Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Capripoxvirus”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 37 records · Page 2Linked to original sources
PubMed2003

Development of a dual recombinant vaccine to protect small ruminants against peste-des-petits-ruminants virus and capripoxvirus infections.

A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0.1 PFU protected goats against challenge with a virulent PPRV strain.

Animal Diseases↗
PubMed1994

Protection of cattle against rinderpest and lumpy skin disease with a recombinant capripoxvirus expressing the fusion protein gene of rinderpest virus.

Cattle were protected against challenge with rinderpest and lumpy skin disease viruses by vaccination with a recombinant capripoxvirus containing the fusion protein (F) gene of rinderpest virus. The minimum protective immunising doses for rinderpest and lumpy skin disease were 5.5 x 10(4) plaque forming units (pfu) and 1.5 x 10(3) pfu, respectively.

Animals↗
PubMed1987

Physical characterization of the genome of a cattle isolate of capripoxvirus.

HindIII, Pstl, Aval, and SalI site maps have been determined for the genome of a cattle isolate of capripoxvirus, KC-1. The length of the genome was estimated, by summation of the lengths of individual HindIII fragments, to be 145.6 kb, and the genome was shown to possess terminally repeated regions 1.13-6.23 kb in length. The restriction site maps demonstrate that the genome of KC-1 does not possess a high degree of nucleotide sequence homology with the genomes of isolates of orthopoxvirus, parapoxvirus, leporipoxvirus, or African swine fever virus.

Chromosome Mapping↗
PubMed1989

A capripoxvirus pseudogene whose only intact homologs are in other poxvirus genomes.

Equivalent regions from within the inverted terminal repeats (ITRs) of the genomes of two capripoxviruses, KS-1 and InS-1, were sequenced. The sequence from KS-1 DNA covers the major part of three contiguous open reading frames (ORFs), which match three contiguous ORFs from within the genomic ITRs of the leporipoxvirus Shope Fibroma Virus (SFV). The sequenced region of InS-1 DNA contains only two of the three ORFs. The region homologous to the third ORF has no coding potential due to the presence of several stop codons, resulting from small frameshifting deletions and insertions. The significance of a degenerate poxvirus gene, intact homologs of which are only found in other poxvirus genomes, is discussed.

Amino Acid Sequence↗
PubMed1993

Single capripoxvirus recombinant vaccine for the protection of cattle against rinderpest and lumpy skin disease.

A recombinant capripoxvirus has been constructed containing a full-length cDNA of the fusion protein gene of rinderpest virus. The gene was inserted in the thymidine kinase gene of the capripox genome under the control of the vaccinia virus major late promoter p11 together with the Escherichia coli gpt gene in the opposite orientation under the control of the vaccinia early/late promoter p7.5. A vaccine prepared from this recombinant virus protected cattle against clinical rinderpest after a lethal challenge with a virulent virus isolate. In addition, the vaccine protected the cattle against lumpy skin disease.

Animals↗
PubMed1986

Studies on the major common precipitating antigen of capripoxvirus.

The proteins of sheep pox, goat pox, sheep and goat pox and lumpy skin disease (Neethling) viruses were labelled with [35S]methionine. The major structural polypeptides of these viruses co-migrated on polyacrylamide gels, demonstrating the very close biochemical relationship between them. Using the agar gel immunodiffusion (AGID) test with radiolabelled antigen preparations, a major common precipitating antigen was identified. This co-migrated on polyacrylamide gels with one of the major structural polypeptides [mol. wt. 67000 (67K)]. The use of [35S]methionine-labelled antigen preparations considerably improved the sensitivity of the AGID test as a diagnostic test for capripoxvirus antibody detection.

Animals↗
PubMed1986

Passive protection of sheep against capripoxvirus.

The close antigenic relationship between strains of capripox was shown by passively immunising sheep with serum against capripoxviruses isolated from a sheep and from a goat. Sheep immunised with immune serum to Oman sheep pox or Yemen goat pox resisted challenge with Yemen goat pox or Nigeria sheep pox respectively. Lambs born to sheep previously infected with isolates of capripox from the Sudan, India and Nigeria were also protected against challenge with Yemen goat pox.

Animals↗
PubMed1986

Comparison of the external dimensions of capripoxvirus isolates.

