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Results for “Fluorescence Microscopy”

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PubMed1976

[Use of formaldehyde with pH 8 0 as a fixative for electron and fluorescence microscopy].

For fixation of the material to be investigated at the light and ultrastuctural level the author suggests to use 4% formaldehyde obtained by depolymerization of paraform on 0.1 M phosphate buffer with end pH 8.0 of the solution. This fixative stabilizes well glycogen and tissue structure. The ultrastructural characteristics of cells following continuous (up to 130 days) fixation are comparable with those obtained after fixation with aldehydes by conventional techniques. Results of histological PAS test, Feulgen's reactions and those to acid polycaccharides were identical to the results obtained in fixation with neutralized formalin. The possibility of prolonged storage of tissues makes it possible to perform delayed and repeated ultrastructural investigations of clinical and experimental material.

Animals↗
PubMed1978

Detection of androgen and estrogen receptors in human prostatic carcinoma and hyperplasia by fluorescence microscopy.

The histochemical detection of androgen and estrogen receptors in human prostatic tissue was achieved employing fluorescein labeled conjugates of bovine serum albumin linked to testosterone and estradiol. Results were compared to those of dextran-coated charcoal and protamine sulfate assays and correlated in 85% of specimens. Early findings include a relatively uniform distribution of receptor in hyperplastic epithelium in contrast to marked tumor cell receptor heterogeneity in cancer. Location of receptor in nucleus and/or cytoplasm was readily determined.

Adenocarcinoma↗