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Ultrasound as an approach to assessing body composition.

Body composition is an important indicator of nutritional status. The most commonly used indirect method for estimating body fat is based on measurements of subcutaneous fat tissue. It has been suggested that ultrasonic measurements may be more precise than those of the caliper and therefore may yield more accurate measures of subcutaneous fat tissue. This study was designed to correlate ultrasonic and caliper measurements of subcutaneous fat with body density determined by hydrostatic weighing. Subcutaneous fat thickness was measured at seven body sites (triceps, biceps, subscapula, waist, suprailiac, thigh, and calf) with a Lange skinfold caliper and an ADR ultrasonic scanner, equipped with a display-screen, 7MHz transducer, and electronic calipers. Regression equations to predict body density, and hence body fat, were derived for each technique using a minimal number of body sites. The sample consisted of 124 white men, aged 18 to 30 yr. Mean body density determined by hydrostatic weighing was 1.07 g/ml (SD +/- 0.01) and mean body fat was 12.7% (SD +/- 5.8). Both ultrasonic and caliper measurements of waist, thigh, and triceps had the highest correlation with body density. Regression equations using these three sites in all possible two-site combinations were derived for each technique. The predictions of body density from these equations did not differ significantly. These results suggest that in free-living, nonobese, white men, body fat can be estimated with nearly the same degree of accuracy using either the caliper or ultrasonic technique.

Adipose Tissue↗

Failure to demonstrate an effect of dietary fatty acid composition on body weight, body composition and parameters of lipid metabolism in mature rats.

The objectives of the present study were to examine the effects of dietary fatty acid composition on body composition and on several parameters of lipid metabolism in adipose tissue and to assess the ease of weight loss with restricted dietary intake. Rats were fed diets containing 14% fish oil, safflower oil or beef fat plus 2% corn oil. These diets were fed ad libitum for 4 wk (first phase). A number of the animals from each group were killed, and the others were fed 50% of their first-phase food intake for an additional 4 wk (second phase). The diets used in the second phase contained 3% of the above fats plus 2% corn oil. Food consumption, food efficiency and body weights were monitored. After the rats were killed, fatty acid composition of epididymal fat pads was examined. In addition, in vivo lipolysis and in vitro lipogenesis in epididymal fat pads were examined. The results indicate that dietary fatty acid composition had no effect on body weight, food consumption, in vivo lipolysis and in vitro lipogenesis in epididymal fat pads. In addition, although dietary fatty acid manipulation resulted in alteration in adipose tissue fatty acid composition, it had no effect on the rate of weight loss, body composition, in vivo lipolysis and in vitro lipogenesis in epididymal fat pads. It is concluded that dietary fatty acid composition does not play a role in body composition and in lipid metabolism in adipose tissue of mature rats.

Animals↗

Postmenopausal weight status, body composition and body fat distribution in relation to parameters of menstrual and reproductive history.

OBJECTIVES: In the present study the association between menstrual and reproductive history patterns and weight status, fat distribution and body composition during postmenopause was tested. METHODS: In 106 healthy postmenopausal women ranging in age from 48 to 58 years (x = 53.7 year) the weight status was classified according to the recommendations of the WHO. Additionally body composition was estimated by dual energy X-ray absorptiometry and fat distribution was calculated using the fat distribution index. Weight status, body composition and fat distribution were correlated with self-reported parameters of menstrual and reproductive history (age at menarche, average cycle length, number of births, age at first and last birth, average pregnancy weight gain, age at menopause). RESULTS: It was shown that number of births, age at first birth and pregnancy weight gain were related significantly to the postmenopausal weight status, body composition and fat distribution. CONCLUSION: An early first birth a low number of births and a high weight gain during pregnancies can be assumed as risk factors for overweight, a higher amount of adipose tissue, android fat patterning and therefore for the development of the metabolic syndrome during postmenopause. In contrast no adverse effect of menstrual and reproductive parameters on postmenopausal bone mass was found.

Adipose Tissue↗

Association between body composition and body mass index in young Japanese women.

