accurately than any other models do at a wide range of pH. The CPT concept model was applicable not only to activated sludge but also to specific microorganism groups, such as nitrifier, less acidophilic species and nitrifying biofilm.

Bacteria↗

[Growth-inhibiting effect of psoralen plus ultraviolet-A light therapy on K562 cells].

OBJECTIVE: To observe the effects of psoralen plus ultraviolet-A light (PUVA) on K562 cells and the relative mechanism. METHODS: The effects of psoralen, ultraviolet-A light and PUVA on K562 cells were assayed by monotetrazolium test (MTT). DNA content was analyzed by flow cytometry (FCM). The apoptotic rates of K562 cells treated with 40 and 80 microg/ml psoralen for 24 and 48 hours were assayed by Annexin-V-FITC/PI reagent kit on FCM respectively. The ultrastructures of apoptotic cells were observed by a transmission electron microscope (TEM). RESULTS: Either single psoralen therapy or single ultraviolet-A irradiation had inhibiting effect on K562 cells. The inhibiting effect of PUVA on K562 cells was stronger than that of the single psoralen therapy or single ultraviolet-A light irradiation (P<0.05). Apoptotic peak (AP) was detected by FCM. TEM test showed that K562 cells treated with PUVA were smaller, having condensed cell nucleus, assembled chromatin, disintegrated nucleus body and the majority of the cells appeared to be apoptotic conformation. CONCLUSION: Psoralen has inhibiting effect on K562 cells, and the effect of PUVA is more significant. It is suggested that 10 min irradiation and 40 microg/ml terminal concentration of psoralen be probably the best choice for PUVA. The inhibiting effect of PUVA is due to apoptosis.

Apoptosis↗

Quinapril ACE-inhibition effects on adrenergic parameters in moderate essential hypertension.

The effects of inhibition of the renin-angiotensin system with quinapril on adrenergic transmission were determined in 18 patients with moderate essential hypertension. Plasmatic renin activity, catecolamines, and aldosterone as well as vascular reactivity after norepinephrine and angiotensin II perfusion were determined before and after treatment with the ACE inhibitor quinapril up to 40 mg/day during four months. In the responder patients (patients with a casual diastolic blood pressure decrease > 5 mm Hg determined at the beginning of the second vascular reactivity study, (N = 9) a decrease of plasma norepinephrine and epinephrine and a slight increase of vascular reactivity was found. Non-responder patients (N = 9) did not show any significant changes in these parameters. These results demonstrate that the hypotensive effect of quinapril may be related not only to the inhibition of RAS, but also to the blockage of the adrenergic system.

Adult↗

A meta-analysis of clinical studies on the caries-inhibiting effect of fluoride gel treatment.

A meta-analysis was performed on published data on the caries-inhibiting effect of fluoride gel treatment in 6- to 15-year-old children. The purposes of this meta-analysis were: (1) to calculate the overall caries-inhibiting effect of clinical fluoride gel treatment studies, based on explicit selection criteria, and (2) to explore factors potentially modifying the effect of fluoride gel treatment in caries prevention, concerning the baseline caries prevalence of the target population, the general fluoride regimen, and application features. The caries-inhibiting effect of fluoride gel application was assessed by the prevented fraction and the 'number needed to treat'. The overall prevented fraction of the fluoride gel treatment studies, indicating the reduction of caries incidence by fluoride gel treatment relative to the incidence in the control group, was 22% (95% CI = 18-25%). Multiple regression analysis showed no significant influence on the prevented fractions for the variables 'baseline caries prevalence', 'general fluoride regimen', 'application method', and 'application frequency'. The 'number needed to treat' (NNT), indicating the number of patients that need to be treated in order to prevent 1 DMFS, estimated the efficiency of fluoride gel treatment according to the caries incidence of the target population, including cost/effect relations. It was found that the NNT = 18 in a population with caries incidence 0.25 DMFS per year, and NNT = 3 in a population with caries incidence = 1.5 DMFS per year (treatment duration 1 year). From the standpoint of cost-effectiveness, the additional effect of fluoride gel treatment in current low and even moderate caries incidence child populations must be questioned.

Adolescent↗

The caries-inhibiting effect of a fluoride drop program: a 3-year study on Chinese kindergarten children.

