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Effect of water pollutants and other chemicals upon the activity of lipase in vitro.

Lipase preparations were treated in vitro with 100 chemicals of various classes, many of which are environmental pollutants, to determine their effect upon enzyme activity. The greatest inhibition was caused by mercuric ion and certain heavy metal cations; almost as inhibiting were pentachlorophenol, dicofol, 2,4,5-trichlorophenol, and DDT. Less inhibition was found with other inorganic salts, organo-metals, other organic chemicals, and some pesticides. Apart from dichromate ion, the anions studied had a low to negligible effect on lipase. A mixture of 10 inorganic salts had an approximately cumulative inhibitory effect on enzyme activity. None of the chemicals studied caused a measurable activation of the enzyme.

Lipase↗

Expression and inducibility of members in the cytochrome P4501 (CYP1) family in ringed and grey seals from polluted and less polluted waters.

The expression and inducibility of cytochrome P4501A (CYP1A) were investigated in hepatic microsomes of ringed seals (Phoca hispida) and grey seals (Halichoerus grypus) from polluted (Baltic Sea) and less polluted (Svalbard and Sable Island) areas. Liver CYP1A protein levels and activities were assessed by immunoblot analysis and determining catalytic activities towards ethoxyresorufin (EROD) and pentoxyresorufin (PROD). The amount of CYP1A apoprotein and its catalytic activity were increased up to 5-fold in Baltic ringed and grey seal populations in comparison with ringed seals from Svalbard and grey seals from Sable Island. EROD and PROD activities correlated in all seal groups, indicating catalysis by the same CYP form(s). Enzyme kinetic studies suggested that PROD activity is catalysed by CYP1A enzymes in both ringed and grey seals. In immunoblot analysis, a protein was revealed in liver with an antibody against human CYP1B1, indicating that a CYP1B1 like protein could be present in ringed and grey seals. In conclusion, these data strengthen the concept that CYP1A is markedly induced in seals living in polluted waters and that both EROD and PROD activities are mediated by CYP1A forms in the seal liver. In addition, this study provides the first evidence for the presence of a CYP1B like protein in seal liver.

Journal Article↗

[Water pollution and its impact in the Basic Health Area of Cistierna].

OBJECTIVE: The purpose of this study was the quantitative and qualitative analysis of the water in the reservoir and supply system to 27 villages of over 100 inhabitants or those which had a village council, within the Cistierna Health Area. DESIGN: It was a descriptive, longitudinal, prospective and observational study. We chose certain villages for the number of their inhabitants and where water problems might have more serious effects. Medical personnel, biologists, chemists and primary care ATS-DUE (nursing staff) took part in the study. INTERVENTIONS: The villages were divided into 5 groups. Water was collected at each one on the same day of every month by the same staff. Analysis went on without interruption for the 12 months from February 1, 1991, to January 31, 1992. The water was analysed at the laboratory of the Health and Welfare Territorial Service (León). Beforehand, a survey on infrastructure had been administered to the staff of the 82 neighborhood or village councils making up the Basic Health Area. MEASUREMENTS AND MAIN RESULTS: None of the villages maintained chlorination for more than 8 months. The majority chlorinated for 2 months (11.11% of the Network). Coliforms were detected in an extremely high number of places. The majority of the samples contained a significant number--to a greater or lesser extent--of fecal coliforms. CONCLUSIONS: Infrastructure and maintenance of the water supply system is inadequate and insufficient. The final verdict from the analyses indicate that, in approximately half the samples taken in this rural area, the water was not drinkable neither in the reservoir nor in the supply network.

Adult↗

[Microbiological evaluation of water pollution in the Volga delta].

Bacterial contamination of water and fish in the Volga delta region was studied. Water near large enterprises contained more bacteria than at 75 km a distance of downstream. All organs and tissues of sturgeon contained opportunistic bacteria. Bacterial contamination of water is responsible incidences for acute intestinal diseases of the population.

Animals↗

Research and control of well water pollution in high esophageal cancer areas.

