Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “BETA GLOBULIN”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 37 records · Page 2Linked to original sources
PubMed1976

[Comparative immunochemical analysis of specific beta globulins of the "pregnancy zone" of the humans and other mammals].

By means of the immunodiffusion method it was shown that human pregnancy-specific beta-globulin, as well as analogous beta-globulins in the pregnancy serum of mammal animals (rat, guinea-pig) had an individual antigenic structure. During pregnancy the blood serum of the allied species (rat, mouse) contained immunologically similar beta-globulins which apparently were synthesized in the placenta.

Animals↗
PubMed2004

Identification and characterization of linear B-cell epitopes of beta-globulin, a major allergen of sesame seeds.

BACKGROUND: The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions. OBJECTIVE: This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd beta-globulin, the major allergen of sesame seed. METHODS: A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on beta-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame beta-globulin served to identify the epitopes by using the dot-blot test. RESULTS: At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the beta-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of beta-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues. CONCLUSION: Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy.

Adolescent↗
PubMed1984

Polymorphism and inheritance of serum esterases and beta-globulins in the paradise fish (Macropodus opercularis; Anabantidae).

Electrophoretic variants of serum esterases and beta-globulins in two subspecies of paradise fish (Macropodus opercularis) were studied. Four esterase loci (Est-1, Est-2, Est-3 and Est-4), a single transferrin (Tf) and another major beta-globulin locus (Bg) were identified by segregational analysis. Est-3 seems to be a monomorphic locus. Three alleles of Est-1, two of Est-2, two of Est-4, four of Tf and two alleles of Bg were found in the laboratory population. None of these loci were closely linked. Electrophoretic patterns of F1 hybrids confirmed the monomeric structures of each of the studied proteins. Allelic segregation at the Tf and Bg loci was normal in F2 and backcross populations. In crosses of the two Macropodus subspecies there were deviations from Mendelian ratios because of missing recombinant esterase phenotypes. Each of these would have been homozygous Est-2f/f. We suppose that Est-2f/f causes lethality in the early phase of development, except in the Est-1c/c, Est-2f/f combination characteristic of the parental subspecies M.o. concolor.

Alleles↗
PubMed1980

[Organ-specific human prostate beta-globulin during tumor growth].

An organ specific protein antigen having an electrophoretic mobility of beta-globulin was demonstrated in human prostate. Its physicochemical properties (relative electrophoretic mobility, diffusion coefficient, molecular weight and relation to different precipitating agents) were determined. The indirect immunofluorescence technique revealed that this antigen is synthesized by the glandular epithelium of the main glands. A statistically significant decrease was found in the concentration of prostatic beta-globulin in tumors of the prostate. The synthesis of this protein was shown to start only in the period of puberty.

Antigens↗
PubMed1980

Human beta-globulin factors affecting growth of human lymphocytes and mouse fibroblasts.

Evidence is presented to show the existence in human beta-globulin (Cohn fraction III) of 2 growth-promoting factors that stimulate DNA synthesis and cell division in human lymphocytes and 3T3 B mouse fibroblasts. After extraction of human beta-globulins at pH 3.0 in 0.1 M NaCl followed by sieve chromatography on Sephadex, 2 distinct fractions were obtained containing the biological activity; one of polypeptide nature and molecular weight of approx. 10 000 Daltons and the other consisting of a homogeneous ribonucleic acid, as revealed by polyacrylamide gel electrophoresis. The above-mentioned factors appear to lack both cell and species specificity, since they are mitogenic agents for cells as diverse as mouse fibroblasts and human lymphocytes.

Animals↗
PubMed1993

Interaction of myo-inositol hexaphosphate (MIHP) with beta-globulin from Sesamum indicum L. Kinetics and thermodynamics.

beta-Globulin, the low molecular weight protein fraction from Sesamum indicum L., interacts with myo-inositol hexaphosphate (MIHP) maximally at pH 3.0, with concomitant precipitation up to 85 +/- 2% at an MIHP concentration of 8 x 10(-4) M. The kinetics of interaction as followed by stopped-flow spectrophotometry suggested the reaction to be of pseudo first-order, having an initial fast step followed by a relatively slow step of rate constants 1.9 x 10(-2) s-1 and 1.2 x 10(-3) s-1, respectively at 1 x 10(-4) M MIHP concentration. The analysis of the complex indicated the presence of polymer as seen in sedimentation velocity experiment. This was accompanied by conformational change of a three-fold decrease in beta-structure and also an increase in fluorescence emission intensity accompanied with a red shift from 330 to 334 nm. Stoichiometric analysis of MIHP binding suggested four independent binding sites for MIHP, with a free energy change, delta G zero = 5.1 kcal mol-1 resulting from a binding constant of 3.6 x 10(3) M-1.

Beta-Globulins↗
PubMed1981

[Evaluation of a trophoblastic beta-globulin preparation in the leukocyte adhesion inhibition test during pregnancy].

The leukocyte adhesion inhibition test was used to study the effect of trophoblastic beta-globulin preparations on the phase of fetal antigen recognition by sensitized lymphocytes from pregnant women. It was found that trophoblastic beta-globulin used in physiological concentrations does not exert an appreciable immunosuppressant effect on pregnant women's lymphocytes both in the first and third trimesters of pregnancy.

Beta-Globulins↗
PubMed1994

High continuing pregnancy rates after in vitro fertilization-embryo transfer using medium supplemented with a plasma protein fraction containing alpha- and beta-globulins.

