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Profiles of heterophile antibody to various mammalian erythrocytes in rural populations of Zambia.

Heterophile antibodies to sheep red blood cells (SRBC), rabbit red blood cells (RRBC), horse red blood cells (HRBC), murine (rat) red blood cells (MRBC) were investigated in a total of 416 serum samples from two rural areas of Zambia where major parasitic diseases are endemic. Distribution of antibody titers to RRBC were significantly different between the two study areas, whereas titers to SRBC were not significantly different. Sheep RBC's did not reveal a uniform consistent difference between the two communities with respect to schistosomiasis and malaria. Rabbit erythrocytes showed significant differences (P less than 0.05) between parasitologically positive and negative samples for schistosomiasis and malaria, respectively. No significant difference was observed between sexes, although females generally have a slightly higher titer for both SRBC and RRBC than males. No significant agglutinins were detected to HRBC and BRBC. Agglutinins to MRBC were generally too high to be of any differential value. It is suggested that sheep and rabbit erythrocytes might be useful probes for evidence of exposure to parasites and assessment of humoral immunologic status, albeit nonspecific. Further, it is inferred that the incidence of infectious mononucleosis in this region is rather low. A controlled investigation into the distribution of E-B viruses is suggested to explain absence of I-M-like agglutinins in a region climatically conductive to existence of arborviruses in general, and the E-B virus group in particular.

Adolescent↗

Prevalence of antibodies to West Nile fever, sandfly fever Sicilian, and sandfly fever Naples viruses in healthy adults in Israel.

BACKGROUND: The arbovirus infections West Nile fever (WNF) and sandfly fever (SFF) are both endemic in the Middle East. Despite the fact that the mosquito and sandfly vectors of these viruses are still found in Israel, SFF has not been reported since 1948, whereas outbreaks of WNF occur periodically. It is suspected that some of the cases of nonspecific febrile illnesses in Israel may in fact be either WNF or SFF. AIM: To determine the extent of past arbovirus infections in subgroups of Israeli soldiers. METHODS: Sera from random samples of three groups of healthy soldiers aged 18-20 (n=273), 21-30 (n=497), and 40-55 years (n=285) were examined for IgG and IgM antibodies to West Nile virus (WNV), sandfly fever Sicilian virus (SFSV), and sandfly fever Naples virus (SFNV), using an ELISA method. RESULTS: The prevalence of antibodies increased with age for all three viruses examined, and ranged from 7.0%, 0, and 2.8%, for WNV, SFSV, AND SFNV, respectively, in soldiers aged 18-20 years, to 41.9%, 23.7%, and 30.8%, in those aged 40-55 years. The percentage of seropositives for IgG who were also IgM positive were 1.0%, 5.9%, and 3.4%, for WNV, SFSV, AND SFNV, respectively. 17.5% of the seropositives for WNV were also positive for SFSV, as compared with 5.7% among the WNV-seronegatives. Among the seropositives for WNV, 23.99% were positive for SFNV, compared with 9.1% of WNV seronegatives. 48.2% of seropositives for SFSV were positive for SFNV, as compared with 8.4% for SFSV-seronegatives. In the age agroup 40-55 years, there were significantly higher prevalence rates in those born in Israel compared with those born abroad. CONCLUSIONS: There is still significant exposure to WNV, SFSV, and SFNV in Israel. Arborviruses are probably responsible for many cases of non-specific febrile illness in this region.

Adolescent↗

Classification, distribution and importance of arboviruses.

This essay reviews in concise form an extensive subject. Arborviruses belong to a number of different taxonomic groups, some of which include viruses not transmitted by arthropods. A state of the art definition of arboviruses is given which does recognise the importance of vertical transmission in arthropods to the basic maintenance of some arboviruses. Most of the arboviruses which affect humans are included in the families Togaviridae, Flaviviridae, Bunyaviridae, Reoviridae and Rhabdoviridae. Many arbovirus infections are symptomless. Clinical manifestations range from mild febrile illness, which may or may not be accompanied by skin rash and by arthralgia, to severe and often fatal encephalitis or haemorrhagic fever with shock. Three arboviral diseases considered at present as the most important ones are discussed in more detail: dengue and dengue haemorrhagic fever (DHF), yellow fever and Japanese encephalitis (JE). They have caused some of the most devastating epidemics in recent years. DHF has developed into a major paediatric problem in South East Asia and the Western Pacific with over 6,000,000 hospital admissions and 20,000 deaths in the two regions over the last 20 years. Spectacular outbreaks of yellow fever, such as the one in Ethiopia in 1960-1962 with 15,000-30,000 estimated deaths, still occur in Africa in areas contiguous to rain forest regions where jungle yellow fever is enzootic. JE is characterized by significant mortality in children and old people in many countries of Asia, where the epidemiological patterns and the distribution of the disease have changed in recent years. The complex interrelated factors which are involved in arbovirus ecology are illustrated.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Serological studies on sandfly fevers in the Republic of Bangladesh.

Blood samples from 160 inhabitants of the Republic of Bangladesh were studied by haemagglutination inhibition (HI), indirect HI, and radial haemolysis in gel tests. The sera were found to contain antibodies to the arborviruses of Sicilian (6.25%) and Naples (1.25%) sandfly fevers and to the Karimabad virus (11.25%) which are transmitted by Phlebotomus papatasi. Antibodies to the Karimabad virus were found among the Bangladesh population for the first time.

Antibodies, Viral↗