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Three-dimensional reconstruction of the chromatin bodies in the nuclei of mature erythrocytes from the newt Triturus cristatus: the number of nuclear envelope-attachment sites.

The arrangement of the chromatin bodies in the interphase nuclei of 6 erythrocytes has been investigated by means of 3-dimensional reconstruction from electron micrographs of serial sections. When the borders of chromatin bodies are marked on the surface of each model, discrete areas of chromatin in contact with the nuclear envelope are revealed. The number of these areas in approximately equal to the number of chromosomes in the diploid set. The data suggest that each chromatin body corresponds to a condensed interphase chromosome and that each chromosome is attached to one discrete site on the nuclear envelope. The data are insufficient to show whether or not the condensed chromosomes are arranged in any orderly pattern in these nuclei.

Animals↗

Innervation of two peptidergic (substance P and calcitonin gene-related peptide) nerves in the cerebral arteries and choroid plexus of the Japanese Newt (Triturus pyrrhogaster).

The pattern of cerebrovascular substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactive (-IR) innervation was investigated in the newt. SP-IR nerves supplying the cerebral arterial tree and choroid plexus were positive for CGRP, but negative for vasoactive intestinal polypeptide or neuropeptide Y. It is suggested that cerebrovascular SP- and CGRP-IR axons are sensory in nature. The supply of SP- and CGRP-IR nerves to the major cerebral arteries is relatively poor. Nevertheless, numerous SP- and CGRP-IR axons, which are contained in the fiber bundles on the cerebral carotid artery and the basilar artery, spread widely over the microvascular-epithelial regions of the choroid plexuses. It must be considered in relation to the significant role of SP- and CGRP-IR neuronal mechanisms responsible to the microcirculation, cerebrospinal fluid (CSF) production and transport action within the choroid plexus in the nutrition of the newt brain via the CSF.

Animals↗