OBJECTIVE: To evaluate the caries-inhibiting effect of a fluoride drops program in two kindergartens in Chengdu, Sichuan Province, a fluoride-deficient area. METHODS: For 3 years, participating children ingested in school 0.25 mg fluoride daily, in the form of drops for 2 to 3-year-olds and 0.5 mg for children age 3 years and older. These systemic fluoride supplements were made available for over 180 days each year and they were distributed by teachers who maintained attendance records. Annual examinations were conducted for 176 2-year-olds who had used the supplements in the kindergartens and 148 control children. After 3 years, 128 test and 112 control children remained in the study. RESULTS: The results demonstrated a marked reduction in dental caries in the control group: test children had 2.21 decayed, missing, or filled teeth (DMFT)-versus 4.32 in the control group, a reduction rate of 48.84%. The mean DMFT increments over the 3-year period were 1.76 and 3.92, respectively. Over a 1- to 3-year period of supplement use, the group provided with the fluoride drops had consistently lower caries prevalence than the control group. The differences were statistically significant. CONCLUSIONS: This treatment regimen demonstrated an effective caries-inhibiting effect.

Cariostatic Agents↗

A meta-analysis of clinical studies on the caries-inhibiting effect of chlorhexidine treatment.

A meta-analysis was performed on published data on the caries-inhibiting effect of chlorhexidine treatment. The results of the various studies are difficult to evaluate because of various treatment procedures, dissimilar features of participants, and different presentations of study results. A meta-analysis provides a more structured approach than the traditional review, due to systematic analysis and numerical processing of the available information. The objectives of this meta-analysis were: (1) to assess a more accurate estimate of the caries-inhibiting effect of chlorhexidine treatment than provided by individual studies, and (2) to explore factors potentially modifying the effect of chlorhexidine treatment in caries prevention, i.e., the application method, application frequency, target population, the fluoride regime, and caries criteria. Caries reduction was expressed by the prevented fraction, indicating the percentage reduction of caries incidence in the chlorhexidine group. For all prevented fractions, 95% confidence intervals were calculated. The overall caries-inhibiting effect of the chlorhexidine treatment studies was 46% (95% CI = 35% - 57%). Multiple-regression analysis showed no significant influence on the prevented fractions for the variables "application method", "application frequency", "caries risk", "fluoride regime", "caries diagnosis", and "tooth surface".

Adolescent↗

The platelet inhibiting effect of a clopidogrel bolus dose in patients on long-term acetylsalicylic acid treatment.

INTRODUCTION: Addition of clopidogrel to patients treated with ASA has been shown to decrease the incidence of in-stent thrombosis after percutaneous coronary interventions. However, it has also been reported that up to 30% of patients do not achieve adequate platelet inhibition from standard dosages of ASA and clopidogrel. There is a demand for reliable methods to measure the individual platelet inhibiting effect of this combination therapy. MATERIALS AND METHODS: The primary aim of the present investigation was to compare three methods for evaluation of the platelet inhibiting effect of a clopidogrel bolus dose in patients on long-term acetylsalicylic acid treatment. Thirty patients presenting for coronary angiography/PCI were included. Two patients were excluded due to technical problems. All patients were on 75-100 mg ASA/day for at least 8 days. Blood samples were analysed before and 16 h after a 300 mg clopidogrel bolus dose. The platelet inhibiting effect was measured with (1) Whole blood flow cytometry (17 patients); (2) a bed-side test, Platelet Mapping assay for the thrombelastograph (28 patients); and (3) PFA (Platelet function analyser) -100 (26 patients). RESULTS: With flow cytometry, the percentage of platelets expressing P-selectin (p=0.03) on their surface decreased significantly after the bolus dose of clopidogrel. There was also a reduction of platelets binding fibrinogen when stimulated with ADP. A significantly (p=0.002) increased platelet inhibition could also be demonstrated with Platelet Mapping. PFA-100 could not measure any significant platelet inhibiting effect of clopidogrel. CONCLUSION: A significant platelet inhibition could be demonstrated with flow cytometry and the Platelet Mapping assay, but not with PFA-100. However, levels of response for the individual patient with these three methods were inconsistent. Further studies are needed to evaluate how the results correlate to the clinical risk of thrombosis and bleeding.

Aged↗

[The inhibiting effect of ortofen and indomethacin in relation to the development of induced nervous system tumors in rats].