The National Nutrition Survey of Japan indicated a trend toward a decreasing body mass index (BMI; kg/m2) among young Japanese women. Current studies suggest that not-high BMI often does not correlate with not-high body fat percentage. Recently, the classification of BMI in adult Asians was proposed by the International Obesity Task Force. The addition of an "at risk of overweight" category, BMI as 23.0-24.9, was intended to prevent chronic diseases. We investigated the association between body fat percentage (BF%) and BMI to evaluate the screening performance of BMI focused on individual preventive medicine. The subjects consisted of 605 female college students. The subjects' ages (y), heights (cm), body weights (kg), BMIs, and BF percents with underwater weighing expressed as the means +/- SD were 19.6 +/- 0.5, 158.7 +/- 5.6, 53.8 +/- 7.2, 21.3 +/- 2.4, and 24.9 +/- 4.9, respectively. We defined high BF% as +/- 85th percentile of BF% (29.8%). High-BF% individuals are often not classified into BMI > or = 23.0 because their BMI readings are very broad (18.4-31.7). In comparison to the screening performances (specificity and sensitivity), BMI > or = 23.0 (85.3% and 52.1%, respectively), rather than BMI > or = 25.0 (96.7% and 29.8%, respectively), is recommended for the mass evaluation of fatness. For this reason, the BMI "at risk of overweight" category is characterized as the threshold of increasing the appearance ratio of high-BF% individuals. In conclusion, the BMI > or = 25.0 kg/m2 category is determined as high BF%, regardless of body composition measurement for mass evaluation as a result of quite high specificity. Even so, body composition measurement is necessitated by the individual evaluation of fatness focused on preventive medicine because BMI performed a poor representation of body composition, especially BMI < 25.0 kg/m2 individuals.

Adipose Tissue↗

Diet composition and body composition in preschool children.

BACKGROUND: In studies of adult humans and in animal models, dietary intakes of the macronutrients, particularly fat, are related to body composition; however, data on children are more scarce. OBJECTIVE: We sought to determine whether diet composition is related to percentage body fat in children aged 1.5-4.5 y. DESIGN: In 77 preschool children, a 4-d weighed-food record was used to determine intakes of total energy and energy from each macronutrient. An oxygen-18 dilution method was used to calculate percentage body fat. Habitual physical activity level was determined by calculating the ratio of total energy expenditure (from stable isotope analyses) to predicted basal metabolic rate. Dietary intake and body-composition data were analyzed to evaluate whether diet composition was related to body fat. Further analyses incorporating physical activity level were performed. RESULTS: Percentage body fat was not significantly correlated with dietary intake variables (total energy or percentage of energy from fat, carbohydrate, or protein) and did not differ significantly among 3 increasing levels of each dietary intake variable by analysis of variance. In multiple regression analysis, physical activity level was related to body fat whereas diet composition was not. CONCLUSIONS: We found no relations between dietary intakes of total energy, fat, carbohydrate, or protein and percentage body fat in children. The relation between fat intake and body fat may develop over time and may not be evident in preschool children. Energy expenditure, in particular physical activity level, may have a greater influence on body composition in early childhood.

Adipose Tissue↗

Body composition and body weight: criteria of overnutrition.

The terms "body weight" and "body composition" are by no means synonymous, and attention is increasingly being focused on body composition. A measurement of relative fatness is a better criterion of caloric overnutrition than is body weight.The simple technique of skinfold measurement using established standard methods is the most practical for use in the field to obtain an estimate of fatness or caloric overnutrition. The current need is for the establishment of "norms" for skinfolds for population groups of all ages and both sexes. When these are established, excellent simple criteria for overnutrition will be available.Prior to the establishment of norms, more work is needed to indicate which skinfolds for each age group and for each sex best reflect total body fatness. Body fatness may then be studied in relation to body weight and both may be related to morbidity, mortality and longevity. Finally, the answer may be obtained to the question whether it is overweight per se or overfatness that is related to excess morbidity and mortality.

Adolescent↗

Effect of percutaneous androgen replacement therapy on body composition and body weight in postmenopausal women.

OBJECTIVES: This study was carried out to assess the effect of topical androgen replacement therapy on body weight, body composition and fat distribution in postmenopausal women. METHODS: 39 healthy postmenopausal women (51.4 +/- 2.24 years), with increasing body weight, were prospectively studied for 6 months. Body composition (fat mass, kg, %) was measured by means of dual-energy X-ray absorptiometry (DXA). Hormonal and lipid parameters were also measured. Subjects were divided into two groups. An androgen gel (group A) or placebo gel (group P) was topically administered to the abdominal and gluteo-femoral regions. DXA was performed before commencement of topical treatment and after 6 months. RESULTS: A highly significant total body weight reduction was found in group A (68.0 +/- 13.1 to 65.4 +/- 11.8 kg). Abdominal fat (37.3 +/- 11.2 to 35.1 +/- 9.7%), gluteo-femoral fat (46.3 +/- 6.6 to 45.4 +/- 7.7%), total body fat (38.2 +/- 7.9 to 36.1 +/- 8.6%) and BMI (24.8 +/- 4.3 to 23.7 +/- 3.8) were also found to have decreased significantly in this group. No significant reduction in body weight (kg) and body fat (%) could be measured in the placebo group. No influence on lipid parameters was found although total testosterone increased significantly in group A (0.29 +/- 0.24 to 0.72 +/- 0.17 ng/ml). CONCLUSIONS: Topically applied androgen is capable of reducing abdominal fat accumulations as well as total body weight in postmenopausal women with unexplained weight gain. In contrast to systemic androgen application, topical administration has no effect on the lipid profile. Gluteal fat, however, is less effectively influenced by androgens.

