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PubMed1993

[Studies on oxygen content of aqueous humor in the anterior chamber of rabbit's eye--II. Oxygen content of aqueous humor in anterior chamber and arterial blood in experimentally provoked ischemia].

The ligature of the right common carotid artery has been performed in 20 rabbits. Then, the partial pressure of oxygen pO2 in the aqueous humor of anterior chamber of the both eyes and in the right and left ear artery was measured. An electrochemical polarographic oxymeter was used for this purpose. The measurements were carried out on the second day, in a week and in one month after performing the ligature. A statistically significant decrease of pO2 in aqueous humor of anterior chamber of the right eye and of ear artery on the right side was observed, especially on the second day and after a week. Blood circulation stoppage within the right common carotid artery causes, in spite of the preservation of collateral circulation, a significant pO2 decrease in aqueous humor. The pO2 measurement would be a valuable supplement to the methods applied in the measurement of ear artery blood circulation.

Animals↗
PubMed1993

[Studies on oxygen content in aqueous humor of the anterior chamber of the rabbit's eye--I. Oxygen content in aqueous humor of anterior chamber and arterial blood under physiologic conditions].

The measurements of the partial pressure of oxygen pO2 in the aqueous humor of anterior chamber in both eyes and arterial blood, using the electrochemical--polarographic method, under physiological conditions, have been carried out in 20 rabbits. The mean value of pO2 oxygen partial pressure in aqueous humor was 8.48 kPa, whereas in arterial blood was higher--9.69 kPa. The processes of infiltration (diffusion) from blood-vascular system and oxygen distribution in the aqueous humor of anterior chamber of the eye have been discussed.

Animals↗
PubMed1990

Rate of flow of aqueous humor determined from measurements of aqueous flare.

Measurement of light scattered in the anterior chamber can provide important clinical information about the eye. In this study, a scanning ocular spectrofluorophotometer was used to measure scattering in the anterior chamber. Pathologic scattering (flare) was induced by argon laser photocoagulation of the iris in pigmented rabbits. The spectrally corrected intensity of the flare in inflamed eyes decreased as the inverse 2.2 power of the wavelength of the illuminating beam. The strongest signal with this instrument was measured at 470 nm. Diurnal variation of normal and spontaneous scatter from the aqueous humor was measured in nine pigmented rabbits entrained to a 12-hr light/12-hr dark cycle and in eight human subjects. A technique is described for determining the rate of flow of aqueous humor from scattered light when the transfer rate of scattering substance into the anterior chamber is constant. This technique was used to study changes in flow over the diurnal cycle. The rate of flow in human subjects during sleep was 60% of the rate during waking hours. In rabbits, the rate was lowest (44% of maximum) during the 6 hr after the lights were turned on, and were greatest just before the lights were turned on. These findings confirm previous studies of the circadian rhythm of aqueous humor flow in rabbits and humans.

Animals↗