The anticarcinogenic effects of the nonsteroidal antiinflammatory drugs ortophen and indomethacin on carcinogenesis of the nervous and renal systems were studied. Glial tumors of the brain and spinal cord, neurinomas of peripheral nerves and renal mesenchymal tumors were induced in rats through a single transplacental administration of N-ethyl-N-nitrosourea, 75 mg/kg body weight. Ortophen and indomethacin each used in a dose of 20 mg/litre of drinking water in the period of postnatal life were effective in inhibiting the growth of brain and spinal cord tumors, showed a statistically insignificant tendency to suppress the growth of peripheral nervous tumors, but failed to affect the growth of renal tumors.

Animals↗

Inhibitive Effect of N-nitro-L-arginine on Hippocampal Neurons Excitation.

The inhibitive effect of N-nitro-L-arginine (L-NNA, a nitric oxide synthase inhibitor) on the excitation of cultured rat hippocampal CA1 neurons, stimulated by penicillin G(PG), was investigated. A rapid intracellular NO production induced by PG (4 000 IU/ml) was disclosed when monitored with laser scanningconfocal microscopy (LSCM). Pretreatment of L-NNA (0--10 &mgr;mol/L) dose-dependently inhibited the NO production, and the intercellular level of glutamate (15 min after PG stimulation) as well. Meanwhile, L-NNA(1 and 10 &mgr;mol/L) also significantly inhibited the immunoreactive methionine enkephalin (ir-M-ENK) level. Furthermore, the immunoreactive dynorphin B (ir-DYN-B) level was increased significantly by L-NNA (10 &mgr;mol/L). Finally, beta-FNA (100 &mgr;mol/L, an M-ENK receptor inhibitor) facilitated the inhibitive effect of L-NNA on the Glu level, while nor-BIN (100 &mgr;mol/L, a DYN receptor inhibitor) suppressed that effect. In conclusion,L-NNA could inhibit NO production induced by PG (4 000 IU/ml) stimulation, thuslowering the M-ENK level and increasing the DYN-B level, and resulted in a down-regulation of the Glu level and the neuron excitation.

Journal Article↗

Behavioural and biochemical effects of acute central metabolic inhibition: effects of acetyl-l-carnitine.

In the present study we evaluated a new method to assess the behavioural and biochemical effects of a brief period of acute hypoxia in the brain. In this method, cyanide is injected into the lateral ventricles. Spatial navigation performance in a Morris task was found to be impaired 1 and 5 min after an i.c.v. injection of 5.0 micrograms cyanide but not after 2.5 micrograms cyanide. Increased rate of phosphatidic acid formation, reflecting increased phospholipase C activity, were observed after injection of 5.0 micrograms cyanide, indicating that energy-dependent phosphoinositide metabolism was affected. Chronic treatment with acetyl-l-carnitine attenuated the cyanide-induced behavioural deficit, but had no effect on energy-dependent phosphoinositide metabolism. The results suggest that, in this model, acetyl-l-carnitine may act via free fatty acid metabolism, by increasing the reservoir of activated acyl groups which are involved in the reacylation of membrane phospholipids.

Acetylcarnitine↗

Endothelium-derived nitric oxide synthase inhibition. Effects on cerebral blood flow, pial artery diameter, and vascular morphology in rats.

BACKGROUND AND PURPOSE: We determined the effects of inhibiting the production of cerebral endothelium-derived nitric oxide on pial artery diameter, cortical blood flow, and vascular morphology. METHODS: An inhibitor of endothelium-derived nitric oxide synthesis, NG-nitro-L-arginine methyl ester hydrochloride (L-NAME), or an equivalent volume of 0.9% saline was infused into rats intra-arterially in a retrograde fashion via the right external carotid artery at a rate of 3 mg/kg/min to a total dose of 190 mg/kg or intravenously at 1 mg/kg/min to a total dose of 15 mg/kg. Large pial arteries were continuously visualized through an operating microscope, and cortical cerebral blood flow was monitored by laser-Doppler flowmetry. To localize areas of morphological interest, the protein tracer horseradish peroxidase was injected 15 minutes before termination of the L-NAME infusion and the rats were perfusion-fixed 15 minutes later for light and electron microscopic analysis. RESULTS: Infusion of L-NAME significantly raised arterial blood pressure at both doses (for 190 mg/kg, from 103.2 +/- 3.4 to 135 +/- 3.4 mm Hg; for 15 mg/kg, from 125 +/- 2.8 to 144.4 +/- 4.0 mm Hg). Pial arteries constricted within 10 minutes after the start of the intracarotid infusion to 40% of the preinfusion diameter, while cortical cerebral blood flow decreased to an average of 72.5% of that at baseline. Morphological abnormalities in the experimental rats included microvascular stasis and focal areas of blood-brain barrier disruption to protein. Ultrastructural examination of cortical leaky sites revealed constricted arterioles with many endothelial pinocytotic vesicles and microvilli. CONCLUSIONS: These observations suggest that inhibition of endothelium-derived nitric oxide synthesis affects the relation between cerebral arterial diameter and cerebral blood flow and can lead to subtle cerebral vascular pathological changes consistent with focal brain ischemia.