Body Composition↗

Body composition and body hydration in old age estimated by means of skinfold thickness and deuterium dilution.

In this study a group of 30 women and 30 men aged 60-90 years was examined. Body density was predicted from the sum of four skinfold thickness (SFT) as described by Durnin and Womersley (1974). Body composition, expressed as a percentage of total body weight as fat mass (FM) and fat free mass (FFM) was calculated by means of Siri's formula (Siri 1961). Total body water (TBW) was measured from the dilution of an oral deuterium dose and FFM calculated assuming a hydration of 73.2%. The FM% predicted from SFT showed a slight but not significant decreasing age trend from 28.3% to 25.4% in men, and from 40.3% to 37.8% in women. Body hydration (about 50% of body weight in men and 44% in women) remained constant throughout the age range. FFM obtained from TBW assuming a constant hydration of FFM, was found to be unchanged with age. FM% predicted from SFT was correlated with values obtained from TBW on an individual basis (R2 = 0.38; p less than 0.0001 in men and R2 = 0.31; p less than 0.002 in women), but the scatter was large. The Bland and Altman statistical analysis (Bland and Altman 1986) suggests that the discrepancy between the two estimates tends to be significantly more positive with increasing fatness of the subject. The assumption of a constant hydration of FFM between individuals and with advancing age was tested. The hydration of FFM was calculated as the ratio between TBW (from deuterium dilution) and FFM (estimated from SFT). The wide range of values (50% to 90%) suggests large inter-individual differences. The mean values were lower than commonly reported, and differed with age and sex. In men, FFM hydration steadily decreases with age from the 7th decade (70.2 +/- 7.7%) to the 10th decade (65.9 +/- 8.2%). Women showed higher average figures than those of men and a lower age-associated decline (72.5% and 68.5% respectively). Our results suggest that FFM hydration decreases with age, and this indicates the need to develop age-related correction factors in order to derive FFM from TBW in an elderly population.

Aged↗

Reproducibility of body composition and body water spaces measurements in healthy elderly individuals.

BACKGROUND AND AIMS: The assessment of body composition and water spaces in elderly persons, especially in disease situations, would be a critical component of the nutritional and hydration management. Bioelectrical impedance analysis (BIA) is a validated bed-side technique for the estimate of total body (TBW) and extracellular (ECW) water. The aim of the present study was to address the reproducibility of estimates of TBW, ECW and fat-free mass (FFM) in healthy elderly individuals (n=24, 61 +/- 3.6 yrs). METHODS: Repeated measurements of weight, height, skinfold thickness, TBW and ECW (BIA) were obtained (in almost identical experimental conditions) on days 0, 14, 28 and 42. FFM and the hydration fraction of FFM (HF) were calculated with a three compartment model. RESULTS: Analysis of variance for repeated measurements showed that time did not explain a significant part of the variance in water spaces, FFM, and HF. Mean SDs for repeated measurements were well within 1 litre for water spaces, 1 kg for FFM and 1% for HF. CONCLUSIONS: We conclude that reproducible measurements of water spaces can be obtained with BIA, which could be used to monitor hydration changes. Given the small variability in HF, FFM, can be derived from BIA with good reproducibility.

Aging↗

ICV beta-hydroxybutyrate: effects on food intake, body composition, and body weight in rats.

This study examined the effect of long-term intracerebroventricular (ICV) infusion of beta-hydroxybutyrate (beta HB) on food intake, diet selection, body weight, and body composition in rats. Female rats were divided into 2 groups and implanted with a 28-day osmotic pump connected to a lateral cerebroventricular cannula. One group was infused with artificial cerebrospinal fluid (aCSF) and the other with beta HB for 28 days. The rats had free access to both a high-fat/low-carbohydrate and a low-fat/high-carbohydrate diet (isocaloric) for the 28-day infusion period. The group infused with beta HB had a significantly lower body weight gain during the infusion period. Cumulative food intake increased in the same manner in both groups. Fat pad weights and carcass lipid content were significantly higher in beta HB rats, despite the equivalent caloric intake in both groups and the decreased body weight. Our observations are in accord with earlier studies indicating that beta HB infused ICV reduces body weight, but not necessarily food intake. Increased adiposity in association with decreased body weight change in beta HB-infused rats strongly suggests that energy is being partitioned to fat deposition at the expense of lean tissue growth when ketone bodies are infused into the cerebroventricles.

3-Hydroxybutyric Acid↗

Familial and environmental influences on body composition and body fat distribution in childhood in southern Italy.