No significant difference was found between the external dimensions of the M forms of isolates of sheep pox virus from Nigeria, sheep and goat pox virus from Kenya and lumpy skin disease virus from South Africa. Earlier reports that isolates of capripoxvirus can be distinguished by their relative sizes could not be substantiated.

Animals↗
PubMed2006

A sheeppox virus outbreak in Central Turkey in 2003: isolation and identification of capripoxvirus ovis.

Poxvirus epidemics occur almost every year and cause significant economic losses for small-scale animal producers in Turkey. In this study, the causative agent of the most recent epidemic in Central Anatolia was detected in clinical samples using electron microscopy (EM) and amplified using an in house polymerase chain reaction procedure for the first time. Additionally, the aetiological agent was isolated from a sheep and identified using EM and PCR.

Animals↗
PubMed1999

Identification and characterisation of an early/late bi-directional promoter of the capripoxvirus, lumpy skin disease virus.

Identification and characterisation of an early/late bi-directional promoter element of lumpy skin disease virus (LSDV) is described. The 56 bp element shows substantial structural similarities with other poxvirus promoters, providing further evidence that transcriptional elements are conserved within the Poxviridae. The relative strengths of the LSDV early and late promoters were compared to the vaccinia virus (VV) P7.5K and P11K promoters in transient expression assays. These transient assays also verified the temporally regulated nature of the promoter. Favourable expression levels suggest that the identified LSDV promoters could be used for expressing foreign genes in a recombinant LSDV system.

Amino Acid Sequence↗
PubMed1997

Trial of a capripoxvirus-rinderpest recombinant vaccine in African cattle.

Cattle were vaccinated with differing doses of an equal mixture of capripox-rinderpest recombinant viruses expressing either the fusion protein (F) or the haemagglutinin protein (H) of rinderpest virus. Animals vaccinated with 2 x 10(4) p.f.u. or greater of the combined viruses were completely protected against challenge, 1 month later, with both virulent rinderpest and lumpy skin disease viruses. Vaccination with any of the doses did not induce any adverse clinical response in the animals or transmission of the vaccine virus between animals. All cattle challenged 6 or 12 months after vaccination with 2 x 10(5) p.f.u. of the mixture of recombinant viruses were protected from severe rinderpest disease. Ten out of 18 were completely protected while the remaining 8 developed mild clinical signs of rinderpest. Cattle vaccinated with the recombinant vaccines after prior infection with the parental capripox virus showed more marked clinical signs of rinderpest after challenge with virulent rinderpest, but 9 out of 10 recovered, compared with 80% mortality in the unvaccinated controls.

Animals↗
PubMed2000

Detection of sheep poxvirus in skin biopsy samples by a multiplex polymerase chain reaction.

The development of a multiplex polymerase chain reaction (PCR) method with amplification of capripoxvirus in a single-step procedure from skin biopsies using three primer pairs, two specific for capripoxvirus and one specific for alpha-tubulin is described. A sensitive multiplex PCR was achieved by optimization of parameters such as the primer concentrations, magnesium and dNTPs concentrations. False negative results that sometimes arise due to inhibitors of DNA amplification may be avoided by the inclusion in the assay of alpha-tubulin primers. The results reported on 42 skin biopsies from sheep suspected to have poxvirus infection, indicated that the assay could monitor simultaneously DNA extraction from skin biopsy samples and allow improved detection of capripoxvirus within 24 h of specimen receipt in the laboratory.

Animals↗
PubMed2004

Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of P32 gene.

Sheep pox and Goat pox are highly contagious viral diseases of small ruminants. These diseases were earlier thought to be caused by a single species of virus, as they are serologically indistinguishable. P32, one of the major immunogenic genes of Capripoxvirus, was isolated and Sequenced from two Indian isolates of goat poxvirus (GPV) and a vaccine strain of sheep poxvirus (SPV). The sequences were compared with other P32 sequences of capripoxviruses available in the database. Sequence analysis revealed that sheep pox and goat poxviruses share 97.5 and 94.7% homology at nucleotide and amino acid level, respectively. A major difference between them is the presence of an additional aspartic acid at 55th position of P32 of sheep poxvirus that is absent in both goat poxvirus and lumpy skin disease virus. Further, six unique neutral nucleotide substitutions were observed at positions 77, 275, 403, 552, 867 and 964 in the sequence of goat poxvirus, which can be taken as GPV signature residues. Similar unique nucleotide signatures could be identified in SPV and LSDV sequences also. Phylogenetic analysis showed that members of the Capripoxvirus could be delineated into three distinct clusters of GPV, SPV and LSDV based on the P32 genomic sequence. Using this information, a PCR-RFLP method has been developed for unequivocal genomic differentiation of SPV and GPV.

