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Preliminary observations on the use of the capillary flocculation test for the diagnosis of heartwater (Cowdria ruminantium infection).

A capillary flocculation test was developed to diagnose heartwater disease of ruminants. Antigen was prepared from the brains of cattle and goats highly infected with Cowdria ruminantium. Sera were obtained from experimentally infected ruminants which either recovered naturally or with the aid of oxytetracycline treatment. Antibodies were first detected one to two weeks after clinical recovery or after treatment, and persisted for periods varying between one and four weeks. Control sera collected from cattle (sheep) and goats in the Netherlands where heartwater does not occur, or from animals serologically positive for Anaplasma marginale or Eperythrozoon ovis infections, did not react to the test.

Animals↗

Value of the Jirgl flocculation test in the diagnosis of jaundice.

The Jirgl flocculation test has been compared with the combination of the thymol reactions and serum alkaline phosphatase determination recommended by Maclagan in the investigation of 68 patients with jaundice. The test was found to be of value in distinguishing biliary obstruction from other causes of jaundice, but failed to distinguish between extrahepatic and intrahepatic biliary obstruction. Serum glutamic oxalacetic transaminase and serum glutamic pyruvic transaminase estimations were performed in a number of cases, but were found to be of no value in the diagnosis of jaundice.

Cholestasis↗

Flocculation tests.

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Flocculation Tests↗

Flocculation tests in hydatid disease.

A new serological technique of diagnosing human hydatid disease, employing polystyrene latex particles coated with hydatid cyst fluid, and a modified technique using bentonite particles are described. Comparison of the latex test with the complement-fixation test performed on 102 sera has shown a total agreement of 98% and an agreement of 93.1% in the positive specimens. The bentonite test and the complement-fixation test performed on 126 sera have shown a total agreement of 97.6% and an agreement of 90.1% in the positive specimens. Both flocculation tests are simpler to perform than the complement-fixation test. They are specific, and sensitivity closely parallels that of the complement-fixation test, with 6.9% and 6.1% of the positive specimens showing higher sensitivity in the latex and bentonite tests respectively. The bentonite test was less sensitive than the complement-fixation test in one case. None of the latex tests showed less sensitivity. The antibody active in the latex test has been demonstrated in a gamma globulin fraction of the antiserum. Some differences in the behaviour of flocculating and complement-fixing properties of sera are discussed.

Antibodies↗

The quantitative estimation of diphtheria and tetanus toxoids. 1. The flocculation test and the Lf-unit.

Considerable confusion exist in the quantitative estimation of diphtheria and tetanus toxoids. The present paper is the first in a series of papers comparing various quantitative methods. It describes the flocculation test, gives a mathematical model for the three-dimensional reaction surface, and suggests different designs which facilitate the unbiased calculation of the end-point.

Analysis of Variance↗