The aim of this paper was to evaluate the factors affecting body fat excess and distribution in prepubertal age. A cross-sectional survey was carried out on children attending the 4th grade of a primary school in Naples. Eighty-eight per cent of the total sample was examined: 52 girls, 58 boys; mean age = 9.6 yrs (s.e. +/- 0.10). Each child underwent a medical examination, anthropometric measurements and bio-impedance analysis of body composition. The parents were asked to fill in a questionnaire that included demographic data, family history, parent's weight and height, child's perinatal history and his or her involvement in sports activities. Data were analyzed by multiple linear regression. The results showed a direct correlation between parental BMI and children's anthropometric measurements: the children's BMI correlated with the fathers' (P = 0.02) and mothers' BMI (P = 0.027); the children's waist/hip ratio correlated with the fathers' BMI (P = 0.07); the children's subscapular skinfolds correlated with the father's (P = 0.07) and mothers' BMI (P = 0.02); the children's triceps skinfolds correlated with the fathers' BMI (P = 0.004). Among congenital factors, sex was shown to be correlated with the children's waist/hip ratio (P = 0.05) with a lower ratio in the female, indicating a sex influence on body fat distribution even in prepubertal age. The children's BMI correlated with their waist/hip ratio (P = 0.001). Children's systolic blood pressure showed a positive correlation with triceps (P = 0.04) and subscapular (P = 0.05) skinfolds thickness % FAT-PLI (P = 0.02).(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗

Assessing body composition and changes in body composition. Another look at dual-energy X-ray absorptiometry.

Dual-energy X-ray absorptiometry (DXA) is selected with increasing frequency as a method for both assessing body composition and measuring the changes in body composition. Issues have been raised about hydration, software version, hardware (fan beam vs. pencil beam), and the subject population in relation to the validity of DXA-derived estimates of body composition. This paper reviews validation studies of DXA to assess the impact of recent developments in its technology. Studies by Prior et al., Kohrt et al., Salamone et al., Going et al., and Pietrobelli et al. demonstrate the effectiveness of DXA estimates of changes in body composition. By contrast, Clasey et al., Nelson et al., and Friedl et al. found limitations in DXA estimates of body composition and its changes. These contradictory conclusions were explored for threats to internal validity in each research study. From this analysis, two validation guidelines are recommended for use when evaluating estimates of body composition. When multicomponent models are used, it is essential that estimates of body water as a fraction of fat-free mass fall in the expected range (71 to 75%) and have a relatively small standard deviation (2 to 3%). For measuring changes in body composition, DXA estimates of total body mass must accurately reflect both baseline and posttreatment scale body weight estimates. Failure to meet these guidelines threatens the internal validity of the study and raises the likelihood of methodological discrepancies. Applying these criteria to DXA studies of body composition under review accounts for much of the contradictory conclusions among investigations.

Absorptiometry, Photon↗

Android obesity at diagnosis and breast carcinoma survival: Evaluation of the effects of anthropometric variables at diagnosis, including body composition and body fat distribution and weight gain during life span,and survival from breast carcinoma.

BACKGROUND: Although a large body of research exists concerning pathologic prognostic indicators of the rate of incidence and survival from breast carcinoma, to the authors' knowledge very few studies have examined the effects of anthropometric variables such as height, obesity, weight gain in adulthood, timing of weight gain, and body composition to survival, although these variables are related to the incidence rate. METHODS: The survival status of 166 patients diagnosed with primary breast carcinoma and followed for at least 10 years was obtained from the Cancer Center's registry, and significant anthropometric and other known prognostic indicators regarding survival after diagnosis were determined by Cox proportional hazards analysis. RESULTS: Eighty-three of 166 breast carcinoma patients (50%) with up to 10 years of follow-up died of disease. Android body fat distribution, as indicated by a higher suprailiac:thigh ratio, was a statistically significant (P < 0.0001) prognostic indicator for survival after controlling for stage of disease, with a hazards ratio of 2.6 (95% confidence interval [95% CI], 1.63-4.17). Adult weight gain, as indicated specifically by weight at age 30 years, was a statistically significant (P < 0.05) prognostic indicator for survival with a hazards ratio of 1.15 (95% CI, 1.0-1.28). In addition, the authors observed the Quatelet Index, a negatively significant (P < 0.01) prognostic indicator for survival with a hazards ratio of 0.92 (95% CI, 0.87-0.98). Other markers of general obesity such as weight at diagnosis, percent body fat, and body surface area were not significant markers influencing survival. Similarly, height; triceps, biceps; subscapular, suprailiac, abdominal, and thigh skinfolds; waist and hip circumferences; family history; and reproductive and hormonal variables at the time of diagnosis showed no apparent significant relation to survival. CONCLUSIONS: The results of the current study provide some evidence that android body fat distribution at diagnosis and increased weight at age 30 years increases a woman's risk of dying of breast carcinoma.

Adult↗