Amino Acid Oxidoreductases↗

[Inhibition effects of tumor infiltrating lymphocytes from oral cancer on nude mice transplanted tumor established with human tongue carcinoma cell lines].

OBJECTIVE: To observe the in vivo inhibition effects on nude mice transplanted tumor with tumor infiltrating lymphocytes (TILs) isolated from primary mass of oral cancer. METHODS: Established the transplanted tumor model of human tongue cancer at the back of BALB/C nude mice with subcutaneous injection of squamous cell carcinoma lines Tca 8113. TILs isolated from patients with Oral cancer, combining with low dose of cyclophosphamide (Cy: 50 mg/kg), were locally injected into the peripheral site of tumor. The inhibition rate (IR) was calculated by the volume of tumor mass from the 1st to the 8th week, and the weights at the 8th week after tumor dissection. RESULTS: 1. Group TIL + rIL-2 and group TIL + rIL-2 + Cy both exerted a strong inhibition effect on the transplanted tumor within three weeks. The Inhibition rate (IR) were 86.1% +/- 0.4% and 97.7% +/- 0.6% respectively at the 3rd week, while 32.1% +/- 0.3% and 80.6% +/- 0.3% at the 8th week. 2. TIL + rIL-2 + Cy expressed a stronger effect and a longer inhibition time. The IR of TIL + rIL-2 and TIL + rIL-2 + Cy were 20.0% +/- 1.4% and 75.5% +/- 2.5% respeetively at the 8th week (P < 0.01). CONCLUSION: This study demonstrated that TILs from patients with oral carcer possess strong in vivo inhibition on nude mice transplanted tumor, and low dose of cyclophasophamide can enhance the inhibition effect and prolong the inhibition time.

Animals↗

[Inhibition effects of par-4 antisense oligodeoxynucleotide on apoptosis of PC12 cell induced by glutamate is mediated by ERK1/2].

OBJECTIVE: To investigate the inhibition effects of par-4 antisense oligodeoxynucleotide on apoptosis of PC12 cell induced by glutamate and its signal transduction mechanism. METHODS: (1) Cationic lipid-mediated par-4 antisense oligodeoxynucleotide (Par-4-AS-ODN) was transfected into PC12 cells before they were treated with glutamate. Mismatch oligodeoxynucleotide (MS-ODN) were also transfected into cells as controls. (2) Morphological observation and the detection of anti-apoptosis effects of par-4-AS-ODN on PC12 cells were done with the Laser Scanning confocal Microscope by double staining the cells with acridine orange/ethidium bromide (AO/EB), addition to with flow cytometry. (3) Western blot was used to detect the protein levels of par-4 and phosphorylated ERK(1/2) at threonine-202 and Tyrosine-204. RESULTS: (1) Par-4-AS-ODN significantly suppressed up-regulation of the par-4 protein levels induced by glutamate in PC12 cells. (2) Par-4-AS-ODN could resist the decrease of phosphorylated ERK(1/2) (Thr202/Tyr204) induced by glutamate in PC12 cells. (3) Par-4 AS-ODN could inhibit apoptosis of PC12 cells induced by glutamate. But its inhibition effect could be eliminated by PD98059, a selective MEK(1) inhibitor which could inhibit phosphorylation of ERK(1/2). CONCLUSION: Par-4 AS-ODN may inhibit apoptosis of PC12 cells induced by glutamate, and its inhibition effects may be medicated by the activation of ERK(1/2).