OBJECTIVE: To evaluate both the laboratory and clinical outcomes after IVF-ET using culture media supplemented with a plasma protein fraction (PPF, Plasmatein; Alpha Therapeutics, Los Angeles, CA) containing albumin and significant amounts of alpha- and beta-globulins. DESIGN: One-year clinical trial of a PPF with high globulin content as a medium supplement during IVF, embryo growth, and ET. SETTING: Fertility Center of San Antonio, a private, office-based center for assisted reproduction. PATIENTS: Ninety-eight couples, with women ranging in age from 26 to 46 years, undergoing 103 ovum retrievals for IVF-ET as treatment for infertility because of tubal factor, endometriosis, anovulation, uterine or cervical factor, male factor, and unexplained causes. MAIN OUTCOME MEASURES: Fertilization rate, zygote cleavage rate, clinical pregnancy rate (PR), continuing PRs, and implantation rates. RESULTS: Supplementation with PPF in insemination, growth and transfer medium resulted in a clinical PR of 41.5% per transfer with continuing PRs of 35.2% per retrieval, 37.2% per patient, and 38.7% per transfer. CONCLUSIONS: A PPF containing significant amounts of alpha- and beta-globulins can serve as an effective protein supplement to IVF medium, with outcomes manifested as high continuing PRs. These data indicate a potential role for glycoprotein components of serum in supporting healthy embryo growth in vitro, although the mechanism may relate more to the general physicochemical properties of this fraction than to the actions of a specific component.

Adult↗
PubMed1981

Absence of beta-globulin band in the serum protein electropherogram of a patient with liver disease.

Agarose-gel electrophoresis of serum of a 72-year-old woman with liver cirrhosis showed virtually no beta-globulins two weeks before the patient's death. There was marked decrease in the concentrations of transferrin, beta-lipoproteins, hemopexin, complement component C3, beta-glycoprotein I, and cholesterol in serum. Absence of a beta-globulin band appears to signify an ominous prognosis.

Aged↗
PubMed1983

[Elevation of the serum concentration of alpha- and beta-globulins and of a particular carcinoembryonic antigen by ultrasound exposure of liver tissue and CEA-active carcinomas].

The concentration of alpha- and beta-globulins in the serum can be increased by exposure of the globulin-producing tissue of the liver to ultrasound. The ultrasound intensities producing this effect are harmless according to our present knowledge. Especially the concentration of the globulin carcinoembryonic antigen in the serum can be increased significantly by exposure of CEA-producing carcinomas to ultrasound. There is to investigate the possibility of the early detection of CEA-producing tumors using a provocation test by way of increasing still normal spontaneous CEA-concentrations in the serum by insonation of body regions in question to significant pathologic serum concentrations.

Alpha-Globulins↗
PubMed1990

[The immunochemical identification of beta-globulin in human seminal plasma].

Using affinity chromatography there was isolated a protein from human seminal plasma, which was named beta-globulin of seminal plasma (beta-GSP). It was demonstrated, that beta-GSP possesses heterogeneity, consists of two subunits with the M. W. about 19 and 15 kD, it has electrophoretic mobility of b1-globulin and possesses affinity to immobilized 17-beta-estradiol. Relatively high concentration of beta-GSP was found in seminal plasma. Besides, this protein was determined in small amounts in saliva. One can suppose, that beta-GSP is a secretory protein.

Animals↗
PubMed1999

Immunoprecipitation against exogenous antigens in the zone of alpha-1, alpha-2 and beta-globulins--a new immune phenomenon?

According to data by H. Zhabilov serum fractions in the zone of alpha 1, alpha 2 and beta-globulins from healthy people form a characteristic precipitation curve in terms of structure and density with the TNP (thymus nuclear protein) and CNP (carcinoma nuclear protein) as antigens when tested in two-dimensional agarose-gel electrophoresis. The observed immune phenomenon has not so far, to the best of our knowledge, been reported in the scientific literature and has profound implications for fundamental and applied immunology. The object of the present study was to confirm Zhabilov's data and study the ontogenetic aspect of the problem. We used 45 sera of children from different age groups, of healthy adult controls, HIV-infected and cancer patients. The sera were tested against the following exoantigens: TNP (thymus nuclear protein), CNP (carcinoma nuclear protein-Viral Genetics Inc, LA), as well as against ST (staphylococcal toxin), SL (streptolysin) and LPS (lipopolysaccharide) from Gram-negative bacteria (National Center for Parasitic and Infectious Diseases, Sofia, Bulgaria). Two-dimensional electrophoresis was used modified after the protocol of UCLA chemical labs. The results of our study show that immediately following birth the precipitation curve is barely discernible reaching the normal shape following 1 month-5 years of age. The precipitation curve from HIV-infected sera and those of cancer patients is similar to that of newborns. The results from our unpublished observations with sera from other biologic species tested against the same antigens show similar results: no curve was observed in fish whereas the reaction was positive with frogs, birds, rabbits and other mammals. This confirmation of the basic biologic law of ontogenesis being a repetition of phylogenesis gives us reason to consider those fractions as participating in the immune maturation of organisms for acquired immune response. There exists a possibility that they might be part of the already known immunomediators or a stage in the transition from non-specific to specific immunoglobulins which have not lost their importance in immunogenesis. The lack of previous studies in this respect and the observation and confirmation of this reaction in two different labs underscore the importance of this new immune phenomenon.

Adult↗