AIM: In order to detect risk factors for esophageal cancer, a national research program was carried out during the Eighth Five-Year Plan (from 1991 to 1995). METHODS: Cixian County and Chichen County in Hebei Province were selected as the index and the control for the study fields with higher or lower incidence of esophagus cancer in China, respectively. In these areas, we investigated the pollution of three nitrogenous compounds in well water for drinking and the use of nitrogen fertilizer in farming. RESULTS: In well water, nitrate nitrogen, nitrite nitrogen and ammonia nitrogen were 8.77 mg/L, 0.014 mg/L and 0.009 mg/L in Cixian County in 1993, respectively. They were significantly higher than their levels (3.84 mg/L, 0.004 mg/L and 0.004 mg/L) in Chichen County (P<0.01, t=6.281, t=3.784, t=3.775). There was a trend that the nitrogenous compounds in well water increased from 1993 to 1996. The amount of nitrogen fertilizer used in farming was 787.6 kg per hectare land in Cixian County in 1991, significantly higher than 186 kg per hectare in Chichen County (t=9.603, P<0.001). CONCLUSION: These investigations indicate that the pollution of nitrogenous compounds in well water for drinking is closely related to the use of nitrogen fertilizer in farming, and there is a significantly positive correlation between the level of three nitrogenous compounds in well water and the mortality of esophageal cancer (correlation coefficient =0.5992). We suggest that improvement of well system for drinking water quality should be an effective measure for esophageal cancer prevention and control in rural areas.

China↗

A comparative analysis: storm water pollution policy in California, USA and Victoria, Australia.

Urban drainage systems historically were developed on principles of hydraulic capacity for the transport of storm water to reduce the risk of flooding. However, with urbanization the percent of impervious surfaces increases dramatically resulting in increased flood volumes, peak discharge rates, velocities and duration, and a significant increase in pollutant loads. Storm water and urban runoff are the leading causes of the impairment of receiving waters and their beneficial uses in Australia and the United States today. Strict environmental and technology controls on wastewater treatment facilities and industry for more than three decades have ensured that these sources are less significant today as the cause of impairment of receiving waters. This paper compares the approach undertaken by the Environmental Protection Authority Victoria for the Melbourne metropolitan area with the approach implemented by the California Environmental Protection Agency for the Los Angeles area to control storm water pollution. Both these communities are largely similar in population size and the extent of urbanization. The authors present an analysis of the different approaches contrasting Australia with the USA, comment on their comparative success, and discuss the relevance of the two experiences for developed and developing nations in the context of environmental policy making to control storm water and urban runoff pollution.

California↗

Current developments in the analysis of water pollution by polychlorinated biphenyls.

A review and discussion of the analytical developments of the last 10 years in the determination of polychlorinated biphenyls (PCBs) in water are presented, including sample treatment, clean-up, identification and determination. Some of the most significant advances have occurred in the area of sample preparation, such as the increased adoption of solid-phase extraction and on-line techniques and the chromatographic separation of PCB congeners, principally by mass spectrometry. Special attention is focused on applications to real samples.

Chromatography, Gas↗

A comparison of isolation procedures for salmonellas from polluted water using two forms of Rappaport's medium.

The efficiency of Rappaport's broth ( RB10 ) and Rappaport's broth containing novobiocin ( NRB10 ) were compared for the isolation of salmonellas from polluted water, both as direct enrichment media and after pre-enrichment in buffered peptone water. Ninety samples were examined and 41 were found to contain salmonellas by at least one of the procedures used. Direct inoculation of the sample into RB10 resulted in the recovery of salmonellas from only 29.3% of the samples found to be positive. The use of NRB10 as a direct enrichment medium increased the percentage recovery to 78.0% of the total positive samples. Pre-enrichment in buffered peptone water allowed the recovery of salmonellas from a total of 41 samples whereas direct enrichment recovered them from only 32. No significant difference was demonstrated in the efficiencies of RB10 and NRB10 in recovering salmonellas after pre-enrichment in buffered peptone water. Three selective agars were used: no significant difference in their ability to recover salmonellae was demonstrated.

Culture Media↗