Animals↗

Naloxone decreases the inhibiting effect of ethanol on the release of arginine-vasopressin induced by cigarette smoking in man.

In order to establish whether ethanol exerts its inhibiting effect on the nicotine-induced release of arginine-vasopressin (AVP) by interacting with an opioid pathway, six normal volunteers were treated with naloxone (2 or 4 mg as IV bolus, plus 5 or 10 mg infused over 105 minutes) during (2 nonfilter) cigarette smoking and ethanol (50 mL to 110 mL of whiskey) drinking. In addition, control experiments with naloxone, ethanol, or cigarette smoking alone were performed. When given alone, naloxone and ethanol did not modify AVP secretion, whereas nicotine increased plasma AVP levels by about 2.5-fold. This effect was completely blocked by ethanol. In the presence of naloxone, AVP rose only by about 1.7-fold in response to nicotine. Since naloxone only partially reversed the inhibiting effects of ethanol, only a partial involvement of opioid peptides in ethanol action might be supposed. Alternatively, ethanol and naloxone-sensitive opioids might produce their inhibiting effects on AVP rise in response to nicotine through independent pathways.

Adult↗

Towards a common neural substrate in the immediate and effective inhibition of stuttering.

Stuttering can be effectively inhibited via exogenous sensory signals (e.g., speaking in unison or using altered auditory feedback) or by using endogenous motoric strategies (e.g., singing or therapeutically implementing long vowel prolongations to reduce speech rates). We propose that these channels, which superficially appear to be diametrically opposite, centrally converge in the engagement of mirror neurons for fluent gestural productions. Sensory changes incurred via exogenous speech signals allow for direct engagement of mirror systems, while endogenous motor strategies appear to require significant departures from normal speech production (e.g., highly unnatural or droned speech) to engage mirror systems. Thus, paradoxically, stuttering is prone to resurface during attempts to impose naturalness upon therapeutic speech.

Feedback↗

Characterization of the inhibition effect induced by nickel on glucose-6-phosphate dehydrogenase and glutathione reductase.

Kinetic characterization of the inhibition effect of nickel on glucose-6-phosphate dehydrogenase (EC 1.1.1.49) (G-6-PD) and glutathione reductase (GR; EC 1.6.4.2) from Saccharomyces cerevisiae was made. The effect of nickel on G-6-PD activity is consistent with a mixed-type inhibition pattern, with a competitive character, since the inequality ki,int greater than ki,slope shows an inverse relation between varied substrate concentrations and fractional inhibition. An inhibition effect of nickel on GR activity, when NADPH is the varied substrate, is also consistent with a mixed-type inhibition pattern. However, pure competitive inhibition is found on GR reaction when oxidized glutathione is the varied substrate. This investigation shows the highest sensibility of GR before the inhibitory effect of nickel, in agreement with the experimental values of inhibition constants found in this study, where constants related to the GR system are lower than the ones of the G-6-PD system.

Glucosephosphate Dehydrogenase↗

The inhibiting effect of a plasma globulin on arterial thrombus formation, (as compared to dipyridamole).

The inhibiting effect on arterial white thrombus formation of a globulin prepared from beef plasma has been compared to dipyridamole in white Wistar rats. It was demonstrated that the globulin fraction had a greater effect in inhibiting thrombus formation as judged by the lag time [t(l)], the maximal thrombus value [m(T)], the maximal thickness of the thrombus [m(D)] and the maximal thrombus surface [m(O)].

Animals↗

[Morphological changes and inhibiting effect on human gastric cancer cell SGC-7901 caused by aining].

OBJECTIVE: To investigate the inhibiting effect of Aining on the human gastric cancer cells. METHODS: Morphological and MTT methods were adopted to explore the inhibiting effect of Aining and cisplatin (DDP) on the proliferation of SGC-7901 cancer cell. RESULTS: Apoptotic morphological changes were seen after the cells cultured with Aining and DDP; inhibiting rate of 1 g/L Aining group was 51%, which had no significant differences with the inhibiting rate 53% of the 25 mg/L DDP group. But both the Aining and the DDP groups were significantly different from the blank group (P < 0.01). CONCLUSION: Not only DDP but also Aining could inhibit the proliferation activity of the human gastric cancer cells, and the traditional Chinese medicine compound prescription Aining is very likely to become a new medicine which is utilized to inhibit cancer.

Adenocarcinoma↗