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Scanning electron microscopy (SEM) of some Setaria species (Filarioidea, Nematoda).

Some Setaria spp., such as S. digitata, S. marshalli, S. marshalli pandei, S. equina and S. labiatopapillosa were studied by scanning electron microscopy at the critical points in the anterior and posterior parts of the adult worms. The amphids, Deirids, find transverse striations of the cuticle and phasmidial pare, as well as the transverse bands (lugae), postdeirid and ventral papillae (only of the male) were visualized clearly at this examination. They contribute towards the identification of Setaria spp., in addition to the characteristics which are demonstrated by common light microscopy.

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[Experiments in vitro with Litomosoides carinii (Nematoda: Filarioidea). I. Maintenance of adult females and microfilariae as well as release of microfilariae in different culture media (author's transl)].

Embryos of L. carinii continue intrauterine development to microfilariae and are totally released into the medium within 5--6 days when the latter (Tc 199) is changed daily and air is used as the gas phase. Oogenesis or further fertilization of eggs, however, does not occur in vitro in any of the media examined by us. One female releases 140 X 10(3) microfilaria/day on an average in vitro within 5--6 days. Mean initial numbers of 300 X 10(3) Mf/female/day are observed. Addition of equine serum inhibits microfilarial release in vitro; normal cotton rat serum prolongs survival of females while total numbers of released microfilariae or retained embryonic stages are not increased. The serum of post-patent animals does not influence the numbers of released microfilariae or their viability or survival of females. Microfilariae released in vitro in Tc 199 + 33% normal cotton rat serum survive for more than 8 days, when air is used as the gas phase and the medium is changed daily. Microfilariae isolated from the blood of patent animals survive for at most 6 days, at a 48-hourly change of medium survival does not even exceed 4 days.

Animals↗

Vitamin A deficiency and the development of Litomosoides carinii (Nematoda, Filarioidea) in cotton rats.

This paper demonstrates that vitamin A deficient (A-) cotton rats are more susceptible to infection with Litomosoides carinii than normal (A+) cotton rats. However, embryogenesis is frequently retarded in female worms from A- hosts. Groups of young cotton rats were depleted of their liver stores of vitamin A by feeding them a vitamin A free diet from weaning. From 6 weeks of age this diet was supplemented with either 2, 5, 10, 25 or 100 IU vitamin A per 100 g body weight per day and half the animals in each group were infected with L. carinii. At autopsy, 51 days post-infection, serum vitamin A levels were proportional to vitamin A intake but were unaffected by infection (P less than 0.05). A+ cotton rats grew more rapidly and gained more weight than A- ones; infected animals gained less weight than their uninfected controls. More parasites developed in rats fed either 2 or 5 IU vitamin A than those fed either 10, 25 or 100 IU (P less than 0.02) and parasites from the 5 IU group were significantly longer than those from all other groups (P less than 0.02). However, microfilarial development was retarded in female worms from A- animals as compared with A+ ones.

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