Amino Acid Sequence↗
PubMed2002

The genomes of sheeppox and goatpox viruses.

Sheeppox virus (SPPV) and goatpox virus (GTPV), members of the Capripoxvirus genus of the Poxviridae, are etiologic agents of important diseases of sheep and goats in northern and central Africa, southwest and central Asia, and the Indian subcontinent. Here we report the genomic sequence and comparative analysis of five SPPV and GTPV isolates, including three pathogenic field isolates and two attenuated vaccine viruses. SPPV and GTPV genomes are approximately 150 kbp and are strikingly similar to each other, exhibiting 96% nucleotide identity over their entire length. Wild-type genomes share at least 147 putative genes, including conserved poxvirus replicative and structural genes and genes likely involved in virulence and host range. SPPV and GTPV genomes are very similar to that of lumpy skin disease virus (LSDV), sharing 97% nucleotide identity. All SPPV and GTPV genes are present in LSDV. Notably in both SPPV and GTPV genomes, nine LSDV genes with likely virulence and host range functions are disrupted, including a gene unique to LSDV (LSDV132) and genes similar to those coding for interleukin-1 receptor, myxoma virus M003.2 and M004.1 genes (two copies each), and vaccinia virus F11L, N2L, and K7L genes. The absence of these genes in SPPV and GTPV suggests a significant role for them in the bovine host range. SPPV and GTPV genomes contain specific nucleotide differences, suggesting they are phylogenetically distinct. Relatively few genomic changes in SPPV and GTPV vaccine viruses account for viral attenuation, because they contain 71 and 7 genomic changes compared to their respective field strains. Notable genetic changes include mutation or disruption of genes with predicted functions involving virulence and host range, including two ankyrin repeat proteins in SPPV and three kelch-like proteins in GTPV. These comparative genomic data indicate the close genetic relationship among capripoxviruses, and they suggest that SPPV and GTPV are distinct and likely derived from an LSDV-like ancestor.

Animals↗
PubMed2003

Vaccines for lumpy skin disease, sheep pox and goat pox.

Sheep pox, goat pox and lumpy skin disease (Neethling) are diseases of sheep, goats and cattle respectively, caused by strains of poxvirus, within the genus Capripoxvirus. Strains affecting sheep and goats are not totally host-specific; some cause disease in both sheep and goats while others may cause disease in only one species. Those causing disease in cattle appear to be specific for cattle, and this is reflected in the different geographical distribution of lumpy skin disease (LSD) and sheep pox and goat pox (sheep and goat pox); LSD is confined to Africa, while sheep and goat pox are present in Africa north of the equator, and throughout West Asia and India, as far East as China and Bangladesh. Occasionally sheep and goat pox spreads from Turkey into Greece. All strains of capripoxvirus so far examined are antigenically indistinguishable, and recovery from infection with one strain provides immunity against all other strains. Because of this antigenic homology among all strains, there is the potential to use a single vaccine strain to protect cattle, sheep and goats.

Animals↗
PubMed1991

Identification and nucleotide sequence of the thymidine kinase gene of swinepox virus.

Using degenerative oligonucleotide probes, representing two different conserved regions of poxvirus and mammalian thymidine kinase (TK) genes, the swinepox virus (SPV) TK gene was mapped to a 1.7-kb BamHI-HindIII fragment of the viral genome. Nucleotide sequencing of this DNA piece revealed that the SPV TK gene was encoded by an open reading frame (ORF) of 177 codons. Immediately downstream of the TK gene was a second ORF with homologues at the same location in both capripoxvirus and leporipoxvirus genomes. A similar gene had translocated to near the left hand terminus of the vaccinia virus (orthopoxvirus) genome. Flanking the two SPV genes were ORFs whose counterparts in other poxvirus genera are located at the same relative positions. SPV appeared to be most closely related to capripoxvirus, based on the organization of the four genes and on the percentage of identical amino acid residues of the respective encoded proteins.

Amino Acid Sequence↗